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Citations for Merck :
151 - 200 of 2878 citations for Dengue Virus serotype 1 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: Proteins in cell lysates were separated in a 10% SDS-PAGE and transferred to Immobilon NC (Millipore Merck, Darmstadt, Germany) membranes ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was filtrated using Millex® HV membrane filter units with a pore size of 0.45 µm (Merck Millipore). Fy-4 was subsequently purified combining Ni-NTA affinity chromatography (HisTrap FF column ...
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bioRxiv - Cell Biology 2022Quote: Proteins (either from whole cell lysates or insoluble fraction) were resolved by SDS-PAGE and transferred to PVDF membrane (Merck) by western blotting ...
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bioRxiv - Cell Biology 2023Quote: ... Ninety μl of cell lysates were mixed with 10 μl of lysis buffer containing 0.1 μl benzonase (Merck Millipore, 70664) and further incubated on ice for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitation of cell lysates overexpressing FLAG-tagged or GST-tagged proteins were performed using anti-FLAG-M2 affinity beads (Merck) or glutathione-Sepharose beads (Cytiva).
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bioRxiv - Microbiology 2024Quote: ... Proteins in the lysate were then fractionated by SDS-PAGE and transferred to a PVDF membrane (Merck Millipore, Cat#IPVH00010). After blocking with 1% nonfat milk overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 500µg of cell lysates were incubated with pre-washed EZview Red ANTI-HA or FlagM2 affinity Gel Beads (E6779 and F2426, Merck) at 4 °C under overnight rotation ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were treated with 100 μl of 10 mg/ml Heparin and 10 μl of Benzonase (25 U/μl, Merck Millipore) for 15 minutes at room temperature before removing cell debris by centrifugation at 31,000 g for 20 minutes and ultracentrifugation at 208,000 g for 1.5 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell lysates were clarified by centrifugation and a minimum of 200μg of lysate was incubated with pre-washed EZview™ Red ANTI-FLAG® M2 Affinity Gel (F2426, Merck) at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: Antibody-bead mixture was washed twice in iCLIP lysis buffer to remove unbound antibody and RNase-treated cell lysates were added alongside cOmplete EDTA-free Protease Inhibitor Cocktail (Merck, #11836170001) and incubated for 2 hours at 4°C on a rotating wheel ...
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bioRxiv - Microbiology 2022Quote: ... Proteins in cell lysates were separated by 10% SDS-PAGE and transferred to Immobilon NC membranes (Millipore Merck KGaA, Darmstadt, Germany). The membranes were blocked by incubation with 5% nonfat dry milk in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Whole-cell lysate was loaded and separated on a 7.5% polyacrylamide gel and transferred onto a PVDF membrane (Merck Millipore, IPVH07850). Membranes were blocked with PVDF Blocking Reagent for Can Get Signal (Toyobo ...
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bioRxiv - Microbiology 2023Quote: ... Clear lysates were subsequently filtered through 10 kDa followed by 3 kDa Amicon Ultra-0.5 centrifugal filters (Merck KGaA, Darmstadt, Germany) to generate sub-cellular fractions with molecular size of larger than 10 kDa ...
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bioRxiv - Microbiology 2022Quote: ... Three microliters of cell suspension (whole cell) or cell lysate were spotted on an Immobilon-PSQ transfer membrane (Merck Millipore Ltd.) and air dried ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from cell lysates were separated via SDS-PAGE and transferred to Immobilon-P PVDF membranes (IPVH00010, Merck & Co., Inc., USA). These membranes were subsequently probed with the indicated primary antibodies and incubated with appropriate goat anti-rabbit IgG or goat anti-mouse IgG (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysate was prepared in 1X CHAPS Lysis Buffer and the assay was performed using TRAPeze™ RT Telomerase Detection Kit (Merck) per manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... The cell lysate was then separated by electrophoresis in a 10% polyacrylamide gel and transferred to a PVDF membrane (Merck Millipore). The membrane was blocked with 5% milk in PBS-T ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was diluted in 10 ml of 1X PBS buffer and filtered using an Amicon 50 kDa filter column (Merck, Darmstadt, Germany) to concentrate the extract to a final volume of 500 μL.
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Microbiology 2021Quote: IFN-α and IFN-β or IL-6 and TNF-α were measured in supernatants of AMs or lung lysates using IFN alpha/IFN beta 2-Plex Mouse ProcartaPlex™ immunoassay (ebiosciences) or Milliplex MAP Mouse™ assay (Merck), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the cell lysates was measured using the BCA assay and insulin levels were quantified by radioimmunoassay (rat insulin RIA kit, Merck, Darmstadt, Germany).
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bioRxiv - Immunology 2022Quote: ... A 15 ul of lysate was resolved on 12% SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore, Burlington, MA). The membrane was blocked in 5% powder milk overnight in 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysate was clarified by high-speed centrifugation and then purified on 500 μL of Glutathione Sepharose 4B resin (Merck, GE-17-0756-01). Beads were pre-equilibrated in Lysis Buffer and supernatant was incubated with resin for 2h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... and proteins in the lysate were separated in a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). The membrane was incubated with 5 % skim milk (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amount of lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride membrane (Merck Millipore, Burlington, MA, USA) in 25 mmol/L Tris ...
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bioRxiv - Microbiology 2023Quote: Proteins in the lysate were separated on a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). The membrane was incubated with 5 % skim milk (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... α: β: γ: δ = 1:1:1:1 was obtained from Merck (Darmstadt, Germany).
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bioRxiv - Immunology 2021Quote: ... 1 μM Src inhibitor-1 (Merck), 2.5 μM Src inhibitor PP1 (Cayman Chemical ...
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bioRxiv - Microbiology 2023Quote: ... 1 g.L-1 L-cysteine (Merck) and 1 g.L-1 NaHCO3 (Carl Roth GmbH ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 µM Ferrostatin-1 (Merck, #SML0583), 10 µM oleic acid (Merck ...
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bioRxiv - Cell Biology 2020Quote: ... anti-HES-1 (1:500, 1% casein, #AB5702, Millipore, Merck, Darmstadt, Germany), anti-LRP6 (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% glutamine and 1% penicillin/streptomycin (Merck). Fascin KD cells were selected and maintained using puromycin ...
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bioRxiv - Neuroscience 2020Quote: ... GluA1 (1:250-1:500; Merck Millipore) and RTP801 (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... 1-methyl-D-tryptophan (1-DMT; 1-LMT enantiomer, both Merck Life Sciences) for exploration of a possible tryptophan mechanism of immunosuppression ...
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bioRxiv - Bioengineering 2019Quote: ... 1×bugbuster and 1% lysonase (Merck, Hertfordshire, UK) in 50 mM Tris-HCl ...
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CAP1 and cofilin1 cooperate in neuronal actin dynamics, growth cone function and neuron connectivitybioRxiv - Neuroscience 2020Quote: ... mouse anti tau-1 (1:200, Merck Millipore), mouse anti-c-Myc (1:200 ...
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bioRxiv - Molecular Biology 2020Quote: ... RAD51 (Merck, Ab-1, PC130, dilution 1:1000), γH2AX (Millipore ...
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bioRxiv - Developmental Biology 2021Quote: ... 1X complete protease inhibitor (Roche)] including 1% SDS and 1 U/µL benzonase (Merck). After 10 minutes of incubation at RT ...
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bioRxiv - Cell Biology 2021Quote: ... GLUT-1 (rabbit polyclonal, Merck Millipore, #400060, 1:100), Ki-67 (rabbit monoclonal ...
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bioRxiv - Cell Biology 2022Quote: ... human mitochondria (clone 113-1, Merck Millipore, 1:100), and BrdU (clone BU1/75 ICR1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Puromycin (cat# MABE343, Merck Millipore; 1:2,000-1:10,000), anti-Ubiquitinylated proteins,clone FK2 (cat# 04-263 ...
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bioRxiv - Biophysics 2022Quote: ... and 1 mg ml-1 lysozyme (Merck/Sigma–Aldrich). After 1 h incubation while stirring ...
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bioRxiv - Developmental Biology 2022Quote: ... 1/10 volume of 1% IGEPAL CA630 (Sigma, Merck) was added to the samples and incubated for additional 3 minutes at room temperature ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... mouse anti-Centrin (1:500-1:1000, Merck Millipore), chicken anti-GFP (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... anti-α-tubulin (Merck, 1:1000, TAT-1 00020911), anti-GFP (ChromoTek ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mM AEBSF) and 1 protease inhibitor tablet (Merck). Lysis was done at 4 °C with the addition of 1 mg/ml lysozyme and further incubation at 4 °C for 30 minutes ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies (FUS 1:100 Santa Cruz Biotech sc-47711; ISL1 1:500 Abcam ab109517; OLIG2 1:100 R&D Systems AF2418; NFM 1:1000 Merck MAB1621) were added in 1% BSA and incubated at 4degC overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Tubulin beta (1:8,000; clone KMX-1 from Merck Millipore), dMi-2 (1:8,000 ...
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bioRxiv - Neuroscience 2019Quote: ... anti-vGlut (Merck Millipore, cat MAB5502, 1:200; 1:1,000); anti-GluR2 (Merck Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... and anti-TUJ-1 antibodies (1:200; Merck-Millipore, MAB380) for 48 h ...