Labshake search
Citations for Merck :
501 - 550 of 593 citations for Recombinant Human Complement Factor H His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... The concentrations of Aβ40 and Aβ42 were determined in brain lysates using the ELISA kits according to the manufacturer’s instructions (human Aβ40 and Aβ42 brain ELISA, Merck).
-
bioRxiv - Genomics 2022Quote: ... females and males were primed with 20 U and 10 U human chorionic gonadotropin (hCG, Pregnyl, Merck), respectively ...
-
bioRxiv - Immunology 2022Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density gradient centrifugation (GE-171440-02, Merck), collected from the interphase ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were sub-cultured on days 4 and 8 of differentiation onto human fibronectin (Merck Millipore, #FC010) coated plates at a ratio of 1:4 ...
-
bioRxiv - Biochemistry 2023Quote: ... Antibodies usedwere: mouse monoclonal (BIII-136) anti human Band 3 (B9277, Merck Life Science S.r.l., Milan, Italy). Mouse monoclonal (H68.4 ...
-
bioRxiv - Cell Biology 2023Quote: ... fully mature Xenopus females were injected with 260 U of human chorionic gonadotropin (hCG; Merck, Ovitrelle 250G) into the dorsal lymph sac about 12-16 h before use and kept overnight at 18 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... The cells were fixed for 1 h at room temperature with 4% buffered formalin solution containing 1% crystal violet (Merck, Darmstadt, Germany). Finally ...
-
bioRxiv - Neuroscience 2021Quote: ... PBS rehydrated tissue was then blocked using 10% normal horse serum in 0.2% Triton X-100 in PBS for ∼1 h and then primary antibodies specific for S100 (Merck, S2532, 1:200) TUJ1 (Synaptic systems ...
-
bioRxiv - Cell Biology 2019Quote: ... HeLa cells were allowed to adhere overnight (16 h) in the presence of 2 mM Thymidine (S-phase block; Merck, Darmstadt, Germany) and released into fresh medium ...
-
bioRxiv - Bioengineering 2021Quote: Inflammatory stimulations were performed by treating the chips for 24 h from d4-d5 or d11-d12 with TNF-α (final concentration of 20 ng/mL; SRP3177; Merck KGaA) or LPS (final concentration of 100 ng/mL ...
-
bioRxiv - Neuroscience 2022Quote: ... The pellets were lysed for 1 h using cell protein extraction reagent (CW BioTech, CW0889, China) with protease inhibitor Cocktail set III (1 %, v/v, Merck, 535140, Germany), followed by centrifugation at 500×g for 10 min and the supernatants were collected ...
-
bioRxiv - Neuroscience 2020Quote: ... 0.2% Tween-20 in PBS) for 1 h and then incubated with a rabbit antibody against the extracellular domain of CSF1R (Merck, 06-174,) or a mouse anti-HA tag antibody (BioLegend ...
-
bioRxiv - Cell Biology 2020Quote: ... The proteasome inhibitor MG132 was used at 1 µM for 24 h (stock solution: 10 mM in DMSO, 474791, Sigma Aldrich/Merck KGaA). For all inhibitors ...
-
bioRxiv - Neuroscience 2022Quote: ... ileum and colon sections were deparaffinated in histolene and rehydrated in a descending series of ethanol (100%, 96%, 70%) followed by hematoxylin solution (H&E, Merck, Darmstadt, Germany) for 5 min at room temperature ...
-
bioRxiv - Plant Biology 2022Quote: ... GSL in 1 ml crude extracts were bound on 250 µl DEAE-Sephadex A-25 columns and digested on the column for 18 h at room temperature with 18U sulfatase H1 enzyme (Merck KGaA, Germany). Desulfoglucosinolates were eluted twice with 1 ml ddH2O ...
-
bioRxiv - Biochemistry 2023Quote: ... samples of 8 μM aSyn in the aggregation buffer were incubated at 37 °C under quiescent conditions and over 20 h with and without a cylindrical 10 mm x 3mm Teflon stirring bar (Merck, ref. Z328650) or 0.5 μM oTTR ...
-
bioRxiv - Biochemistry 2022Quote: ... Culture supernatants were harvested 24 h post-viral infection and then passed through 0.22 μM pore-sized filters (Merck Millipore, Carrigtwohill, Ireland) to remove cell and debris and obtain viral suspension for experiments.
-
bioRxiv - Cell Biology 2023Quote: ... each sample was incubated for 12 h (or overnight) in bleaching solution containing hydrogen peroxide solution (H2O2; Cat. No. H1009; Sigma-Aldrich, Merck, Germany) at a final concentration of 15% (v/v)) ...
-
bioRxiv - Neuroscience 2020Quote: ... RIPA and FA fractions was assessed using the Human Aβ1-42 enzyme-linked immunosorbent assay (ELISA) Kit (Merck) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: Primary human macrophages (pMФ) were isolated from the whole blood of healthy volunteers with Biocoll separating solution (Merck) according to the manufacturer’s protocol and seeded at a density of 2×106 cells per well in a 6-well plate ...
-
bioRxiv - Biochemistry 2020Quote: ... Human Rab8a 6-176aa (referred to as Rab8a)-encoding DNA was cloned into a pet51b(+) vector (Merck Millipore), resulting in a construct with a N-terminal Strep® II tag and enterokinase cleavage site and a C-terminal 10xHis tag ...
-
bioRxiv - Biochemistry 2020Quote: ... at increasing concentrations (3.8 pM–250 nM) in the presence of 90% heat-inactivated human serum (H5667, Merck) and incubated at 4 °C overnight ...
-
bioRxiv - Microbiology 2021Quote: ... The human macrophage-like cell line U937 (ATCC no. CRL-1593.2) was cultured in RPMI-1640 medium (Merck) supplemented with 10% iFCS ...
-
bioRxiv - Cell Biology 2021Quote: Human hTERT RPE-1 cells (ATCC, CRL-4000) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM/F12, Merck) supplemented with 10% fetal calf serum (FCS) ...
-
bioRxiv - Bioengineering 2022Quote: ... Human brain endothelial cell line (hCMEC/D3) and EndoGRO™ MV cell medium kit were purchased from Merck-Millipore (MA ...
-
bioRxiv - Cell Biology 2023Quote: Glass bottom well plates (Celvis) were coated with 5 µg/ml of human plasma fibronectin purified protein (Merck) for one hour at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: Glass bottom well plates (Celvis) were coated with 5 µg/ml of human plasma fibronectin purified protein (Merck) for one hour at room temperature ...
-
bioRxiv - Genomics 2023Quote: Luminex assays were performed using the Milliplex Human Cytokine/Chemokine Magnetic Bead Panel (HCYTOMAG-60K-41, Merck Millipore) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: ... spermatozoa from JF1/MsJ males were capacitated in Human Tubal Fluid medium (Merck Millipore, Cat#MR-070-D) supplemented with 10 mg ml−1 Albumin (Sigma ...
-
bioRxiv - Immunology 2023Quote: ... Cells were cultured for 14 days in RPMI 1640 (ccpro) supplemented with 10 % heat-inactivated human serum (Merck), 10 ng/ml IL-7 ...
-
bioRxiv - Developmental Biology 2024Quote: ... spermatozoa were released into 500 μL of preincubated human tubal fluid medium (HTF: Millipore, Merck KGaA, Darmstadt, Germany) for 10 min at 37°C ...
-
bioRxiv - Cell Biology 2024Quote: ... cover slips were incubated with the primary antibody (SV40 T antigen mouse-anti-human, Merck-Millipore, 1:50) overnight at 4 °C in a humidifier chamber ...
-
bioRxiv - Neuroscience 2019Quote: ... sections were incubated for 2 h at room temperature with the following affinity pure primary antibodies: rabbit polyclonal anti-TRPM4 (Cat.# ABN418; Merck Millipore, Darmstadt, Germany) and mouse monoclonal anti-Aquaporin 4 antibody [4/18] (ab9512 ...
-
bioRxiv - Physiology 2023Quote: ... they were incubated in their respective secondary antibodies for 1 h at room temperature (goat anti-rabbit, IgG, HRP conjugated; Merck Millipore (1: 5000) in 5% milk-TBST ...
-
bioRxiv - Molecular Biology 2023Quote: ... 20 15 cm dishes were seeded and the nuclear translocation of SAMD1 was induced 24 h before extract preparation by adding 200 nM 4-OHT (Merck; 68392-35-8). After collection ...
-
bioRxiv - Plant Biology 2023Quote: ... The sections were then incubated for 1 h with 700 µL of a 10% (v/v) DMSO/3% (v/v) IGEPAL® CA-630 (Merck, Darmstadt, Germany) in MTSB ...
-
bioRxiv - Biochemistry 2023Quote: ... membrane using wet transfer for 3 h at 90 V/4 °C in transfer buffer (25 mM Tris; 192 mM glycine, Merck; 20% methanol, Merck). Afterwards ...
-
bioRxiv - Molecular Biology 2024Quote: ... Approximately 1,500 worms per replicate were harvested at 12 h post L4 and washed across a Steriflip® 20 μm nylon mesh filter (Merck; Darmstadt, Germany) with M9 buffer ...
-
bioRxiv - Physiology 2020Quote: ... Insulin concentration was determined using the MILLIPLEX® MAP Human Metabolic Hormone Magnetic Bead Panel (Merck KGaA, Darmstadt, Germany) according to the manufacturer s instructions.
-
bioRxiv - Cell Biology 2021Quote: ... Purified human integrin α5β1 and anti-α5 (MAB1956Z) and anti-α5β1 (MAB1999) antibodies were from Merck (Kenilworth, NJ, USA). Volociximab was from Novus Biologicals (Littleton ...
-
bioRxiv - Cell Biology 2021Quote: The digoxigenin-labeled antisense probe targeting bases 32-500 of human GNRH1 mRNA (NM_001083111.2) was transcribed in the presence of digoxigenin-11-UTP (Merck Millipore) in a reaction mixture containing linearized cDNA template (1 µg) ...
-
bioRxiv - Biochemistry 2020Quote: ... was equilibrated with increasing concentrations of NAb (30 pM–1.0 µM) in the presence of 90% heat-inactivated human serum (H5667, Merck) at a temperature of 4 °C overnight ...
-
bioRxiv - Biochemistry 2020Quote: ... was mixed with unlabeled SARS-CoV-2 spike S1 at increasing concentrations (100 pM–3.2 µM) in the presence of 90% heat-inactivated human serum (H5667, Merck) and incubated at 4 °C overnight ...
-
bioRxiv - Physiology 2019Quote: ... The hPCLS were treated with 10ng/ml recombinant human TGFß 1 protein (R&DSystems#240-B-010) and 25µM (in 750µl of DMEM) of GM6001 (Merck#CC1010). Lived dead staining was carried out using calcein-am/ ethidium homodimer kit (ThermoFisher#L3224 ...
-
bioRxiv - Molecular Biology 2022Quote: ... CD3+ T cells were cultured in TexMACS Medium (Miltenyi Biotech) supplemented with 5% heat-inactivated human AB serum (Merck), 100 units/mL penicillin (Merck ...
-
bioRxiv - Molecular Biology 2022Quote: ... and primers anchored in the homology arms of the HDRT specific for KI at the human TRAC locus (Merck). PCR amplicons (typically 2.5 mL ...
-
bioRxiv - Cell Biology 2023Quote: 293T human embryo kidney (HEK293T) cells were grown in Dulbecco’s modified Eagle’s medium (4.5 g/l glucose) (Merck, Dorset, UK) enriched with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Biophysics 2021Quote: ... Cells were then incubated for 1 h at room temperature with the monoclonal mouse Anti-Tom20 (1:500, MABT166, Merck & Co., Kenilworth, NJ, USA). The antibody was revealed using Atto 594 goat anti-mouse IgG (1:500 ...
-
bioRxiv - Developmental Biology 2022Quote: ... MII oocytes were activated for 6 h in Ca-free α-MEM medium containing 10 mM SrCl2 and 5 μM Latrunculin B (cat. no. 428020, Merck Millipore, Darmstadt, Germany). Following activation ...
-
bioRxiv - Microbiology 2021Quote: ... PBMCs (2 × 106 cells) were plated onto 48-well plates (NalgeNunc) in RPMI-1640 with 5% inactivated male human AB serum (Merck) for 3 hours ...