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Citations for Merck :
1 - 50 of 1096 citations for Herpes Simplex Virus 2 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... SV40 T Antigen (Ab-2) (Merck Millipore; DP02; 5 μg/ml). Secondary antibodies (all from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and NG2 (neuron-glial antigen 2, AB5320, Merck Millipore, Burlington, MA, USA, 1:400) were used.
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bioRxiv - Molecular Biology 2024Quote: ... Cumulus cells were removed by briefly culturing the zygotes in potassium simplex optimization medium (KSOM) (catalog no. MR-101-D, Merck) supplemented with 0.3 µg.µL-1 hyaluronidase (catalog no ...
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bioRxiv - Developmental Biology 2022Quote: ... Antigen retrieval in citrate buffer (Merck) was followed by permeabilization with Triton X-100 (Merck ...
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bioRxiv - Microbiology 2022Quote: ... the Milliplex SARS-CoV-2 Antigen Panel 1 IgG was used according to the manufacturer’s instructions (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2019Quote: ... mouse anti-human nuclear antigen (1:100; Merck), Alexa Fluor 647-conjugated rat anti-CD45 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... recombinant hepatitis B surface antigen (HBsAg) manufactured by Merck & Co. ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was then incubated with 2 mL pre-washed HIS-Select Cobalt Affinity Gel (Merck, UK) with rotation for an hour ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-flavivirus group antigen (1:800, MAB10216, Merck Millipore) and goat anti-Iba1 (1:300 ...
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bioRxiv - Neuroscience 2024Quote: ... Total 2 mg lysate (1 ml) was incubated with 10 µl of anti-FLAG M2 Magnetic Beads (Merck), which was pre-washed twice with IP buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... the virus-containing supernatant (8ml) was filtered (Steriflip; Merck) before being concentrated 20x (14000 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... was performed on 4 and 2.5 mg protein lysates for timsTOF optimization and dual-probe analyses respectively in 2 M urea (Merck) in 1× 50 mM HEPES (pH 7.5) ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated with 2.5 µg/mL soluble HER2 antigen (Merck) and then with 1:250 APC-labeled anti-human HER2 antibody (Biolegend) ...
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bioRxiv - Bioengineering 2022Quote: ... Antigen retrieval was carried out with pronase (3.5 U/ml; Merck) at 37 °C for 25 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... lysates (including phosphatase inhibitors (Merck) in the case of the quantitative γH2A-assay ...
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bioRxiv - Synthetic Biology 2021Quote: ... HER2 binding was confirmed with 2.5 µg/mL soluble HER2 antigen (Merck) and subsequent 1:200 APC-labeled anti-HER2 antibody (Biolegend ...
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bioRxiv - Microbiology 2020Quote: ... a commercial mouse anti-flavivirus group antigen monoclonal antibody (MAB10216; Merck Millipore) diluted 1:1,000 in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2021Quote: ... The virus was concentrated in a 100KDa Amicon centrifugation unit (Merck) at 5000g for 15 mins to achieve a 10-fold concentration ...
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bioRxiv - Neuroscience 2021Quote: ... This concentrated virus solution was filtered (0.22 μm Millex, Merck Millipore) and stored at 4 °C until further use ...
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bioRxiv - Evolutionary Biology 2023Quote: ... virus samples were mixed with SYBR Green I stain (Merck, Germany) and Tris-EDTA buffer in a PCR plate ...
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bioRxiv - Neuroscience 2024Quote: ... Surface antigens were labeled with primary antibody (rabbit anti-GFP, 1:1000, Merck) for 15 min on ice ...
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bioRxiv - Microbiology 2021Quote: IFN-α and IFN-β or IL-6 and TNF-α were measured in supernatants of AMs or lung lysates using IFN alpha/IFN beta 2-Plex Mouse ProcartaPlex™ immunoassay (ebiosciences) or Milliplex MAP Mouse™ assay (Merck), respectively ...
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bioRxiv - Microbiology 2020Quote: ... virus-containing supernatants were filtered through a 0.45 μm membrane (Merck Millipore). The amount of virus in the supernatants was quantified by RT assay ...
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bioRxiv - Cell Biology 2022Quote: ... Virus amplification was performed in Sf9 TriEX cells (Novagen / Merck #71023-3) grown in TriEx media (Novagen / Merck #71022) ...
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bioRxiv - Genetics 2022Quote: ... Virus was concentrated using Amcion Ultra-15 30k centrifugal filter (Merck#C7715) and centrifugation for 30 min at 3,900rpm.
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bioRxiv - Genomics 2024Quote: ... virus-containing supernatant was filtered through a 0.45 μm filter (Merck Millipore), aliquoted ...
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bioRxiv - Microbiology 2022Quote: ... A PgVV-only lysate was obtained by 0.2 µm filtering of a mixed lysate (Millex, SLGV033RS, Merck Millipore), TFF concentration (Repligen N06-E100-05-N ...
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bioRxiv - Biochemistry 2021Quote: ... incubated with ReBlot Plus strong antigen retrieval agent (Merck, Watford, UK, cat. no. 2504) for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... protein lysates were reduced with dithiothreitol (Merck) and cysteine residues were alkylated with iodoacetamide (Merck) ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated using Amicon Ultra-4 centrifugal filter units (Merck-Millipore) and the solution was filtered with a 0.2 µm Acrodisc syringe filter (Sigma-Aldrich).
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bioRxiv - Physiology 2021Quote: ... The virus was further purified using Sepharose G100 SF resin (Merck, Darmstadt, Germany) in Econopac colums (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... Virus was concentrated in PBS using Amicon Ultra-15 Centrifugal Filter Units (Merck) and stored at 4°C.31,77,78 Viral titre was quantified by inverted terminal repeat probe quantitative polymerase chain reaction (PCR) ...
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bioRxiv - Biochemistry 2023Quote: ... Virus- containing supernatant P1 was sterile-filtered using a 0.22 μm filter (Merck). To amplify the virus 1.5 mL P1 was added to 250 mL of Sf21 shaking cell culture at a density of 0.4*106 cells/mL ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μl AAV virus was mixed with 1 μl 20% mannitol (MERCK K93152782 111). The virus and mannitol mixture were injected into a pulled-glass pipette (Warner Instruments ...
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bioRxiv - Biophysics 2023Quote: ... concentrated virus particles were freshly thawed and diluted in phosphate-buffered saline (PBS, Merck, 806552 ...
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bioRxiv - Cell Biology 2024Quote: ... of cell lysates by incubating lysates with 0.5-2.5 µg of antibodies and 20 µl of protein G–Sepharose (Merck, GE17-0618-01) at 4°C for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... Antigen retrieval was carried out with 0.2 U/mL Chondroitinase ABC (C2905, Sigma, Merck, Darmstadt, Germany) at 37 °C for two hours for DCN ...
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bioRxiv - Microbiology 2020Quote: ... The released antigen was then concentrated with the Amicon 3 kDa filter (Merck KGaA, Darmstadt, Germany) before being analysed by SDS gel electrophoresis ...
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bioRxiv - Genomics 2020Quote: ... An amount of 35 or 40 μg whole cell lysates or tissue lysates were resolved by 10% SDS-PAGE and transferred onto PVDF membranes (Merck Millipore, Germany). After incubation in 5% non-fat milk for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Virus-infected culture was mixed with an equal volume of 0.4% Gelzan™ CM (Merck) and spread onto pre-warmed 0.7% Gelzan™ CM/SCV plates ...
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bioRxiv - Microbiology 2020Quote: ... virus-containing medium from producing cells was treated with 15 U/ml DNase I (Merck) and 10 mM MgCl2 for 5 h at 37°C prior to ultracentrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was added to RPE1-hTERT cells together with 8µg/ml polybrene (Merck Life science). Cells were selected using 5 µg/ml puromycin (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing cell supernatants were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore) and exchanged into TexMACS media (Miltenyi Biotec).
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bioRxiv - Microbiology 2023Quote: ... virus stocks were concentrated on a 100 KDa cellulose membrane (Amicon Ultra-15, Merck Millipore) and rinsed three times successively with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... virus at the MOI of 50 was applied with 8 μg/ml Polybrene (Merck Millipore). Virus-containing medium was replaced with medium containing puromycin (3.5 μg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclear lysates were treated with Benzonase (Merck-Millipore, 70664). Nuclear lysates were pre-cleared on 25 μL Dynabeads M-280 Sheep anti-mouse or antirabbit IgG (Thermo ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysates were prepared using RIPA buffer (Merck/Millipore). SDS-PAGE and western blotting were performed using standard protocols ...
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bioRxiv - Biochemistry 2020Quote: ... MEFs were immortalized with SV40 large T-antigen by transfection using GeneJuice transfection reagent (Merck Millipore 70967).
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bioRxiv - Neuroscience 2020Quote: ... Sections were deparaffinized and submitted to antigen retrieval by incubating the slides in sodium citrate (Merck KGaA) 10mM pH 6.0 for 1 h in a 95°C water bath ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The virus supernatants were directly overlaid on H1299 cells in the presence of polybrene (Merck, Germany) at a final concentration of 4 μg/ml ...