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Citations for Merck :
1 - 50 of 488 citations for P selectin Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... HEK 293 cells were seeded in culture plates (Corning, Merck, Darmstadt, Germany) and incubated in DMEM (PAN Biotech ...
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bioRxiv - Neuroscience 2021Quote: HEK 293 cells were seeded in cell culture plates (Corning, Merck, Darmstadt, Germany) in the day before at the appropriate density ...
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bioRxiv - Genomics 2020Quote: ... was incubated with/without 0.4μg human 6X-His-CDK9/Cyclin T1 (Merck, 140685) and/or 0.4μg human GST-tagged PP2A-α (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... or anti-His (Merck) for His-G5845–1508) ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Immunology 2023Quote: ... using the 293-free transfection reagent (Merck, Darmstadt, Germany) according to the manufactures’ protocols ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: 293 cells were seeded in culture plates (Corning, Merck, Darmstadt, Germany) and incubated overnight at 37°C with 5% CO2 in DMEM (PAN Biotech ...
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bioRxiv - Immunology 2019Quote: ... Immobilon-P® (Merck Millipore). Membranes were incubated with anti-IκBα (9242) ...
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bioRxiv - Immunology 2022Quote: ... Immobilon-P® (Merck Millipore). Membranes were incubated with anti-IκBα (product # ...
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bioRxiv - Immunology 2019Quote: ... Immobilon-P® (Merck Millipore). Membranes were incubated with anti-IκBα (9242) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% HI-FBS (Merck/Biochrom), 2 mM L-glutamine (Merck/Biochrom) ...
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bioRxiv - Systems Biology 2023Quote: ... Primary his tag antibody (05-949, Merck), stored in a skim milk solution ...
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bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
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bioRxiv - Biochemistry 2022Quote: ... and transferred to Immobilon-P (Merck) using HorizeBLOT (ATTO) ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Biochemistry 2024Quote: ... we utilized the His-Bind purification kit (Merck, USA) followed by manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... and 1% penicillin/streptomycin (P/S, Merck). MLE-12 cells were cultured until 90% confluence in tissue culture treated T75 flasks and then trypsinized ...
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bioRxiv - Neuroscience 2020Quote: ... 1% penicillin/streptomycin (P/S, Merck Millipore) and 0.055 mM β l (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... 1% penicillin/streptomycin (P/S, Merck Millipore), 1% non-essential amino acids (Gibco Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... and transferred to Immobilon-P (Merck Millipore) membranes ...
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bioRxiv - Microbiology 2020Quote: ... HEK 293T cells grown on 15-cm dishes were transfected using polyethylenimine (PEI) (Merck), a transfection reagent for standard triple transfection protocol ...
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bioRxiv - Cell Biology 2020Quote: ... then electroblotted onto Immobilon-P membranes (Merck Millipore). Following blocking in 5% dried skimmed milk (Marvel ...
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bioRxiv - Immunology 2021Quote: ... transferred onto PVDF membrane (Hybond P, Merck Millipore), blocked in 5% BSA for 30 min and incubated ON with mouse anti-GAD67 monoclonal antibody (1:6000 ...
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bioRxiv - Immunology 2023Quote: ... and 1X Penicillin-Streptomycin (P/S) (Merck Millipore) (AdDF+++) ...
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was added to a His Trap HP column (Merck) previously equilibrated with buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... previously coupled to 1 ml HI-TRAP NHS columns (GE/Merck) packed with Sepharose pre-activated for covalent coupling of ligands containing primary amino groups ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Merck). Immortalized human C20 microglia were kindly provided by David Alvarez-Carbonell (21) ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Developmental Biology 2020Quote: ... and transferred to Immobilon P membranes (Merck group, IVPD00010) using a semi-dry blotting system (Biometra/ Analytik Jena ...
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bioRxiv - Neuroscience 2021Quote: ... A PVDF membrane (Hybond-P PVDF membranes, Merck, Germany) was immersed in 100% methanol (Nacalai Tesque ...
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bioRxiv - Systems Biology 2021Quote: ... and 1% penicillin/streptomycin (p/s, Sigma-Aldrich/Merck). For differentiation ...
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bioRxiv - Cell Biology 2022Quote: ... Separated proteins were transferred onto Immobilon-P membrane (Merck) using Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (Bio-Rad Laboratories) ...
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bioRxiv - Genetics 2022Quote: ... and transferred onto Immobilon-P PVDF membranes (Merck Millipore). Membranes were blocked with 5% milk in Tris-buffered saline with tween (TBST ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% v/v penicillin/streptomycin (P/S, Merck-SIGMA), 20 ng/ml basic fibroblast growth factor (bFGF ...
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bioRxiv - Cancer Biology 2020Quote: ... and transferred onto Immobilon-P PVDF membranes (Merck Millipore). Membranes were blocked with 5% milk in Tris-buffered saline with tween (TBST ...
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bioRxiv - Molecular Biology 2020Quote: ... and transferred onto Immobilon-P PVDF membranes (Merck Millipore). Membranes were incubated with 5% skimmed milk in PBS-T (PBS (Amresco ...
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bioRxiv - Cancer Biology 2021Quote: ... the chromatography utilized a ZIC-p(HILIC) column (Merck SeQuant ZIC-pHILIC 5um 150 x 4.6 mm ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were electroblotted onto Immobilon-P transfer membranes (Merck). The membranes were blocked with Blocking One for 30 min at room temperature and then incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Genetics 2022Quote: ... and transferred onto Immobilon-P PVDF membranes (Merck Millipore). Membranes were blocked with 5% milk in Tris-buffered saline with tween (TBST ...
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bioRxiv - Cell Biology 2022Quote: ... erk-p (clone AW39R, 1/500, Merck Millipore, USA), p75 antibody (1/1000 ...
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bioRxiv - Genomics 2022Quote: ... and transferred to immobilon-P transfer membranes (Merck Millipore). Immunodetection was carried out with anti-phospho-STAT3 (Tyr705 ...
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bioRxiv - Cancer Biology 2023Quote: ... and transferred onto Immobilon-P PVDF membranes (Merck Millipore). Membranes were blocked with 5% milk in Tris-buffered saline with tween (TBST ...
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bioRxiv - Genetics 2023Quote: ... and Collagenase Type P (0.4 mg/mL, Merck Millipore) in Leibovitz’s L-15 Medium (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfer to Immobilon-P 0.45μm PVDF membrane (Merck Millipore) was carried out in NuPAGE Transfer buffer (NP00061 ...
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bioRxiv - Plant Biology 2023Quote: ... Gels were bloted to PVDF membrane (Immobilon-P, Merck) that were then incubated for 1 hr with a PLC2-antibody (1:2000 ...
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bioRxiv - Cell Biology 2023Quote: ... and transferred to Immobilon-P PVDF Membrane (Merck Millipore) for detection ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto Immobilon-P PVDF membranes (Merck) using a semi-dry blotting system (ATTO) ...
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bioRxiv - Neuroscience 2023Quote: ... rat anti-Substance P (Merck & Co., Inc.; 1:500), rabbit anti-Substance P (ImmunoStar Inc. ...