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Citations for Merck :
301 - 350 of 1195 citations for 7 8 Dihydroisoquinolin 5 6H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 5 ng/ml EGF (Merck, cat. #SRP3196), 50 nM dexamethasone (Merck ...
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bioRxiv - Microbiology 2024Quote: ... 5% glycerol) supplemented with Benzonase® (Merck) and cOmplete™ Mini EDTA-free protease inhibitor (Roche) ...
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bioRxiv - Plant Biology 2020Quote: ... samples were resuspended in Urea 7 M and Thiourea 2 M buffer and desalted on Amicon Ultra-0.5 3 kDa centrifugal filters (Merck Millipore, Germany). Filters were filled to maximum capacity with buffers and centrifuged at 15,000 g for 10 min at 20°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and additional wells with 20% biotinylated PLL-g-PEG and FNIII(7-10) were supplemented with 1 µM staurosporine (Merck Millipore) to serve as a positive control for apoptosis ...
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bioRxiv - Cancer Biology 2022Quote: ... The purified protein was dialyzed overnight in 25 mM Tris-Cl pH 7.5 using SnakeSkin®ialysis tubing (7 kDa molecular weight cutoff) and concentrated using Amicon™ Ultra-15 Centrifugal Filter Units (30 kDa molecular weight cutoff) (Merck) according to the requirements of the experiments.
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bioRxiv - Microbiology 2022Quote: A CRISPR array containing 6 identical spacers targeting the tetR gene flanked by 7 repeats was cloned into pCDF-Duet (Novagen, Merck Millipore) by ligation after NcoI and SalI digestion ...
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bioRxiv - Microbiology 2023Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with polyclonal rabbit anti-TipR antibodies (1:400 dilution) (Kirkpatrick and Viollier, 2014) or monoclonal rabbit anti-HA antibodies (1:250 dilution) (Clone 114-2C-7, Merck Millipore). ...
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bioRxiv - Neuroscience 2024Quote: ... for 5 x 7 min and incubated 2h at room temperature (RT) with blocking solution containing 10% normal donkey serum (NDS, Merck Millipore), 3% Bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: ... The digested suspension was pelleted and the pepM extract buffer exchanged to PBS pH 7 using 10 kDa Amicon centrifugal filter unit (Merck, Ireland). PepM extracts were confirmed with SDS-PAGE and stored in solution at − 20°C.
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bioRxiv - Microbiology 2023Quote: ... The flow-through was sterile filtered and concentrated to a final volume of 7 ml using Amicon Ultra-15 Centrifugal filter concentrator (Merck Millipore).
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bioRxiv - Microbiology 2021Quote: ... One mL of virus stock and 9 mL of MEM-E supplemented with 1% pyruvate (Merck KGaA, Darmstadt, Germany) were added to a conical tube with 16 × 106 SK-6 cells and shaken for 30 minutes at 104 rpm and 37°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... One milliliter of the virus supernatants was directly overlaid on the cells in the presence of polybrene (Merck, Germany) at a final concentration of 4 μg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin, Bovine Serum, 12659, Merck Millipore) powder solved in 1 × TBST ...
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bioRxiv - Microbiology 2019Quote: ... The pellet was dissolved in three ul distilled water and spotted onto 5×5 cm silica gel plates (Merck) using automatic TLC sampler ...
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bioRxiv - Developmental Biology 2021Quote: ... The HPLC was equipped with a hydrophilic ZIC-pHILIC column (150 × 2.1 mm, 5 μm) with a ZIC-pHILIC guard column (20 × 2.1 mm, 5 μm, Merck Sequant). 5ul of each sample was used for each assay ...
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bioRxiv - Cell Biology 2021Quote: ... cells were starved overnight in serum-free DMEM and then treated for 1 hour with 5 ng/ml TGFβ1 (Preprotech) or with 5 μM SB431542 (Merck), and intensities quantified by ImageJ ...
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bioRxiv - Microbiology 2022Quote: ... were washed with RPMI 2%G by applying 5 psi perfusion for 5 min using the CellASIC® ONIX2 microfluidic system (version 1.0.4 Millipore Merck). Yeasts were loaded into the CellASIC culture chambers by applying 8 psi for 5 s twice (Thomson et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... mature pollen was germinated on the surface of solid PGM (18% sucrose, 0.01% H3BO3, 5 mM CaCl2, 5 mM KCl, 1 mM MgSO4 (Merck), pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were incubated with Prussian’s blue solution containing ferrocyanide acid solutions (5% Hydrochloric acid (HCl) and 5% potassium ferrocyanide (Merck)) at a 1:1 ratio for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5 ml conditioned medium was centrifuged (5 min, 500g) and filtered through Amicon Ultra-0.5 Centrifugal Filter unit (Merck). Ammonia in TIFs and SCFs was then quantified using Dimension Ammonia assay (Siemens ...
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bioRxiv - Microbiology 2019Quote: ... Each sample was separated on 8% polyacrylamide gels and transferred onto 0.45 µm PVDF membranes (Merck, Darmstadt, Germany). PVDF membranes were blocked with PBS containing 0.05% Tween 20 (PBST ...
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bioRxiv - Physiology 2019Quote: Chemotaxis of hMSCs was assessed using Boyden chambers with a pore size of 8 μm (Merck Millipore, PIEP12R48). Cells were seeded on the upper membrane in serum free α-MEM medium at a density of 30,000 cells/cm2 and allowed to adhere 4 h before being transferred to the wells containing chemotactant (Medium (serum free) ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated by 8-12% SDS-PAGE and transferred to PVDF membranes (Millipore-Merck). Membranes were incubated with primary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... 8 M urea and transferred onto a 30 kDa molecular weight cut-off column (Microcon-30, Merck Millipore). 150 μL 100 mM TRIS/HCl pH 8.5 ...
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bioRxiv - Developmental Biology 2019Quote: ... The viral supernatant was mixed with 8 μg/ml DEAE-dextran and 1000 units/ml ESGRO (Merck Millipore) and added directly to the E14tg2a cells ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The protein samples were separated by 8% and 12% SDS PAGE and were transferred to PVDF membranes (Merck Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... thawed pellet was resuspended into 20 ml lysis buffer (0.025 M Tris pH 8; 0.5 M NaCl; 2 mM MgCl2; 100 U/ml Benzonase (Merck); 0.25 mg/ml lysozyme (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... before transduction of TREx BCBL1-Rta cells in the presence of 8 μg/mL of polybrene (Merck Millipore). Virus supernatant was removed 6 hours post transduction and fresh media added ...
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bioRxiv - Neuroscience 2022Quote: ... as internal standard (IS) in 67% deionized water and 33% acetonitrile (ACN, 900667, CAS 75-05-8, Merck). The LTQ Orbitrap ELITE ETD (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... ECD peak fractions were collected from pH 8 experiments and buffer exchanged using centrifugal concentration units (Amicon, Merck) into pH 6 purification buffer (50 mM MES ...
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Preference of CAMSAP3 for expanded microtubule lattice contributes to stabilization of the minus endbioRxiv - Biophysics 2023Quote: ... pH 7.3) supplemented with 8% glycerol and 0.5 mM DTT using a 30-kDa-cutoff centrifugal filter (UFC503096; Merck). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclear translocation of FLAG-ER-SAMD1 was induced by adding 200 nM 4-OHT (Merck; 68392-35-8) for 24 h.
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bioRxiv - Cancer Biology 2022Quote: ... cells were transduced with 1 MOI of concentrated virus containing 8 ug/mL Polybrene (Merck, TR-1003-G). Two days later ...
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bioRxiv - Biochemistry 2023Quote: ... pH 8) and concentrated to 30 mg/ml using an Amicon Ultra-15 Centricon (Millipore Merck, Darmstadt, Germany). Crystallization experiments were performed with ORYX8 pipetting robot (Douglas Instruments ...
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The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretionbioRxiv - Neuroscience 2024Quote: ... They were then incubated with rabbit anti-cholecystokinin (26–33) (CCK-8) antibody (diluted 1:1000, Merck, C2581) for 16 h at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... 5.5 µL of enzyme solution (275 ng of Trypsin Gold, Promega V5280; and 8 units of Benzonase, Merck Millipore 70746 ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 2 × 104 cells were cultured in an 8-well chamber slide (Merck Millipore, Massachusetts, USA) and then fixed with 4% paraformaldehyde in PBS buffer for 15 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Proteins in the serum were removed by passing it through a pre-rinsed (7 times washed) Amicon Ultra-2ml 3000 MWCO (Merck Millipore, USA) column ...
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bioRxiv - Neuroscience 2020Quote: ... The cortices were homogenized mechanically in PBS by pipetting up and down with a 5mL plastic pipette, centrifuged (500 x g, 7 min, 4 °C) and resuspended in DMEM (Sigma-Aldrich, Merck, Switzerland) containing 5% FCS (Biochrom ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... 50 µL benzaldehyde (BA) and 250 µL 3-octanol (OCT) (CAS 100-52-7, and 589-98-0, respectively; Merck, Darmstadt, Germany) were applied undiluted to 1-cm-deep Teflon containers of 5 and 14 mm diameter ...
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bioRxiv - Molecular Biology 2021Quote: ... the protocol described by Choi et al.65 was applied using home-made wash buffer (50% formamide (Merck Millipore, 75-12-7), 5XSSC (Molecular Biology-P ...
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bioRxiv - Microbiology 2022Quote: ... Five duplicate plates were spread individually by six gradient diluted soil suspensions from 10−2 to 10−7 dilution on 1/10 TSA medium (Tryptic Soy Agar, Merck, Darmstadt, Germany) and incubated under both aerobic and anaerobic conditions at 28 °C for two weeks ...
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bioRxiv - Genomics 2023Quote: ... These assessments were conducted on 7-day old Foc strains cultured on petri dishes with potato dextrose agar (PDA; Merck, Darmstadt, Germany). An approximately 5 mm piece of the actively growing colony from each isolate was placed on PDA plates and incubated at 27°C ...
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bioRxiv - Neuroscience 2023Quote: ... Flies were raised at 25℃ and collected on the day of eclosion and kept in the dark for 7-10 days on the fly media containing 0.4 mM all trans-Retinal (Merck, St. Louis, MO). The experiments were performed with the “8-well” chamber (16 mm diameter x 10 mm height ...
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bioRxiv - Cancer Biology 2024Quote: ... Two sets of four EV-rich fractions (7–12 and 16-22) were pooled and concentrated using Amicon Ultra-4 10 kDa centrifugal filter device (Merck Millipore, USA). Alternatively ...
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bioRxiv - Bioengineering 2021Quote: ... hiPSCs were transduced with LV particles at MOI of 5 in the presence of 5 µg/mL of polybrene (Merck). Stably transduced cells were obtained upon selection with 0.3 µg/mL of puromycin for 6 days ...
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bioRxiv - Biochemistry 2020Quote: ... cells were washed with PBS and permeabilized for 5 min with 0.1% Triton X-100 in PBS and blocked with 5% ChemiBlocker (Merck-Millipore) in PBS for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... the pUL21 gene was amplified from virus stock by PCR with the oligonucleotide primers 5′-ATGGAGCTTAGCTACGCCAC-3′ and 5′-TTTATTGGGGTCTTTTACACAGACTGTC-3′ using KOD polymerase (Merck) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 5 µl of standards or samples were injected onto SEQuant ZIC-pHILIC column (Merck, PEEK 150 × 2.1 mm, 5 µm). MS analysis was performed in negative-ion mode over the mass range from 200 to 1,000 m/z ...
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bioRxiv - Cancer Biology 2022Quote: ... and 100 pM of synthetic guide RNA (Tspan8 guide sequence 5’ – 3’: GGGGAGTTCCGTTTACCCAA; Thrsp guide sequence 5’ – 3’: AGTCATGGATCGGTACTCCG; Merck) were mixed and incubated at RT for a minimum of 10min to assemble the ribonucleoprotein (RNP ...