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Citations for Merck :
101 - 145 of 145 citations for Legionella pneumophila antigen native extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Biodiversity and Antimicrobial Potential of Bacterial Endophytes from Halophyte Salicornia brachiatabioRxiv - Microbiology 2020Quote: Purification of compounds was carried out by separating 50 mg of crude extract on preparative TLC plates (Kieselgel 60 F254, 25 mm, Merck) using the same solvent system as above ...
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bioRxiv - Molecular Biology 2020Quote: ... Lipid extracts corresponding to 1 mg wet liver weight were applied onto HPTLC silica gel 60 plates (10 × 20 cm: Merck) and plates were developed in n-hexane/diethylether/acetic acid 70:30:5 (v/v/v ...
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bioRxiv - Microbiology 2021Quote: ... The sonicated cellular extract was spun down and the supernatant has been incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... appropriate amounts of polar lipid extracts were spotted onto silica gel high-performance thin-layer chromatography (HPTLC) plates (silica gel 60 F254, Merck) using a Linomat 5 sample application unit (CAMAG ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mL of filtered protein extract was mixed with 40μL of anti-FLAG M2 magnetic beads (Merck, formerly Sigma-Aldrich) (equilibrated in GTEN + 0.1 % Tween-20 prior to use ...
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bioRxiv - Plant Biology 2023Quote: ... Cell debris was removed with centrifugation and 2+2 μl of extracts and a commercial pinosylvin standard (ArboNova) were applied on TLC plates Silica gel 60 F254 (Merck), which were developed with chloroform ...
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bioRxiv - Microbiology 2022Quote: ... Purification of compound 9 was carried out through column chromatography by passing the concentrated crude extract through a packed silica gel 60 (Merck) in a column ...
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bioRxiv - Physiology 2023Quote: ... and 20 µg protein extracts were separated with 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and slit into two parts ...
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bioRxiv - Microbiology 2023Quote: ... Lipid extracts were then applied to silica gel high-performance thin-layer chromatography (HPTLC) plates (silica gel 60 F254, Merck) using a Linomat 5 sample application unit (CAMAG) ...
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bioRxiv - Cell Biology 2023Quote: For the thin layer chromatography (TCL) an aliquot of lipid extract corresponding to 8 mg of DCW was applied to silica gel TLC plates (Merck) by a Linomat 5 semiautomatic sample applicator (Camag) ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting pellet was retained and subject to extraction following the RED Extract Plant PCR-Kit (Merck KGaA, Darmstadt, Germany) protocol.
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bioRxiv - Microbiology 2020Quote: A 5-10 µl aliquot of the filtered Aqueous Garlic Extract (AGE) was applied 1 cm away from the base of silica gel 60 TLC plate (Merck Millipore). The applied sample was air dried for 10 min at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ml of extract was incubated overnight at 4°C with rotation with 20 µg/ml anti-FLAG M2 antibody (Merck, F3165) and 50 µl Pierce magnetic protein A/G beads (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... 10 μL of the extract-reaction mixture was added to 1 cm2 P81 Phosphocellulose paper squares (MERCK MILLIPORE, Billerica, MA, USA). The papers were immersed directly in 1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... crescentus cultures were grown overnight at 30 °C in 3 ml of 2x PYE49 (Peptone, Merck, #82303; Yeast Extract, Merck, #Y1626) medium under mechanical agitation (200 rpm) ...
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bioRxiv - Cell Biology 2022Quote: ... crescentus cultures were grown overnight at 30 °C in 3 ml of 2x PYE49 (Peptone, Merck, #82303; Yeast Extract, Merck, #Y1626) medium under mechanical agitation (200 rpm) ...
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bioRxiv - Microbiology 2022Quote: ... cultures were maintained at 30 °C in MGY M9 medium (10 g/L glycerol, 1.25 g/L yeast extract, M9 salts) or Standard I Nutrient Agar (Merck, Darmstadt, Germany) supplemented with 1% glycerol ...
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bioRxiv - Plant Biology 2022Quote: CGMMV vsiRNAs were quantified by RT-qPCR according to Shi and Chiang (2005) with some modifications: 2 μg of RNA extracts from the cucumber leaves were treated with DNaseI (Merck, Spain) and polyadenylated using the Poly(A ...
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bioRxiv - Synthetic Biology 2020Quote: ... Growth experiments were performed on YNB medium with 1 or 2 % methanol supplemented with 1 g/L yeast extract (Merck 103753). Optical density readings at 600 nm (OD600 ...
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bioRxiv - Genomics 2019Quote: ... ChIP was performed on this chromatin extract using 1 µL of Anti-acetyl-Histone H4 (Lys16) Antibody (Merck Milipore, 07-329) or 0.5 µL of Histone H3 antibody mAb MABI 0301 (Active Motif ...
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bioRxiv - Cell Biology 2019Quote: ... cleared extracts were incubated ON at +4 °C in an orbital shaker with anti-FLAG sepharose beads (M2 clone, Merck, A2220). Bound complexes were pelleted ...
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bioRxiv - Cell Biology 2019Quote: ... cleared extracts were incubated 2h-6h at +4°C in an orbital shaker with anti-FLAG sepharose beads (M2 clone, Merck, A2220). Bound complexes were pelleted and 5x washed (Lysis buffer with 1M NaCl) ...
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bioRxiv - Microbiology 2020Quote: ... rhizoxinica were grown at 30 °C in MGY M9 medium (10 g/L glycerol, 1.25 g/L yeast extract, M9 salts) or Standard I Nutrient Agar (Merck, Darmstadt, Germany) supplemented with 1% glycerol.
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bioRxiv - Microbiology 2020Quote: The crude extract and purified fractions were separated on TLC Silica gel 60 F254 plates (Merck Life Science Pvt. Ltd., Germany) using the solvent system of hexane ...
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bioRxiv - Biochemistry 2021Quote: Separation of phospholipids by 2D-TLC Total lipid extracts corresponding to 200 nmol phospholipid were applied on silicagel plates (Merck 1.05641), freshly impregnated with 2.4% (w/v ...
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bioRxiv - Synthetic Biology 2021Quote: ... 500 μL of the perchlorate extract was filtered with a 30K centrifugal filter (Amicon Ultra - 0.5mL, Ultracel - 30K, UFC503096, Merck KGaA, Germany), at 14000 g ...
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bioRxiv - Microbiology 2019Quote: ... Metabolite separation was performed by injecting 7 µL of the extract into a SeQuant® ZIC-pHILIC PEEK coated column (150 mm × 4.6 mm, 5 µm polymer, Merck Millipore) maintained at 25°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... cerevisiae strains for preparation of competent cells were cultivated in YPD medium consisting of 10 g/L yeast extract (Merck Millipore), 20 g/L peptone (Difco ...
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bioRxiv - Molecular Biology 2022Quote: ... yeast of cell line BY4741 (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0) was grown in 1 litre of YPD (yeast extract (Merck/Sigma-Aldrich cat. no. 70161), peptone (Merck/Sigma-Aldrich cat ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts of equal total protein (20 μg each) were separated by SDS-PAGE and transferred to Nylon membrane (Merck Millipore). The membranes were blocked with 5% nonfat milk in TBST washing buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The routinely used growth medium was composed of yeast extract (1%(w/v)) (Fisher BioReagents™) and casein peptone (2%(w/v)) (Merck), and was supplemented with 2% (w/v ...
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bioRxiv - Immunology 2023Quote: ... The digested suspension was pelleted and the pepM extract buffer exchanged to PBS pH 7 using 10 kDa Amicon centrifugal filter unit (Merck, Ireland). PepM extracts were confirmed with SDS-PAGE and stored in solution at − 20°C.
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bioRxiv - Plant Biology 2024Quote: ... Pto DC3000 ΔavrPto ΔavrPtoB was cultured in NYG-medium (0.5% [w/v] peptone, 0.3% [w/v] yeast extract, 2% [v/v] glycerol; Merck KGaA, Darmstadt, Germany) with appropriate antibiotics (50 µg/ml rifampicin ...
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bioRxiv - Immunology 2022Quote: Reduced cell extracts (20 µg of protein) or immunoprecipitates were resolved by SDS-PAGE and transferred to PVDF membranes (Merck-Millipore, #10344661) using a Trans-Blot transfer cell (BioRad) ...
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bioRxiv - Zoology 2023Quote: ... about 10 mL of CLE flower extract was added to a 90 mL aqueous solution of 1 mM AgNO3 (Merck Laboratories, India) and kept at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein from tissue extracts or plasma were prepared in Laemmli or RIPA buffers (both from Merck Life Science UK Ltd, UK), separated by polyacrylamide gel electrophoresis and then transferred to either nitrocellulose or a polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2023Quote: Quantification of polar lipids content on HPTLC was carried out as follows: Lipid extracts and polar lipids standards at varying concentrations were applied onto silica gel 60 F254 HPTLC plates (Merck KGaA, Germany) using an ATS 5 automatic TLC sampler (Camag ...
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bioRxiv - Microbiology 2020Quote: ... Glycolipid extracts of up to 15 μl were applied evenly onto half TLC silica plates (20 x 10 cm, Merck KGaA, Darmstadt, Germany). After drying ...
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bioRxiv - Microbiology 2019Quote: ... 25 µl of the extract was spotted (2 µl/transfer) onto a reverse-phase thin layer chromatography silica gel 60 RP-18 sheet (Merck, Kenilworth, NJ, USA). In addition to the LC510 AHL extract ...
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bioRxiv - Plant Biology 2021Quote: ... The dried extracts were dissolved in 350 μl purified water and filtered through MultiScreen PCR-96 Filter Plate membranes (Merck Millipore, Darmstadt, Germany) to remove high-molecular-mass compounds ...
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bioRxiv - Biophysics 2019Quote: ... reduced and alkylated protein extracts were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, # MRCPRT010 Ultracel YM-10). Digested samples were desalted using ZipTips according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 15 cm dishes were seeded and the nuclear translocation of SAMD1 was induced 24 h before extract preparation by adding 200 nM 4-OHT (Merck; 68392-35-8). After collection ...
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bioRxiv - Genetics 2023Quote: ... The molecular sieves were filtered out by loading the extract into a glass funnel (50 mm inner diameter) with glass wool (Merck, KGaA, Darmstadt, Germany) and 0.2 g silica gel (High purity grade ...
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bioRxiv - Plant Biology 2022Quote: Lipid extracts from 500 mg mitochondria and 50 mg leaves were spotted onto TLC 60 plates (20 × 20 cm2, Merck KGaA, Darmstadt, Germany) in parallel with the following standards ...
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bioRxiv - Microbiology 2021Quote: ... Bacilli were grown overnight in Lysogeny Broth ([Difco]; Bacto tryptone 10 g/l, [Oxoid]; yeast extract 5 g/l, [Merck]; NaCl 10 g/l, pH value 7.2 ± 0.2) at 37°C with agitation at 250 rpm.