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Citations for Merck :
401 - 450 of 2444 citations for Rat Insulin Like Growth Factor Binding Protein 5 IGFBP5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the medium was replaced with complete medium without phenol red and 10 μL of 5 mg/mL MTT solution (In vitro toxicology assay kit, MTT-based, TOX-1KT, Merck, Darmstadt, Germany) were added to each well ...
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bioRxiv - Biochemistry 2021Quote: ... protein standards (Merck Sigma-Aldrich) specified in the Supplementary Material were separated on the same column.
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bioRxiv - Microbiology 2023Quote: ... protein A sepharose beads (Merck) were incubated with the cleared supernatant for an hour to remove non-specific binding ...
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bioRxiv - Pathology 2022Quote: ... The elution fraction containing His6-PhoP was dialyzed in Binding Buffer to remove the high concentration of imidazole and concentrated using an ultrafilter (Merck KGaA, Darmstadt, Germany). SDS-PAGE and Western blot with an anti-His tag antibody was used to confirm the purified protein (ABclonal ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were seeded in culture dishes and the specific pharmacological inhibitors radicicol (inhibitor of ATP-binding site of Hsp90, Sigma-Aldrich, Merck, Darmstadt, Germany), cyclosporine A (inhibitor of Cyp activity ...
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bioRxiv - Cancer Biology 2023Quote: ... 250 ng of pTOPflash (wt-TCF) or pFOPflash (mutated-TCF binding site, negative control) reporter plasmids (TOPflash luciferase reporter assay, Merck Millipore, Darmstadt, Germany) were transfected with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... and then clarified via passing through Millex® -GP Fast Flow & Low Binding Millipore Express® PES Membrane 0.22 µm syringe filter unit (SLGP033RS, Merck Millipore, USA).
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bioRxiv - Synthetic Biology 2022Quote: ... the supernatant was sterile filtered (0.22 μm) and afterwards concentrated by the factor of 20 using 100 kDa molecular weight cut-off filters (Merck KGaA, Darmstadt, Germany). To determine the number of released vesicles ...
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bioRxiv - Bioengineering 2022Quote: For purification the Amicon® Pro-Affinity Concentration Kit Protein A with 100 kDa Amicon® Ultra-0.5 Device (Merck Millipore, UK) as used as described by the manufacturer ...
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bioRxiv - Genetics 2021Quote: ... protease inhibitors (5 μM E64, 10 μM Bestatin, 5 μM Pepstatin, 48 hours (Merck Millipore)) ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were washed with TBS 5× 5 min and developed using BCIP/NTP (Merck). The reaction was stopped using MQH2O ...
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bioRxiv - Cell Biology 2019Quote: ... HUVEC primary cells were cultured in all-in-one ready-to-use Endothelial Cell Growth Medium (Cell application Inc. Merck). All reagents specific supplier and identifier are listed in the Resource Table.
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bioRxiv - Bioengineering 2023Quote: ... The pre-adipocytes were plated in custom-made plates (Supp. Fig. 15b) and cultured in growth medium -low glucose (1g l-1) DMEM (Merck) supplemented with 10% FBS (Merck) ...
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bioRxiv - Cell Biology 2024Quote: ... Forty eight hours of post-transfection the viral supernatants were passed through 0.45 μm syringe and the diluted viruses were used to infect cells in growth media supplement with 8 μg/ml of fresh polybrene (Merck) for 8 hr for three consecutive times ...
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bioRxiv - Bioengineering 2020Quote: ... and 100 μl dispensed in triplicate into wells of 96-well ELISA plates (Nunc Maxisorp; Merck Ltd., Poole, United Kingdom) preblocked with 1w/v BSA in PBS and precoated with monoclonal anti–IFN-γ (U-Cytech ...
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bioRxiv - Microbiology 2023Quote: ... Wells were again washed three times with PBS and bound alkaline phosphatase quantified using a p-Nitrophenol phosphate substrate solution for ELISA (Merck) and A405 readings.
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bioRxiv - Microbiology 2023Quote: ... Cells were then washed with three rounds of PBS before incubation in 200 µl p-Nitrophenol phosphate substrate solution for ELISA (Merck) for 10 min ...
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... protein lysates were cleared by centrifugation and protein concentration was measured using Coomassie reagent (Merck). Denatured ...
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bioRxiv - Neuroscience 2021Quote: RNAs with sequences 5’-AAGGAUGGAUGGAG-3’ (healthy) and 5’-AAGCAUGGAUGGAG-3’ (risk) were synthesised by Merck, resuspended in Ultrapure water ...
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bioRxiv - Microbiology 2020Quote: ... 5 μm and 0.22 μm filter pore size (Fig. 5) (Whatman filter, Merck KGaA, Darmstadt, Germany) using sterilised filtration units (Nalgene ...
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bioRxiv - Biochemistry 2021Quote: ... antipapain and pepstatin A) supplemented with 5 mM MgCl2 and 5 KU Benzonase nuclease (Merck Millipore). After incubation at 4°C for 30 minutes to assure the complete lysis ...
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bioRxiv - Plant Biology 2019Quote: ... fresh stem sections were counterstained for 5 min by 5 µg/ml propidium iodide (PI, Merck) dissolved in tap water or 0.01 % Direct Red 23 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% FBS superior (Merck) and 2*105 units/ml Recombinant Human FGF-basic (PeproTech Inc.) ...
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bioRxiv - Developmental Biology 2022Quote: ... Rabbit Aquaporin 5 (Merck, 1:200), Rabbit ERG (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... and 5 μM PD98059 (Merck Millipore), which target p38 and MEK1 ...
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bioRxiv - Cell Biology 2022Quote: ... in 5% acetic acid (1.00063.1000; Merck). Membranes were blocked for 1 h at RT with 5% non-fat milk in PBS-T (1X PBS ...
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bioRxiv - Neuroscience 2022Quote: ... 4-amino pyridine (5 mM, Merck) and TTX (0.5-1 μM ...
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bioRxiv - Pathology 2022Quote: ... for 5 minutes with pronase (MERCK, cat ...
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bioRxiv - Microbiology 2019Quote: ... and 5% water in Acetone (Merck) overnight at -90°C in an Arctiko DP-80 cryo porter (Arctiko ...
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bioRxiv - Microbiology 2021Quote: ... 250 μl of 5% glutaraldehyde (Merck) in 0.2M cacodylate buffer ...
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bioRxiv - Neuroscience 2019Quote: ... Cyanine 5 (#SIC006-5X1NMOL, Merck AG).
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bioRxiv - Cell Biology 2022Quote: ... and 5% ChemiBLOCKER (Merck Millipore, #2170) in 0.1 M NaP ...
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bioRxiv - Microbiology 2023Quote: ... 5% bovine serum albumin (BSA; Merck) was added to cells for 60 minutes to reduce non-specific binding ...
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bioRxiv - Microbiology 2023Quote: ... 5% formic acid (FA, Merck-Millipore) twice for 20 min ...
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bioRxiv - Physiology 2023Quote: ... 5% donkey serum (Merck, Darmstadt, Germany) and 1% DMSO in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... and 5% normal sheep serum (Merck). Incubation with primary antibodies was done for 12 to 72 hours at 8 °C in PBS-T with 3% BSA and 0.02% sodium azide ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... absolute ethanol (Merck: 64-17-5), Oil-red-O (Sigma-Aldrich ...
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... the growth of pure culture of Halomicrobium was obtained in the upper chamber of Millipore® Stericup® filtration system (Merck) (capacity 250 ml ...
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bioRxiv - Molecular Biology 2020Quote: ... When cultures reached the late-exponential growth phase the medium was vacuum filtered using a Stericup with pore size of 0.22 µm (Merck GmbH, Germany) in order to obtain a filtrate containing SIP- and SIP+ ...
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bioRxiv - Synthetic Biology 2020Quote: ... Growth experiments were performed on YNB medium with 1 or 2 % methanol supplemented with 1 g/L yeast extract (Merck 103753). Optical density readings at 600 nm (OD600 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The routinely used growth medium was composed of yeast extract (1%(w/v)) (Fisher BioReagents™) and casein peptone (2%(w/v)) (Merck), and was supplemented with 2% (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... For application in ELISA elution fractions were pooled via Amicon Ultra-15 50k centrifugal filter units (Merck Millipore, Burlington, MA, USA). Elution buffer was chosen for the intended application (coating buffer for sandwich ELISA ...
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bioRxiv - Molecular Biology 2021Quote: ... and isolated murine hepatocytes (muHEP) were seeded on rat-tail collagen (10 µg cm−1, Merck Millipore) coated well plates at a density of 0.1×106 cells per cm² ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Biochemistry 2022Quote: ... Protein standards were obtained from Merck.
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... 2.0 Protein Detection System (Merck Millipore), incubated with Clarity Western ECL (BioRad) ...
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bioRxiv - Biochemistry 2023Quote: In situ protein-protein interaction was evidenced using Duolink® Proximity Ligation Assay (Merck, DUO920082, DUO92004, DUO92008). Cells were fixed with formaldehyde and permeabilized using ice-cold 90% methanol ...
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bioRxiv - Microbiology 2021Quote: ... Clonal populations were obtained by limiting dilution and cell culture growth was monitored with an automatic Muse cell analyzer (Merck Millipore, Paris). Knockout and rescue cell lines were validated by whole-genome sequencing (BGI ...
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bioRxiv - Microbiology 2023Quote: ... Clonal populations were obtained by limiting dilution and cell culture growth was monitored with an automatic Muse cell analyzer (Merck Millipore, Paris).