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Citations for Merck :
4801 - 4850 of 5744 citations for Trans Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%;1 D 97% 97% Chemical Purity since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... One sample was incubated with 50 µg/ml TPCK-treated Trypsin (Merck) in 1x PBS at 37°C for 30 min while the other sample (control ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Supernatants were loaded to HybridSPE-Phospholipid cartridges (500 mg/6 mL, Merck) prewashed with 12 mL MeOH and 12 mL ACN containing 0.5% CA ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended ∼2x pellet volume extraction buffer (30 mM HEPES-NaOH pH = 7.8, 100 mM NaCl, 0.5% NP-40, 20 mM iodoacetamide (Merck, 407710), cOmplete™ ...
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bioRxiv - Molecular Biology 2020Quote: ... cell pellets were resuspended in 100 μl Lysis Buffer A, incubated (37°C, 20 min) with 0.5 μl Benzonase (Merck), and boiled (5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were resuspended in ice cold PBS containing 0.1 % Triton X-100 and protease inhibitor cocktail (Merck, 4693116001), in order to extract the cytoplasm ...
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bioRxiv - Microbiology 2021Quote: ... Filtered samples were centrifuged at 5000G using an Amicon ® Ultra 15 (100 kDa) (Merck Millipore, Tullagreen, Cork, IRL) following the manufacturer’s protocol (30 min of centrifugation for every 12 ml) ...
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bioRxiv - Bioengineering 2021Quote: ... all solutions were purified using Amicon centrifugal ultra-filtration devices (Amicon Ultra-2, Sigma Aldrich, 100 kDa membrane, Merck). Prior to use ...
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bioRxiv - Biophysics 2021Quote: ... the culture supernatant was collected and concentrated using the Amicon Ultra centrifugal filter unit with 100 kDa membrane (Merck), aliquoted and stored in -80°C.
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bioRxiv - Biophysics 2021Quote: ... The culture supernatant was collected after 72 h and concentrated using Amicon Ultra-15 MWCO 100 kDa (Merck Millipore).
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bioRxiv - Plant Biology 2021Quote: ... NaOCl and 0.02% (v/v) Triton X-100 for 10 min and washed five times with ultrapure water (Milli-Q; Merck). Following seed stratification at 4°C for 2 d ...
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bioRxiv - Microbiology 2020Quote: ... 100 μl of each of the standardised bacterial test suspension was spread on the Mueller-Hinton agar (Merck, Johannesburg) plates using sterile cotton swabs ...
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bioRxiv - Microbiology 2021Quote: Cells grown on glass coverslips were fixed in 4% PFA for 30 min at RT and permeabilized with 0.2% Triton X-100 (Sigma/Merck) in PBS for 10 min at RT ...
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bioRxiv - Biophysics 2022Quote: ... Unconjugated streptavidin and Dpeg4-biotin were removed via ultrafiltration (Amicon Ultra-0.5, 100 kDa; Merck Millipore, Billerica, MA, USA). The detection media contained the prepared CF660R and Cy3-labelled detection antibodies for each antigen with final concentrations as follows ...
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bioRxiv - Physiology 2022Quote: ... Metabolites were chromatographically separated in a SeQuant ZIC-cHILIC column (PEEK 100 x 2.1 mm, 3 μm particle size; Merck) at 30°C using a method consisting of a gradient running at 0.25 mL/min from 100% mobile phase B (9:1 acetonitrile:water containing 5 mM ammonium acetate pH 6.8 ...
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bioRxiv - Bioengineering 2022Quote: ... 1μl of a purified chain of payload units were added to aldosterone at the desired concentration along with 1.8μl of 100% FBS (Merck – F7524) and topped up to 9μl in 1xTAE-Mg with 0.5μM of off-target blocking oligos (final concentration of 20% FBS) ...
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bioRxiv - Biochemistry 2020Quote: ... was mixed with unlabeled SARS-CoV-2 spike S1 at increasing concentrations (100 pM–3.2 µM) in the presence of 90% heat-inactivated human serum (H5667, Merck) and incubated at 4 °C overnight ...
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bioRxiv - Neuroscience 2021Quote: Mice were first anesthetized with isoflurane and then killed with an overdose of sodium pentobarbital (100 mg/kg) and perfused with 0,01M ice-cold PBS (Merck) followed by 4% PFA (Merck or Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were incubated in primary antibodies solution (antibody diluted in PBS containing 3% goat serum, 0.01% Triton x-100 (Merck)) at 4°C over night ...
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bioRxiv - Microbiology 2020Quote: ... The LC flow rate was 300 nL/min and the 100-min linear gradient ranged from 95% solvent A (H2O with 0.1% formic acid (Merck)) to 40% solvent B (100% acetonitrile with 0.1% formic acid) ...
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bioRxiv - Microbiology 2021Quote: ... Waters I-Class Acquity UPLC (Waters, UK) with SeQuant ZIC-HILIC column (100 mm × 2.1 mm) packed with 3.5 μm polyetheretherketone (Merck, Germany) was used for separating c-di-GMP ...
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bioRxiv - Microbiology 2019Quote: ... and two zwitterionic functional groups – sulfoalkylbetaine bonded to (IV.) polymer support (ZIC-pHILIC, 100 × 4.6 mm, 5 µm; Merck) or (V. ...
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bioRxiv - Physiology 2020Quote: ... The tissue block was dehydrated by using an increasing ethanol series (70%, 80%, 90%, 100%; Merck KGaA, Darmstadt, Germany) and embedded in methyl methacrylate (MMA ...
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bioRxiv - Cancer Biology 2020Quote: ... and equipment were cooled on ice and coated (0.1% Triton X-100 [Sigma-Aldrich 93443] in filtered PBS (Merck Millipore SLGS033SS and Gibco 14190-094) ...
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bioRxiv - Biophysics 2020Quote: ... beads were removed and recombinant dynein was concentrated in a 100 KDa cut-off filter (Amicon Ultracel, Merck-Millipore) to a final volume of 500 μL ...
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bioRxiv - Microbiology 2020Quote: ... 96-well plates with confluent cell layers were fixed and virus was inactivated by immersion in 100% methanol (MERCK) for 20 min ...
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bioRxiv - Immunology 2021Quote: Viral vectors were produced by triple transient transfection of HEK-293Ts in 100 mm plates using GeneJuice (Merck – 70967) with a total of 12.5 μg of DNA ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant and myelin layers were discarded and the pellet resuspended in 100 μL FACS buffer (PBS, 0.1% low endotoxin bovine serum albumin (BSA, Merck), 25 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... cells were permeabilized with 0.1% Triton X-100 for 3 minutes and then blocked with 5 or 10% bovine serum albumin (BSA, Merck) in PBS for 20 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... except that the resuspension of protein precipitate was in modified denaturing urea buffer (8 M urea, 2% SDS, 100 mM HEPES-KOH pH 7.4, 10 mM EDTA and protease inhibitor [Calbiochem, now Merck, https://www.merckmillipore.com] containing 50 mM N-ethylmaleimide (NEM ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells not set aside for surface antigen staining were incubated in PBS containing 0.1% Triton X-100 (X100, Merck) for 5 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... AAV was filter-dialyzed with 1X PBS containing 0.001% Tween-20 using an Amicon Ultra 100 kDa MWCO column (Merck). The titer of the purified AAV was determined ...
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bioRxiv - Neuroscience 2024Quote: ... concentrated using 100-kDa molecular weight cut-off (MWCO) filters Centricon plus-20 and Centricon plus-2 (Merck-Millipore), aliquoted and stored at - 80°C ...
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bioRxiv - Neuroscience 2024Quote: ... AAVs in the cells and supernatant were purified by iodixanol gradient and were concentrated in phosphate-buffered saline (PBS) containing 0.001% Pluronic F68 using Amicon Ultra 100 K filter units (Merck). The titer of AAV was determined with a real-time PCR thermal cycler (Dice ...
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bioRxiv - Microbiology 2024Quote: The chromatographic separation for metabolite profiling was performed using a SeQuant ZIC-pHILIC (2.1×100 mm, 5-μm particle) column (Merck) at 40°C ...
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bioRxiv - Cell Biology 2023Quote: ... the TurboID-CpOGACD or TurboID-CpOGADM expressing HEK293T cells were labeled with 10 to 100 µM biotin (Merck, B4501) in the medium for 15 min to 3 h ...
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bioRxiv - Biophysics 2023Quote: ... in digestion buffer (800 mM guanidine HCl, 2 mM CaCl2, 0.5 % Triton X-100, in 50 mM TRIS, #8382J008706, Merck), overnight at 50°C ...
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bioRxiv - Cancer Biology 2023Quote: Proteins were extracted by RIPA lysis buffer (EcoTech Biotechnology, RIPA-100) containing cOmplete™ protease inhibitor cocktail (Merck, 11697498001), PMSF (Merck ...
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bioRxiv - Bioengineering 2023Quote: ... for 20 min at room temperature (RT) and permeabilised overnight at 4 °C in 0.5% Triton X-100 (Merck) solution in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was concentrated to 10% of the initial volume using a 100 kDa cutoff Amicon filter (Merck Millipore).
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bioRxiv - Neuroscience 2024Quote: Whole iPSC-brain organoids were permeabilized with 0.5% Triton X-100 in blocking solution with 2% Bovine Serum Albumin (BSA; Merck) in PBS for 3 hours at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... plates were washed (6 x 300 µL PBST) and 100 µL of pre-warmed 3,3’,5,5’ tetramethylbenzidine (TMB) solution (Merck-Sigma) was added to each well ...
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bioRxiv - Biophysics 2023Quote: ... The onion cells were prepared by dissecting a 5 mm x 5 mm section of the abaxial epidermis from a brown onion and mounting the membrane (5 mm x 5 mm) onto a #1.5 coverslip with 100 µl of neat Lugol’s iodine solution (62650; Merck, Germany) to produce a stained specimen.
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bioRxiv - Cancer Biology 2023Quote: ... the tissue was minced and incubated overnight on an orbital shaker with 100 U of hyaluronidase (Merck, cat# H3884) and 1,000 U of collagenase type III (Worthington Biochemical ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysis of all samples was performed after two washes with ice-cold PBS by incubating cells for 20 min in ice-cold 1% Triton X100 in PBS with protease inhibitor cocktails (Merck, Calbiochem set III, Cat. No. 539134), subsequent thorough scraping and sonication.
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bioRxiv - Microbiology 2020Quote: ... First strand cDNA preparations were diluted 1:10 with RNase-free water and subjected to RT-qPCR using SYBR Green JumpStart Taq ReadyMix (Merck Life Science UK Ltd., Gillingham, UK), with primers specified in Table S2 ...
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bioRxiv - Neuroscience 2023Quote: ... At 5 hpf embryos were placed into 10 cm Petri dishes containing E3 medium (control) and E3 medium with 1 μM VPA (Sigma-Aldrich, P4543; Merck Life Science Srl, Milan, Italy) for 24 or 48 h ...
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bioRxiv - Evolutionary Biology 2020Quote: ... at 4°C in dialysis buffer (100 mM NaCl, 20mM Tris pH 7.4, containing 15 μl bovine thrombin (605157, Merck Millipore) to cleave the His-tags) ...
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bioRxiv - Biophysics 2019Quote: ... The excess monomeric species was removed by multiple filtration using a 100 kDa cutoff centricon (Amicon, Merck Millipore, MA, USA) to enrich the population of the oligomeric species ...
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bioRxiv - Biochemistry 2019Quote: ... Then, the aqueous phases were immediately mixed with neutral phenol (400 μL) and 1,3-bromochloropropane (BCP, 100 μL) (Merck, 8.01627.0250); or neutral phenol (Roti-Phenol ...
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bioRxiv - Microbiology 2019Quote: ... Solid phase synthesis took place on a CF peptide synthesizer (Intavis) using a Rink Amide LL resin (100 µmol; Merck) and N(α)-Fmoc amino acids ...