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Citations for Merck :
4151 - 4200 of 5744 citations for Trans Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%;1 D 97% 97% Chemical Purity since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... fluorescent visualization of the nonisotopic signals used anti-digoxigenin antibodies conjugated to horseradish peroxidase (anti-digoxigenin-POD; Fab fragment; 1/200; Merck). This was followed by the deposition of biotin tyramide (1:1000 ...
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bioRxiv - Pathology 2023Quote: Cryosections of formalin-fixed lung tissue 7 μm thick were washed in PBS and permeabilized for 1 h with 0.01% Tween 20 (Sigma-Merck, Germany), followed by three washes in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... in Tris- buffered salione containing 0.1% Tween-20 (v/v) (TBST) fore 60 mins at RT and incubated overnight with primary antibody against REST (1:500) (07-579, Merck), H3K9me2 (1:500 ...
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bioRxiv - Molecular Biology 2024Quote: ... the plates were incubated for 1 h at 25°C with peroxidase-conjugated donkey anti-chicken IgY (Merck, NJ, USA) diluted 5000 times with HEPES-EDTA containing 1% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... each sample was pelleted by centrifugation at 3,000 g for 10 min at 4°C and the pellet was resuspended in 100 μl of 1% SDS lysis buffer (1% SDS in 50 mM Tris-HCl (pH=8.0) and supplemented with 1X protease inhibitor cocktail (from 100X; Merck, P8849), 1 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Immunology 2024Quote: ... 130 pg of gRNA and 250 pg of the Cas9 protein were co-injected into a 1-cell stage fertilized embryos of AB WT fish with phenol red (pn. 114537-5G, Merck) as a tracer dye ...
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bioRxiv - Molecular Biology 2024Quote: ... cell pellets were resuspended in 500 μL Nuclear RNA lysis buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2, 1% BSA (Merck, #B9000S), 0.1% Tween (Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... followed by an incubation overnight at 4 °C in blocking solution with anti-puromycin antibody (clone 12D10, MABE343; 1:500) AlexaFluor 488 or AlexaFluor 647 (MABE343-AF488 or MABE343-AF647; Merck). On the following day ...
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bioRxiv - Plant Biology 2024Quote: ... Secondary incubation was completed with a 1:40 dilution of Goat anti-Rabbit IgG 10 nm gold conjugate for a further hour (Merck).
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bioRxiv - Plant Biology 2024Quote: ... AtPFA-DSP1 and AtNUDT17 were resuspended in buffer A (50 mM Tris pH 7.5, 500 mM NaCl, 1 mM DTT, Dnase I and cOmplete™ protease inhibitor cocktail [Merck]), MpPFA-DSP1 and MpNUDT1 were resuspended in buffer B (50 mM K2HPO4/KH2PO4 pH 7.8 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with one or more of the following primary antibodies in blocking buffer overnight at 4°C (rabbit anti-TH, 1:2000; AB152, Merck-Millipore ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples were separated on 12% non-reducing SDS-PAGE gel and cysteine-dimedone protein adducts visualised by western blot analysis using 1:10,000 diluted rabbit anti-cysteine sulfenic acid primary antibody (Merck Millipore) and 1:10,000 diluted horseradish peroxidase conjugated goat anti-rabbit IgG secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: DNA encoding the TBD domain of human ASK1 (residues 88–267) was ligated into pRSFDuet-1 (Merck KGaA, Darmstadt, Germany) using BamHI and NotI sites ...
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bioRxiv - Microbiology 2024Quote: ... the coverslips were fixed in 1% glutaraldehyde in 1x PBS for 30 min and stained with filtered 10% Giemsa (Merck) in 1x PBS for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Then 500 μL Nuclear RNA wash buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2, 1% BSA (Merck, #B9000S), 0.1% Tween (Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... The aspirated follicular fluid was transferred to a 50 ml falcon tube containing 140 µl of 5000 IU/ml Heparin (Merck Millipore). COCs were allowed to sediment and then washed extensively with pre-warmed TCM199 (HEPES-buffered medium 199 ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ml of extract was incubated overnight at 4°C with rotation with 20 µg/ml anti-FLAG M2 antibody (Merck, F3165) and 50 µl Pierce magnetic protein A/G beads (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: To evaluate microscopic features of the captured cells such as morphological types and possible alterations we then used another collection membrane directly stained with hematoxylin and eosin after fixation for 10 min in a fixation solution (100 mL of 70% ethanol, 5 mL of glacial acetic acid and 5 mL of 37% formaldehyde solution – all solutions are from Merck Millipore). Samples were hydrated with distilled water for 5 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... concentrated to 33 mg/ml using an Amicon Ultra 50 ml 10 kDa molecular weight cut-off centrifugal filter (Merck Millipore) and then aliquoted before flash freezing the protein sample in liquid nitrogen ...
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bioRxiv - Microbiology 2019Quote: ... faecal samples were added to 10 ml of Buffered Peptone Water (Fluka Analytical, 08105-500G-F) supplemented with 10 μg/ml Novobiocin (Merck, N1628), and incubated overnight at 37°C with shaking at 220 rpm ...
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bioRxiv - Immunology 2021Quote: ... tumors were enzymatically digested with 2,000 U/mL of DNase I and 2 mg/mL of collagenase IV (both from Sigma Aldrich, Merck, Israel) in HBSS for 30 min 37 □ ...
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bioRxiv - Synthetic Biology 2022Quote: ... This solution was incubated at 37°C for 3-4 h before being transferred to a 250 mL flask containing 20 mL of 425-600 μm glass beads (Merck G9268) and 30 mL of sterile MilliQ water ...
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bioRxiv - Microbiology 2024Quote: ... Transconjugant colonies carrying pCPE16_3blaNDM-1 were selected on LB agar supplemented with 300 µg/mL hygromycin B (PhytoTech Labs, USA) and 2 µg/mL doripenem (Merck, Germany). Conjugation frequencies were calculated using the following formula: Data shown are the mean ± standard deviation of three independent experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... The nuclei pellet was washed once in 5 mL of Honda buffer then purified on a Percoll density gradient as follows: 2 mL of 75% Percoll (Merck, P7828) in Honda buffer topped with 2 mL of 40% Percoll in Honda buffer topped with the nuclei pellet resuspended in Honda buffer in a 15 mL tube ...
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bioRxiv - Cell Biology 2022Quote: ... Four microliters of complex were added to 16 μl of TIRF-Casein buffer supplemented with an oxygen scavenging system (0.2 mg/ml catalase, Merck; 1.5 mg/ml glucose oxidase, Merck; 0.45% (w/v) glucose ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μL of this complex was added to 19 μL of TIRF-Casein buffer supplemented with an oxygen scavenging system (0.2 mg/mL catalase, Merck; 1.5 mg/mL glucose oxidase, Merck; 0.45% (w/v) glucose) ...
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bioRxiv - Cell Biology 2023Quote: ... produced by the mix of 50 ml of sodium hypochlorite 10% (Roth, ref. 9062.3) and 2 ml of 37% hydrochloric acid (Merck, ref. 1.00317.1000). Seeds were then aerated for 10 min in a sterile bench to remove the left-over chlorine gas ...
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bioRxiv - Plant Biology 2023Quote: ... from 25 plants were transferred into a Teflon vessel and digested with 2 mL of 65% HNO3 and 0.5 mL H2O2 (Suprapur; Merck, Darmstadt, Germany) in a MarsXpress microwave digestion system (CEM ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were blocked in 10% normal goat serum in TBS-T for one hour at room temperature and then incubated in the following primary antibodies suspended in blocking buffer overnight at 4°C: pS129 (El-Agnaf laboratory, 1µg/ml 27) and 5G4 (MABN389, Merck, 2µg/ml) combined with either MGO-aSyn (1µg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... Eluted supercomplex was concentrated with a 100 kDa molecular weight cut-off concentrator (Merck Millipore) and further purified by size exclusion chromatography with an Äkta Pure M25 (GE Healthcare ...
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bioRxiv - Physiology 2022Quote: ... 34.5 mg TSP (Trimethylsilyl propanoic acid; used as NMR standard) and 100 mg NaN3 (Merck) were added 59 ...
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bioRxiv - Neuroscience 2021Quote: ... and this solution was ultrafiltered with an Amicon Ultra-15 device (100 kDa, Merck, Germany) to obtain the AAV stock solution for administration to mice.
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bioRxiv - Biochemistry 2019Quote: ... 0.5 % (v/v) Triton X-100 and cOmplete™ EDTA-free Protease Inhibitor Cocktail (Merck). 50 μg of protein extract (estimated by Bradford ...
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bioRxiv - Immunology 2020Quote: ... the Protein G chip was regenerated for 30 seconds with 100 mM Glycin-HCl (Merck) pH 1.5 at 30 µL/min and the process was repeated starting a new cycle with the capture of the potentiating antibody ...
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bioRxiv - Neuroscience 2020Quote: ... The recovered nanoparticles were pooled in Amicon Ultra-4 100 kDa centrifugal filter units (Merck) and concentrated by centrifuging at 2000 ×g until the lipid concentration was increased to 30–40 mM.
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bioRxiv - Biochemistry 2020Quote: ... followed by concentration using Amicon Ultra-15 Centrifugal Filter Unit (100 kDa cutoff, Merck KGaA). The concentrated medium (6 mL ...
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bioRxiv - Microbiology 2020Quote: ... Samples were transferred to polyvinylidene fluoride (PVDF) membranes (Merck Millipore, activated in 100 % (v/v) methanol ...
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bioRxiv - Microbiology 2021Quote: ... Cells were permeabilized with a solution of 0.1% Triton X-100 (Sigma-Aldrich, Merck, UK) for 15 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Samples were concentrated using an Amicon Ultra 100 K spin filter (Merck KGaA, Darmstadt, Germany), if necessary.
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bioRxiv - Biochemistry 2022Quote: ... mPSF:PAP complex was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 100 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing CIV were concentrated with a 100-kDa molecular weight cutoff concentrator (Merck Millipore) and into 50 mM KCl ...
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bioRxiv - Biochemistry 2022Quote: ... Refolded HlyA was concentrated (Amicon Ultra-15 Centrifugal Filter Units, 100 kDa NMWL, Merck Millipore) and remaining urea removed with PD10 desalting column (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... The proteins were concentrated to 50−100 µM using the Amicon Ultra centrifugal filters (Merck). For protein labelling ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein solution was applied to a MonoQ 10/100 column (Sigma-Aldrich, Merck), equilibrated in MonoQ buffer A (40 mM Hepes ...
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bioRxiv - Microbiology 2020Quote: Cells were lysed with phosphate buffer saline (PBS) containing 0.1% Triton X-100 (Merck Millipore), according to a standard procedure (54) ...
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bioRxiv - Microbiology 2020Quote: ... for 90 minutes at 100 V and blotted onto Immobilon-FL PVDF membranes (Merck Millipore). The transfer was performed at a constant voltage of 30 V for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... for 90 minutes at 100 V and blotted onto Immobilon-FL PVDF membranes (Merck Millipore). The transfer was performed at a constant voltage of 30 V for 30 minutes ...
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bioRxiv - Synthetic Biology 2022Quote: ... 100 mM NaCl) using an Amicon® Ultra-15 10 K Centrifuge Filter (Merck Millipore). The protein concentration was measured using the Qubit Protein Assay Kit and Qubit 3 Fluorometer (Thermo Fisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... using a LiChroCART 250 × 4 mm LiChrospher 100 RP 18 column (Merck KGaA, Darmstadt, Germany). The mobile phase was a mixture of water and acetonitrile (70:30 ...
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bioRxiv - Molecular Biology 2020Quote: ... The TDH complex was concentrated in a 100 kDa cut-off Amicon centrifugal concentrator (Merck) at 1,500 rcf to 0.1-0.2 mg/ml ...