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Citations for Merck :
151 - 196 of 196 citations for Kinase Domain of Pyruvate Kinase liver Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... Peptide fractions were eluted using MeOH buffer containing trifluoroacetic acid (TFA) (Merck Sigma, Cat. No. 302031), FA or ABC ...
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bioRxiv - Immunology 2022Quote: ... The proteolytically cleaved peptides were extracted and desalted using ZipTip μC18 tips (Merck Millipore, Darmstadt, Germany). The peptide lysates were re-suspended in 15 µl 0.1% formic acid and analysed by nanoliquid chromatography mass spectrometry (UltiMate 3000 RSLCnano ...
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bioRxiv - Biophysics 2022Quote: ... Both recombinant and synthetic lyophilized peptides were dissolved in 6M guanidine thiocyanate (Merck KGaA, Darmstadt, Germany) and incubated overnight at 1250 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were eluted with 50 μl of 100 μg/mL of 3X FLAG tag peptide (Merck) in solubilization buffer followed by acetone precipitation (overnight ...
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bioRxiv - Cell Biology 2023Quote: ... pH 2.0) for endogenous MDH2 IP and incubation with 250 μg/ml 3XFLAG peptide (Merck F3290) in the case of FLAG IP ...
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miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... Labelled peptides were separated on Chromolith Caprod RP-18e HR capillary column (150 × 0.1 mm; Merck Millipore) using linear gradient of buffer B (98% ACN and 0.05% TFA in water ...
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bioRxiv - Cell Biology 2020Quote: ... and proteins were eluted using 100 µl of 150 ng/µl 3× FLAG Peptide (F4799, Merck KGaA) or HA peptide (HY-P0239 ...
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bioRxiv - Microbiology 2022Quote: ... 100 µg of the dried peptides were dissolved in 90 µl of 0.2 M EPPS buffer (MERCK)/10% acetonitrile ...
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bioRxiv - Pathology 2019Quote: ... The extracted peptides were purified using C18 Zip-TIP pipette tips according to the manufacturer’s instructions (Merck Chemicals ...
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bioRxiv - Biochemistry 2019Quote: Crude peptides were purified by reverse-phase high-performance liquid chromatography using a Chromolith Prep column (Merck) on a Prominence LC-20AP system (Shimadzu ...
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bioRxiv - Biochemistry 2019Quote: Peptides were spotted at 40 µg per origin and dried on thin-layer cellulose plates from Merck. Electrophoresis was run in 1% ammonium carbonate pH 8.9 at constant 1 kV for 25 min 40,41 ...
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bioRxiv - Immunology 2020Quote: ... MHC–peptide complexes were eluted by repeated addition of 0.2% trifluoroacetic acid (TFA, Merck, Whitehouse Station, NJ). Peptides were purified by ultrafiltration using centrifugal filter units (Amicon ...
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bioRxiv - Biochemistry 2023Quote: ... the peptides were desalted and intact glycopeptides were enriched by ZIC-HILIC beads (Merck, Product no. 1.50458.0001) according to our previous study[25].
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bioRxiv - Plant Biology 2021Quote: ... Protein complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: Peptides EcFtsN1-32-C and VmFtsN1-29-C were labelled with maleimide-Atto 495 (Merck Life Science UK). EcFtsA1-405 and VmFtsA1-396 were buffer exchanged into binding buffer (50 mM HEPES/KOH ...
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bioRxiv - Microbiology 2021Quote: Peptides EcFtsN1-32-C and VmFtsN1-29-C were labelled with maleimide-Atto 495 (Merck Life Science UK). EcFtsA1-405 and VmFtsA1-396 were buffer exchanged into binding buffer (50 mM HEPES/KOH ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were dissolved in 2% acetonitrile containing 0.1% trifluoroacetic acid and desalted using C18 ZipTips (Merck Millipore, Germany). Each sample was independently analysed on a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... The peptides were diluted to 50 µg/mL in 10 mM sodium acetate pH 5 (Merck, Darmstadt, Germany) and injected overflow cell two of each channel to a final signal of 3400 RU for L-CDP1 and 1300 RU for L-CDP7 ...
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bioRxiv - Microbiology 2024Quote: ... and subsequently eluted in 150 μL of wash buffer with 500 ng/μL FLAG peptide (Merck, Darmstadt, Germany) by rotation on a rotator for 2 h at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... (final concentration of 2µg/mL) and pools of original or beta variant peptides on hydrophobic-PVDF ELISpot plates (Merck) coated with 5µg/mL anti-mouse IFNγ (AN18) ...
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bioRxiv - Systems Biology 2021Quote: ... Peptides were eluted in a final volume of 600 μL and filtered with 0.22 μm PVDF filters (Merck Millipore) before dried in a centrifugal vacuum concentrator ...
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bioRxiv - Immunology 2020Quote: ... the reduced peptide solution was purified five times using ZipTip Pipette Tips with C18 resin and 0.6 µl bed volume (Merck,) and eluted in 32.5% ACN/0.2% TFA ...
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bioRxiv - Neuroscience 2022Quote: ... was used in a concentration of 20 µM and autocamtide-2 related inhibitory peptide II (AIP-II; Merck Millipore) at 4 µM.
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bioRxiv - Plant Biology 2023Quote: ... TF complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore F4799). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher 88843 ...
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bioRxiv - Cell Biology 2023Quote: ... and were treated with various concentrations of peptides (as determined by prior Hoechst 33258 staining in serum–free media) or solvent (DMSO, 0.1% v/v, Merck) or positive control TGF-β1 10ng/ml (Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... On the next day the peptide and GFP were subject to concentration via ultrafiltration (Amicon Ultra 10 kDa MWCO, Merck) and removal of the GFP and 3C protease ...
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bioRxiv - Microbiology 2019Quote: ... Solid phase synthesis took place on a CF peptide synthesizer (Intavis) using a Rink Amide LL resin (100 µmol; Merck) and N(α)-Fmoc amino acids ...
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bioRxiv - Systems Biology 2020Quote: ... The resultant pellets were resuspended in 3% acetonitrile + 0.1% trifluoroacetic acid and peptide quantification performed using the Direct Detect system (Merck Millipore). Protein samples were normalized then vacuum concentrated in preparation for mass spectrometry.
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bioRxiv - Pathology 2019Quote: ... For MALDI-TOF/TOF/MS analysis dried peptides were dissolved in peptide resuspension solution (0.1% TFA in 5% ACN) and desalted/concentrated using C18 zip tips (Merck Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... The mix was transferred to a plate coated with avidin and the FLAG-peptide was detected with an anti-FLAG-alkaline phosphatase conjugate (Merck), developed with pNPP SigmaFAST (Merck ...
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bioRxiv - Immunology 2023Quote: 200 μg of HLA.A2-peptide complex in PBS was concentrated to 1 mg ml−1 with the use of Amicon Ultra Centrifugal Filters (Merck), adjusted to pH 8.3 by adding freshly prepared NaHCO3 (0.1 M final concentration ...
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bioRxiv - Genetics 2023Quote: ... membranes were incubated with an anti-sera peptide based polyclonal rabbit GLK2-antibody (1:3,000) and a peroxidase-conjugated anti-rabbit antibody (1:10,000) (Merck, Darmstadt, Germany) for 1 h at room temperature in TBST [Tris-HCl 20 mM (pH 7.6) ...
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bioRxiv - Microbiology 2019Quote: ... Solid phase synthesis took place on a CF peptide synthesizer (Intavis) using a Fmoc-PEG-Biotin NovaTag ™ resin (100 µmol; Merck), 2-Azidoacetic acid (Fluorochem ...
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bioRxiv - Immunology 2020Quote: ... Peptides were separated from the HLA molecule remnants by ultrafiltration employing 3 kDa and 10 kDa Amicon filter units (Merck Millipore) for HLA-I and HLA-II ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then resuspended in 10 μl of 0.1% trifluoroacetic acid and tryptic peptides were desalted using a Zip-Tip® column (Merck Millipore) pipette tip according to the manufacturer’s protocol and dried in a vacuum concentrator before analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
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bioRxiv - Biochemistry 2023Quote: All peptides used in this study probes were designed as 11-mers bearing biotinylated PEG2 linker in the N terminus and an amide group in the C terminus and were synthesized using Fmoc-solid-phase peptide chemistry with Novasyn TGR resin (Merck Millipore) as described in the Novabiochem Innovations 01/11 ...
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bioRxiv - Systems Biology 2024Quote: ... the acidified peptide solution was cleaned-up on self-packed StageTips (70) accommodating four discs of Empore C18 material (Merck Supelco). The material was prepared by consecutive application of pure ACN (Biosolve Chimie) ...
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bioRxiv - Cancer Biology 2024Quote: ... a cassette coding for tdTomato with an N-terminal nuclear localization sequence and lack of a stop codon and a puromycin cassette with an N-terminal self-cleaving T2A peptide were amplified by KOD polymerase (Merck, 71085) and subjected to Gibson assembly.
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bioRxiv - Neuroscience 2020Quote: ... glial glutamate transporter GLT1 (guinea pig, polyclonal, synthetic peptide from the C-terminus of rat GLT1, Merck, AB1783, AB_90949, dilution 1:1,000), GFP (chicken ...
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bioRxiv - Plant Biology 2021Quote: ... 1000 μl tips were fitted with 2 plugs of C18 octadecyl 47mm Disks 2215 (Empore™) material and 10 μg of LiChroprep® RP-18 peptides (Merck). Tips were sequentially equilibrated with 100 % methanol ...
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bioRxiv - Plant Biology 2021Quote: ... Extracted peptides were dried in a speed-vac and resuspended in (v/v) 2% acetonitrile/0.2% trifluoroacetic acid (Merck, catalog no. 302031). A total of four biological replicates for each sample type was submitted.
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bioRxiv - Plant Biology 2023Quote: ... Pulldowns were done as described17 with the following modifications: The biotinylated Aux/IAA7 DII peptide (AKAQVVGWPPVRNYRKN, N-terminal biotin) was bound to streptavidin-agarose beads (Merck, #16-126). 250 μl of total protein extract was combined with 45 μl of DII-beads and either 0 μM or 10 μM cvxIAA or 10 μM IAA ...
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bioRxiv - Plant Biology 2020Quote: ... the ORF of CLEL6 lacking the predicted signal peptide was amplified by PCR from a previous construct and cloned into the NcoI restriction site of pETDuet1 (Novagen/Merck KGaA, Darmstadt, Germany). Correct orientation and translational fusion with the C–terminal His-tag was verified by sequencing ...
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bioRxiv - Plant Biology 2020Quote: ... ORFs of PSK1 and RGF1 lacking the predicted signal peptides were amplified by PCR from cDNA and cloned into the NcoI restriction site of pETDuet1 (Novagen/Merck KGaA, Darmstadt, Germany). C-terminal His tags were added by including 6 His codons in the reverse PCR primers ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Immunology 2020Quote: Following protein digestion a 5% volume of the peptide solution was cleaned up with a reverse phase (C18) ZipTip (Merck Millipore, Burlington, MA, USA). The peptide sample was acidified with the addition of 1 μl 1M hydrochloric acid ...