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Citations for Merck :
3801 - 3850 of 5744 citations for Trans Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%;1 D 97% 97% Chemical Purity since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Trifluoracetic acid (cat# 1.08262.0025) and Triton X-100 (cat#1.12298.0101) were from Merck KGaA (Darmstadt ...
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bioRxiv - Neuroscience 2024Quote: ... using the Amicon Ultra Centrifugal 100 kDa MWCO Filters (Merck Millipore, Darmstadt, Germany). The filtrates containing 8-OHdG were mixed with the primary antibody and applied to the antigen plate ...
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bioRxiv - Immunology 2022Quote: Lungs were cut into 5 mm3 cubes and digested in 12 ml of collagenase I (2.4 mg/ml, Merck) and DNase I (1 mg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... The starter culture was grown overnight at 37°C 250 rpm shaking and the following day 1 mL was used to inoculate 100 mL of AIM-TB (ForMedium) in a 250 mL baffled shake flask supplemented with 50 μg/mL kanamycin and 0.01% Antifoam-204 (Merck). Cells were grown first for 4 h at 37°C and then for a further 20 h at 18°C using 250 rpm shaking ...
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bioRxiv - Biophysics 2023Quote: 50 µL of 0.8 mg/mL GSK3β-phosphorylated tau fibrils and 0.4 mg/ml of pronase (53702, Merck-Millipore), were incubated in the aggregation assay buffer for 30 minutes at 1,400 rpm shaking in an Eppendorf thermomixer at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: Cells were seeded on glass-coverslips pretreated with poly-L-lysine 50 μg/ml plus laminin 3.3 μg/ml (Sigma-Merck) and fixed with 4% paraformaldehyde before immunolabeling ...
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bioRxiv - Neuroscience 2021Quote: ... coverslips containing fixed neurons were permeabilized and non-specific antigens blocked by incubating in D-PBS with 0.2% Triton-X (Merck, cat. X100-500ML) and 10% donkey serum (Merck ...
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bioRxiv - Neuroscience 2020Quote: ... subjects were given an overdose of sodium pentobarbital (Beuthanasia-D Special, 22 mg/100g body weight, Merck Animal Health, Madison, NJ, USA). Once at surgical plane ...
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bioRxiv - Neuroscience 2022Quote: ... Subjects were given an overdose of sodium pentobarbital (Beuthanasia-D Special, 22 mg/100g body weight, Merck Animal Health, Madison, NJ, USA). Once at surgical plane ...
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bioRxiv - Developmental Biology 2020Quote: ... was performed at room temperature for 1 hour followed by several washing steps and subsequent embedding with Mowiol optionally containing DAPI (#1.24653; Merck).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated for 1.5 hours at RT with an antibody against Hp1α (Merck Millipore, MAB3584, 1:500 dilution) and RNA Polymerase II (Santa Cruz ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated overnight at 4 °C with a monoclonal anti-RBPMS antibody (1:500, Rabbit, ABN1362, Merck Millipore) for the retina ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were washed 6 times for 10min with 1ml wash buffer (10mM HEPES ph 7.3, 150mM NaCl, MgCl2, 10% glycerol, 1% Empigen BB Detergent (Merck), 1x PI) ...
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bioRxiv - Physiology 2020Quote: ... rabbit polyclonal (RP) against phosphorylated Tyr 1077 ObRb (pTyr1077ObR; 1:500, cat# 07-1317, Merck Millipore, Burlington, Vermont, USA), GP against phosphorylated Tyr 1138 ObRb (pTyr1138ObRb ...
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bioRxiv - Microbiology 2019Quote: ... 1 μL of the reaction was spotted onto aluminium backed silica gel plates (5735 silica gel 60 F254, Merck) and dried before being subjected to Thin Layer Chromatography (TLC ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We next centrifuged the mix (1 min, 13,000 g) through a Durapore PVDF 0.22-μm filter (Merck Millipore, US). We injected the CRISPR-Cas9 solution into the male pronucleus of fertilized zygotes with a motor-driven manipulator-based microinjection stage ...
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bioRxiv - Biophysics 2021Quote: ... Myosin II filaments were further diluted in KMEH to 200 nM and added to the actin filaments bound to the SLB by His6-Curly by replacing 1/10 of the sample buffer with the myosin II filament solution and supplemented with 0.1 mM Mg-ATP as well as a mix of 1 mM Trolox (cat. no. 648471, Merck), 2 mM protocatechuic acid (cat ...
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bioRxiv - Biochemistry 2021Quote: ... and soluble protein was clarified by centrifugation (50,000×g, 1 h, 4°C) and subsequently passed through a 0.22-μm filter (Merck Millipore).
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bioRxiv - Neuroscience 2021Quote: The following antibodies were used for immunoassays: primary antibodies - rabbit polyclonal anti-RTN3 (#ABN1723, Merck Millipore; 1:400 dilution), monoclonal anti-NF200 (#N5389 ...
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bioRxiv - Microbiology 2021Quote: DF-1 cells were seeded into a Chambered #1.0 Borosilicate Coverglass slide (Nunc, Lab-Tek, Sigma-Aldrich, Merck, UK) at a density of 8 × 104 per well and cultured in 1ml of DMEM supplemented with 10% hiFBS ...
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bioRxiv - Microbiology 2021Quote: ... which were fixed with methanol for 10 seconds and then stained with Giemsa (1:10 dilution in water; Merck) for 5-10 min prior to microscopy under 100 x magnification with oil.
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bioRxiv - Cell Biology 2021Quote: ... brain sections were subjected to immunohistochemistry using a mouse anti-NeuN antibody (MAB377; Merck, Kenilworth, NJ, USA; 1:500) followed by incubation with an anti-mouse IgG antibody (Vector Laboratories ...
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bioRxiv - Bioengineering 2022Quote: ... samples were fixed in a solution of 4 % (v/v) paraformaldehyde supplemented with 1 % (v/v) glutaraldehyde (Merck, USA) overnight at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then trypsinized and placed in cell culture inserts (1 × 106 cells per insert) with PTFE membrane (Merck) in 6 well plates for overnight cultivation ...
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bioRxiv - Microbiology 2022Quote: ... HRP-conjugated anti-goat or anti-mouse IgG (dilution 1:1,000) were added and protein complexes were visualized using o-phenylenediamine (Merck). The absorbance was then measured at 490 nm employing PowerWave HT (Bio-Tek Instruments ...
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bioRxiv - Cell Biology 2022Quote: ... and were stained on day 6 after the last 1-hour stimulus with dimethyl-methylene blue (DMMB; pH 1.8; Merck) metachromatic dyes as previously described 15 ...
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bioRxiv - Microbiology 2021Quote: Polar metabolites were first extracted from the frozen SHIME-samples by means of ultra pure water (0,055 μS cm-1) obtained via a purified water system (VWR International, Merck). For this purpose ...
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bioRxiv - Microbiology 2020Quote: ... anti-A17L (1:500) in combination with horseradish peroxidase (HRP)-coupled secondary antibodies (1:2,000) and Immoblion Forte Western HRP substrate (Merck) or with IR-Dye secondary antibodies (Licor ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 2 % (vol/ vol) FCS and and 10 mM 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, Merck) and cut into 1 cm pieces ...
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bioRxiv - Immunology 2021Quote: ... Samples were postfixed with 0.1 M sodium cacodylate buffer supplemented with 1 % osmium tetroxide (Science Services) and potassium ferrocyanide (Merck) and embedded in 1 % agarose (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies diluted in 5% milk/PBS-Tween were: mouse anti-Actin (1:2000; Merck, Germany, Cat. No. MAB1501), rat anti-MYC (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: Type 1 diabetes was induced with a single intraperitoneal (i.p.) injection of 150 mg/kg streptozotocin (STZ; Merck, France) freshly dissolved in 0,9 % NaCl ...
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bioRxiv - Microbiology 2022Quote: ... The sections were then incubated overnight in PBS with 0.2% BSA and 0.05% Tween-20 with primary antibodies directed against SARS-CoV-2 Nucleocapsid Protein (1:500; mouse monoclonal, # ZMS1075, Merck); Iba1 (1 ...
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bioRxiv - Cell Biology 2022Quote: ... The sections were then dried and stained on a heating block with a 1% toluidine blue (Merck, 89640-5G) solution including 2% Borax for 90 s at 60°C ...
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bioRxiv - Neuroscience 2019Quote: ... the slices were incubated in the antibody solution (Anti-NG2 Chondroitin Sulfate Proteoglycan, Rabbit, 1:250, AB5320, Merck Millipore) for 48 h at 4 °C ...
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bioRxiv - Physiology 2019Quote: ... semithin sections (1 µm) were used for light microscopy and stained with a solution of 0.5 % toluidine blue O (Merck); ultrathin sections (75 nm ...
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bioRxiv - Physiology 2020Quote: ... Membranes were probed overnight at 4°C with anti-phosphotyrosine antibody (1/1000, 05-321, clone 4G10, Merck Millipore) in blocking solution ...
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bioRxiv - Cell Biology 2019Quote: ... acetylated α-tubulin (Clone 6-11B-1, Cell signalling Technology) or tyrosinated α-tubulin (Clone YL1/2, Merck Millipore) in blocking solution at 4 °C overnight ...
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bioRxiv - Bioengineering 2020Quote: ... labeled for 30 minutes at 4ºC and washed with PBA (PBS with 0.2% sodium azide [Merck]; 1% BSA [Sigma]).DAPI (final concentration of 1 μg/ml ...
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bioRxiv - Biochemistry 2020Quote: Cells were harvested and lysed using RIPA lysis buffer (Beyotime, China) supplemented with 1% protease inhibitor cocktail (MERCK, Germany). After centrifugation at 14 ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Physiology 2019Quote: ... The hPCLS were treated with 10ng/ml recombinant human TGFß 1 protein (R&DSystems#240-B-010) and 25µM (in 750µl of DMEM) of GM6001 (Merck#CC1010). Lived dead staining was carried out using calcein-am/ ethidium homodimer kit (ThermoFisher#L3224 ...
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bioRxiv - Microbiology 2021Quote: ... Isolated lipids were dissolved in 30 μL of chloroform:methanol (2:1, v/v) and 5 μL loaded on thin-layer chromatography (TLC) silica gel plates F254 (Merck). Lipids were separated in chloroform/methanol/water (20:4:0.5 ...
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bioRxiv - Microbiology 2019Quote: ... Reaction products were then visualized by spotting 1 μl on PEI-cellulose thin layer chromatography (TLC) plates (Merck Millipore) and separated using 1 M KH2PO4 ...
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bioRxiv - Genetics 2021Quote: ... a mouse anti-actin monoclonal antibody (#MAB1501R, used at 1:5,000 dilution for the Western blot analyses, Merck Inc.); a mouse anti-glyceraldehyde-3-phosphate dehydrogenase monoclonal antibody (#MAB374 ...
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bioRxiv - Cell Biology 2021Quote: ... Surfaces were functionalised by flooding the device with 1% (v/v) trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Merck) in HFE-7500™ (3M™) ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were passaged at 70 – 90% confluency using 1% PBS and trypsin/EDTA (0.25%,0.02%) in T75 tissue culture flasks (Merck, Australia) and cultured at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... pellets were resuspended in urea lysis buffer (8 M Urea, 50 mM Tris pH 7.5, and 1:200 freshly added protease inhibitor cocktail (Merck)) ...
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bioRxiv - Cell Biology 2020Quote: ... H4S1 phosphorylation was detected using the anti-phospho histone H4/H2A S1p antibody at 1:1000 (Upstate/Merck-Millipore); sf-GFP-Sps1 was detected using JL-8 anti-GFP antibodies (Takara/Clontech ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were washed twice with cytoskeleton buffer and stained with 1:500 30972 Abberior® STAR 635 (Merck) O/N at 4°C ...