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Citations for Merck :
3851 - 3900 of 5744 citations for Trans Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%;1 D 97% 97% Chemical Purity since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-mouse IgG HRP antibody (AP192P, Merck Millipore). Protein detection was performed using SuperSignal West Pico PLUS (34579 ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-rabbit IgG HRP antibody (AP182P, Merck Millipore) and 1:5000 dilution of mouse anti-GAPDH (ab8245 ...
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bioRxiv - Microbiology 2020Quote: ... NO2- concentrations were determined using colorimetric test strips (NO2--test, 0-10 mgNO2-N·L-1, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Developmental Biology 2021Quote: ... We used the following primary antibodies: rabbit antibody against Protein Kinase Cα diluted at 1:500 (Antibody Registry ID: AB_477345, Merck), rabbit antibody against Recoverin at 1:1000 (AB_2253622 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were resuspended in 10% FCS-PBS again and fixed in 1% paraformaldehyde (PFA) for 10 min at room temperature and reaction quenched with 2.5M glycine (Merck) on ice and centrifuged at 400g for 8min ...
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bioRxiv - Microbiology 2022Quote: Nasal sIgA was obtained from 1 ml nasal swab eluates by centrifugation (1 minute, 10 000 xg) and supernatant was sterile-filtered through a 0.4 μm pore (Merck). We determined sIgA concentrations by ELISA (Human IgA ELISA kit ...
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bioRxiv - Immunology 2022Quote: ... The pH of the solution was adjusted to pH 7.1-7.4 with 1 M NaOH (Merck KGaA, Darmstadt, Germany) and filtered with a silicone- and latex free syringe (#4050-X00V0 ...
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bioRxiv - Neuroscience 2022Quote: Fixed tissue was cut into 1 cm fragments and first impregnated during 3h in a 15%-sucrose solution (Merck) in 1X PBS ...
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bioRxiv - Physiology 2022Quote: ... monoclonal anti-α-actinin (Sarcomeric) antibody produced in mouse (1:1000, Sigma-Aldrich, Merck, clone EA-53, Cat# A7811), at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... were diluted 1:400 v/v in 0.5% low gelling temperature agarose in Ames’ medium at 37°C (A1420; Merck). A thin film was polymerized over a pure agarose meniscus in a Petri dish and covered with Ames’ medium ...
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bioRxiv - Molecular Biology 2022Quote: ... The Lipid II extract was assessed by spotting 1-2 μL on a HPTLC silica gel 60F254 plate (Merck). The TLC plate was developed in a mixture of chloroform:methanol:water:ammonia (88:48:10:1 ...
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bioRxiv - Neuroscience 2022Quote: ... the second step consisted of incubating overnight in PBS-Triton with rabbit anti-Aldh1a1 (1:500, HPA002123, Sigma Merck) and goat anti-calbindin (1:500 ...
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bioRxiv - Plant Biology 2022Quote: ... the CDS of wild type (WT) PpGS1b.1 and PpGS1b.2 were subcloned into pET30a vector (Merck, Darmstadt, Germany) including a N-terminal 6xHis-tag by PCR ...
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bioRxiv - Neuroscience 2023Quote: ... For the example images for survival assay neurons were incubated with chicken polyclonal anti-MAP2 (1:2000; Merck Milipore) and secondary antibody Alexa Fluor 488 ...
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bioRxiv - Biochemistry 2024Quote: ... Diener Electronic) followed by channel surface functionalization using 1% (v/v) trichloro(1H,1H,2H,2H-perfluorooctyl) silane (Merck) in HFE-7500™ (3M™ Novec™).
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bioRxiv - Cell Biology 2024Quote: ... medium—composed of 6.7 g Yeast Nitrogen Base without amino acids (Difco 291940) and 0.8 g complete supplement mixture drop-out (Formedium DCS0019) in 1 L—supplemented with 2% glucose (Merck). Cells from glycerol stocks or agar plates were inoculated into YPD and incubated at 30°C on an orbital shaker at 250 rpm (in an Innova 4230 incubator ...
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bioRxiv - Neuroscience 2024Quote: ... for 180 min prior to washing and development in Streptavidin-tertiary reagent (1:500 in 2% BSA and 0.1% Triton [TBS]) conjugated to either Cyanine 3 (Cy3) (Merck) or Alexa Fluor 488 (Life technologies ...
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bioRxiv - Developmental Biology 2024Quote: mESCs were plated at a final density of 1 × 104 cells/cm2 on fibronectin-coated (MM Merck Millipore, FC010) µ-Slide 8 Well Glass Bottom Chamber Slides (Ibidi ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining of puromycin was performed by incubation with the anti-puro monoclonal antibody (1:1000, Clone MABE343, Merck) for 60 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining of puromycin was performed by incubation with the anti-puro monoclonal antibody (1:1000, Clone MABE343, Merck) for 60 min at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... the membranes were incubated o/n at 4°C in 10 ml washing buffer (1% [w/v] milk powder in TBS-T) containing a 1:10,000 dilution of anti-pan-ADP-ribose binding reagent MABE1016 (Merck) at 4°C48 ...
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bioRxiv - Microbiology 2024Quote: ... Whole cell samples were then analysed by immunoblot using anti-puromycin antibody (1:2000, anti-mouse, clone 12D10, Merck). As a loading control a duplicate SDS-PAGE was performed ...
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bioRxiv - Biochemistry 2024Quote: ... ROS quenching was carried out by treating cells with 5 mM N-acetyl cysteine (NAC) (616-91-1, Merck) for 1 h.
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (10,000 x g, 30 min, RT) and incubated for 1 hour with Ni-NTA His bind resin (Merck) rolling at RT ...
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bioRxiv - Cell Biology 2023Quote: ... 25 mM Tris-HCl, 25 mM NaCl, 1 mM EDTA, pH 7.6, supplemented with 1X protease inhibitor cocktail i.e. PIC, Merck #4693132001). Further ...
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bioRxiv - Physiology 2023Quote: ... Mice were injected intravenously (i.v.) two consecutive days with 1 mg of biotin-X-NHS Ester (BXN) (Millipore, Merck) per mouse dissolved in 200 μl of saline buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The reactions were incubated at 37 °C for 1 h and subsequently stopped by addition of 1x PhosStop (Merck). In vitro phosphorylation of dephosphorylated recombinant SYNJ2BP (0.5 µg in 10 µl ...
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bioRxiv - Molecular Biology 2023Quote: ... The input control was incubated in TE buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA) supplemented with 40 mU/µL proteinase K (#P4850, Merck) for 2 h at 55°C and 1200 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293T cells were transfected at ∼40 % confluency using a 3:1 polyethylenimine (branched, average Mw ∼25,000 Da; Merck) to DNA ratio (30:10 μg ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were blocked with 5% BSA in TSMT (20 mM Tris; 150 mM NaCl, Merck; 1 mM CaCl2 (Sigma); 2 mM MgCl2 ...
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bioRxiv - Genomics 2023Quote: ... Nuclei were then pelleted by centrifugation and lysed in a nuclei lysis buffer (50mM Tris HCl pH8.1, 150mM NaCl, 10mM EDTA, 1% SDS, Complete protease inhibitor cocktail (Merck)) for 20min ...
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bioRxiv - Immunology 2023Quote: ... the mice were intranasally sensitized using 1 μg of 2′3′-cyclic GMP-AMP (cGAMP, 531889; Merck Darmstadt, Germany) and 1 μg of (HDM ...
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bioRxiv - Immunology 2023Quote: ... These oligonucleotides were used to create a phage display library using the T7Select 415-1 Cloning Kit (Merck Millipore). Immunoprecipitation of the library was performed in accordance with a previously published protocol7,11 ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1% DDM, 10% (v/v) ethylene glycol, 0.005% asolectin (Avanti Polar Lipid, USA) and 0.005% CHAPS (Calbiochem, Merck, Germany), and eluted by an increasing proportion of the same buffer plus 1 M NaCl ...
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bioRxiv - Bioengineering 2023Quote: ... Ammonium persulphate ((NH4)2S2O8) 98.0% (APS) and glutaraldehyde (OHC(CH2)3CHO) (Grade 1, 25% in water) were obtained from Merck, Germany ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 24 h at +4°C with primary antibodies (goat anti-Chat (1:200, Merck, AB144P), mouse anti-GFAP (1:300 ...
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bioRxiv - Biochemistry 2023Quote: ... concentration and buffer exchange to TE (10 mM Tris-HCl pH7.5 and 1 mM EDTA) with an Amicon Ultra-4 centrifuge filter unit42 (30 kDa cut-off, Merck). Concentration of all plasmids was measured by NanoDrop One (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... soluble contents of cells were pre-extracted before fixation by incubation for 1 min in 0.1% Triton (T8787-50ML, Merck) diluted in PBS 1X (10010-015 ...
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bioRxiv - Cell Biology 2022Quote: ... MLg mouse lung fibroblasts (CCL-206, ATCC) were proliferation-inactivated by incubation in culture medium containing 10-μg·ml-1 mitomycin C (Merck) for 2 h ...
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bioRxiv - Biophysics 2023Quote: ... Cells were washed trice for 10 min with 0.05% Tergitol in TBS (Tris-Buffered Saline) and blocked for 1 hour with 2% BSA (Merck) in TBS ...
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bioRxiv - Immunology 2023Quote: ... benthamiana was validated by immunoblot analysis using a polyclonal anti-luciferase primary antibody (Merck; used in 1:1000 dilution).
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bioRxiv - Cell Biology 2023Quote: ... specimens were post-fixed for 2 h at RT in 1% osmium tetroxide in 0.05 M cacodylate buffer pH 7.4 (Merck, Germany) and dehydrated stepwise in a graded ethanol series followed by 100% acetone ...
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bioRxiv - Developmental Biology 2023Quote: ... mature pollen was germinated on the surface of solid PGM (18% sucrose, 0.01% H3BO3, 5 mM CaCl2, 5 mM KCl, 1 mM MgSO4 (Merck), pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... 24×50 mm high-precision coverslips (no. 1.5H; Marienfeld) were cleaned in Piranha solution (3:1, 98% H2SO4 (Merck):30% H2O2 (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... coli pellet in 7.5 mL of TBS- N (Tris buffered saline with 0.1% NP-40) and supplemented with 1 x protease inhibitor cocktail tablet (Merck). Cells were sonicated for four rounds of one minute on ...
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bioRxiv - Developmental Biology 2024Quote: ... consisting of 250 μL of HTF and 1 mM reduced L-glutathione (GSH, Sigma-Aldrich-Merck KGaA, Darmstadt, Germany). To reduce the female to female variability ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... tyrosine-hydroxylase (TH) was detected with the primary antibody anti-TH in rabbit (1:600, EMD Millipore - Merck, USA). After washing in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by application of a Cy3-conjugated goat anti-mouse antibody (1:250 dilution, #AP124C; Merck Millipore) as the secondary antibody ...
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bioRxiv - Biophysics 2023Quote: ... Liposomes were labeled before experiments with 1% of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (PE-Rhodamine) (Merck) at a concentration of 10µg/mL.
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bioRxiv - Cell Biology 2023Quote: ... including in an additional experiment where 1 mM of membrane permeable ATP (ATP disodium salt hydrate [A2383 – Merck, Australia]) was added in an attempt to rescue motility ...