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Citations for Merck :
51 - 100 of 2878 citations for Dengue Virus serotype 1 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Protein concentrations of lysates were determined using BCA assay (Merck). Proteins in culture supernatants were precipitated using methanol/chloroform ...
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bioRxiv - Cancer Biology 2019Quote: ... The virus was harvested 48 hours post transfection and filtered through a 0.45 μm PES filter (Merck-Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... Respiratory syncytial virus RSV-G was immunoblotted with commercially available goat polyclonal anti-RSV serum (#AB1128, Merck). Coxsackievirus VP1 was immunoblotted with commercially available mouse monoclonal anti-VP1 (3A8 ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... virus was also concentrated using Amicon Ultra-15 Centrifugal Filter Units with 100 kDa cutoff (Merck Millipore). Mice were injected s.c ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 mL of virus supernatant is concentrated using Amicon Ultra-4 30K centrifugal filters (Merck, cat# UFC803024). For transduction of suspension cells ...
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bioRxiv - Neuroscience 2019Quote: ... the cell lysates were treated with benzonase nuclease (Merck, Darmstadt, Germany) at 37°C for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... the cell lysates were treated with benzonase nuclease (Merck, Darmstadt, Germany) at 37°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were supplemented with 500 U Recombinant DNAse (Merck 4716728001) and 100 mM PMSF ...
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bioRxiv - Neuroscience 2021Quote: ... The cell lysate was treated with Benzonase (75 U/μl; Merck) for 1h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The phage lysate was treated with DNase-I (Merck cat #11284932001) added to a final concentration of 20 μg/ml and incubated at 37°C for 1 hour to remove bacterial DNA ...
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bioRxiv - Cell Biology 2023Quote: ... cell lysates were precipitated on magnetic carboxylate modified beads (Merck, GE45152105050250) in the presence of 77% (volv/vol ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was filtrated in Centricon™ 10 kDa (Merck Millipore). The flow-through was loaded into SPE-C18 (MacroSpin Columns ...
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bioRxiv - Developmental Biology 2023Quote: ... the lysate was digested with proteinase K (Merck, Kenilworth, NJ, USA) at 55°C for 2 h ...
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bioRxiv - Biophysics 2024Quote: ... The phages were then purified with two different protocols: Protocol 1: Phage lysates were filtered through 0.22 µm polysulfone membrane filters (Merck Millipore, Billerica, MA, USA) and were used for subsequent purification and concentration by cross-flow filtration using Sartorius Hollow fiber cartridges ...
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bioRxiv - Microbiology 2020Quote: ... and replaced with an appropriate amount of virus diluted in 0.5 mL of Dulbecco’s modified Eagle medium (Merck) with 1% FCS ...
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bioRxiv - Microbiology 2020Quote: ... virus inoculum was removed from each well and cells were overlaid with 300 μL of 0.6% carboxymethylcellulose (Merck) diluted in DMEM supplemented with 2% FBSi ...
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bioRxiv - Neuroscience 2020Quote: ... the cell lysate was treated with 250 U/ml benzonase nuclease (Merck) and 1 mM MgCl2 for 10−15 min at 37 °C and centrifuged at 4000 rpm for 20 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Protein lysates were concentrated using Microcon-10kDa Centrifugal Filter Units (Merck Millipore), according to manufacturer’s instructions and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... Total protein was isolated from whole cell lysates using BugBuster® (Merck). Total protein concentrations were quantified by Pierce BCA quantification kit (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was loaded onto a 5 mL HisTrap FF column (Merck), washed with buffer A (50 mM Tris-Cl pH 8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... the cell lysate was treated with 250 U/ml benzonase nuclease (Merck) at 37 °C for 10 − 15 min with adding 1 mM MgCl2 and then centrifuged at 4°C at 1753×g for 20 min ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... the cell lysate was treated with 250 U/ml benzonase nuclease (Merck) at 37 °C for 10–15 min with adding 1 mM MgCl2 and then centrifuged at 4 °C at 1753×g for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... the chromatin lysate was directly wicked onto Amersham Protran nitrocellulose membrane (Merck) by pipetting small volumes above the membrane ...
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bioRxiv - Cell Biology 2023Quote: ... lysates with equal protein concentration were incubated with Rabbit IgG (Merck, control) and 50 μg of Stwl antibody overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The lysate was loaded onto a Fractogel DEAE anion exchange column (Merck). PTE elutes in the flow-through ...
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bioRxiv - Genetics 2023Quote: ... Protein lysate was prepared in Radio-immunoprecipitation assay (RIPA) buffer (Merck Millipore) with a protease inhibitor cocktail (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... 293T or 293GP virus producing cells were transfected using the GeneJuice reagent according to the manufacturer’s recommendations (Merck Millipore). Two days after transfection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... One milliliter of the virus supernatants was directly overlaid on the cells in the presence of polybrene (Merck, Germany) at a final concentration of 4 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... 96-well plates with confluent cell layers were fixed and virus was inactivated by immersion in 100% methanol (MERCK) for 20 min ...
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bioRxiv - Genomics 2023Quote: ... The transduction was performed by adding the virus particle directly onto the cells supplemented with 6μg/mL polybrene (Merck). The cells were refreshed 24h after transduction and the antibiotics (puromycin/blasticidin ...
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bioRxiv - Microbiology 2021Quote: ... the pUL21 gene was amplified from virus stock by PCR with the oligonucleotide primers 5′-ATGGAGCTTAGCTACGCCAC-3′ and 5′-TTTATTGGGGTCTTTTACACAGACTGTC-3′ using KOD polymerase (Merck) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... For detection of particle incorporation virus supernatant was further concentrated by centrifugation at 4°C for 2h at 21000xg on 5% Optiprep (Merck), the supernatant was removed and pellet was resuspended and subjected to Western Blot analysis ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration of each lysate was determined using a BCA assay (Merck, 71285).
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bioRxiv - Microbiology 2021Quote: ... 0.5 ml of phage lysate was treated with DNase-I (Merck cat #11284932001) added to a final concentration of 20 ug/ml and incubated at 37°C for 1 hour to remove bacterial DNA ...
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bioRxiv - Microbiology 2021Quote: ... 500 μl of the phage lysate was treated with DNaseI (Merck cat #11284932001) added to a final concentration of 20 μg/ml and incubated at 37°C for 1 hour to remove bacterial DNA ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was loaded overnight onto a 5mL HisTrap HP column (Cytiva, Merck) equilibrated in 20 mM Hepes pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Cleared cell lysate was filter with 0.45 µm sterile filter (Millipore Merck, Schwalbach) and loaded onto a 5 ml HiTrap Chelating HP column (GE Healthcare ...
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bioRxiv - Synthetic Biology 2022Quote: The cell lysate was loaded on 10 mL D-mannose-agarose resin (Merck) pre-equilibrated with Buffer A ...
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bioRxiv - Synthetic Biology 2022Quote: ... the cell lysate was loaded on 10 mL D-mannose-agarose resin (Merck) pre-equilibrated with Buffer A ...
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bioRxiv - Cancer Biology 2023Quote: ... protein lysates were prepared using 1X RIPA buffer containing protease (#539131, Merck Millipore) and phosphatase (#524629 ...
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bioRxiv - Immunology 2023Quote: ... Colony-forming units (CFU) were enumerated in tumour lysates on LB agar (Merck) using standard microbiological techniques.
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bioRxiv - Microbiology 2023Quote: ... The remaining 90% of lysate was incubated with anti-Flag antibody (Merck, F1804) coupled to Protein G Dynabeads (ThermoFisher ...
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bioRxiv - Systems Biology 2023Quote: Caspase-9 activity was measured in liver lysate according to manufacturer’s protocol (Merck Caspase-9 Colorimetric Activity Assay Kit APT139 ...
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bioRxiv - Microbiology 2023Quote: ... The remaining 90% of lysate underwent immunoprecipitation with anti-Flag antibody (Merck, F1804) coupled to Protein G Dynabeads (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... was applied onto the dried treatment (10mL of virus was concentrated using centrifugation through Amicon® Ultra-15 Centrifugal Filter Units (Merck). After 5 minutes at room temperature 150μl of DMEM (high glucose ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Microbiology 2020Quote: ... 500 μl of the phage lysate was treated with DNAse-I (Merck cat #11284932001) added to a final concentration of 20 μg/ml and incubated at 37°C for 1 hour to remove bacterial DNA ...
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bioRxiv - Cancer Biology 2020Quote: ... The lysates were separated through SDS-PAGE and transferred to nitrocellulose membranes (Merck Millipore), blocked in 5% milk in TBS with 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2022Quote: ... 500 µl of the phage lysate was treated with DNase-I (Merck cat #11284932001) added to a final concentration of 20 µg/ml and incubated at 37C for 1 hour to remove bacterial DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... the lysate was concentrated on an Amicon Ultra Centrifugal Filter (Merck Millipore; Billerica, MA), 10μl of the concentrated lysate was resolved on a 4-12% NuPAGE Bis-Tris gel and transferred onto a PVDF membrane using the iBlot system (Invitrogen ...