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Citations for Merck :
201 - 250 of 593 citations for Recombinant Human Complement Factor H His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and stained with haematoxylin and eosin (H&E; Merck + Sigma) or IHC using antibodies of interest ...
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bioRxiv - Cell Biology 2023Quote: ... actin stabilizer jasplakinolide (Jasp, 50 nM, 2 h, Merck Millipore) or nuclear export inhibitor leptomycin B (LMB ...
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bioRxiv - Cancer Biology 2023Quote: ... donkey polyclonal anti-rabbit IgG (H+L) (Merck; DUO92002-100RXN), mouse monoclonal anti-rat IgG2b (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... donkey polyclonal anti-rabbit IgG (H+L) (Merck; DUO92005-100RXN), donkey polyclonal anti-rabbit IgG (H+L ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: A549 (human epithelial lung cell line) and EA.hy926 (human endothelial somatic cell line) cells were bought from Merck company and ATCC ...
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bioRxiv - Biochemistry 2023Quote: ... and transiently transfected with the plasmid DNA containing GFP-tagged protein of interest (See Table S7 for plasmid constructs) using GeneJuice transfection reagent (70967, Merck) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Lysates from HEK293 cells overexpressing FLAG-tagged wild typeTP53INP2 or the TP53INP2-3A were affinity-purified with anti-FLAG-M2 affinity beads (Merck). Then ...
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bioRxiv - Molecular Biology 2022Quote: CobB driven deacetylation of His-PrsAc was analyzed by Western blot with anti-acetyl lysine antibodies (Sigma/Merck) and mass spectrometry ...
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Oxidative and non-oxidative active turnover of genomic methylcytosine in distinct pluripotent statesbioRxiv - Cell Biology 2020Quote: ... 10 μg/ml human holo-Transferrin (Merck), 12.5 mg/ml AlbuMAX I (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... and human plasma full-length fibronectin (Merck) was added to the dish at a final concentration of 10 µg/µl for a 1h incubation at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... and human plasma full-length fibronectin (Merck) was added to the dish at a final concentration of 10 μg/μl for a 1h incubation at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... mouse anti-human α5 IgG1 (JBS5; Merck), mouse anti-human α5 IgG3 (P1D6 ...
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bioRxiv - Physiology 2022Quote: ... labelled human mitochondria primary antibody (Merck, MAB1273), 1:500 for 48 h at 4°C and washed with PBS overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... rat anti-human β1 IgG1 (AIIB2; Merck), mouse anti-human β1 IgG1 (Lia1/2 ...
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bioRxiv - Microbiology 2023Quote: ... mouse anti-human α5 IgG3 (P1D6; Merck), mouse IgG1 (JC1 ...
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: ... 180⍰µg/ml Human transferrin (Merck, T8158), 20% D4T Conditioned Media ...
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bioRxiv - Immunology 2023Quote: ... polyclonal anti-human PI3K p85 (Merck Millipore), anti-PI3 Kinase p110α (C73F8 ...
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bioRxiv - Cell Biology 2024Quote: ... or 5µg/cm2 human-plasma fibronectin (Merck) overnight in 4°C ...
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bioRxiv - Immunology 2020Quote: ... Plasma levels of vascular endothelial growth factor (VEGF) was measured using the Milliplex map kit system by Merck Millipore ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 ng/ml basic fibroblast growth factor (bFGF, R&D) and 50 ng/ml Cholera Toxin (Merck-SIGMA). Cells were counted using a haemocytometer ...
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bioRxiv - Neuroscience 2023Quote: ... D-glucose (25 mM, Normapur) and 10 ng/mL of PDGF-AA (Platelet Derived Growth Factor-AA: Merck) and FGF-2 (Fibroblast Growth Factor-2 ...
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bioRxiv - Genomics 2023Quote: ... 1µM PD032552 inhibitors (both from Cell Guidance Systems) and 1000 U/mL units of Leukemia Inhibitory Factor (Merck). Cells were passaged with 0.05% accutase (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... H&E staining was carried out using a kit (Merck, Germany). Muscle fiber cross sectional area was measured with the ImageJ 1.48 software (National Institutes of Health ...
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bioRxiv - Neuroscience 2022Quote: ... H & L Chain Specific Peroxidase Conjugate antibody (Merck Cat# 401315, RRID:AB_2617117). Secondary antibodies for detecting immunoprecipitated proteins are as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 mg/mL hygromycin (H-34274, Merck Millipore, Burlington, MA, USA), and 10 % fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2022Quote: ... and after 44 h with 5 IU of hCG (Ovitrelle, Merck) and then mated with a WT CD-1 male ...
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bioRxiv - Microbiology 2023Quote: ... the H-Tag Antibody HRP Conjugate Kit (Merck-Millipore, Darmstadt, Germany) was used according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... then transiently co-transfected 24 h after seeding using Genejuice (Merck) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... for 3 h or with 100 ng/ml nocodazole (M1404, Merck) for 6 h ...
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bioRxiv - Neuroscience 2023Quote: ... the embryos were cultured for 1 h in KSOM medium (Merck Millipore ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked for 1 h with 5% skim milk (Merck) in TBST [50 mM Tris-HCL ...
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bioRxiv - Cell Biology 2024Quote: ... then transiently co-transfected 24 h after seeding using Genejuice (Merck) according to the manufacturer’s protocol for 24 h ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA tagged with 6xHis at the C-terminus was introduced into a pNG2 vector (a derivative of pET-3a, Merck-Novagen) linearized with NdeI and BamHI ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing the desired His6-tagged protein were concentrated to 2 ml using Amicon® Ultra Centrifugal Filters (10,000 MWCO, Merck Millipore), and were directly injected into a size-exclusion chromatography column (Superdex 75 16/60 ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 hrs the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore). The HeLa cells stably expressing mouse Cx26 were originally obtained from Dr K ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore).
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... rh-HI was prepared in either 20% acetic acid (v/v from acetic acid glacial, Merck KGaA, Darmstadt, Germany), 0.3 M NaCl (Merck KGaA ...
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (10,000 x g, 30 min, RT) and incubated for 1 hour with Ni-NTA His bind resin (Merck) rolling at RT ...
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bioRxiv - Cancer Biology 2019Quote: The uncharged polystyrene 96-well plate were coated with different recombinant proteins-Collagen IV (Merck Millipore), P-Selectin ...
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bioRxiv - Biophysics 2022Quote: ... Both recombinant and synthetic lyophilized peptides were dissolved in 6M guanidine thiocyanate (Merck KGaA, Darmstadt, Germany) and incubated overnight at 1250 rpm ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck, Darmstadt, Germany), upon induction with 0.6□mM IPTG at 0.8 OD600 ...
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bioRxiv - Genetics 2023Quote: ... The screens were performed without feeder cells in M15 medium supplemented with 1000 U/ml leukemia inhibitory factor (Merck). Cells were plated on flasks coated with 0.1% gelatin solution (Merck) ...
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bioRxiv - Cell Biology 2023Quote: ... ESCs were maintained in culture using Dulbecco’s modified Eagle medium (DMEM) with leukemia inhibitory factor (LIF) (1,000 U/ml; Merck-Millipore), L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with F(ab’)2-Goat anti-Human IgG-FITC (Merck Goat anti-Human IgG, F(ab’)2 ...
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bioRxiv - Physiology 2021Quote: Human thrombin was purchased from Merck (Cramlington, UK) and reconstituted in double distilled water (ddH2O) ...
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bioRxiv - Microbiology 2021Quote: ... or polyclonal anti-human antibody (I2136, Merck, Germany) was coupled simultaneously in four flow cells ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-human nuclei (Merck, MAB4383, 1:250) were diluted in the blocking solution and incubated overnight on a shaker at 4◦C ...
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Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... human Ndc80 siRNA (HA12977117-004; Merck, Darmstadt, Germany), human ON-TARGET PRC1 siRNA (L-019491-00-0020 ...