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Citations for Merck :
601 - 650 of 2598 citations for Centrosomal Protein 63 kDa CEP63 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The purified fractions were desalted and concentrated using an Amicon Ultra centrifugal filter unit (10-kDa cutoff; Merck Millipore), and the enzyme preparations were stored at −80°C ...
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bioRxiv - Biochemistry 2022Quote: ... The LE11-27 DNA was dialyzed against the same buffer and concentrated to ~ 2.5 mM (Vivaspin 500, 3 kDa MWCO, Merck). The complex was formed by mixing the protein and the DNA in a 2:1 protein-to-DNA ratio (final concentration of 800 µM and 400 µM ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were pooled and concentrated in an Amicon Ultra-4 Ultracell 30 kDa centrifugal filter (Merck-Millipore #UFC803024). Aliquots were snap frozen and stored at −80 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were washed three times in 200 µL of the same buffer in a 30-kDa Amicon Ultra 0.5-mL centrifugal filter (Merck) to remove free ubiquitin ...
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bioRxiv - Bioengineering 2023Quote: ... the medium reservoirs were aspirated and 50 μL EGM-MV2 containing 100 ng/mL 10 kDa FITC-dextran (Merck) added to a single reservoir on each side of the device ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions between 15.7 mL to 19.2 mL in elution volume were concentrated with Amicon Ultra-4 Centrifugal Filter Unit (10 kDa cutoff, Merck) to ∼2 mg/mL (measured by Bradford method) ...
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bioRxiv - Biochemistry 2023Quote: ... concentration and buffer exchange to TE (10 mM Tris-HCl pH7.5 and 1 mM EDTA) with an Amicon Ultra-4 centrifuge filter unit42 (30 kDa cut-off, Merck). Concentration of all plasmids was measured by NanoDrop One (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was transferred to an Amicon Ultra-0.5 Centrifugal Filter Unit 3 kDa (Merck Millipore, Catalogue no. UFC500396) and centrifuged at 10,000 g for 20 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was concentrated to 10% of the initial volume using a 100 kDa cutoff Amicon filter (Merck Millipore).
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bioRxiv - Biochemistry 2023Quote: ... at 4°C (repeated for 10 cycles) to remove salt using an Amicon Ultra-0.5 Centrifugal 10 kDa filter unit (Merck), before dilution to 5.00 µM in 20 mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant was filtered through Amicon® Ultra – 0.5 mL 10-kDa cutoff centrifugal filters (Merck KgaA; Darmstadt, Germany). To test the enzymatic efficiency of DisA before synthesizing [32P]c-di-AMP ...
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bioRxiv - Immunology 2024Quote: ... Cell supernatant was then concentrated by Tangential Flow Filtration with a Pellicon 3 Ultracel 10 kDa membrane (Merck Millipore) and loaded onto a 10 mL CaptureSelect™ C-tagXL affinity column that had been equilibrated in Tris-buffered saline (TBS ...
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bioRxiv - Neuroscience 2024Quote: ... concentrated using 100-kDa molecular weight cut-off (MWCO) filters Centricon plus-20 and Centricon plus-2 (Merck-Millipore), aliquoted and stored at - 80°C ...
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bioRxiv - Biochemistry 2024Quote: ... Dissolved IntC-UbcH5c-CAET-Ub was transferred to a dialyzer (D-Tube Dialyzer Maxi, MWCO 6– 8 kDa, Merck) in 300 mL of unfolding buffer ...
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bioRxiv - Bioengineering 2024Quote: ... AAV was filter-dialyzed with 1X PBS containing 0.001% Tween-20 using an Amicon Ultra 100 kDa MWCO column (Merck). The titer of the purified AAV was determined ...
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bioRxiv - Cell Biology 2023Quote: ... Protease inhibitor was added to sample and sample was concentrated down to 150 µL using Amicon Ultra-0.5 10 kDa Centrifugal Filter Units (Merck). On the other hand ...
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bioRxiv - Biochemistry 2024Quote: ... Purified MPP8 CTD was concentrated to 4.5 mg/ml using a 10-kDa cutoff centrifugal filtration device (Merck Millipore) and mixed with an equal volume (up to 0.5 µl ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue homogenisation and western blotting was performed with 50 μg of total protein as previously described.15 Membranes were incubated overnight with gentle agitation at 4 °C with the MSH3 antibody (Merck MSH3MAB(1F6)) in 5% milk PSB-T (phosphate buffer saline (PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... Thirty µl of Protein G (Protein G Sepharose Fast Flow, Merck, UK) slurry per sample was added in addition to 0.01% sodium azide and incubated on a roller overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Total protein concentration was quantified by means of BCA protein assay (Merck). 10-20 μg of protein was separated by size using a 4-12% Bis-Tris Plus gel ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 µg/ml.
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bioRxiv - Biophysics 2021Quote: ... fluorescently-labeled and biotinylated H57-scFV were concentrated to 0.2 - 1 mg/mL with 10 kDa Amicon®Ultra-4 centrifugal filters (Merck) and stored in 1x PBS supplemented with 50 % glycerol at -20°C ...
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bioRxiv - Biophysics 2021Quote: ... The solution was concentrated with an Amicon Ultra-15 Centrifugal Filter (NMWL of 10 kDa, Merck Millipore, Burlington, MA, USA) and loaded into a Superdex75 10/300 GL gel filtration column (GE Health-care Life Sciences ...
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bioRxiv - Molecular Biology 2021Quote: ... The sample (0.5-1 ml) was transferred to Amicon Ultra 0.5 ml Centrifugal Filters (MWCO=30 kDa, Merck, cat# UFC503096) and further concentrated to a final volume of 200 μl.
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bioRxiv - Molecular Biology 2019Quote: ... the remaining ultracentrifuged supernatant was concentrated to approximately 220 µL using Amicon Ultra-2 10 kDa molecular weight cutoff filters (Merck KGaA ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1 M Tris pH 8.5) and processed according to a CHAPS-based FASP protocol in centrifugal filter units (30 kDa MWCO, Merck Millipore). After removal of the detergents ...
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bioRxiv - Molecular Biology 2021Quote: ... The 40S- and 60S-subunit fractions were mixed and concentrated using Amicon Ultra filter units (MWCO 50 kDa, Merck Millipore). The concentrated mixture was loaded onto a 10–50% (w/v ...
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bioRxiv - Biochemistry 2019Quote: ... On the next day the peptide and GFP were subject to concentration via ultrafiltration (Amicon Ultra 10 kDa MWCO, Merck) and removal of the GFP and 3C protease ...
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bioRxiv - Biophysics 2019Quote: ... The excess monomeric species was removed by multiple filtration using a 100 kDa cutoff centricon (Amicon, Merck Millipore, MA, USA) to enrich the population of the oligomeric species ...
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bioRxiv - Biophysics 2019Quote: ... fractions containing monomeric PSCFP2*-AVI-3C-12xHis were concentrated using Amicon Ultra-4 centrifugal filters (10 kDa cut-off, Merck) in the presence of 100 µM Tris(2-carboxyethyl)phosphine (TCEP ...
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bioRxiv - Biochemistry 2021Quote: ... 100 mM EDTA or 200 mM Imidazole) and concentrated up to 0.5 ml using Amicon Ultra-15 centrifugal filters with MWCO of 50 kDa (Merck, Germany). The sample was then applied to a size exclusion chromatography on a Superdex 200 increase 10 /300GL column equilibrated with wash buffer ...
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bioRxiv - Biophysics 2021Quote: ... The eluted sample was exchanged into 20 mM Tris buffer and concentrated to 5 ml by Amicon Ultra-15 3 kDa (Merck). The sample was purified by 320 ml of HiLoad Superdex with a flow rate of 1 ml/min using the FPLC systems.
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bioRxiv - Cell Biology 2021Quote: ... Soluble samples were desalted and concentrated using Amicon concentrators (10-kDa cut-off; Millipore Sigma, # UFC800308; Merck KGaA, Darmstadt, Germany). Samples (20 µg protein ...
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bioRxiv - Biochemistry 2020Quote: ... each N-Cdh sample was desalted against PBS using Amicon centrifugal filters with a 3 kDa molecular weight cutoff according to the manufacturer’s protocol (Merck-Millipore). Unprocessed xCGE-LIG N-glycocomics raw data are available upon request.
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bioRxiv - Synthetic Biology 2021Quote: ... 100 μL aliquots were removed and the reaction was stopped using a 3 KDa nominal molecular weight limit (NMWL) centrifuge filter (Merck Amicon Ultra 0.5mL Centrifugal Filters ...
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bioRxiv - Neuroscience 2021Quote: ... then dialysed against the same stock of ITC buffer overnight at 4°C using 1 kDa Pur-a-lyzer tubes (Merck). Protein and RNA concentrations after dialysis were calculated by A280 and A260 absorbance respectively ...
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bioRxiv - Molecular Biology 2019Quote: ... The resulting glycoconjugate was washed and concentrated with deionized water using 10 kDa centrifugal filter units (Merck Millipore, Tullagreen, Ireland).
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bioRxiv - Microbiology 2020Quote: ... The eluate was neutralised immediately using 1.5 M Tris pH 8.6 and then buffer exchanged to PBS using a 15 ml 30 kDa MWCO centrifugal filter (Merck Millipore).
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bioRxiv - Cancer Biology 2021Quote: Urine samples were first adjusted to pH 8.0 using 500 mM ABC supplemented with EDTA-free protease inhibitor and then filtered through an Amicon Ultra-0.5 mL Centrifugal Filter Device (10 kDa, Merck Millipore). Collection of the retentate was performed by flipping the filter device upside-down and conducting centrifugation at 1,000g for 2 min ...
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bioRxiv - Biochemistry 2022Quote: ... Purified ShTniQ was concentrated to 10 mg mL−1 using 10,000 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1 M Tris pH 8.5) and processed according to a CHAPS-based FASP protocol in centrifugal filter units (30 kDa MWCO, Merck Millipore). After removal of the detergents ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions that were highly enriched for Redβ177 were pooled and concentrated in a 10 kDa MWCO centrifugal concentrator (Merck Millipore) to a total volume of <2 ml ...
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bioRxiv - Biochemistry 2022Quote: ... Elution fractions containing MbxA were pooled and concentrated with Amicon Ultra-15 Centrifugal Filter Units (50 kDa NMWL, Merck Millipore). Histidine was removed with a PD10 desalting column (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining media was then concentrated down to 500 uL using a falcon tube sized 3 kDa amicon column (UFC900308; Merck) spun at 4000 xg and 4°C for approximately 1 h ...
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bioRxiv - Biophysics 2022Quote: ... The elution volume of 60-70 mL was collected and the solution was concentrated to a volume of 200 μL with an Amicon Ultra-15 PLHK Ultracel-PL Membrane 100 kDa (Merck) spin column ...
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bioRxiv - Biochemistry 2022Quote: ... Purified ShTnsC was concentrated to 1-2 mg mL−1 using 30,000 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Bioengineering 2022Quote: ... 100 nM of RNA polyhedra samples folded in TE/Mg2+/Na+ buffer were concentrated four times using 3 kDa MWCO Amicon Ultra centrifugal filters (Merck). 5 µl of the concentrated sample was applied on 300 mesh Cu grids coated with lacey carbon (Agar Scientific) ...