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Citations for Merck :
101 - 150 of 196 citations for Kinase Domain of Pyruvate Kinase liver Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... the peptides were dried and extracted using C18 Zip-tip (Merck Millipore).
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bioRxiv - Immunology 2022Quote: ... the peptides were dried and extracted using C18 Zip-tip (Merck Milipore). The peptides were dried again ...
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bioRxiv - Genetics 2019Quote: Eluted monomethyl peptides were concentrated and purified using a Ziptip (Merck-Millipore). The Ziptip was first equilibrated in 50 μL of 50% acetronitrile ...
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bioRxiv - Synthetic Biology 2020Quote: ... The digested peptide mixtures were concentrated using Millipore® ZipTip C18 (Merck) eluting with 70 % acetonitrile ...
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bioRxiv - Immunology 2020Quote: ... Peptides were isolated using reversed-phase HPLC (Chromolith C18 Speed Rod, Merck) on an Akta Ettan HPLC system (GE HealthCare) ...
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Systematic Analysis of Lysine Succinylation in Vero cells infected with Small Ruminant MorbillivirusbioRxiv - Genomics 2021Quote: ... The resulting peptides were desalted with C18 ZipTips (Merck Millipore, Billerica, USA) according to the manufacturer’s instructions and dried by vacuum centrifugation.
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bioRxiv - Biochemistry 2021Quote: The digested peptides were cleaned up using ZipTip C18 (Merck Millipore, Ireland), dried down ...
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bioRxiv - Biophysics 2021Quote: ... Peptide samples were initially pre-treated with 1,1,1,3,3,3-hexafluoro-isopropanol (HFIP) (Merck), then dissolved in traces of dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2022Quote: ... the peptides were cleaned up using Ziptips with 0.6 µl C18resin (Merck). The samples were injected to an Orbitrap Fusion Lumos ...
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bioRxiv - Neuroscience 2023Quote: ... the peptides were purified and concentrated using a C18 ZipTip (Merck Millipore) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... The peptides were dried and extracted using C18 Zip-tip (Merck Milipore). The peptides were dried again ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were concentrated and eluted using C18 ZipTips (Merck Millipore, Darmstadt, Germany) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptide alkylation was done with 10 mM final Iodoacetamide (Merck; cat. A3221) at RT in the dark for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The peptides were cleaned using C18-reverse-phase ZipTipTM (Millipore, Merck KGaA). The dried peptide digest was re-suspended in 1% trifluoroacetic acid and sonicated in a water bath for 1 min before injection ...
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bioRxiv - Microbiology 2024Quote: ... peptides were cleaned using in-house prepared C18 tips (66883-U-Merck).
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bioRxiv - Pathology 2019Quote: ... the membranes were probed with 1 μg/mL mAb 9G3 against the glycosylated epitope ‘KEPAPTTT’ in the lubricin mucin domain (Merck KGaA, Darmstadt, Germany 51) or polyclonal anti-CG antibody (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Organic acids (lactate, acetate, pyruvate and succinate) were analyzed using a reversed-phased HPLC (Merck-Hitachi, Darmstadt, Germany) equipped with a diode array detector (L-7450 ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting peptides were desalted with C18 ZipTip pipette tips (Merck Millipore, ZTC18S960) and loaded onto an Ultimate 3000 UPLC system (Dionex ...
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bioRxiv - Microbiology 2022Quote: ... Peptide content in each fraction was verified using QuantiPro BCA Assay Kit (Merck). Zn content was determined using PAR against a standard curve.
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bioRxiv - Developmental Biology 2022Quote: ... subsequently the peptides were eluted by 95% (v/v) acetonitrile (Merck, Darmstadt, Germany), 0.1% (v/v ...
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bioRxiv - Neuroscience 2019Quote: ... The peptides were acidified to a total concentration of 1% Formic Acid (Merck). Samples were cleaned up using OASIS sample cleanup cartridges (Waters ...
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bioRxiv - Synthetic Biology 2020Quote: ... the peptide samples were filtered through 0.45 μm filters (Merck KGaA, Darmstadt, Germany). For the digestion of rplF ...
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bioRxiv - Immunology 2021Quote: ... Proteins were eluted from beads using 40 µg of 3x FLAG peptide (Merck) for 200µL of IP sample incubated on a rotator for 45 min at 4°C.
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bioRxiv - Developmental Biology 2023Quote: ... subsequently the peptides were eluted by 95% (v/v) acetonitrile (Merck, Darmstadt, Germany), 0.1% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... and the immunopurified complexes were eluted with 3X FLAG peptide (Merck Millipore/Sigma). For tandem IP experiment ...
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bioRxiv - Cell Biology 2023Quote: ... and the excess peptide was depleted via Amicon Ultra Centrifugal Filters (Merck Millipore). To label the azide-coupled nanobody with a fluorophore ...
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bioRxiv - Microbiology 2021Quote: ... One mL of virus stock and 9 mL of MEM-E supplemented with 1% pyruvate (Merck KGaA, Darmstadt, Germany) were added to a conical tube with 16 × 106 SK-6 cells and shaken for 30 minutes at 104 rpm and 37°C ...
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bioRxiv - Microbiology 2021Quote: ... and eluted in 150 µL wash buffer with 500 ng/µL FLAG peptide (Merck) by rotation with a rotator for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were eluted with 20 μl of 1 mg/ml 3xFLAG peptide (Merck, F4799) for 15 min at room temperature with vigorous shaking ...
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bioRxiv - Immunology 2019Quote: ... HLA molecules and peptides were eluted with 1% trifluoroacetic acid (TFA, Merck, Darmstadt, Switzerland) directly into Sep-Pak tC18 100 mg Sorbent 96-well plates (Waters ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were extracted in 70% ethanol plus 5% formic acid (Merck-Millipore) twice for 20 min with permanent shaking ...
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bioRxiv - Immunology 2022Quote: ... HLA-peptide complexes were eluted by repeated addition of 0.2% TFA (trifluoroacetic acid, Merck). Eluted HLA ligands were purified by ultrafiltration using centrifugal filter units (Millipore) ...
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bioRxiv - Immunology 2020Quote: ... HLA-peptide eluates were loaded onto C18 RP-HPLC column (Chromolith Speed Rod; Merck). The bound peptides were separated using increasing concentration of 80% acetonitrile and 0.1% trifluoroacetic acid (80% ACN / 0.1% FA) ...
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bioRxiv - Microbiology 2022Quote: AB19026 ADAM10 rabbit polyclonal antibody (Immunogen: hADAM10 peptide 732-748) was purchased from Merck; anti-ADAM10 IC1427F was from R&D ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Tryptic peptides were acidified to a final concentration of 1% formic acid (FA) (Merck), cleaned up using SepPak cartridges (Waters) ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated MHC class II-peptide complexes were purified on a HisTrap HP column (Merck) and kept at -80°C until multimerization with streptavidin conjugates.
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bioRxiv - Developmental Biology 2023Quote: ... 10 µg of peptides were further desalted with C18 ZipTips (Merck Millipore, Darmstadt, Germany) to ensure complete removal of beads.
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bioRxiv - Cell Biology 2023Quote: ... The concentration of peptides was measured by a Direct Detect Spectrophotometer (Merck, Darmstadt, Germany), and solutions were made up to 100 μg in 100 μl of injection solution prior to desalting.
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bioRxiv - Cell Biology 2023Quote: ... peptide concentration was estimated via Direct Detect® Infrared Spectrometer (Merck, Bayswater, VIC, Australia), and samples were transferred to HPLC vials for proteomics analysis.
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bioRxiv - Immunology 2020Quote: ... After washing and incubation with unbuffered DMEM containing 25 mM D-Glucose (Carl Roth, Karlsruhe, Germany) and 1 mM Pyruvate (Merck) for 1h at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were then vacuum concentrated (Labconco Centrivap) and peptides purified by C18 ZipTips® (Merck) prior to analysis by LC-MS/MS or LC-SWATH-MS ...
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bioRxiv - Systems Biology 2022Quote: ... The procedure was performed by manufacturer’s instructions including peptide purification with C18 ZipTips® (Merck). The eluted peptides were snap frozen and lyophilized before reconstitution in 0.1% formic acid for HPLC-MS/MS.
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bioRxiv - Cell Biology 2023Quote: ... The peptides were desalted and fractionated using 200 µl Pierce C18 column tips (Merck Millipore).
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were then desalted using styrene divinylbenzene-reverse phase sulfonate Stage-tips (Merck, Darmstadt, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% (v/v) fetal bovine serum, 1 mM sodium pyruvate (gibco, 11360039) and 1% antibiotic/antimycotic solution (Merck, A5955). Medium was refreshed every 3 days during cultivation and every 24h in an experimental setting ...
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bioRxiv - Immunology 2024Quote: ... U-937 cells were maintained in RPMI-1640 medium supplemented with 4.5 g/L glucose, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1.0 mM sodium pyruvate (Sigma-Aldrich; Merck KGaA), 10% fetal bovine serum (Hyclone ...
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bioRxiv - Neuroscience 2021Quote: ... The Aβ stock solution was prepared by solubilizing the Aβ25-35 peptide (Sigma-Aldrich; Merck KGaA) in sterile deionized water to a concentration of 1 mM and then incubated at 37°C for 3 days to allow self-aggregation before treatment ...
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bioRxiv - Neuroscience 2020Quote: ... The Aβ stock solution was prepared by solubilizing the Aβ25-35 peptide (Sigma-Aldrich; Merck KGaA) in sterile deionized water to a concentration of 1 mM and then incubated at 37°C for 3 days to allow self-aggregation before treatment ...
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bioRxiv - Biochemistry 2020Quote: ... Flag-tagged proteins were eluted by incubation with 150 μg/mL Flag tag peptide (Merck; F3290) with 0.1% digitonin in solubilisation buffer for 30 min at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... The digested peptides were further filtered with Amicon ultra centrifugal filters (10kD MWKO, Merck Millipore, MA), dried under vacuum extensively to remove residual primary amine from reagents ...