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Citations for Merck :
3851 - 3900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... with 1X PIC (cOmplete EDTA-free, Merck), 0.5 μL/ml Benzonase (Merck ...
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bioRxiv - Genomics 2023Quote: ... Western blotting with two additional antibodies was not successful: anti-trimethyl histone H3 lysine 27 (H3K27me3, Merck 07-449) (its 6 a.a ...
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bioRxiv - Microbiology 2023Quote: ... and 1 Protease inhibitor tablet (Merck) per 10 ml of water ...
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bioRxiv - Immunology 2023Quote: ... and dithiothreitol (Merck Millipore) were added to final concentrations of 100 mM and 10 mM ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was filtered through a 0.45 μm low protein binding durapore membrane (Merck Millipore Ltd.), and the progeny virus stock was aliquoted and stored at −20°C until use.
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bioRxiv - Microbiology 2023Quote: ... Fractions corresponding to CVB5 were combined and concentrated to 4.5 mg ml-1 using Amicon Ultra centrifugal filter units with 100 kDa MWCO (Merck Millipore).
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bioRxiv - Microbiology 2023Quote: ... and Fluc (0.05 μg) were transfected using XtremeGENE HP (Merck, Darmstadt, Germany). At 48 h post-transfection ...
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bioRxiv - Immunology 2023Quote: ... Bound antibodies were detected with the horseradish peroxidase (HRP) substrate o-phenylenediamine (Merck KGaA, Darmstadt, Germany). The reactions were stopped with 1 M H2SO4 after 20 min and the absorbance was measured in a microplate reader (Benchmark ...
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bioRxiv - Genomics 2023Quote: ... per 20 μl of protein A/G MagnaBeads (Merck Millipore). Beads were then washed once with low salt wash buffer (0.1% SDS ...
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bioRxiv - Genomics 2023Quote: ... and finally concentrating viral particles on 0.1 μm pore filters (Merck, Millipore (Durapore PVDF Membrane)) ...
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bioRxiv - Immunology 2023Quote: ... 2 and 3 were detected with another multiplex assay (MILLIPLEX MAP TGFß Magnetic Bead 3 Plex Kit; Merck Millipore).
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bioRxiv - Microbiology 2023Quote: ... from a 50 mM biotin stock solution prepared in water with 60 mM NaOH (lyophilized biotin powder, 500 mg stock, Merck Life Science BV (Sigma)) ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant was filtered through Amicon® Ultra – 0.5 mL 10-kDa cutoff centrifugal filters (Merck KgaA; Darmstadt, Germany). To test the enzymatic efficiency of DisA before synthesizing [32P]c-di-AMP ...
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bioRxiv - Microbiology 2023Quote: ... the tetracycline resistance gene from pACE2 (Geneva Biotech, Genève, CH) and the two MCSs from pACYCDuet™-1 (Novagen, Merck Millipore). Csx23 was inserted into MCS-1 of pRATDuet (NcoI/SalI ...
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bioRxiv - Microbiology 2023Quote: ... metoecus cas6f into pCDFDuet™-1 (Novagen, Merck Millipore) as previously described [21] ...
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bioRxiv - Microbiology 2023Quote: ... This plasmid carries the pRSF1030 replicon from RSFDuet™ (Novagen, Merck Millipore), araC and the araBAD promoter from pBAD/His (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... and the flow-through was concentrated using an Amicon Ultracentrifugal filter (3 kDa MWCO, Merck-Millipore) and further purified by gel filtration (HiLoad™ 16/600 Superdex™ 200 gp ...
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bioRxiv - Microbiology 2023Quote: ... The fixed samples were washed with PBS using an Amicon Ultra centrifugal unit (MWCO: 100 kDa, Merck Millipore) and subjected to size exclusion chromatography to further purify the viral fraction as described elsewhere59 ...
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bioRxiv - Genomics 2023Quote: ... cells were detached with Accutase (Merck Life Sciences) and 3 x 106 cells were plated to grow in suspension in 90 mm bacterial petri dishes containing N2B27 media supplemented with 1 μg/ml doxycycline and 10 ng/ml EGF and FGF (Peprotech) ...
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bioRxiv - Microbiology 2023Quote: ... Csx23-containing fractions were concentrated using an Amicon Ultracentrifugal filter (30 kDa MWCO, Merck-Millipore) and further purified by gel filtration (HiLoad™ 16/600 Superdex™ 200 gp ...
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bioRxiv - Immunology 2023Quote: ... The eluted His-Myc tagged recombinants were further concentrated using 3kDa ultra concentration units (Merck, Germany). The GST-tagged recombinant proteins were affinity purified using Glutathione Sepharose 4B beads and purified in batch mode ...
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bioRxiv - Immunology 2023Quote: ... the slides were dehydrated in an increasing alcohol gradient and finally the samples were mounted using DPX (Merck). Positive cells have a brown staining indicative of the precipitate of the DAB substrate ...
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bioRxiv - Immunology 2023Quote: ... after the addition of deuterated lipid standard (Splash Lipidomix®, Merck, Berlin). The samples were vortexed and sonicated for 2 min and then incubated for 30 min at 4°C under gentle and constant shaking ...
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bioRxiv - Immunology 2023Quote: ... Lungs were digested in Worthington collagenase III (Scimar, AUS) supplemented with DNase I (Merck, AUS) and all tissues transferred through a 0.70 μM cell strainer (Corning ...
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bioRxiv - Immunology 2023Quote: ... Pepstatin A Methyl Ester (Pepstatin A, 516485) and MCC950 (256373-96-3) were purchased from Merck. Ultrapure™ DNase/RNase-Free Distilled Water (10977035 ...
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bioRxiv - Immunology 2023Quote: ... Cell supernatants were centrifuged at 500 ×g for 5 min to remove dead cells and concentrated with 10 kDa MW cut-off filters (Amicon, Merck Millipore), as described by the manufacturer ...
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bioRxiv - Immunology 2023Quote: Bovine cytokines and chemokines secreted in the supernatant by cells were measured using the commercial multiplex immunoassay MILLIPLEX MAP Bovine Cytokine/Chemokine Panel 1 (Merck/Sigma). Therefore ...
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bioRxiv - Immunology 2023Quote: ... The Fmoc-derivatized amino acid monomers and the preloaded resins used as support were obtained from Merck. Once synthesized the peptides were cleaved from the resin with a standard scavenger-containing trifluoroacetic acid (TFA)-water cleavage solution and precipitated by addition to cold ether ...
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bioRxiv - Immunology 2023Quote: ... for 15min and incubated with propidium iodide (40 μg/mL in PBS) and RNase A (100units/mL, cat. no. 10109142001, Merck) for 30min at 37[ ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The residues in the vicinity (5.0 Å) of the small molecule CCX662 were optimized for the hydrogen bond network and minimized (cartesian minimization in Merck molecular force field, MMFF). VUF15485 was docked and scored using the ICM VLS scoring function (Neves et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... After labelling nuclei with Hoechst 33342 (Cat# B2261, Merck) for 5 min at RT ...
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bioRxiv - Immunology 2023Quote: ... ELISpot PVDF plates (Merck, MSIPS4W10) were activated using 15 μL/well of 35% ethanol ...
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bioRxiv - Immunology 2023Quote: ... 100 μL/well of TMB (Merck, T0565) was added to develop until distinct spots emerged ...
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bioRxiv - Neuroscience 2023Quote: ... adhered to a poly-L-lysine (Merck) coated coverglass ...
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bioRxiv - Neuroscience 2023Quote: ... fixed and stored organoids were saturated with 30% sucrose (Merck), embedded in O.C.T ...
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bioRxiv - Neuroscience 2023Quote: ... in phosphate-buffered saline (PBS) and subsequently blocked in a solution containing 2% normal goat serum (Merck) within the same permeabilization solution ...
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bioRxiv - Neuroscience 2023Quote: ... histological crysections of the cerebral organoids were initially permeabilized using a 0.2% Triton-X solution (Merck) in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2023Quote: Picrotoxin (Merck, NJ, USA) was prepared to 25mg/ ml in 100% ethanol ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 1% acetic acid for 30 seconds and Safranin’O solution for 45 min (S2255, Merck). For immunofluorescence ...
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bioRxiv - Neuroscience 2023Quote: ... then resuspended in a mixture of protease inhibitors (Merck, Whitehouse Station, NJ). The cell suspension was treated with 0.1% lysozyme ...
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bioRxiv - Molecular Biology 2023Quote: ... All chemicals were purchased from Merck.
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bioRxiv - Molecular Biology 2023Quote: ... and 1 µl of each sample was spotted on polyethylenimine (PEI) cellulose thin-layer chromatography plates (Merck) and developed in 0.75 m LiCl.
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% NP-40 and protease inhibitor cocktail (complete Mini, Merck, Sigma Aldrich). Cells were lysed for 10 min incubation on ice and lysates were cleared with a 15 min centrifugation at 12000 g and 4°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... were dissolved in bovine serum albumin (BSA, Merck Life Science UK Ltd ...
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bioRxiv - Synthetic Biology 2023Quote: ... RNA was extracted from the microplate using the RNAspin Mini Kit (Merck; GE25-0500-71) and quantified using a NanoDrop™ Spectrophotometer (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... anti-fibronectin (AB1945, Merck); anti-integrin α5 (sc-166665) ...
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bioRxiv - Microbiology 2023Quote: Mycobacterium ulcerans strain Mu_1082 was cultivated on Middlebrook 7H11 agar (Merck) supplemented with 0.2% glycerol (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... TRITC-conjugated phalloidin (FAK100, Merck), Alexa Fluor 594 goat anti-rabbit (A11012) ...
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bioRxiv - Microbiology 2023Quote: ... were performed on HUVECs at 30-40% confluency using Escort IV transfection reagent (L3287, Merck). After 48 hours ...
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bioRxiv - Microbiology 2023Quote: ... used either Immobilon PVDF membranes (Merck) or nitrocellulose membrane (GE Healthcare) ...