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Citations for Merck :
4501 - 4550 of 6398 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... cells were washed twice with PBS and then cultured in a starvation medium composed of DMEM:F12 (1:1) supplemented with BSA (0.3% Sigma-Aldrich/Merck), L-glutamine ...
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bioRxiv - Cancer Biology 2024Quote: ... pLKO.1-puro-shRNACDK12 (TRCN0000196423 and TRCN0000368335) and pLKO.1-puro-shRNABRCA1 (TRCN0000244984 and TRCN0000244987) were purchased from Merck.
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bioRxiv - Biophysics 2024Quote: ... Total DNA was extracted by adding 1 mL of a 25:24:1 phenol:chloroform:isoamyl alcohol mixture (Merck, Darmstadt, Germany) along with 1 g of acid washed glass beads (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: A digestion buffer comprising of 0.05 mg/mL activated DNAse I (DNase I with HBSS in a 1:1 ratio, Merck), 10 mg/mL Collagenase (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... 8.75 μl bead stock (1:1 mix of Sera-Mag SpeedBeads GE45152105050250 and GE65152105050250 at 20 μg/μl, Merck) and 875 μl acetonitrile ...
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bioRxiv - Bioengineering 2024Quote: ... EDC-MG) or with the addition of 1 mL of 10 mg mL−1 bovine serum albumin (Merck A2153) in PBS instead of thrombin (BSA-functionalized microgels ...
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bioRxiv - Cell Biology 2024Quote: ... The GLP-1 concentrations of secretion and lysate samples were measured using GLP-1 ELISA kit (Merck, EGLP-35K). Total protein concentrations of organoid lysates were measured using Pierce™ BCA Protein Assay kit (23227 ...
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bioRxiv - Microbiology 2023Quote: ... Five μl of each sample or standards (ATP, AMP or AMP) were injected into the SeQuant ZIC-pHILIC column (Merck, PEEK 150 × 2.1 mm, 5 μm) and the HPLC system was run with a flow rate of 0.2 ml/min as previously described (Kästle et al ...
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bioRxiv - Plant Biology 2021Quote: ... A dilution series ranging from 1.25 μg ml−1 to 10 μg ml−1 bovine serum albumin (BSA, Merck KGaA) was used for calibration and processed together with the samples.
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bioRxiv - Cell Biology 2020Quote: 1×106 wt and hem1-/- DCs were spun down and lysed in 50µl 1x lysis buffer (1ml 10x RIPA buffer - NEB, 9ml H2O, 1 tablet PhosStop and 1 tablet protease inhibitor mini - both Merck). Lysates were spun at full speed for 10min at 4°C and supernatants transferred to new tubes ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse monoclonal antibodies directed against paxillin (# 5H11, IF dilution, 1:100) and talin (#TA205, IF dilution, 1:100) were from Merck Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... product T7451; used at 1/500) and CHMP1B (rabbit polyclonal antibody, ATLAS product HPA061997; used at 1/500) were from Merck; CHMP2A (rabbit polyclonal ...
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bioRxiv - Developmental Biology 2021Quote: ... for 5 min at room temperature to loosen the zona pellucida and then treated with a hypotonic solution (1:1 mixture of 1.2% sodium citrate and 60% fetal bovine serum, FBS; Merck KGaA) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies, anti-rabbit IgG-HRP (1:5000, #7074, CST) or anti-mouse-HRP (1:10000, #12-349, Merck Millipore) in TBST containing 5% milk for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at room temperature in darkness with the primary antibody solution containing mouse anti-vesicular Glutamate Transporter 1 (vGLUT1, 1:1000, MAB5502, Merck) or rabbit anti-Glutamate Decarboxylase 65-67 (GAD 65-67 ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were maintained under aseptic conditions in a 1:1 mixture of DMEM/F12 (DF-041-B; Merck Millipore) supplemented with 1% antibiotic/antifungal (15240062 ...
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bioRxiv - Bioengineering 2021Quote: ... The LCLs were maintained under aseptic conditions in a 1:1 mixture of Dulbecco’s modified Eagle medium and nutrient mixture F-12 (DMEM/F12; DF-041-B; Merck Millipore) supplemented with 1% antibiotic/antifungal (15240062 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at room temperature in darkness with the primary antibody solution containing mouse anti-vesicular Glutamate Transporter 1 (vGlut1, 1:1000, MAB5502, Merck) and rabbit anti-Glutamate Decarboxylase 65-67 (GAD 65-67 ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies fused to HRP were used for detection (Goat anti-mouse HRP 1:3000, BioRad; Goat anti-rat HRP 1:3000, Merck Millipore ...
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bioRxiv - Genomics 2021Quote: ... The pellet was resuspended in PBS and the cells were then separated into three reactions: (1) added 1:20 volume of 1M NAI (03-310, Merck, 25 µl of NAI in 500 µl of infected cells ...
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bioRxiv - Immunology 2023Quote: 200 μg of HLA.A2-peptide complex in PBS was concentrated to 1 mg ml−1 with the use of Amicon Ultra Centrifugal Filters (Merck), adjusted to pH 8.3 by adding freshly prepared NaHCO3 (0.1 M final concentration ...
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bioRxiv - Molecular Biology 2023Quote: ... To deplete Xrn1 and Dcp2 with the AID tags (Nishimura et al., 2009; Morawska and Ulrich, 2013) 1-Naphthaleneacetic acid (1-NAA; N0640-25G, Merck) was added to a final concentration of 1 mM ...
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bioRxiv - Genetics 2023Quote: ... membranes were incubated with an anti-sera peptide based polyclonal rabbit GLK2-antibody (1:3,000) and a peroxidase-conjugated anti-rabbit antibody (1:10,000) (Merck, Darmstadt, Germany) for 1 h at room temperature in TBST [Tris-HCl 20 mM (pH 7.6) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with a rabbit anti-Kv4.3 primary antibody (1:10000, Alomone Labs) together with chicken (G)/mouse (L) anti-TH primary antibody (1:1000, Abcam / Merck) in a carrier solution (1% NGS ...
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bioRxiv - Cancer Biology 2023Quote: ... one mouse was exposed by drinking water to a mixture of BPA (1 µM, CAS no. 80-09-1, Merck) and DEHP (1 µM ...
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bioRxiv - Immunology 2024Quote: ... and passaged two times on MDCK-II cells with FBS-deficient medium containing 1% penicillin/streptomycin and 1 µg/ml of N-tosyl-L-phenylalanine chloromethyl ketone-treated trypsin (TPCK-trypsin, Merck KGgA ...
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bioRxiv - Cancer Biology 2023Quote: ... depending on manufacturer-suggested minimum seeding density) with lenti/retroviral supernatant in a 1:1 volumetric ratio and 8 µg/mL hexadimethrine bromide (Merck), before centrifugation at 900g for 30 min and returning to incubation at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... washed in TBS-T and incubated with horseradish peroxidase–conjugated anti-rabbit (1:2000, #7074P2, Cell Signalling or 1:80000, #A0545, Merck) and anti-mouse (1:2000 ...
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bioRxiv - Immunology 2022Quote: Heparinised blood was diluted with PBS at a ratio of 1:1 and overlaid onto Histopaque 1083 (Sigma-Aldrich, Merck) density gradient medium using SepMate™-50 centrifugation tubes (Stem Cell Technologies) ...
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bioRxiv - Bioengineering 2022Quote: ... Disruption buffer needed to be extensively washed away before adding 1 ml 1 μM Atto590-NHS ester (NHS-Atto590, #79636, Merck) in PBS to each gel ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then placed in 10% horse serum/PB for 1 h before being incubated in primary goat anti-ChAT (1:500; #AB144P, Merck) for 2 d at 4°C to aid identification of CINs ...
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bioRxiv - Neuroscience 2024Quote: ... re-blocked with PBST and 5% skim milk and incubated with primary antibody against rabbit anti-SOD1 (1:2000, Enzo, NY, USA)) and rabbit anti-GAPDH (1:10,000 Merck, G9545) overnight at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... was incubated with wild-type or mutant PatZ (425 nM) in the presence of 1 mM AcCoA (Cayman) or 1-10 mM of AcP (Merck) in an assay buffer (25 mM sodium phosphate pH 8.0 ...
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bioRxiv - Plant Biology 2024Quote: ... Waters Inc.) was conditioned with 1 mL of 100% methanol and 1 mL of deionized water (Milli-Q, Merck Millipore). After the conditioning steps each sample was loaded on SPE column and flow-through fraction was collected with the elution fraction ...
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bioRxiv - Biochemistry 2024Quote: ... Nuclease reaction products were separated at a flow-rate of 1 ml*min-1 on a Chromolith Performance RP-18e 100 x 46 mm column (Merck) equipped with a guard cartridge ...
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bioRxiv - Pathology 2024Quote: ... after an overnight fasting followed by an oral gavage of glucose (1 g. kg-1 of body weight; G8769, Merck), glycaemia was measured at 0 ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated overnight at 4 °C with blocking solution for 1 h and then with mouse monoclonal anti-NeuN or SMI 32 antibody (1:200; Merck Millipore ...
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bioRxiv - Bioengineering 2024Quote: A 5 mL Terumo syringe containing a solution of 80 mg mL−1 uncrosslinked GelMA and 2.5 mg mL−1 LAP (Merck 900889) in PBS was loaded onto an AL1010 syringe pump (World Precision Instruments) ...
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bioRxiv - Cancer Biology 2021Quote: ... 1-shGAL-9 (MISSION® shRNA library, Merck), psPAX2 and pMD2.G (packaging vectors were a kind gift from Dr ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit anti-SOX2 1:500 (AB5603 Merck Millipore), mouse anti-Tuj1 1:500 (801202 Biolegend) ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-active-ß-catenin (1:300; Merck Millipore), anti-MHC (#M4276 1:200 Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... 1:200 rabbit anti-Ect2 (07-1364; Merck). Secondary antibodies used were 1:500 Alexa flour 488 goat anti-mouse (A11001 ...
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bioRxiv - Cell Biology 2020Quote: For Western blot: α-Puromycin (Merck 1:12,500), α-mouse antibody conjugated with HRP (Bethyl) ...
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bioRxiv - Genetics 2021Quote: ... mouse anti-NR1 (1:1000, Merck Millipore, UK), rabbit anti-PSD95 (1:2000 ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-Actin(20-33) (1:1000, Merck #A5060), anti-mNeonGreen [32F6] ...
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bioRxiv - Neuroscience 2021Quote: ... peroxidase-conjugated (1:4000, Merck EMD Millipore, Ap132P). Immunoreactivity was revealed using the Clarity™ ECL Western Blotting Substrate kit (BioRad ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-SOX2 1:500 (AB5603 Merck Millipore), mouse anti-TUJ1 1:500 (801202 Biolegend) ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-Histone at 1:100 (Merck #MAB3422), mouse anti-γ-tubulin at 1:250 (Sigma-Aldrich # T5326) ...
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bioRxiv - Developmental Biology 2020Quote: ... or an anti-GAPDH antibody (1:104; Merck) overnight at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... 1% benzonase (25 U/μL; Merck,; Kenilworth, NJ), 1% sodium dodecyl sulfate (SDS ...