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Citations for Merck :
501 - 550 of 2598 citations for Centrosomal Protein 63 kDa CEP63 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... the LUVs were transferred to an Amicon Ultra-4 100 kDa centrifugal filter unit (Merck, Darmstadt, Germany) and concentrated by centrifuging at 2000g ...
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bioRxiv - Bioengineering 2023Quote: ... ZIKV stocks were also column concentrated using Amicon™ 100 kDa Ultra-15 Centrifugal Filter Units (Merck).
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bioRxiv - Molecular Biology 2022Quote: ... concentrated with an Amicon ultra-4 centrifugation filter with a 10 kDa molecular weight cutoff (Merck Millipore), snap frozen and stored in -80 °C either directly (used for cryo-EM ...
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bioRxiv - Neuroscience 2023Quote: ... AAV suspension was concentrated using centrifugal devices (Amicon® Ultra-15 100 kDa centrifugal filter devices, Merck) and resuspended in PBS with 0.001% Pluronic-F68 before storage ...
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bioRxiv - Neuroscience 2023Quote: ... Purified pooled EVs were further concentrated by ultrafiltration using Amicon Ultra 100 KDa Centrifugal Filters (Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... unreacted biotin-pentylamine was removed by ultrafiltration with a 3 or 10 kDa MWCO membrane (Merck Millipore). The recovered upper residual liquid (∼100 μL ...
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bioRxiv - Neuroscience 2023Quote: ... Viral particles solution was collected and ultrafiltered with Amicon Ultra-15 centrifugal filters (100 kDa, Merck, Germany) to obtain the pure AAVs solution for animal administration.
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bioRxiv - Biochemistry 2024Quote: ... The buffer was then exchanged using an Amicon Ultra-0.5 centrifugal filter unit 50 kDa (Merck KGaA). FtsZ purity was checked by SDS-polyacrylamide gel electrophoresis and concentration was determined by absorbance at 280 nm using an extinction coefficient (ε ...
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bioRxiv - Genetics 2021Quote: ... Prior to EMSAs the in vitro translations of Rbpj (WT) and mutant proteins were monitored by western blotting using an anti-Flag antibody (M5, Merck).
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore ...
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bioRxiv - Microbiology 2022Quote: ... The clear cell-free extracts were immunoprecipitated using monoclonal antibodies against T18 tag following Protein G Immunoprecipitation Kit (Merck Inc) protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The amount of puromycin incorporated into nascent peptides was then evaluated by Western blot analysis on 20 μg of proteins using anti-puromycin antibody (ref MABE343) purchased from Merck Millipore (Darmstadt ...
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bioRxiv - Cell Biology 2023Quote: ... The amount of puromycin incorporated into nascent peptides was then evaluated by Western blot analysis on 20 μg of proteins using anti-puromycin antibody (ref MABE343) purchased from Merck Millipore (Darmstadt ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitations were performed as previously described7 using Protein G Dynabeads (LifeTechnologies) coupled to Myc 4A6 antibody (Merck Millipore, 05-724) or FLAG M2 monoclonal antibody (Sigma ...
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bioRxiv - Biophysics 2021Quote: ... refolded H57-scFV were concentrated using Amicon®Ultra-15 centrifugal filters with a 10 kDa cutoff (Merck) and purified via gel filtration using Superdex 200 (10/300 ...
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bioRxiv - Biochemistry 2020Quote: ... and relevant fractions were pooled and concentrated using a 30 kDa molecular weight cut-off centrifugal concentrator (Merck). The his-tag was removed by incubating concentrated protein overnight with Tobacco Etch Virus (TEV ...
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bioRxiv - Cell Biology 2019Quote: ... TCEP was removed via 10 kDa molecular weight cut-off (MWCO) Amicon spin filters (Merck, Cat. No. UFC500324) and the nanobody was coupled through maleimide conjugation chemistry to a maleimide-DBCO crosslinker (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... purified ParB was concentrated by centrifugation in an Amicon Ultra-15 3-kDa cut-off spin filters (Merck) before being loaded into a Superdex 75 gel filtration column (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was then filtered through 5-kDa cut-off filters (Ultrafree-MC PLHCC, Merck KGaA, Darmstadt, Germany) to remove macromolecules ...
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bioRxiv - Immunology 2021Quote: ... pH7.9 and concentrated to 10 mg/ml using Amicon Ultra-15 centrifugal filters (10 kDA cutoff, Merck Millipore). The protein concentration was determined by the bicinchoninic acid (BCA ...
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bioRxiv - Biophysics 2019Quote: ... Fractions containing monomeric mSav*-STAR635 were concentrated with Amicon Ultra-4 centrifugal filters (10 kDa cut off, Merck) to arrive at a protein concentration of ~1 mg ml−1 ...
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bioRxiv - Biophysics 2019Quote: ... Fractions containing monomeric PS-CFP2 were concentrated with Amicon Ultra-4 centrifugal filters (10 kDa cut-off, Merck) and the maturation state of the fluorescent PS-CFP2 protein was verified via protein absorbance at 280 nm and 400 nm ...
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bioRxiv - Biophysics 2021Quote: ... for 2 hours at 37°C and purified using an Amicon 30 kDa MWCO filter (Merck, Darmstadt, Germany). The Deep Vent exo- DNA polymerase (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... purified ParBΔCTD was concentrated by centrifugation in an Amicon Ultra-15 3-kDa cut-off spin filters (Merck) before being loaded into a Superdex-200 gel filtration column (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... Eluted fractions containing monomeric H57-scFV were concentrated with 10 kDa Amicon® Ultra-4 centrifugal filters (MERCK), treated with a 3C protease (71493 ...
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bioRxiv - Physiology 2020Quote: ... at 4°C for 60 min using an Amicon Ultra-4 3 kDa centrifugal filter device (Merck Millipore). The 50 μL retentate was the final volume of concentrated EVs ...
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bioRxiv - Microbiology 2021Quote: ... This crude enzyme solution was desalted using ultra centrifugal filters of 10 kDa cutoff value (Amicon Ultra, Merck) and then loaded on a 15 mm× 200 mm DEAE cellulose column pre-equilibrated with 20 mM Tris-Cl buffer (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EGTA and protease inhibitors) on an Amicon 30 kDa cut off concentrator (Merck, Cat# UFC 503096). Protein concentration was determined using a BCA protein assay (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 8 M urea and transferred onto a 30 kDa molecular weight cut-off column (Microcon-30, Merck Millipore). 150 μL 100 mM TRIS/HCl pH 8.5 ...
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bioRxiv - Biophysics 2022Quote: ... for 2 hours at 37°C and purified using an Amicon 30 kDa MWCO filter (Merck, Darmstadt, Germany). Deep Vent exo-DNA polymerase (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... Strep-Tactin purified mPSF complexes were concentrated in centrifugal filter (Amicon Ultra-15, MWCO 300 kDa, Merck Millipore) to approximately 0.5 mg·ml-1 ...
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bioRxiv - Biophysics 2022Quote: ... using an Amicon® Ultra (molecular weight cut-off 30 kDa) centrifugal filter device (Merck Millipore, Darmstadt, Germany). Samples were kept frozen at -80 °C for future use.
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bioRxiv - Systems Biology 2022Quote: ... 70 kDa FITC-dextran and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were purchased from Merck Life Science UK Ltd. ...
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rRNA methylation by Spb1 regulates the GTPase activity of Nog2 during 60S ribosomal subunit assemblybioRxiv - Biophysics 2022Quote: ... Eluted pre-ribosomes were concentrated on Amicon Ultra 0.5ml spin columns with a 100 kDa cutoff (Merck Millipore). Starter strains carrying spb1D52A were grown for 36 h to reach saturation and 12L cultures were grown to 12 – 16 hours to reach an OD600 of 0.8 ...
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bioRxiv - Biophysics 2022Quote: Purified FHL was concentrated to 1-2 mg/mL using an Amicon centrifugal concentrator (100-kDa cutoff; Merck) and 3 µL of protein solution was applied to freshly glow-discharged C-flat 1.2/1.3 or 2/1 holey carbon grids (Protochips) ...
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bioRxiv - Biophysics 2022Quote: ... The solution was concentrated by ultrafiltration using Amicon Ultra centrifugal filters with 100 kDa molecular weight cutoff (Merck) and passed over a Superdex 200 26/60 gel filtration column (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatant was collected and concentrated with Amicon Ultra centrifugal filter devices with 10 kDa cut-off membrane (Merck). Protein concentration was measured by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... and relevant fractions were pooled and concentrated using a 10 kDa molecular weight cut-off centrifugal concentrator (Merck). The his-tag was removed by incubating concentrated protein overnight with Tobacco Etch Virus (TEV ...
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bioRxiv - Microbiology 2020Quote: ... The eluted fractions were concentrated in a 10 kDa cut-off ultra-filtration device (Amicon Ultra, Merck Millipore) by centrifugation at 4,000 x g to ∼200 μl ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 8.5 and centrifuged at 14,000 g for 20 minutes using a 30 kDa mass-cutoff 0.5 ml Amicon centrifugal filter (Merck). For alkylation of cysteines ...
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bioRxiv - Biochemistry 2021Quote: ... the VmeCmr complex-containing flow-through was concentrated using an Amicon Ultracentrifugal filter (30 kDa MWCO, Merck-Millipore) and further purified by size exclusion chromatography (HiPrep™ 16/60 Sephacryl® pg 300 HR ...
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bioRxiv - Cell Biology 2021Quote: ... and 3ml of medium was concentrated to 50-100μl in Microcon - 10 kDa centrifugal filter units (Merck Millipore). Samples were run on 10% Bis-Tris gels (Thermo #NW00100BOX ...
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bioRxiv - Genomics 2021Quote: ... and concentrated using centrifugal filters having a 10 kDa molecular weight cut off (MWCO) (Merck KGaA, Darmstadt, Germany). The purified DNA-barcoded lectin was analyzed by SDS-PAGE ...
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bioRxiv - Biophysics 2022Quote: ... The eluent was concentrated using an Amicon Ultra-15 100-kDa cut-off centrifugal filter unit (Merck Millipore) before purification on a Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... were concentrated to 5 mL by 10 kDa Amicon® Ultra Centrifugal Filters (Lot No. R9EA18187, Merck Millipore) and then applied for CsCl density gradient centrifugation at 24,000 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... and concentrated at 4 °C approximately 300 times using a 10 kDa Centricon Plus-70 centrifugal unit (Merck Millipore ...
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bioRxiv - Immunology 2023Quote: ... Fractions containing monomeric and labeled 14.4.4 scFVs were concentrated with Amicon Ultra-4 centrifugal filters (10 kDa cut off, Merck) to arrive at a protein concentration between 0.2 mg ml-1 and 1 mg ml-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... pooled according to purity and afterwards concentrated using Amicon Ultra-4 centrifuge filters (30 kDa cutoff, Merck Millipore). The purified octamers were validated by SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: Supernatants showing biological activity were concentrated using Amicon Ultra-0.5 centrifugal filters (3 kDa) (Merck KGaA, Darmstadt, Germany) as follows ...