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Citations for Merck :
401 - 450 of 905 citations for 7 Oxa 12 thia spiro 5.6 dodecan 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Virus containing media was collected and PUER cells were transduced by spin-infection in the presence of 12 μg/ml polybrene (Merck) at 850 g for 2h ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells were differentiated to macrophages by complementing the media with 10 ng/ml phorbol 12-myristate 13-acetate (#P8139, Merck) for seven days ...
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bioRxiv - Immunology 2023Quote: CORA receptors were transduced into a previously described Jurkat-based reporter cell line (12) by addition of lentiviral particles in a MOI of 1 and 5 μg/mL Polybrene (Merck) using spinoculation ...
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bioRxiv - Cell Biology 2024Quote: ... The explants were allowed to attach to the feeder layer before epithelial cell medium (ECM) containing Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham (DNEM-F12, #D8062, Merck) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2024Quote: ... The next day differentiation was induced in DMEM/F12 (Dulbecco’s Modified Eagle’s Medium/ Nutrient Mixture F-12 Ham; Merck Life Sciences), 1X Glutamax (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... and resuspended in 60 μl PFGE wash buffer containing 1 μl lyticase (17 U/μl 10 mM KPO4 pH 7, 50% glycerol Merck L2524 >2000 U/mg) then incubated for 10 min at 50°C ...
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bioRxiv - Physiology 2020Quote: ... Cows treated with the 7-day CO-Synch + CIDR protocol (n = 769) received administration of 100 μg gonadorelin acetate (GnRH; Fertagyl, Merck Animal Health, Madison, NJ) and insertion of a 1.38 g intravaginal progesterone releasing insert (CIDR® Zoetis ...
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bioRxiv - Microbiology 2020Quote: ... 48 and 72 hours) of exposure to simulated solar radiation was performed by using 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) (Sigma Aldrich-Merck KGaA, Darmstadtm Germany) solubilized in ethanol ...
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bioRxiv - Cell Biology 2023Quote: ... to validate the gene expression the plasmid (vide-supra) was transfected into HEK293 cells using PEI (Sigma-Aldrich/Merck Cat. No. 49553-93-7) following an optimized version of the standard protocol(Rajendra ...
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bioRxiv - Microbiology 2024Quote: ... 15 µg of total protein in a volume of 47 µl 1x sample buffer was treated with 7 U of benzonase (#70746; Merck Millipore, Burlington, MA, USA) in dilution buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed and probed with secondary antibody for one hour at RT (anti-mouse IgG, horseradish peroxidase linked; 1:5,000; Merck, Burlington, MA, USA). Bands were visualized using high sensitivity Pierce ECL Plus (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The residue was then resuspended in 100 µl methanol and one µl of each sample analysed by thin layer chromatography (TLC) using silica gel 60 F254 (Merck Millipore, Germany) with chloroform-acetic acid-ethanol (90:5:10 [vol/vol] ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% glycerol) supplemented with 1 mg/ml lysozyme and one tablet of SIGMAFAST Protease Inhibitor Cocktail Tablets EDTA-Free (Merck KGaA, Germany), and disrupted by sonication (10 cycles of 30 sec at 40 % intensity in ice ...
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bioRxiv - Biochemistry 2020Quote: ... RNAs were concentrated by ultrafiltration using Amicon Ultra 3 MWCO centrifugal filters (Merck) and stored at −20 °C ...
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bioRxiv - Biophysics 2021Quote: ... The fractions containing ubiquitin were concentrated by Amicon Ultra-15 3 kDa (Merck) and then loaded onto a HiLoad 16/60 Superdex 75 column (GE Healthcare Life Science ...
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bioRxiv - Plant Biology 2020Quote: ... with 3 kDa molecular weight cut off (Amicon® Ultra, Merck Millipore Ltd.) to remove high molecular weight conjugates of IAA ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was estimated using BCA protein assay kit (Merck, 71285-3, Germany).
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bioRxiv - Immunology 2022Quote: ... MCP-3 and RANTES were quantified using a multiplex kit (MILLIPLEX MAP; Merck, Burlington ...
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bioRxiv - Immunology 2021Quote: ... Peritoneal macrophages were isolated from mice 3 days after injection of thioglycollate (MERCK) and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2022Quote: ... RNAs were concentrated by ultrafiltration using Amicon Ultra 3 MWCO centrifugal filters (Merck) and stored at −80 °C ...
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bioRxiv - Developmental Biology 2019Quote: ... washed again 3 times 15 min and developed using Millipore Crescendo ECL (Merck). For western blot quantification ...
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bioRxiv - Pathology 2020Quote: ... Non-specific binding sites were blocked via incubation in 3% H2O2 (Merck, Germany) and BSA respectively ...
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bioRxiv - Developmental Biology 2019Quote: ... The cells were blocked in 3% BSA (Applichem) and 5% donkey serum (Merck) in PBS for 2 hours at RT ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were mounted using a 3:1 solution of Canada balsam (Merck, # 1016910100) and Histoclear (HS-202 HISTO-CLEAR II ...
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bioRxiv - Cell Biology 2020Quote: ... or rabbit anti-p34-Arc/ARPC2 (Arp2/3, 1:100, Merck, 07-227). This was followed by incubation with appropriate Alexa Fluor-conjugated secondary antibodies (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... reverse: 5’-CCAGGGTGGAGCGGTC-3’) and the KOD Hot Start Mastermix (Merck, Darmstadt, Germany). The plasmids were confirmed by sequencing (Seqlab ...
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bioRxiv - Developmental Biology 2021Quote: ... washed 3 times in PBS with 1x cOmplete Protease Inhibitor Cocktail (Merck, 11697498001).
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore) at 3500 rpm and 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM DTT) and concentrated using a 3 kD MWCO centricon (Merck Millipore). For best performance in the iSAT reaction ...
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bioRxiv - Systems Biology 2022Quote: ... 10+3) were seeded onto fibronectin coated plates (20 µg/mL, FC010 Merck) at a density of 0.25 x 106 cells/cm2 and cultured for three (d3 ...
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bioRxiv - Neuroscience 2023Quote: ... The eluate was concentrated with Amicon Ultra-15 (Merck Millipore, MWCO 3 K) to a protein concentration of 60 µM.
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bioRxiv - Neuroscience 2023Quote: ... anti-cathepsin B (Abcam, ab92955, 1:1,000 and Merck, Ab-3 1:100) and anti-β-actin mouse monoclonal antibody (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... and the pH was adjusted to 5.5 with KOH (1310-58-3, Merck). The plates containing the seeds were stratified for 2 days at 4°C in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... containing 0.17mg/ml tricaine (ethyl-3-aminobenzoatemethanesulfonate, ref. n°E10521, Merck/Sigma-Aldrich). WM983B cells of different lysosome clustering status (spread ...
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bioRxiv - Biochemistry 2023Quote: ... followed by an Amicon ®Ultra centrifugation filter (3 kDa cut-off, Merck). The sample was transferred to a 5 mm NMR-tube (Labscape™ Essence ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples was concentrated by 3 kDa Amicon Ultra centrifugal filters (Merck Millipore, R0NB30416) with 100 mM NH4HCO3 to exchange the buffer and retain globular particles above 3 kDa ...
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bioRxiv - Immunology 2023Quote: ... Tissue slides were blocked in 1X PBS with a 3% BSA (Merck, A7906), 10% Donkey serum (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) was acquired from Merck (Darmstadt, Germany). LNP were produced using microfluidic mixing with the Dolomite Microfluidics system (Dolomite Microfluidics ...
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bioRxiv - Biochemistry 2023Quote: ... PAPS (adenosine 3′-phosphate 5′-phosphosulfate, lithium salt hydrate) was purchased from Merck and stored at -80 °C to afford maximal stability.
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bioRxiv - Plant Biology 2023Quote: ... Solution was subsequently filtered through 3 layers of miracloth paper (Merck 475855-1R) in a 50mL centrifuge tube ...
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Highly amine-reactive graphene-oxide EM grids for biochemical surface modification in aqueous bufferbioRxiv - Molecular Biology 2023Quote: ... 4-Amino-1-butanol (#178330) and 3- glycidoxypropyltrimethoxysilane (#440167) were obtained from Merck. Gold nanoparticle- PEG-amine conjugate was custom-synthesized by NanoPartz (Loveland CO ...
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bioRxiv - Immunology 2023Quote: ... The slides were blocked overnight in PBS solution containing 3% BSA (Merck, #A7906), 10% donkey serum (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... The clean coverslips were sequentially incubated in 0.5% (3-aminopropyl)triethoxysilane (APTS) (Merck) for 3min ...
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bioRxiv - Evolutionary Biology 2024Quote: ... namely using 1 g/L MS-222 (Ethyl 3-aminobenzoate methanesulfonate, Merck #E10521) and subsequent exsanguination by cutting the gill arches ...
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bioRxiv - Microbiology 2020Quote: ... Fifty µg of protein from each sample were resolved in a 12% (w/v) SDS–PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore). The phosphorylated fractions of the MAP kinase ...
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bioRxiv - Cancer Biology 2021Quote: ... After further washing samples were filtered through a 35μm cell strainer directly into 5ml (12×75) polystyrene round-bottom FACS tubes and counted using a Muse Cell Analyser (Merck Millipore). Single cell suspensions were blocked with 10% mouse serum and 1% Fc block prior to incubation with cell surface fluorophore conjugated primary antibodies (Supp table 2) ...
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bioRxiv - Biochemistry 2022Quote: ... were separated on 0.1% SDS polyacrylamide gels (6–12% w/v depending on the molecular mass of protein) and electroblotted onto Immobilon-PVDF Transfer Membrane (Merck Millipore). Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin ...
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bioRxiv - Microbiology 2020Quote: ... Additionally, 12 negative controls were included for the amniotic fluid sampling, aspirating ultra-pure, nuclease-free water (W4502, Sigma-Aldrich (Merck), Overijse ...
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bioRxiv - Immunology 2022Quote: ... The remaining volume was spun down at 500x g for 5 minutes and resuspended in 12 ml FACS buffer (PBS [Gibco]+ 0.5% BSA [Merck] + 2mM EDTA). The suspension was transferred over a 70 μm SmartStrainer (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 μg of protein per sample was loaded and separated on 8-12% SDS–PAGE gels and electro blotted onto polyvinylidenedifluoride (PVDF) membranes (Immobilon-P PVDF membrane 0.45μm; Merck-Millipore, Burlington, USA). Subsequently ...