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Citations for Merck :
101 - 150 of 1096 citations for Herpes Simplex Virus 2 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... protein lysates were prepared using 1X RIPA buffer containing protease (#539131, Merck Millipore) and phosphatase (#524629 ...
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bioRxiv - Immunology 2023Quote: ... Colony-forming units (CFU) were enumerated in tumour lysates on LB agar (Merck) using standard microbiological techniques.
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bioRxiv - Microbiology 2023Quote: ... The remaining 90% of lysate was incubated with anti-Flag antibody (Merck, F1804) coupled to Protein G Dynabeads (ThermoFisher ...
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bioRxiv - Systems Biology 2023Quote: Caspase-9 activity was measured in liver lysate according to manufacturer’s protocol (Merck Caspase-9 Colorimetric Activity Assay Kit APT139 ...
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bioRxiv - Microbiology 2023Quote: ... The remaining 90% of lysate underwent immunoprecipitation with anti-Flag antibody (Merck, F1804) coupled to Protein G Dynabeads (ThermoFisher ...
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bioRxiv - Neuroscience 2021Quote: ... Microtubule-Associated Protein 2 (MAP-2) (Merck Millipore Burlington ...
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bioRxiv - Cell Biology 2023Quote: ... 2% glucose + 2% D/L-lactate (Merck), or 2% D/L-lactate alone [31].
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bioRxiv - Neuroscience 2021Quote: ... coverslips containing fixed neurons were permeabilized and non-specific antigens blocked by incubating in D-PBS with 0.2% Triton-X (Merck, cat. X100-500ML) and 10% donkey serum (Merck ...
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bioRxiv - Microbiology 2022Quote: ... was applied onto the dried treatment (10mL of virus was concentrated using centrifugation through Amicon® Ultra-15 Centrifugal Filter Units (Merck). After 5 minutes at room temperature 150μl of DMEM (high glucose ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Microbiology 2020Quote: ... Mueller Hinton broth 2 (MHC-2, Merck, USA) for bacitracin susceptibility tests ...
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bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck) to stop the fixation reaction.
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bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck) and subsequently allowed to adhere on ICAM-1 coated glass slides before TOCCSL imaging ...
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bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck)) ...
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bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck). Cells were kept on ice or seeded onto ICAM-1-coated glass slides for imaging at 37°C and in TIRF mode ...
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bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck) and afterwards incubated with excess amounts of mSav-cc-PS-CFP2 for 30 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck) and subsequently allowed to adhere on ICAM-1 coated glass slides prior to imaging in TIRF mode at 22.5°C ...
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bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck)) ...
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bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck)) and kept on ice until imaging proceeded ...
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bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck)) prior to imaging.
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bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck). Cells were allowed to adhere on ICAM-1 coated glass slides for 10 minutes at 25°C before fixation ...
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bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck) prior to seeding the cells onto ICAM-1-coated glass-slides ...
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bioRxiv - Immunology 2019Quote: ... sections from the initial portions of sockets were incubated with primary antibody for Proliferating Cell Nuclear Antigen (PCNA) (#MAB424, Merck Millipore, Darmstadt, Germany) diluted to 1:150 in blocking solution and incubated at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... 500 μl of the phage lysate was treated with DNAse-I (Merck cat #11284932001) added to a final concentration of 20 μg/ml and incubated at 37°C for 1 hour to remove bacterial DNA ...
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bioRxiv - Cancer Biology 2020Quote: ... The lysates were separated through SDS-PAGE and transferred to nitrocellulose membranes (Merck Millipore), blocked in 5% milk in TBS with 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2022Quote: ... 500 µl of the phage lysate was treated with DNase-I (Merck cat #11284932001) added to a final concentration of 20 µg/ml and incubated at 37C for 1 hour to remove bacterial DNA ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mg chromatin lysates were incubated with 50 µl FLAG M2 beads (Merck A2220) for 2 h at 4°C with rotation ...
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bioRxiv - Developmental Biology 2022Quote: ... the lysate was concentrated on an Amicon Ultra Centrifugal Filter (Merck Millipore; Billerica, MA), 10μl of the concentrated lysate was resolved on a 4-12% NuPAGE Bis-Tris gel and transferred onto a PVDF membrane using the iBlot system (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... The total lysate was exchanged into PBS buffer using centrifugal filter devices (Merck, UFC201024) according to manufacturer protocols.
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bioRxiv - Microbiology 2022Quote: ... 500 µL of the phage lysate was treated with DNase-I (Merck cat #11284932001) added to a final concentration of 20 mg mL−1 and incubated at 37°C for 1 h to remove bacterial DNA ...
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bioRxiv - Biochemistry 2023Quote: ... Resulting crude lysates were then sonicated for 5 min in an ultrasonic bath (Merck) for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 ml of phage lysate was treated with DNase-I (Merck catalogue no. 11284932001) added to a final concentration of 20 µg/ml and incubated at 37 °C for 1 h to remove bacterial DNA ...
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bioRxiv - Genetics 2023Quote: ... 500 µl of the phage lysate was treated with DNase-I (Merck cat #11284932001) added to a final concentration of 20 mg ml−1and incubated at 37°C for 1 hour to remove bacterial DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Cell Biology 2023Quote: ... the reducing agent 2 µl 2-picoline borane complex (PB, 2 M in DMSO, Merck) and 2 µl water ...
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bioRxiv - Neuroscience 2020Quote: ... individual COs were transferred to an Eppendorf tube containing CMM medium with 1:100 of virus containing medium and 5µg/ml Polybrene (Merck; TR-1003-6) and incubated over-night ...
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bioRxiv - Molecular Biology 2021Quote: hEnSC were seeded at a confluency of 105 cells/well in 6 well plates and 1.5 ml of concentrated virus added to each well in the presence of 8 μg/ml polybrene (Merck, TR-1003-G). Cells were then spinoculated at 500 x g for 1 hr at RT and incubated for 12 hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... hTERT cells were transduced with 1.5 ml of concentrated pMY-SUZ12-FLAG-IRES-bls virus in the presence of 8 μg/ml polybrene (Merck, TR-1003-G). Cells were then spinoculated at 500 x g for 1 hr at RT and incubated for 12 hrs ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium containing the virus was collected 48 hours post-transfection and filtered through a 0.45 µm polyethersulfone (PES) filter (Merck Millipore, Billerica, MA).
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bioRxiv - Neuroscience 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (all Merck). The pH was adjusted to 7.3-7.4 (S20 ...
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bioRxiv - Biophysics 2023Quote: ... 2-aminoethoxydiphenyl borate (2-APB) (Sigma-Aldrich/Merck, Germany), BL-1249 ...
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The lysate was then mixed with 1 volume of phenol-chloroform-isoamylalcohol (Merck cat #P2069), and centrifuged for at maximal speed in a table top centrifuge at 4°C for 15 min ...
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bioRxiv - Immunology 2019Quote: Protein lysates from cells were obtained by lysis in radioimmunoprecipitation assay buffer (Merck Millipore, #R0278) with protease and phosphatase inhibitors (Roche ...
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bioRxiv - Immunology 2019Quote: ... Cell lysates were separated by SDS-gel electrophoresis and transferred to PVDF membranes (Merck Millipore). Immunoblotting was performed using the following antibodies from Cell signaling ...
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bioRxiv - Cell Biology 2019Quote: ... protein lysates were cleared by centrifugation and protein concentration was measured using Coomassie reagent (Merck). Denatured ...
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bioRxiv - Pathology 2023Quote: ... 48h after transfection rAAV8 vectors were harvested from cell lysate and treated with Benzonase (Merck) at 100U/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... the lysate was incubated in the presence of BG4 (Merck, Milan, Italy, MABE817, Lot: 3534080). Anti-FLAG antibody (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentivirus of pooled sgRNA phosphatase library titer was estimated by mixing the cells with the serially diluted virus and measuring GFP% on day three post-infection using a Guava® easyCyte™ Flow Cytometer (Merck Millipore). The cells were then infected with the volume of virus estimated to result in a 30–40% infection rate to increase the probability of a single sgRNA introduction event per cell ...
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bioRxiv - Cancer Biology 2022Quote: ... Target cells (MDA, A549 and HCT) were then infected with the produced virus using 2μg/ml polybrene (Merck, Darmstadt, Germany, #TR-1003-G). Stably transduced cells were either selected by flow cytometry using BD FACSAria III cell sorter or 1μg/ml puromycin (InvivoGen ...