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Citations for Merck :
1 - 50 of 145 citations for Legionella pneumophila antigen native extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The Legionella pneumophila DrrA 8-533aa (referred to as DrrA)-encoding DNA was cloned into a modified pET19 vector (Merck Millipore, Burlington, Massachusetts) as previously described (Müller et al. ...
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bioRxiv - Immunology 2021Quote: Native human insulin (Merck) was pre-diluted in water to 1 mg/mL ...
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bioRxiv - Microbiology 2023Quote: Purified proteins and native protein standard (Supelco-Merck) were loaded in 4-15% gradient mini protean precast native gel (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... yeast extract (Merck) 2 ...
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bioRxiv - Developmental Biology 2022Quote: ... Antigen retrieval in citrate buffer (Merck) was followed by permeabilization with Triton X-100 (Merck ...
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bioRxiv - Microbiology 2019Quote: ... Malt Extract Agar (MEA, Merck), Corn Meal Agar (Oxoid) ...
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bioRxiv - Cancer Biology 2019Quote: ... mouse anti-human nuclear antigen (1:100; Merck), Alexa Fluor 647-conjugated rat anti-CD45 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... recombinant hepatitis B surface antigen (HBsAg) manufactured by Merck & Co. ...
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bioRxiv - Pathology 2022Quote: ... XO Activity was expressed as units/mg protein using native XO from buttermilk (Merck Millipore) as standard.
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bioRxiv - Microbiology 2020Quote: ... mouse anti-flavivirus group antigen (1:800, MAB10216, Merck Millipore) and goat anti-Iba1 (1:300 ...
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bioRxiv - Biochemistry 2022Quote: ... Cell lysis and membrane protein extraction was performed using the ProteoExtract Native Membrane kit (Merck Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... SV40 T Antigen (Ab-2) (Merck Millipore; DP02; 5 μg/ml). Secondary antibodies (all from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated with 2.5 µg/mL soluble HER2 antigen (Merck) and then with 1:250 APC-labeled anti-human HER2 antibody (Biolegend) ...
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bioRxiv - Bioengineering 2022Quote: ... Antigen retrieval was carried out with pronase (3.5 U/ml; Merck) at 37 °C for 25 minutes ...
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bioRxiv - Synthetic Biology 2021Quote: ... HER2 binding was confirmed with 2.5 µg/mL soluble HER2 antigen (Merck) and subsequent 1:200 APC-labeled anti-HER2 antibody (Biolegend ...
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bioRxiv - Microbiology 2020Quote: ... a commercial mouse anti-flavivirus group antigen monoclonal antibody (MAB10216; Merck Millipore) diluted 1:1,000 in phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... Cell extract was treated with 1 µL Benzonase (Merck), supplemented with 0.15% (v/v ...
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bioRxiv - Genomics 2020Quote: ... ChIP was performed on native material (without crosslinking) using antibodies targeting histone modifications H3K4me2 (Merck ref. 07-030), H3K9me3 (Active Motif ...
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bioRxiv - Neuroscience 2024Quote: ... Surface antigens were labeled with primary antibody (rabbit anti-GFP, 1:1000, Merck) for 15 min on ice ...
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bioRxiv - Plant Biology 2021Quote: ... brassicicola was maintained on malt medium (3% malt extract (Merck), 1.5% agar-agar (Roth) ...
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bioRxiv - Plant Biology 2021Quote: ... The protein extracts were then filtered through Miracloth (Merck, Germany) and subjected to a Co-IP analysis.
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bioRxiv - Plant Biology 2023Quote: ... Protein extracts were supplemented with 0.5 mM cycloheximide (CHX; Merck), and the mixtures were then divided in two parts ...
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bioRxiv - Biochemistry 2021Quote: ... incubated with ReBlot Plus strong antigen retrieval agent (Merck, Watford, UK, cat. no. 2504) for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... and NG2 (neuron-glial antigen 2, AB5320, Merck Millipore, Burlington, MA, USA, 1:400) were used.
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bioRxiv - Cell Biology 2021Quote: ... The extracts were digested with 800 U of benzonase (Merck, E1014) per mL of extract for 30 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... the dried extract was dissolved in ethyl acetate (EtOAc) (Merck, 1.06923.2511) to a final concentration 0.1 mg/μl (of pellet wet weight) ...
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bioRxiv - Cell Biology 2023Quote: ... extracts were transferred to polyvinylidene fluoride (PVDF) membranes (Merck Millipore, German). Membranes were blocked with 5% defatted milk and then incubated with primary antibodies against anti-DNMT1 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 1x Protease Inhibitor Cocktail for plant cell and tissue extracts (Merck), 2% n-Dodecyl-beta-Maltoside Detergent (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... protein extracts were purified with 10 kDa Amicon filters (Merck, Darmstadt, Germany). Protein concentration was determined with Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... crescentus cells on the PYE (peptone, Merck, #82303; yeast extract, Merck, #Y1626) agar plate was inoculated into 3 ml of M2G medium to grow overnight at 28 °C under mechanical agitation (200 r.p.m.)35 ...
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bioRxiv - Microbiology 2023Quote: ... crescentus cells on the PYE (peptone, Merck, #82303; yeast extract, Merck, #Y1626) agar plate was inoculated into 3 ml of M2G medium to grow overnight at 28 °C under mechanical agitation (200 r.p.m.)35 ...
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bioRxiv - Cell Biology 2020Quote: ... Antigen retrieval was carried out with 0.2 U/mL Chondroitinase ABC (C2905, Sigma, Merck, Darmstadt, Germany) at 37 °C for two hours for DCN ...
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bioRxiv - Microbiology 2020Quote: ... The released antigen was then concentrated with the Amicon 3 kDa filter (Merck KGaA, Darmstadt, Germany) before being analysed by SDS gel electrophoresis ...
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bioRxiv - Microbiology 2021Quote: ... The crude extract was filtered through a 0.22 μm membrane filter (Merck Millipore) and loaded on a disposable purification column packed with TALON Superflow Resin (Clontech) ...
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were quantified using Direct Detect® Assay-free Cards (Merck; DDAC00010) or Pierce Bicinchoninic Bcid (BCA ...
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bioRxiv - Microbiology 2019Quote: ... Apoplast extracts were filtered sterilised through a 0.1 μm filter (Durapore, Merck, Germany). Extracts were made from ~ 5 plants per sample for leaves and roots and up to 24 plants for apolastic washings or for sprouts ...
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bioRxiv - Microbiology 2019Quote: ... The following concentrations of hygromycin B added to malt extract agar (MEA; Merck) were tested ...
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bioRxiv - Cancer Biology 2020Quote: ... Methanol extracts were diluted 1:5 in Milli-Q water (Merck, Darmstadt, Germany) and subjected to reversed phase HPLC-MS using an Accela 1250 HPLC system equipped with a Hypersil Gold aQ column and coupled to a QExactiveTM mass spectrometer (all from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Crude protein extract was incubated with Anti-FLAG® M2 Magnetic Beads (Merck) overnight ...
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Phospholipid flippases and Sfk1 are essential for the retention of ergosterol in the plasma membranebioRxiv - Cell Biology 2020Quote: ... lipid extracts containing 20 nmol phosphates were subjected to high-performance TLC (Merck) separation with hexane/diethyl ether/formic acid (40:10:2 ...
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bioRxiv - Biochemistry 2020Quote: ... MEFs were immortalized with SV40 large T-antigen by transfection using GeneJuice transfection reagent (Merck Millipore 70967).
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bioRxiv - Neuroscience 2020Quote: ... Sections were deparaffinized and submitted to antigen retrieval by incubating the slides in sodium citrate (Merck KGaA) 10mM pH 6.0 for 1 h in a 95°C water bath ...
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bioRxiv - Microbiology 2021Quote: ... Equal volumes of lipid extracts were spotted onto silica 60 F254 HPTLC plates (Merck) and migrated with chloroform:methanol:ammonium hydroxide (30 % ...
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bioRxiv - Microbiology 2020Quote: ... Saponin fraction (SAP): 20 g of extracts were exhaustively extracted with butanol (Merck, 95%), monitored by hemolysis-TLC.
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bioRxiv - Cancer Biology 2021Quote: ... An aliquot of extract containing 100 µg protein was then digested by benzonase (Merck) and precipitated by acetone ...
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bioRxiv - Biochemistry 2022Quote: ... Protein extracts were further processed in a Microcon-30 kDa Centrifugal Filter Units (Merck) in a tempered centrifuge at 24°C ...
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bioRxiv - Cancer Biology 2022Quote: ... extracts were incubated with 40 μL equilibrated ANTI-FLAG M2 Affinity Gel (Merck, A2220) for approximately 3 hours at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... 0.5% NaCl (Mercury Cat#1064041) and 0.5% yeast extract (Merck Cas#8013-01-2)) supplemented with 250 mg/ml glucose (Mercury Cas#50-99-7) ...
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bioRxiv - Microbiology 2022Quote: ... The lipid extracts were spotted on to silica-gel coated TLC plates (Merck, USA) and normalized according to the scintillation counts ...
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bioRxiv - Molecular Biology 2023Quote: ... extracts were incubated with 20 μL equilibrated anti-FLAG M2 Affinity Gel (Merck, A2220) for approximately 3 hours at 4 °C ...