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Citations for Merck :
451 - 500 of 905 citations for 7 Oxa 12 thia spiro 5.6 dodecan 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Approximately 100 µg protein of each sample was separated by 12% SDS-PAGE and transferred to an Immobilon-PVDF membrane (Merck Millipore). The blot was blocked with 5% non-fat milk in 1× TBST (20 mM Tris base ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... were electrophoresed on SDS polyacrylamide gels ( NuPAGE™ 4-12% Bis-Tris Protein Gels; InvitrogenTM) and transferred to PVDF membranes (Millipore; Merck). Membranes were blocked in 2.5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... were applied for one hour at room temperature and cells were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; Merck G8294; 1:3000) before mounting with Fluoromount™.
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bioRxiv - Microbiology 2020Quote: ... One hundred mL of sewage samples were filtered with a 0.22-μm-pore-size mixed cellulose ester membrane (Merck Millipore, Billerica, MA, USA). DNA was extracted from the filtered membranes and from 150–200mg of the nonhuman fecal samples using the ZR Fecal DNA MiniPrep™ Kit (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... and the chloroform phase was separated by one-dimensional TLC on high performance TLC aluminum sheets (silica gel 60; Merck Poole, United Kingdom) and developed with chloroform/methanol/ammonium hydroxide (40:10:1 ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.01% bromophenol blue) and loaded onto 10-12% SDS-polyacrylamide gels and proteins were transferred to nitrocellulose membranes (Merck-Millipore, Berlin, Germany). Afterwards ...
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bioRxiv - Molecular Biology 2023Quote: ... and loaded in 4–12% NuPAGE Bis-Tris gel (Fisher, 10247002) for subsequent electrophoresis and transference to nitrocellulose membrane (Merck-Sigma, GE10600003). Precipitated RNA was analyzed by autoradiography and FLAG-PTBP protein levels were measured by western blot.
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bioRxiv - Plant Biology 2023Quote: ... Total proteins were separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene fluoride membranes (Merck Millipore, USA). OsJMT1-HA was detected by immunoblotting with HRP-conjugated anti-HA monoclonal antibodies (1:2,000 ...
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bioRxiv - Cell Biology 2023Quote: ... a cell viability assay was performed after the luciferase assay by adding 12 μL of resazurin (Merck Life Sciences, Cat #: TOX8-1KT) and the cells incubated for an additional 2 hours followed by fluorescence reading on a Promega Glomax multimode plate reader.
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bioRxiv - Genomics 2023Quote: ... a ∼20%-confluent wild-type iPSC culture in a 12-well plate containing 1 ml/well of Essential 8 supplemented with 10 µM ROCK inhibitor (Merck, cat# Y0503) was co-transfected with the pZT-C13-L1 and pZT-C13-R1 plasmids (Cerbini et al ...
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bioRxiv - Immunology 2023Quote: ... Equal amounts of protein were loaded and separated by electrophoresis on 12% SDS-PAGE gels and subsequently transferred to polyvinylidene fluoride (PVDF; Merck-Millipore, Germany). After blocking with 10% skim milk for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... each sample was incubated for 12 h (or overnight) in bleaching solution containing hydrogen peroxide solution (H2O2; Cat. No. H1009; Sigma-Aldrich, Merck, Germany) at a final concentration of 15% (v/v)) ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 and incubated overnight at 4°C followed by an secondary anti-rabbit HRP conjugated antibody (12-348, Merck Millipore, Germany) diluted 1:5000 in PBST and incubated for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies used were CYFIP1/2 (Sra-1/PIR121 [14], Rac1/3 (23A8, Merck), Nap1 [14] ...
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bioRxiv - Biochemistry 2019Quote: ... and csm5 were cloned into pACYCDuet-1 (5’-NcoI, 3’-XhoI; Novagen, Merck Millipore), csm4 was cloned into pEHisTEV (5’-NcoI ...
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Obesity alters mobility and adult neurogenesis, but not hippocampal dependent learning in ob/ob micebioRxiv - Neuroscience 2019Quote: ... the solution containing the primary rabbit anti-active Caspase 3 antibody (Merck Millipore, Germany) was applied 1:100 in blocking serum (3 % NGS + 0.1 % Triton X-100 in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The Mpro fractions were concentrated using 3 kDa Amicon Ultra Centrifugal Filters (Merck Millipore) and the Mpro protein was further purified by size exclusion chromatography using a HiLoad Superdex 200 column (Cytiva ...
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bioRxiv - Neuroscience 2020Quote: ... the plate was blocked with blocking buffer (3% BSA (Merck Millipore, MA, USA 820451) in PBS ...
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bioRxiv - Microbiology 2020Quote: ... 13 mM glycine and 3 μM thiamine (all compounds from Merck Millipore, Darmstadt, Germany).
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bioRxiv - Biophysics 2022Quote: ... cells were incubated with BioTracker 609 Red Ca2+ AM dye (3 μM, Merck Millipore) in fresh medium for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... with a Chromolith® RP-18 endcapped 5-3 guard cartridges (Merck, Darmstadt, Germany), operated under a flow rate of 0.3 mL/min ...
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bioRxiv - Biophysics 2022Quote: ... Free dye was removed using a 3 kDa cutoff Amicon centrifugal concentrator (Merck, NJ), and the buffer was exchanged to 50 mM sodium phosphate ...
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bioRxiv - Biochemistry 2021Quote: ... and the protein was concentrated using Amicon 3 kDa MWCO centrifugal filters (Merck Millipore). The protein concentration was calculated using the measured absorbance at 280 nm under native conditions and assuming an extinction coefficient of 19940 M-1 cm-1.
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bioRxiv - Bioengineering 2019Quote: ... Eluted proteins were dialyzed using a 3 kDa membrane D-Tube Dialyzer (Merck, Germany) to remove the imidazole in Storage Buffer (200 mM ammonium acetate ...
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bioRxiv - Plant Biology 2020Quote: ... strand exchange is initiated by adding 3 mM ATP and 3.1 μM SSB (Merck). Aliquots are stopped at indicated times by addition of 12 μM EDTA and 0.8 % SDS ...
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bioRxiv - Molecular Biology 2021Quote: ... concentrated using an Amicon Ultra-0.5 3 kDa centrifugal filter device (Merck Millipore, #UFC500324) at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: ... further immunolabeling was used either against Vesicular Glutamate Transporter 3 (1:2000, VGluT3; Merck, Cat#AB-5421 ...
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bioRxiv - Biochemistry 2021Quote: ... Wells were washed 3 times with 200 µL PBS containing 0.1% Tween-20 (Merck) (PBS-T ...
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bioRxiv - Bioengineering 2022Quote: ... The sample was centrifuged using Amicon Ultra filters (Merck, 3 kDa molecular mass cutoff) at 13,000 × g for 15 min to remove polymers ...
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bioRxiv - Biochemistry 2022Quote: ... excess cisplatin was removed by centrifugal filtration (Merck, Amicon® Ultra 3 kDa filters) at 14,000 rcf for 10 minutes at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... and 5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP, 11383221001, Merck-SIGMA). Sections were mounted using DPX (6522 ...
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bioRxiv - Molecular Biology 2022Quote: ... faecalis was diluted 1:100 into 3 L of Brain heart infusion (BHI, Merck) and grown at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by 3 PBS washes and blocking with 5% bovine serum albumin (BSA; Merck) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... ΦKZ014 was concentrated by centrifugal filtration (3 kDa cut-off, Amicon Ultra, Merck-Millipore). Protein concentration of the purified ΦKZ014 was estimated by correlation of band thickness in Coomassie staining with BSA at ∼50 kDa in SDS-PAGE ...
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bioRxiv - Neuroscience 2023Quote: ... the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay was used to analyze the cell viability of SH-SY5Y cells ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was concentrated with an Amicon Ultra-4 (Merck Millipore, MWCO 3 K) to a concentration of 726 µM ...
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bioRxiv - Biophysics 2023Quote: ... they were incubated with NaOH 0.1M and (N-[3(Trimethoxysilyl)propyl]ethylenediamine) (Sigma Merck) for 3 minutes each step ...
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bioRxiv - Bioengineering 2023Quote: ... 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Merck (Germany). The U-87 cell line was a kind gift from Esendagli Group at Hacettepe University (Turkey) ...
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bioRxiv - Microbiology 2023Quote: ... 2–3 mL of stock solution was applied onto preparative TLC plates (glass, Merck, 20×20 cm ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The gelatinous egg sack was removed using 3 % L-cysteine (Merck Millipore, Burlington, MA) and followed by microinjection of shRNAs ...
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bioRxiv - Biophysics 2024Quote: ... 1 mM DTT) and concentrated using a 3 kDa MWCO Centriprep concentrators (Merck Millipore). For best performance in the iSAT reaction ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS) polyacrylamide gels and blotted onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Darmstadt, Germany). After blocking for 1 h with 5% bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... Suspensions were passed through a 40-µm strainer into a 50 ml tube and rinsed again in 12 ml of DMEM with 10% Fetal bovine serum (FBS) (Merck, Kenilworth New Jersey) in 15 ml tubes ...
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bioRxiv - Immunology 2022Quote: ... A 15 ul of lysate was resolved on 12% SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore, Burlington, MA). The membrane was blocked in 5% powder milk overnight in 4°C ...
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bioRxiv - Zoology 2020Quote: ... Wing scales were removed using the methods published Carvajal et al.12 and were then mounted on a glass slide and cover slip with Aquatex® (Merck, Darmstadt, Germany). Wing images were captured with 3.5x magnification using Nikon SMZ800 dissecting microscope ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 1,500 worms per replicate were harvested at 12 h post L4 and washed across a Steriflip® 20 μm nylon mesh filter (Merck; Darmstadt, Germany) with M9 buffer ...
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bioRxiv - Plant Biology 2019Quote: ... Supernatant was then passed through a 3 kDa cutoff centrifugal filter (cellulose Amicon®, Merck) (1 h at 14000 g at 4 ºC ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 mM MgCl2) using Amicon Ultra-0.5 mL Centrifugal Filters (100 K MWCO, Merck Millipore). For each MST assay ...
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bioRxiv - Immunology 2021Quote: ... and washed by ultracentrifugation at 4°C using a 3 kDa filter (UFC900324, Merck-Millipore) to remove imidazole ...
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bioRxiv - Synthetic Biology 2020Quote: ... The samples were subjected to ultrafiltration using 3-kDa filters (Amicon Ultra 3K device, Merck). After lyophilization ...