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Citations for Merck :
4401 - 4450 of 4915 citations for Human Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... cell culture supernatant of HUVECs was concentrated to 1 mL using Amicon-Ultra 15 Centrifugal Filter Units (UFC901096, Merck) and incubated with 0.5 µg of Alexa 488 labeled anti-CD9 ...
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bioRxiv - Molecular Biology 2020Quote: ... EmbryoMax KSOM medium with 1/2 amino acids without BSA (Reagent setup; Merck Millipore, cat. no. MR-107-D), BSA powder (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4 °C with 100 µL of lysate diluted at 1:40 in carbonate buffer (Merck). After a blocking step with PBS with 4 % milk for 1 h ...
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bioRxiv - Physiology 2020Quote: ... rabbit polyclonal (RP) against phosphorylated Tyr 1077 ObRb (pTyr1077ObR; 1:500, cat# 07-1317, Merck Millipore, Burlington, Vermont, USA), GP against phosphorylated Tyr 1138 ObRb (pTyr1138ObRb ...
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bioRxiv - Microbiology 2019Quote: ... 1 μL of the reaction was spotted onto aluminium backed silica gel plates (5735 silica gel 60 F254, Merck) and dried before being subjected to Thin Layer Chromatography (TLC ...
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bioRxiv - Biophysics 2019Quote: ... we incubated clean glass cover slips (glued to 8-well LabTek chambers) with 30 µg ml−1 fibronectin (Merck) in 1 x PBS for 2 h at 37 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We next centrifuged the mix (1 min, 13,000 g) through a Durapore PVDF 0.22-μm filter (Merck Millipore, US). We injected the CRISPR-Cas9 solution into the male pronucleus of fertilized zygotes with a motor-driven manipulator-based microinjection stage ...
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bioRxiv - Cell Biology 2021Quote: ... the nuclear pellet was resuspended in 0.5 mL of ice-cold RIPA buffer supplied with 1 U/mL Benzonase (Merck), and incubated on a rotator for 1 h at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.4) (final concentration: 12mg/mL) containing 5 μL Benzonase (final concentration: 1 μL benzonase/mL (MERCK, 71205-3). After dissolving ...
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bioRxiv - Biophysics 2021Quote: ... Myosin II filaments were further diluted in KMEH to 200 nM and added to the actin filaments bound to the SLB by His6-Curly by replacing 1/10 of the sample buffer with the myosin II filament solution and supplemented with 0.1 mM Mg-ATP as well as a mix of 1 mM Trolox (cat. no. 648471, Merck), 2 mM protocatechuic acid (cat ...
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bioRxiv - Biochemistry 2021Quote: ... and soluble protein was clarified by centrifugation (50,000×g, 1 h, 4°C) and subsequently passed through a 0.22-μm filter (Merck Millipore).
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bioRxiv - Neuroscience 2021Quote: The following antibodies were used for immunoassays: primary antibodies - rabbit polyclonal anti-RTN3 (#ABN1723, Merck Millipore; 1:400 dilution), monoclonal anti-NF200 (#N5389 ...
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bioRxiv - Microbiology 2021Quote: DF-1 cells were seeded into a Chambered #1.0 Borosilicate Coverglass slide (Nunc, Lab-Tek, Sigma-Aldrich, Merck, UK) at a density of 8 × 104 per well and cultured in 1ml of DMEM supplemented with 10% hiFBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Then the culture medium was removed and replaced with RPMI medium containing 1 µg/ml D-Actinomycin (Merck, Germany) and different samples of rhTNFα or rhTNFα incubated with TNFα antagonist ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C for 60 minutes followed by proteinase K solution treatment (7.5 μg ml−1 proteinase K; Merck, Darmstadt ...
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bioRxiv - Cell Biology 2021Quote: ... brain sections were subjected to immunohistochemistry using a mouse anti-NeuN antibody (MAB377; Merck, Kenilworth, NJ, USA; 1:500) followed by incubation with an anti-mouse IgG antibody (Vector Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with sgRNA lentiviruses at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours and then reprogramming was initiated by addition of reprogramming medium ...
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bioRxiv - Bioengineering 2022Quote: ... samples were fixed in a solution of 4 % (v/v) paraformaldehyde supplemented with 1 % (v/v) glutaraldehyde (Merck, USA) overnight at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then trypsinized and placed in cell culture inserts (1 × 106 cells per insert) with PTFE membrane (Merck) in 6 well plates for overnight cultivation ...
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bioRxiv - Microbiology 2022Quote: ... two ml of bacterial suspension (IPO 2222 WT or selected □D5-resistant IPO 2222 Tn5 mutants) adjusted to 106 CFU mL-1 in sterile Ringer’s buffer (Merck) was sprayed using a manual sprayer on the adaxial surface of each detached leaf ...
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bioRxiv - Microbiology 2022Quote: ... HRP-conjugated anti-goat or anti-mouse IgG (dilution 1:1,000) were added and protein complexes were visualized using o-phenylenediamine (Merck). The absorbance was then measured at 490 nm employing PowerWave HT (Bio-Tek Instruments ...
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bioRxiv - Cell Biology 2022Quote: ... and were stained on day 6 after the last 1-hour stimulus with dimethyl-methylene blue (DMMB; pH 1.8; Merck) metachromatic dyes as previously described 15 ...
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bioRxiv - Microbiology 2021Quote: Polar metabolites were first extracted from the frozen SHIME-samples by means of ultra pure water (0,055 μS cm-1) obtained via a purified water system (VWR International, Merck). For this purpose ...
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bioRxiv - Microbiology 2020Quote: ... anti-A17L (1:500) in combination with horseradish peroxidase (HRP)-coupled secondary antibodies (1:2,000) and Immoblion Forte Western HRP substrate (Merck) or with IR-Dye secondary antibodies (Licor ...
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bioRxiv - Immunology 2021Quote: ... Samples were postfixed with 0.1 M sodium cacodylate buffer supplemented with 1 % osmium tetroxide (Science Services) and potassium ferrocyanide (Merck) and embedded in 1 % agarose (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies diluted in 5% milk/PBS-Tween were: mouse anti-Actin (1:2000; Merck, Germany, Cat. No. MAB1501), rat anti-MYC (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: Type 1 diabetes was induced with a single intraperitoneal (i.p.) injection of 150 mg/kg streptozotocin (STZ; Merck, France) freshly dissolved in 0,9 % NaCl ...
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bioRxiv - Microbiology 2022Quote: ... The sections were then incubated overnight in PBS with 0.2% BSA and 0.05% Tween-20 with primary antibodies directed against SARS-CoV-2 Nucleocapsid Protein (1:500; mouse monoclonal, # ZMS1075, Merck); Iba1 (1 ...
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bioRxiv - Cell Biology 2022Quote: ... The sections were then dried and stained on a heating block with a 1% toluidine blue (Merck, 89640-5G) solution including 2% Borax for 90 s at 60°C ...
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bioRxiv - Neuroscience 2019Quote: ... the slices were incubated in the antibody solution (Anti-NG2 Chondroitin Sulfate Proteoglycan, Rabbit, 1:250, AB5320, Merck Millipore) for 48 h at 4 °C ...
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bioRxiv - Physiology 2019Quote: ... semithin sections (1 µm) were used for light microscopy and stained with a solution of 0.5 % toluidine blue O (Merck); ultrathin sections (75 nm ...
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bioRxiv - Physiology 2020Quote: ... Membranes were probed overnight at 4°C with anti-phosphotyrosine antibody (1/1000, 05-321, clone 4G10, Merck Millipore) in blocking solution ...
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bioRxiv - Cell Biology 2019Quote: ... acetylated α-tubulin (Clone 6-11B-1, Cell signalling Technology) or tyrosinated α-tubulin (Clone YL1/2, Merck Millipore) in blocking solution at 4 °C overnight ...
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bioRxiv - Bioengineering 2020Quote: ... labeled for 30 minutes at 4ºC and washed with PBA (PBS with 0.2% sodium azide [Merck]; 1% BSA [Sigma]).DAPI (final concentration of 1 μg/ml ...
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bioRxiv - Biochemistry 2020Quote: Cells were harvested and lysed using RIPA lysis buffer (Beyotime, China) supplemented with 1% protease inhibitor cocktail (MERCK, Germany). After centrifugation at 14 ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Pathology 2021Quote: ... 96-well plate was coated with 1 μg/ml monoclonal anti-lubricin antibody clone 9G3 (Merck Millipore, Billerica, MA) in TBS at 4°C over-night ...
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bioRxiv - Microbiology 2021Quote: ... Isolated lipids were dissolved in 30 μL of chloroform:methanol (2:1, v/v) and 5 μL loaded on thin-layer chromatography (TLC) silica gel plates F254 (Merck). Lipids were separated in chloroform/methanol/water (20:4:0.5 ...
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bioRxiv - Microbiology 2019Quote: ... Reaction products were then visualized by spotting 1 μl on PEI-cellulose thin layer chromatography (TLC) plates (Merck Millipore) and separated using 1 M KH2PO4 ...
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bioRxiv - Genetics 2021Quote: ... a mouse anti-actin monoclonal antibody (#MAB1501R, used at 1:5,000 dilution for the Western blot analyses, Merck Inc.); a mouse anti-glyceraldehyde-3-phosphate dehydrogenase monoclonal antibody (#MAB374 ...
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bioRxiv - Cell Biology 2021Quote: ... Surfaces were functionalised by flooding the device with 1% (v/v) trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Merck) in HFE-7500™ (3M™) ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were passaged at 70 – 90% confluency using 1% PBS and trypsin/EDTA (0.25%,0.02%) in T75 tissue culture flasks (Merck, Australia) and cultured at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... pellets were resuspended in urea lysis buffer (8 M Urea, 50 mM Tris pH 7.5, and 1:200 freshly added protease inhibitor cocktail (Merck)) ...
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bioRxiv - Biochemistry 2021Quote: ... Appropriate fractions were concentrated to 3-0.7 mg mL−1 using 30,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Cell Biology 2021Quote: ... cells were starved overnight in serum-free DMEM and then treated for 1 hour with 5 ng/ml TGFβ1 (Preprotech) or with 5 μM SB431542 (Merck), and intensities quantified by ImageJ ...
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bioRxiv - Biophysics 2020Quote: ... the purified mEGFP-tagged NbALFA was concentrated to 1 mg/ml using an Amicon Ultra 30K filter (Merck Millipore) and stored at -80 °C before use ...
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bioRxiv - Cell Biology 2020Quote: ... H4S1 phosphorylation was detected using the anti-phospho histone H4/H2A S1p antibody at 1:1000 (Upstate/Merck-Millipore); sf-GFP-Sps1 was detected using JL-8 anti-GFP antibodies (Takara/Clontech ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were washed twice with cytoskeleton buffer and stained with 1:500 30972 Abberior® STAR 635 (Merck) O/N at 4°C ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-mouse IgG HRP antibody (AP192P, Merck Millipore). Protein detection was performed using SuperSignal West Pico PLUS (34579 ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-rabbit IgG HRP antibody (AP182P, Merck Millipore) and 1:5000 dilution of mouse anti-GAPDH (ab8245 ...