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Citations for Merck :
351 - 400 of 593 citations for Recombinant Human Complement Factor H His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: The HCYTOMAG-60K-15 Human Cytokine/Chemokine Multiplex Assay (Merck Millipore) was employed to simultaneously analyze TNF-α ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 IU mL1 human chorionic gonadotropin (hCG; Chorulon; Merck Animal Health) and 1.0 mg mL1 oestradiol ...
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bioRxiv - Immunology 2023Quote: ... and human rIL-2 (30 U/ml) (Roche/Merck, Darmstadt, Germany) to the culture ...
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bioRxiv - Developmental Biology 2023Quote: ... Coating was performed with 20 µg/ml human fibronectin (Merck FC010) in PBS for at least 30’ ...
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Lowering mutant huntingtin by small molecules relieves Huntington’s disease symptoms and progressionbioRxiv - Molecular Biology 2023Quote: ... and anti-eIF4G1 for human samples (1:2000; 07-1800, Merck) or anti-eIF4G3 for mouse samples (1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... and antibodies directed against anti-human mitochondria (1:100, Merck #1273;), CDK2AP1 (rabbit ...
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bioRxiv - Bioengineering 2024Quote: ... anti-human nuclei (HuNu; 1:100; MAB4383, Merck Millipore, MA, USA), anti-Ku80 (1:100 ...
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bioRxiv - Biophysics 2019Quote: ... for 2 h and buffer exchange with centrifugal filter devices (10,000 MWCO, Amicon, Merck Millipore) into storage buffer ...
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Nerve pathology is prevented by linker proteins in mouse models for LAMA2-related muscular dystrophybioRxiv - Neuroscience 2022Quote: ... General histology was assessed after tissue fixation with 4% paraformaldehyde by H&E staining (Merck) or Picro Sirius Red stain (Direct Red 80 (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were incubated for 4 h at 4°C with DAPI (1 µg/mL, Merck) and the following secondary antibodies diluted in blocking buffer ...
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bioRxiv - Immunology 2024Quote: ... cells were treated for 4 h with 5 mg/ml Brefeldin A (Merck, Darmstadt, Germany) after 140 h incubation time ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant MBP fusion protein was concentrated with an Amicon Ultra-15 centrifugal filter unit (Ultracel 3K; Merck Millipore, Darmstadt, Germany) and used directly for electrophoretic mobility shift assay (EMSA).
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed in recombinant tau-lysis buffer (1 mM benzamidine, 1 mM PMSF, 1x cOmplete™, EDTA-free Protease Inhibitor Cocktail mix (Merck), 14 mM b-mercaptoethanol ...
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bioRxiv - Neuroscience 2021Quote: ... the embryos were cultured for 1 h in KSOM medium (Merck, Germany, or ARK Resource, Japan) in a 5% CO2 incubator at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... the embryos were cultured for 1 h in KSOM medium (Merck, Germany, or ARK Resource, Japan) in a 5% CO2 incubator at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... washed with tap water and incubated for 1.5 h in 0.1% (w/v) Sirius red (Merck) in saturated picric acid solution with mild shaking ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... for 30 min and blocked for 1 h with blocking solution (Merck-Millipore, Burlington, MS, USA) at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Protein was digested by incubating membrane at 50°C for 1 h with proteinase K (Merck) diluted in PK-SDS buffer ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by a 3 h blocking step with 5 % NGS (G9023, Merck, Darmstadt Germany) in the respective washing buffer ...
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bioRxiv - Physiology 2021Quote: ... For histological analysis TA sections were stained with hematoxylin and eosin (H&E; Merck, Zug, Switzerland). To determinate fiber type and fiber size sections were blocked and permeabilized in PBS containing 10% goat serum and 0.4% triton X-100 for 30 min before being incubated for 2 h at RT in a primary antibody solution containing BA-D5 ...
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bioRxiv - Neuroscience 2022Quote: ... under gentle agitation for 24 to 36 h in a fixative mixture of 4% acrylamide (Merck), 4% paraformaldehyde and 0.25% VA-044 thermal initiator (Fujifilm ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated 48 – 72 h with sheep anti TPH (1:1000, ab1541, Merck Millipore) primary antibody in carrier solution containing 1% NGS and 1% BSA in PBST at +4°C ...
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bioRxiv - Biochemistry 2020Quote: ... 500 μM ATP and ~0.5 μg commercially available human CyclinB-Cdk1 (Merck). The reaction was carried out at 30 °C and was stopped using SDS-PAGE sample buffer after 5 minutes.
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bioRxiv - Cell Biology 2019Quote: ... Human pancreatic organoids were additionally immuno-stained against Sox9 (primary antibody: Merck Millipore ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... a rabbit anti-human EGFR monoclonal antibody (EMD Millipore-MERCK, Temecula, USA) diluted 1:1000 or a rabbit anti-human VEGF-A polyclonal antibody (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... human tau constructs were expressed in the vector pNG2 (Merck-Novagen, Darmstadt) in E ...
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bioRxiv - Immunology 2021Quote: ... MILLIPLEX MAP Human High Sensitivity T Cell Panel (Merck Cat# HSTCMAG-28SK) were used ...
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: ... 501µg/ml Ascorbic acid and 180⍰µg/ml Human transferrin (Merck, T8158)) and then plated onto non-adherent dishes (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... Immortalized human cerebral microvascular endothelial cell line (hCMEC/D3 Merck Life Science) were cultured on 250µg/ml collagen-I (Advanced Biomatrix ...
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bioRxiv - Developmental Biology 2023Quote: ... and reinjected after 48 hours 7.5IU hCG (human chorionic gonadotrophic hormone; Merck), before overnight mating with F1 or F1 hybrid transgenic mT/mG stud males (original mT/mG transgenic mouse line obtained from Jackson Laboratories ...
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bioRxiv - Biophysics 2023Quote: ... A solution was prepared by mixing 70 µL human plasma fibronectin (Merck), 20 µL green fibrinogen and 910 µL PBS ...
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bioRxiv - Microbiology 2024Quote: ... Human Serum Albumin with or without fatty acids were purchased from Merck. IVIg (Multigam® 5%) ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
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bioRxiv - Immunology 2020Quote: ... urinary human menopausal gonadotropin (hMG) (HMG-lepori 75 UI, Angelini Farmacéutica, S.A, Spain) and recombinant hCG (Ovitrelle 250 micrograms, Merck Serono Europe Limited, Spain) or luprorelin acetate (Procrin 1 mg/0,2 ml ...
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bioRxiv - Developmental Biology 2021Quote: ... the skin sections were stained with hematoxylin and eosin (H&E) and mounted with Entellan® (Merck). The stained slides were imaged using a DM2000 light microscope (Leica biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... Supernatant was harvested 40 h after transfection followed by centrifugation and filtration (0.45 µM; SLHV033RS, Merck Millipore) to remove cellular debris ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed 48 h after transfection in 2x Laemmli buffer containing 200 mM dithiothreitol (DTT, Merck). Lysates were sonicated and separated by SDS-PAGE/immunoblotting using 7,5 % gels ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted proteins were collected after a 72-h incubation by Amicon Ultra-15 Centrifugal Filter Units (Merck) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... 72 and 96 h post electroporation and filtered using filter units (Merck, 0.45 μm membrane pore size). Infectious titers of virus stocks were determined by limiting dilution assay (TCID50/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with PBS and fixed for at least 3 h with 70% ethanol (Merck, 100983). Cells were washed with PBS and analyzed by using the Cell Cycle Kit (Luminex Corporation ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 7.9) on ice for 1 h in the presence of EDTA-free Protease Inhibitor Cocktail (Merck) and cleared by centrifugation at 20,000 g for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 7.9) on ice for 1 h in the presence of EDTA-free Protease Inhibitor Cocktail (Merck). Lysates were cleared by centrifugation at 20,000 g for 15 min ...
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bioRxiv - Plant Biology 2023Quote: ... pericarp sections were incubated for 2 h in a 5% (v/v) Normal Donkey serum (NDS; Merck) blocking solution in MTSB ...
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bioRxiv - Immunology 2019Quote: ... anti-human C5aR or nonpeptide C5aR antagonist W-54011 (5 μM, Merck Millipore)(25 ...
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bioRxiv - Cancer Biology 2019Quote: ... Sections were then incubated with mouse anti-human mitochondria (clone 113-1, Merck) diluted in blocking solution at a dilution of 1:1000 at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: Immortalized human cerebral microvascular endothelial cell line (hCMEC/D3) was purchased from Merck-Millipore (Darmstadt ...
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bioRxiv - Microbiology 2022Quote: ... microtiter plates coated with either human IgM (30 ng/ml) (Merck, No I8260) for the CP ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-human CD11c (ab216655) underwent buffer exchange using 50kDa amicon filters (Merck) so as to place antibodies in 0.1M NaHCO3 ...
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bioRxiv - Neuroscience 2023Quote: ... The Luminex Milliplex® MAP human cytokine / chemokine magnetic bead panel (HSTCMAG28SK07; Merck) was used to detect cytokines ...