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Citations for Merck :
351 - 400 of 616 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... All cell lines were tested every 6-12 months either by PCR (LookOut kit, Sigma/Merck) or Mycostrips (Invivogen ...
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bioRxiv - Cell Biology 2024Quote: ... The full-length MDM2 coding sequence was isolated from Addgene plasmid #16233 (pcDNA3 FRT MDM2) by PCR using the KOD hot start polymerase kit (Merck #71086), using primers that included a 5’ BamHI restriction site ...
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bioRxiv - Molecular Biology 2024Quote: ... Each PCR included a negative control using Milli-Q water (Merck; Rahway, New Jersey, United States) instead of template DNA ...
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bioRxiv - Cell Biology 2020Quote: ... The water used was of Milli-Q Advantage A10 as well as MultiScreen-HV 96-well Plates (Merck Millipore). Calibration ranges were 3.125 – 200 ng/ml for U and 25 - 500 ng/ml for UH2 and 5-FU (5μg/ml ...
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bioRxiv - Microbiology 2019Quote: ... Aliquots of neutral lipid extracts were analyzed by thin layer chromatography (TLC) on 60 F254 silica gel plates (Merck) using hexane/diethyl-ether/acetic-acid (90:8:1 ...
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bioRxiv - Immunology 2021Quote: ... The plates were then washed 5 times with HSWB followed by incubation of goat anti-mouse antibody (Merck, Germany) conjugated with HRP (diluted 1:3000 in LSWB + 1% BSA ...
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bioRxiv - Microbiology 2019Quote: ... The steroid standards and products were separated on silica gel-coated aluminum TLC plates (Silica gel 60 F254: thickness, 0.2 mm; 20 × 20 cm; Merck) using dichloromethane:ethyl acetate:ethanol (14:4:0.05 ...
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bioRxiv - Microbiology 2019Quote: ... 1 μL of the reaction was spotted onto aluminium backed silica gel plates (5735 silica gel 60 F254, Merck) and dried before being subjected to Thin Layer Chromatography (TLC ...
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bioRxiv - Immunology 2021Quote: ... (final concentration of 2µg/mL) and pools of original or beta variant peptides on hydrophobic-PVDF ELISpot plates (Merck) coated with 5µg/mL anti-mouse IFNγ (AN18) ...
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bioRxiv - Biochemistry 2020Quote: ... Reaction mixtures were stirred using Teflon-coated magnetic stirrer bars and monitored by thin layer silica gel chromatography (TLC) using 0.25 mm silica gel 60F plates with fluorescent indicator from Merck. Plates were visualized under UV or treated by KMnO4 stain with gentle heating ...
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bioRxiv - Cell Biology 2021Quote: ... and/or with plasmid DNA (e.g. 1µg/well for 6-well plates) for 24 hrs using GeneJuice (Merck Millipore). siRNAs targeting USP7 ...
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bioRxiv - Microbiology 2021Quote: ... Polar lipids were separated by two-dimensional thin layer chromatography (TLC plates coated with silica gel, 1010 cm; Merck). Chromatography was performed using chloroform/methanol/water (65 ...
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bioRxiv - Cell Biology 2021Quote: ... Growth assays on solid medium were done as described (Schuck et al., 2009) using SCD plates with and without 0.2 µg/ml tunicamycin (Merck). Plates were imaged after 1.5 days of growth at 30°C.
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bioRxiv - Neuroscience 2022Quote: ... Cells were cultured on poly-L-ornithine and laminin-coated coverslips in 24-well plates at 150,000 cells per well in serum-free 2i Medium (Merck). After 24h ...
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bioRxiv - Cell Biology 2022Quote: ... and seeded in 48-well plates (3000-4000 cells/well) previously coated with 0.1mg/ml poly-D-lysine (2h, 37°C; Merck) and 2μg/ml laminin (over-night (O/N) ...
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bioRxiv - Biochemistry 2020Quote: ... was immobilized into the membrane pores (0.45 μm pore size) of a 96-well MultiScreen-IP polyvinylidene fluoride (PVDF) filter plate from Merck Millipore (Figure 2B) ...
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bioRxiv - Microbiology 2019Quote: ... The pellet was dissolved in three ul distilled water and spotted onto 5×5 cm silica gel plates (Merck) using automatic TLC sampler ...
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bioRxiv - Developmental Biology 2019Quote: ... Silica gel 230-400 mesh and silica gel coated plates Kieselgel 60 F254 were purchased from Merck (Darmstadt, Germany) and were used for flash-chromatography (FC ...
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bioRxiv - Microbiology 2020Quote: The TLC was performed by spotting 2 μL of enzymatic reaction on a silica gel 60 F454 plate (Merck), the separation was carried out in butanol ...
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bioRxiv - Microbiology 2019Quote: ... and 5 µl aliquots of the supernatants were applied to polyethyleneimine (PEI) cellulose thin layer chromatography plates (Merck, Sigma), resolved with 1.5 M KH2PO4 (pH 3.4 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Unreacted dye and SMA-SH were removed by thin layer chromatography (TLC) using silica gel plates (Merck, Darmstadt, Germany) and a mobile phase of chloroform/methanol/water (5:5:1 v/v/v) ...
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bioRxiv - Microbiology 2020Quote: ... after which the plates were placed inside a 2.5 L sealed jar (BBLTM GasPakTM from BD Biosciences or from Merck) under micro-aerobic conditions created by using an oxygen-absorbing AnaeroGen sachet (Oxoid) ...
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bioRxiv - Microbiology 2020Quote: ... room-temperature and slightly dried SabCG media plates and a sterile glass L-shaped bacteriology cell-spreader (Merck, S4522) was used to spread the liquid evenly over the entire surface and allowed to soak/dry before use.
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bioRxiv - Microbiology 2021Quote: ... An aliquot was subjected to TLC using a silica gel 60 F254 aluminum TLC plate (Merck Millipore, cat.no.105554,) with a running buffer containing ethyl acetate/acetic acid/methanol/formic acid/water in a ratio of 80:40:10:10:10 (v/v) ...
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bioRxiv - Pathology 2021Quote: ... 96-well plate was coated with 1 μg/ml monoclonal anti-lubricin antibody clone 9G3 (Merck Millipore, Billerica, MA) in TBS at 4°C over-night ...
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bioRxiv - Microbiology 2021Quote: ... Isolated lipids were dissolved in 30 μL of chloroform:methanol (2:1, v/v) and 5 μL loaded on thin-layer chromatography (TLC) silica gel plates F254 (Merck). Lipids were separated in chloroform/methanol/water (20:4:0.5 ...
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bioRxiv - Microbiology 2019Quote: ... An inoculum from the SDA plate was suspended in 25 ml of Sabouraud dextrose broth (SDB; Merck, Darmstadt, Germany) and incubated for 18 h under agitation (120 rev/min ...
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bioRxiv - Microbiology 2019Quote: ... Reaction products were then visualized by spotting 1 μl on PEI-cellulose thin layer chromatography (TLC) plates (Merck Millipore) and separated using 1 M KH2PO4 ...
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bioRxiv - Microbiology 2020Quote: ... 96-well plates with confluent cell layers were fixed and virus was inactivated by immersion in 100% methanol (MERCK) for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... The dried pellets were dissolved in 80 % methanol and analysed by HPTLC on a Silica Gel 60 plate (Merck). Ethyl acetate ...
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bioRxiv - Microbiology 2021Quote: ... Five lactose positive colonies were selected form the plate and β-glucuronidase activity was confirmed using Chromocult Agar (Merck).
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bioRxiv - Immunology 2021Quote: Viral vectors were produced by triple transient transfection of HEK-293Ts in 100 mm plates using GeneJuice (Merck – 70967) with a total of 12.5 μg of DNA ...
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bioRxiv - Microbiology 2020Quote: U-2 OS cells were seeded to 80% confluency in a 12-wells plate and washed twice with HNE supplemented with 0.5 mM CaCl2 (Merck), 0.5 mM MgCl2 (Merck ...
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bioRxiv - Microbiology 2022Quote: The steroid standards and products were separated on a silica gel-coated aluminum TLC plate (Silica gel 60 F254: thickness, 0.2 mm; 20 × 20 cm; Merck) using dichloromethane:ethyl acetate:ethanol (14:4:0.05 ...
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bioRxiv - Plant Biology 2022Quote: ... and selection of primary transformants (T1) was done on MS-agar plates supplemented with glufosinate-ammonium (Merck, Darmstadt, Alemania) (10 mg/L) ...
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bioRxiv - Plant Biology 2023Quote: ... dried and transferred in a grid pattern onto square plates containing 50 ml of MS/2 medium (0.5× Murashige and Skoog salts [Merck Sigma-Aldrich] ...
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bioRxiv - Microbiology 2022Quote: ... The dilutions were plated on nutrient broth agar (NA) plates containing 0.04% L-arabinose using a replica plater (Merck). After drying the plates were incubated for 16 h at 30°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The Lipid II extract was assessed by spotting 1-2 μL on a HPTLC silica gel 60F254 plate (Merck). The TLC plate was developed in a mixture of chloroform:methanol:water:ammonia (88:48:10:1 ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were plated (12-well plate) on glass coverslips coated with poly-L-lysin-hydrochloride (1 mg/ml, Merck). After 2h ...
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bioRxiv - Microbiology 2023Quote: ... the lipids were resuspended in methanol:chloroform (1:1) and subjected to thin layer chromatography (TLC) using silica gel 60 plates (Merck). Total lipids were separated using chloroform:methanol:water (65:25:4 ...
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bioRxiv - Microbiology 2023Quote: ... spotted in Thin-layer chromatography (TLC) silica plate coated with the flourescent indicator F254 (Merck Millipore, Burlington, MA, USA), and resolved in TLC plates containing toluene:ethyl acetate:acetic acid (8:1:1 ...
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bioRxiv - Biochemistry 2023Quote: ... 3 x 105 cells per well were seeded into a 6-well plate in high-glucose Dulbecco’s modified Eagle’s (DMEM) medium (Merck, UK) enriched with 10% FBS (Sigma- Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... Single colonies from those plates were inoculated in 5 mL of tryptic soy broth (TSB; Merck KGaA, Darmstadt, Germany) and incubated for 16 - 18 h at 37°C with shaking at 200 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... and lysed in 250µL/plate of RIPA buffer (89901) supplemented with EDTA and protease (78430) and phosphatase (Merck, 524625) inhibitor cocktails ...
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bioRxiv - Systems Biology 2023Quote: ... For the multicellular spheroid generation 7.000 cells were seeded on ultra-low attachment 96-well plates (Corning CoStar, Merck). The spheroids were cultured for 72h at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... RH5ΔNC-specific B cells were identified as live CD19+ IgG+ RH5ΔNC-APC+ RH5ΔNC-PE+ cells and single cell sorted into 96-well plates containing 10µL/well lysis buffer (10mM Tris [T3038, Merck] ...
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bioRxiv - Plant Biology 2023Quote: ... media plates were infused with sterile-filtrated 45 ml 2x PEG-6000 (Merck group; dissolved in liquid ½ MS media) for exactly 24 h at RT and afterwards the PEG-solution completely decanted ...
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bioRxiv - Immunology 2024Quote: ... 50 μL/well of plex beads were distributed in a MultiScreenHTS BV Filter Plate (1.2 μm Hydrophilic Low Protein Binding Durapore Membrane, Merck) and washed three times with 120 μL of ABE buffer ...
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bioRxiv - Plant Biology 2023Quote: ... The extract was separated on TLC plates (TLC aluminum sheet of silica gel 60, Merck, Whitehouse Station, NJ, USA), which were developed with cyclohexane:ethyl acetate (1:1 ...
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bioRxiv - Neuroscience 2024Quote: Thin-layer-chromatography (TLC) was performed on 0.25 mm Silica-Gel 60 F plates with a 254nm fluorescence indicator from MERCK. The substance detection took place by light with 254nm and 360nm wavelengths ...