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Citations for Merck :
351 - 400 of 4542 citations for 6 Chloroimidazo 1 2 b pyridazine 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Apoptotic cell death was detected by annexin-V/propidium iodide staining and subsequent flow cytometry analysis using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Annexin-V-FITC emission was detected with the green filter at 525/530 nm ...
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bioRxiv - Immunology 2021Quote: ... was mixed 1:1 and incubated at RT for 2-3 min or ECL substrate is added (Immobilon crescendo western HRP substrate, WBLUR0100, Merck). Membranes were then exposed to film and developed or visualised by chemiluminescence using the G:BOX Chemi gel doc Imaging System Instrument (Syngene) ...
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bioRxiv - Neuroscience 2021Quote: Viability of SH-SY5Y cells after treatments with or without H-LIPEF was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
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bioRxiv - Neuroscience 2020Quote: ... They were cut into 250μm parasagittal slices using a McIlwain tissue chopper and the slices placed on Millicell membrane (3-4 slices per membrane, 2 membranes per animal, 0.4 μm Millicell, Merck Millipore) in 50% BME (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... The purified proteins were concentrated to 2 mg/mL using centrifugal filters with either 3 kDa or 30 kDa cut-off (Amicon Ultra-0.5, Merck/Millipore) and the samples were centrifuged at 100,000 g ...
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bioRxiv - Genetics 2021Quote: ... At day 10 cells were passaged at a 2:3 ratio into 12 well cell culture plates coated with 15 µg/ml human plasma fibronectin (Merck) in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Pathology 2023Quote: ... To prevent unspecific labelling the sections were incubated for 2 × 10 minutes in PB solution containing 3% hydrogen peroxide (Merck Life Science AS/Sigma Aldrich Norway AS ...
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bioRxiv - Microbiology 2023Quote: ... were pre-prepared by washing 3 times in immunoprecipitation buffer then resuspended in 200μl immunoprecipitation buffer and coated with 2 μl FLAG M2 (Merck Millipore) or IgG (Merck Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was supplemented with calcium chloride to a final concentration of 3 mM and chromatin was solubilised for 2 h at 4 °C by digestion with Turbonuclease (T4330, Merck) to a final concentration of 250U/ml ...
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bioRxiv - Biophysics 2023Quote: ... The C378A or C406A variants of 15N-pm-β2AR-Cter were labeled on the remaining cysteine using 3-(2-Iodoacetamido)-proxyl (Merck). Paramagnetic samples were recorded with a recycling delay of 2 s ...
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bioRxiv - Neuroscience 2023Quote: ... To avoid unspecific labelling the sections were first incubated for 2 × 10 minutes in a solution of PB with 3% hydrogen peroxide (Merck Life Science AS/Sigma Aldrich Norway AS ...
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bioRxiv - Genomics 2023Quote: ... DNA was fragmented in a 2 mL low-bind round bottom Eppendorf tube using a sterile 3 mm borosilicate bead (Z143928-1EA Merck) by vortexing for 1 minute at maximum speed as described in [23] ...
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bioRxiv - Molecular Biology 2024Quote: ... the beads were washed 3 more times with 100 µL lysis buffer 2 supplemented with 100 µg/mL polyU RNA (Merck). The bound complexes were eluted in lysis buffer 2 containing 25 mM glutathione or 5 mM biotin (Merck) ...
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bioRxiv - Neuroscience 2024Quote: ... animals at the age of 3 months were perfused using 2% Formaldehyde (Science Services, München, Germany) and 2.5% Glutaraldehyde (Merck, Darmstadt, Germany) in 0.1M Cacodylate buffer ...
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bioRxiv - Immunology 2024Quote: The viabilities of the PBMCs or U-937 macrophages were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
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bioRxiv - Synthetic Biology 2022Quote: Total bile acids in feces was measured using Bile Acid Assay Kit (Sigma-Aldrich MAK309, Merck, France). The L-lactate concentration was measured using a L-lactate Assay Kit (Sigma-Aldrich MAK329 ...
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bioRxiv - Cell Biology 2024Quote: ... 1 nM β-estradiol and 2 μg/mL insulin (Merck). Any red blood and unattached cells were removed by media change within 18 hours ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... λem□=D525□nm) and l-α-phosphatidylethanolamine-N-(lissamine rhodamine-B sulfonyl) (ammonium salt) (PE-Rh-B) (fluorescent acceptor, λem□=□595░nm) (Merck KGaA, Darmstadt, Germany) in 1:7 DM ratio ...
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bioRxiv - Microbiology 2021Quote: ... 6-hexanediol reagent (Sigma-Merck, 240117) were heated to 45°C and diluted to 50% in water as stock solution ...
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bioRxiv - Bioengineering 2021Quote: ... 6% Ficoll PM-400 (Merck kGaA), 0.2% Sarkosyl (Merck kGaA) ...
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bioRxiv - Cell Biology 2021Quote: ... for up to 6 days (Merck). The medium with the drug was renewed every 48 h.
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bioRxiv - Bioengineering 2021Quote: ... 12 mg of Fast-Violet B Salt (F1631, Merck KGaA), and 2 mL of Naphthol-AS-MX ALP solution (855 ...
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bioRxiv - Genomics 2019Quote: ... were pulse-labelled with 50 μM BrdU (Merck, B-5002) prior to recovery ...
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bioRxiv - Bioengineering 2021Quote: ... Part B was made of 10U/mL human thrombin (Merck) in DMEM/F12 ...
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bioRxiv - Microbiology 2019Quote: ... and solvent B (100% acetonitrile, Hypergrade for LCMS LiChrosolv, Merck) at a flow rate of 0.3 mL/min ...
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bioRxiv - Neuroscience 2021Quote: ... and it was neutralised with Polymyxin B (Merck, Darmstadt, Germany). Blocking experiments used the anti-ISRAA antibody (1) ...
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bioRxiv - Cancer Biology 2021Quote: ... 1x B-27 (Thermo) and 0.4ug/ml hydrocortisone (Merck, Sigma) along with 1.25% geltrex (Thermo ...
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bioRxiv - Genetics 2022Quote: ... Plates were coated with 0.1% gelatin (Merck, #ES-006-B) and incubated at 37°C and 5% CO2 for 30 min before plating ...
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bioRxiv - Microbiology 2023Quote: ... in the presence of 1.25μg/ml polymyxin B (Merck Millipore) (see also supplementary Figure S2) ...
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bioRxiv - Immunology 2023Quote: ... Staphylococcus-Enterotoxin-B (SEB) (5 µg/ml, Merck Sigma-Aldrich), DAPI (Merck Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Two solutions (”A” and ”B”) of Epon-812 (45345, Merck) were made with different hardeners ...
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bioRxiv - Cancer Biology 2024Quote: ... 25ng/mL cholera toxin subunit B (Sigma/Merck, C9903-.5MG), 0.5µg/mL hydrocortisone (Sigma/Merck ...
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bioRxiv - Cell Biology 2023Quote: ... for 10 min and with 0.1% Sudan black B (Merck) in 70% ethanol for 20 min to quench the autofluorescence of immune cells ...
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bioRxiv - Biochemistry 2019Quote: ... and csm5 were cloned into pACYCDuet-1 (5’-NcoI, 3’-XhoI; Novagen, Merck Millipore), csm4 was cloned into pEHisTEV (5’-NcoI ...
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bioRxiv - Neuroscience 2019Quote: ... further immunolabeling was used either against Vesicular Glutamate Transporter 3 (1:2000, VGluT3; Merck, Cat#AB-5421 ...
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bioRxiv - Molecular Biology 2022Quote: ... faecalis was diluted 1:100 into 3 L of Brain heart infusion (BHI, Merck) and grown at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... 1 mM DTT) and concentrated using a 3 kDa MWCO Centriprep concentrators (Merck Millipore). For best performance in the iSAT reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... integrity was inspected on 1% agarose gel in TAE buffer pre-stained with GelRed nucleic acid stain (Merck).
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bioRxiv - Molecular Biology 2021Quote: ... 3% casein (Merck) in 20 mM TBS (pH 11.0) ...
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bioRxiv - Cancer Biology 2023Quote: ... Nutlin-3 (Merck) was dissolved in DMSO and diluted in saline.
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P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: ... The cells were then stimulated with 100μM 2’(3’)-O-(4-Benzoylbenzoyl) adenosine-5’-triphosphate (BzATP; Merck Life Sciences, Gillingham, UK) to activate the P2RX7 ...
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bioRxiv - Biophysics 2019Quote: ... Fragments (2 mM in DMSO) were injected (2 μL) onto a Purospher STAR RP-18 end-capped column (3 μm, 30 × 4 mm, Merck KGaA). Chromatographic separation was carried out over a 4-min gradient elution (90:10 to 10:90 water:methanol ...
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bioRxiv - Plant Biology 2020Quote: ... samples were resuspended in Urea 7 M and Thiourea 2 M buffer and desalted on Amicon Ultra-0.5 3 kDa centrifugal filters (Merck Millipore, Germany). Filters were filled to maximum capacity with buffers and centrifuged at 15,000 g for 10 min at 20°C ...
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bioRxiv - Plant Biology 2023Quote: ... and incubated at 28°C shaking at 200 rpm for 2–3 h before plating on LB agar supplemented with 50 µg/ml Kanamycin (Merck Millipore). The plates were incubated for 2– 3 days at 28°C ...
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bioRxiv - Microbiology 2023Quote: MDCK cells (2 x 105 cells) were cultured on polycarbonate Millicell culture plate inserts (12- mm diameter, 3-µm pore size; Merck Millipore) at 37°C under 5% CO2 atmosphere.
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bioRxiv - Plant Biology 2024Quote: ... samples were acidified to pH 2 with formic acid and desalted with pipette tips packed with six layers of C18 Empore™ SPE Disks (Merck, cat. no.66883-U) and dried in a Speed Vac at 45 °C for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... and formic acid were purchased from Merck Millipore (Darmstadt ...
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bioRxiv - Biochemistry 2019Quote: Glacial acetic acid (Merck, cat. no. 100066)
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bioRxiv - Bioengineering 2021Quote: ... with 5 mM sulfuric acid (>99.5 %, Merck) as a mobile phase at 45 °C for ethanol ...
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bioRxiv - Pathology 2022Quote: ... Periodic Acid-Schiff staining (PAS, Merck, 101646) and Masson’s trichrome staining (IHC world ...