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Citations for Merck :
3551 - 3600 of 5744 citations for Trans Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%;1 D 97% 97% Chemical Purity since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was acidified with 100% formic acid (#1.00264.0100, Merck, DK). C18 stage tips (UltraMicroSpin Column ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 10 min permeabilization with 0.1% TritonX-100 (Merck, X100-100ML) before blocking with 100μL blocking buffer from the kit for 1h ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... with 100 μl of MS pure n-hexane (Merck KGaA, Darmstadt, Germany). The vials were then put on an orbital shaker (KS 130 basic ...
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bioRxiv - Cell Biology 2023Quote: ... followed by permeabilization in 0.5% Triton-X-100/PBS (Merck/Millipore #1086031000) for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 μL of protein RIPA lysis buffer (The Merck Group, Darmstadt, Germany) with proteinase (Halt™ Protease ...
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bioRxiv - Molecular Biology 2023Quote: ... concentrated using 100 kDa Amicon® Ultra-15 Centrifugal Filter Units (Merck) and cryopreserved at –80°C ...
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bioRxiv - Biophysics 2022Quote: ... 100 μl of 50 mM Cysteine (L-Cysteine for biochemistry from Merck) and 200 μl of (in 10 μg/mg mAb concentration ...
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bioRxiv - Genetics 2023Quote: ... 0,5% Triton X-100 and cOmplete™ Protease Inhibitor Cocktail (Merck # 11697498001])) and lysed with zirconium beads (Lysing Matrix Y ...
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bioRxiv - Neuroscience 2023Quote: a low cAMP signal (50-100 μM dibutryl-cAMP (dbcAMP; Merck - D0627) or 2
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bioRxiv - Cell Biology 2023Quote: ... fixed cells were permeabilized with 0.1% Triton X-100 (Merck, cat #T8787) in D-PBS supplemented with 1% BSA (Merck ...
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bioRxiv - Physiology 2024Quote: ... and blocked and permeabilized with a 0.2% Triton X-100 (Merck T8787) - 3% horse serum (Merck ...
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bioRxiv - Immunology 2024Quote: ... permeabilized for 5 min using 0.1% vol/vol Triton X-100 (Merck), and blocked using 5% vol/vol FCS ...
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bioRxiv - Biophysics 2024Quote: ... A final purification step using Amicon 100 k spin columns (Merck, #UFC510024) was used to remove unbound oligonucleotides ...
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bioRxiv - Neuroscience 2024Quote: ... After three 15-minute washes in PBST (0.3% Triton X-100, Merck Sigma-Aldrich X100 ...
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bioRxiv - Molecular Biology 2024Quote: ... Permeabilization step was performed with 0.1% Triton X-100 (Merck, #X100-100ML) in PBS for 10min at room temperature before blocking 1h at 37°C with Duolink Blocking buffer ...
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bioRxiv - Plant Biology 2020Quote: ... 0.1 g of the ground material was digested overnight at 90°C in a mixture of 2 ml HNO3 and 0.5 ml H2O2 (30% Suprapur, 1.07298.1000 Merck). Then 50 μl of HF (40% reagent grade ...
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bioRxiv - Biochemistry 2022Quote: ... Amicon ultra 15 ml 10 kDa (#UFC901008) and ultra 0.5 ml 10 kDa (#UFC501008) concentrators from Merck; Superose 6 (10/300 ...
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bioRxiv - Microbiology 2022Quote: ... 5 mg/ml Hemin (Mercury Cat#3741) and 5ul/ml vitamin K1 (Merck Cas#84-80-0)) under anaerobic conditions.
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bioRxiv - Microbiology 2023Quote: ... the second 500mL culture was supplemented with kasugamycin 40mg/mL (final concentration = 250μl/mL) (Merck, #32354-100MG) and the third culture was supplemented with the equivalent volume of water (no antibiotic) ...
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bioRxiv - Cell Biology 2019Quote: ... 6 ml deionized water, 12 ml 0.2 M Tris buffer pH 8.5, 2.4 g Mowiol 4–88, Merck Millipore). The staining of BCRs of fixed Ramos cells ...
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bioRxiv - Developmental Biology 2019Quote: ... The viral supernatant was mixed with 8 μg/ml DEAE-dextran and 1000 units/ml ESGRO (Merck Millipore) and added directly to the E14tg2a cells ...
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bioRxiv - Immunology 2023Quote: ... Fractions corresponding to immune complexes (∼9.8 mL–11.2 mL) were pooled and concentrated using a 100,000 MWCO centrifugal filter (Merck Millipore). To generate immune complexes for cryo-EM where UltrAuFoil R1.2/1.3 grids were used and consequentially a higher concentration of immune complex was required ...
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bioRxiv - Immunology 2022Quote: ... then digested with Liberase Blendzyme TM at 1.1 U/ml and Dnase I at 40 μg/ml (Merck) in HBSS under constant shaking at 37°C for 40 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... 5 µg/mL leupeptin) supplemented with 250 µg/mL lysozyme (from hen egg white; Merck KGaA, Darmstadt, Germany) and incubated on ice for 30 minutes ...
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bioRxiv - Biophysics 2024Quote: ... The sample was then loaded again on a 5 mL His-Trap Fast Flow column and the flow-through was concentrated down to 0.5 mL using a 15 mL Amicon centrifugal filter with a 3.5 kDa cutoff (Merck). The sample was then loaded on a Superdex 75 Increase 10/300 GL column mounted in the same chromatography system and equilibrated in NMR buffer (20 mM sodium phosphate ...
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bioRxiv - Immunology 2021Quote: ... Mouse peritoneal macrophages (MPMs) were isolated from mice 4 d after the intraperitoneal injection of thioglycollate (Merck & Co.; Kenilworth, NJ, USA) as described previously(Zhang et al. ...
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bioRxiv - Neuroscience 2022Quote: ... The virus-containing fraction was extracted from the tubes and AAVs were then concentrated in Dulbecco’s Phosphate Buffered Saline (D-PBS) + 5% sucrose using Amicon Ultra-15 (100K) filter units (Merck Millipore UFC910024) at 3220 g ...
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bioRxiv - Genetics 2023Quote: Wild type and PREPL KO HEK293T cells were grown on glass coverslips coated with Poly-D-Lysine (Merck A-003-E) and fixed with 4% formaldehyde ...
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bioRxiv - Evolutionary Biology 2024Quote: ... rinsed once again with PBS-d and then proceeded to blocking using 5% of NGS (normal goat serum, G9023, MERCK, Germany) in PBS-d for 2-4 hours ...
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bioRxiv - Cell Biology 2020Quote: ... in Biotin binding buffer (5 mM Tris-HCl pH 7.5 [Sigma], 0.5 mM EDTA [AppliChem], 1 M NaCl [Merck]) for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.0) supplemented 5 mM benzamidine (Carl Roth), 0.1 mM AEBSF (AppliChem, Darmstadt, Germany) and Benzonase (1:1000, Merck) for 1 h at 4°C on a wheel rotator ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed and blocked as per western blotting protocol and probed with 1:1500 BG4 (Merck Millipore), 1:1500 DYKDDDK tag (anti-flag ...
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bioRxiv - Developmental Biology 2020Quote: ... The gels were subsequently demulsified with 45 µl 1H,1H,2H,2H Perfluoro 1 octanol (PFO) (Merck, #370533) into 200μl of MM+ Ri medium ...
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bioRxiv - Molecular Biology 2020Quote: ... the permeabilized cells were incubated for 1 hour with anti-phospho-S10 Histone H3 (06-570, Merck Millipore) in PBS containing 0.1% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... were used at a 1/10000 dilution before reaction with Immobilon Forte Western HRP substrate (WBLUF0100, Merck Millipore).
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bioRxiv - Bioengineering 2019Quote: ... Samples were post-fixed for 1h in a 1% osmium tetroxide solution in 0.1M sodium cacodylate buffer (Merck) and rinsed three times in the same buffer ...
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bioRxiv - Neuroscience 2019Quote: ... cells were fixed with 4 % paraformaldehyde and stained for doublecortin (guinea pig anti-DCX IgG, 1:400, Merck Millipore followed by Alexa 488 anti-mouse ...
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bioRxiv - Evolutionary Biology 2019Quote: All experiments were carried out in casamino acids medium (CAA) containing 5 gl-1 casamino acids (Merck, Switzerland), 1.18 g l−1 K2HPO4*3H2O ...
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bioRxiv - Biochemistry 2020Quote: TSEN complexes were mixed to a final concentration of 1 or 3 μM with 4x SYPRO Orange (Merck) stock in 50 mM HEPES-NaOH ...
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bioRxiv - Microbiology 2021Quote: ... To ensure sterility 1/10 of the inactivated material was incubated in thioglycolate-medium (Merck KGaA, Darmstadt, Germany) for seven days without growth before material was taken out of the BSL3 laboratory.
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bioRxiv - Biochemistry 2020Quote: ... anti-RNA polymerase II subunit B1 (phospho CTD Ser-2) clone 3E10 (Merck, 04-1571-I, 1:5,000), and anti-RNA polymerase II CTD repeat YSPTSPS antibody clone 8WG16 (Abcam ...
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bioRxiv - Bioengineering 2021Quote: INS-1 cells were stained with red fluorescent membrane-inserting dye PKH-26 (Lot# SLBW0232, Merck, Darmstadt, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and mouse monoclonal anti-APP (1:500, 22C11; Cat#Mab348, RRID: AB_94882, Merck Millipore/Sigma, Burlington, MA, USA) antibodies were purchased from the indicated suppliers ...
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bioRxiv - Neuroscience 2021Quote: ... brain slices of mice were also incubated with a mouse NeuN (1:500, conjugated with AF488 MAB377X, Merck) and a rabbit GFAP antibody (1:1000 ab7260 ...
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bioRxiv - Plant Biology 2021Quote: ... dried and placed in a ten by ten grid pattern on square culture dishes containing 45 ml of nitrogen replete (+N) (0.5x Murashige and Skoog salts [Caisson Labs], 1% sucrose, 0.5 g/L MES and 0.4% Phytagel [Merck Sigma-Aldrich] ...
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bioRxiv - Microbiology 2020Quote: ... The following secondary antibodies were used: sheep α-rabbit horseradish peroxidase (HRP) (catalogue number AP510P, 1/10,000; Merck) and goat α-mouse HRP (STAR117P ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EGTA and protease inhibitors) on an Amicon 30 kDa cut off concentrator (Merck, Cat# UFC 503096). Protein concentration was determined using a BCA protein assay (Thermo Scientific ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM EDTA pH 7.2 with protease inhibitor tablets (cOmplete™, EDTA-free protease inhibitor cocktail, Merck, UK)) and sonicated 30 s on and 30 s off for three rounds using a XL-2020 sonicator (Heat Systems ...
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bioRxiv - Cell Biology 2020Quote: ... Proteasome complexes were subsequently blotted for 1 h at 200 V on ice onto PVDF membranes (Merck Millipore) and further handled as described above.
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bioRxiv - Biochemistry 2022Quote: ... the coding sequence was cut out from the pFastBac 1 construct and transferred to pET28a (Merck, Darmstadt, Germany) by means of HindIII and Eco53kI ...