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Citations for Merck :
151 - 200 of 488 citations for P selectin Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore, Massachusetts, USA) and the membranes were blocked with PBS containing 5% non-fat dry milk for 30 min ...
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bioRxiv - Biophysics 2022Quote: ... and 1% penicillin/streptomycin (P/S) Solution (P0781, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany), in an environment with 5% CO2 (v/v ...
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bioRxiv - Plant Biology 2024Quote: ... The separated proteins were transferred to Immobilon®-P PVDF membrane (Merck Millipore, USA) and probed with primary antibodies overnight ...
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bioRxiv - Bioengineering 2019Quote: ... human fibronectin (Merck-Millipore, Watford, UK) was premixed with Laponite gels to a final concentration of 50 µg/mL ...
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bioRxiv - Microbiology 2019Quote: ... Binding of human FH (Merck Millipore) was detected with Alexa-Fluor-488 labeled human FH ...
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bioRxiv - Immunology 2022Quote: ... Purified C1q from human serum (Merck) was used as a control.
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bioRxiv - Systems Biology 2020Quote: ... anti-human MLKL (Merck Millipore, MABC604), phospho anti-human RPB1 S2 (Millipore ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... Human plasma full-length fibronectin (Merck) was added to the dish at a final concentration of 10 μg/μl for a 1h incubation at 37°C ...
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bioRxiv - Immunology 2023Quote: Human IFNβ (IF014, Merck, KGaA, Darmstadt), IFNα2a (11100-1 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5 μg/ml human insulin (Merck), and 50 μM hydrocortisone (Cayman ...
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bioRxiv - Immunology 2024Quote: ... Human MC lines HMC-1.1 (Merck) and HMC-1.2 (Merck ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was then incubated with 2 mL pre-washed HIS-Select Cobalt Affinity Gel (Merck, UK) with rotation for an hour ...
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bioRxiv - Biochemistry 2023Quote: His-tagged proteins were labeled using Ni-NTA-ATTO 550 or Ni-NTA-ATTO 647 from Merck Group ...
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bioRxiv - Genetics 2023Quote: ... imidazole was removed from the eluted His-SIRT5 using ultra-0.5 centrifugal filter units (UFC501096, Merck Millipore). 500 µl of the recombinant SIRT5 elution fractionation was added to the ultra-0.5 centrifugal filter units and centrifuged at 14,000 x g for 15 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... directed against N-terminal epitopes and rabbit p-Ser491 Dab1 antibody (AB9642) were from Merck Chemicals (Darmstadt ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein-G fast flow bead resin and Immobilon-P PVDF membrane were purchased from Merck-Millipore (USA) ...
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bioRxiv - Pathology 2019Quote: ... the gels were blotted to an Immobilon-P PVDF Membrane (Merck Millipore, Burlington, MA, US) using Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2022Quote: ... proteins separated by SDS-PAGE were transferred to a PVDF membrane (Immobilon-P, Merck Millipore) in a Mini Trans-Blot (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... prior to transfer to a PDVF membrane (Immobilon-P Membrane, Merck Millipore Ltd., Burlington, MA). The membrane was then incubated in a 5% milk tris-buffered saline with 0.1% tween-20 (TBS-T ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were transferred into methanol pre-activated polyvinylidene fluoride (PVDF) membranes (Immobilon-P, Merck-Millipore). After transference ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: A549 (human epithelial lung cell line) and EA.hy926 (human endothelial somatic cell line) cells were bought from Merck company and ATCC ...
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bioRxiv - Molecular Biology 2022Quote: CobB driven deacetylation of His-PrsAc was analyzed by Western blot with anti-acetyl lysine antibodies (Sigma/Merck) and mass spectrometry ...
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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Oxidative and non-oxidative active turnover of genomic methylcytosine in distinct pluripotent statesbioRxiv - Cell Biology 2020Quote: ... 10 μg/ml human holo-Transferrin (Merck), 12.5 mg/ml AlbuMAX I (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... and human plasma full-length fibronectin (Merck) was added to the dish at a final concentration of 10 µg/µl for a 1h incubation at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... human recombinant insulin (100 µg/mL; Merck), recombinant human insulin-like growth factor 1 (hIGF1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant human TNFα (GF023) was from Merck. Purified anti-His tag mouse antibody was from BioLegend (USA) ...
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bioRxiv - Cell Biology 2021Quote: ... and human plasma full-length fibronectin (Merck) was added to the dish at a final concentration of 10 μg/μl for a 1h incubation at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... mouse anti-human α5 IgG1 (JBS5; Merck), mouse anti-human α5 IgG3 (P1D6 ...
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bioRxiv - Physiology 2022Quote: ... labelled human mitochondria primary antibody (Merck, MAB1273), 1:500 for 48 h at 4°C and washed with PBS overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... rat anti-human β1 IgG1 (AIIB2; Merck), mouse anti-human β1 IgG1 (Lia1/2 ...
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bioRxiv - Microbiology 2023Quote: ... mouse anti-human α5 IgG3 (P1D6; Merck), mouse IgG1 (JC1 ...
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: ... 180⍰µg/ml Human transferrin (Merck, T8158), 20% D4T Conditioned Media ...
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bioRxiv - Immunology 2023Quote: ... polyclonal anti-human PI3K p85 (Merck Millipore), anti-PI3 Kinase p110α (C73F8 ...
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bioRxiv - Cell Biology 2024Quote: ... or 5µg/cm2 human-plasma fibronectin (Merck) overnight in 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... and the amount of ALP measured using SigmaFast™ p-Nitrophenyl phosphate (pNPP) tablets (Merck KGaA). A standard curve drawn with serial dilution of pNPP and a known quantity of ALP from bovine intestinal mucosa (Merck KGaA) ...
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bioRxiv - Biophysics 2019Quote: ... Protein was transferred overnight at 10 V to an Immobilon-P membrane (Merck Millipore; Burlington, MA) in 25 mM Tris ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Proteins were separated by SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Immobilon-P, Merck Millipore). Proteins of interest were detected using the following primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to a PVDF membrane (0.45µm Immobilon®-P, Merck Millipore Ltd., VIC, Australia) at 100 V in chilled transfer buffer containing 25 mM Tris ...
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bioRxiv - Plant Biology 2022Quote: ... The separated proteins were transferred to polyvinylidene difluoride membranes (Immobilon-P; Merck Millipore, Burlington, MA, USA) and analyzed with the following antibodies ...
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bioRxiv - Plant Biology 2023Quote: ... and proteins were blotted onto a 0.45 µm PDVF Immobilon-P membrane (Merck Millipore, ref. IPVH00010) after electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto methanol pre-activated polyvinylidene fluoride (PVDF) membranes (IPFL00010, Immobilon-P; Merck-Millipore). Membranes transferred from TGX Stain-Free™ gels were imaged and quantified for posterior normalization with a ChemiDoc XRS+ (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: Western blot analysis was carried out by semidry electroblotting on immobilon-P transfer membrane from Merck KGaA (Darmstadt ...
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bioRxiv - Cell Biology 2024Quote: ... and transferred onto methanol-pretreated Immobilon®-P PVDF Membrane (0.45 μm pore size) (Merck; IPVH00010) using BOLT gel transfer cassettes and BOLT transfer buffer (Invitrogen™ ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... rh-HI was prepared in either 20% acetic acid (v/v from acetic acid glacial, Merck KGaA, Darmstadt, Germany), 0.3 M NaCl (Merck KGaA ...
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (10,000 x g, 30 min, RT) and incubated for 1 hour with Ni-NTA His bind resin (Merck) rolling at RT ...