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Citations for Merck :
3451 - 3500 of 3899 citations for 1 1 Ethynylcyclohexyl azetidin 1 ium 3 yl n naphthalen 1 ylcarbamatechloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... proteins were subsequently alkylated with 20 mM Indole-3-acetic acid (Merck) for 1h at room temperature in the dark and diluted to 2M Urea ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Genetics 2023Quote: Chemical extractions of single wasps were performed by immersing them in 50 µl HPLC-grade n-hexane (Merck, KGaA, Darmstadt, Germany) in 2 ml glass vials (Agilent Technologies ...
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bioRxiv - Biochemistry 2023Quote: The SUN domain sequence of human SUN1 (amino-acids 616-812) was fused to a TEV-cleavable N-terminal His6-GCN4 tag and cloned into a pRSF-Duet1 vector (Merck Millipore). The KASH domain sequence of human JAW1/LRMP (amino-acids 515-555 and 531-555 for KASH6 and KASH6 core ...
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bioRxiv - Cancer Biology 2024Quote: ... a cassette coding for tdTomato with an N-terminal nuclear localization sequence and lack of a stop codon and a puromycin cassette with an N-terminal self-cleaving T2A peptide were amplified by KOD polymerase (Merck, 71085) and subjected to Gibson assembly.
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bioRxiv - Neuroscience 2024Quote: ... IL-10 and TNF-α were quantified in the FN of NL and at 21dpi aged GFAP-IL6Tg and WT mice (n= 4-6/experimental group) using a Milliplex MAP Mouse High Sensitivity kit (#MHSTCMAG-70K; Merck Millipore), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Purified Mpro with authentic N- and C-termini was concentrated by a 10 kDa MWCO Amicon Ultra 50 centrifugal filters (Merck Millipore) at 4 °C to ∼ 10 mg/ml ...
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bioRxiv - Plant Biology 2024Quote: The presence of T-DNA in transgenic plants was confirmed via extraction of genomic DNA and genotyping by PCR with the REDExtract-N-Amp™ Plant PCR-Kit (Merck, Darmstadt ...
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The node of Ranvier influences the in vivo axonal transport of mitochondria and signalling endosomesbioRxiv - Neuroscience 2024Quote: ... with 7.5-10 μg of HCT in PBS in a volume of ∼3.5 µl using a 701 N Hamilton® syringe (Merck, 20,779) for TA ...
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bioRxiv - Biochemistry 2024Quote: ... The activity assay was performed by incubating 0.5 nM PT5 with 100 μM chromogenic substrate N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide (100 μM; Merck, Darmstadt, Germany) and two-fold macrocyclic peptide dilutions ranging from 0.0005 to 2000 nM ...
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bioRxiv - Cancer Biology 2024Quote: PC4 cDNAs wild type or carrying the W89A mutation and with a N-terminal 6x His-tag sequence were cloned into a pET-Duet1 plasmid (Merck Millipore) and transformed into competent BL21 cells (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... It was inserted with N and C-terminal tags (His6 and StrepII, respectively) in the baculovirus transfer vector pIEX/Bac3 (Merck Novagen #717263).
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bioRxiv - Pharmacology and Toxicology 2023Quote: CC strips were dissected and then transferred to microtiter plate wells containing 5 μM bis-N-methylacridinium nitrate (Lucigenin; Merck, Darmstadt, Germany), some strips were stimulated with NADPH (100 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissues were directly immersed in CUBIC-L solution (10 wt % N-Butyldiethanolamine [Tokyo Chemical Industry, B0725], 10 wt % Triton X-100 [Merck, 1.08603.1000] and 80 wt % MilliQ water) ...
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bioRxiv - Plant Biology 2023Quote: ... Pulldowns were done as described17 with the following modifications: The biotinylated Aux/IAA7 DII peptide (AKAQVVGWPPVRNYRKN, N-terminal biotin) was bound to streptavidin-agarose beads (Merck, #16-126). 250 μl of total protein extract was combined with 45 μl of DII-beads and either 0 μM or 10 μM cvxIAA or 10 μM IAA ...
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bioRxiv - Biophysics 2024Quote: ... and H3.1 were subcloned into a pET15b-derived plasmid containing an N-terminal 6×His tag (MGSSHHHHHHSSG) in which the thrombin cleavage site of pET15b (Merck Millipore, 69661) was replaced by the TEV protease recognition sequence ...
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bioRxiv - Developmental Biology 2024Quote: ... ten E6 morulae were transferred to each recipient cows (n = 2) following synchronization with initial intramuscular injection of gonadotropin-releasing hormone (Fertagyl; Merck, Rahway, NJ), standard 7-day vaginal controlled internal drug release (EAZI-BREED CIDR ...
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bioRxiv - Biochemistry 2020Quote: ... RNAs were concentrated by ultrafiltration using Amicon Ultra 3 MWCO centrifugal filters (Merck) and stored at −20 °C ...
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bioRxiv - Biophysics 2021Quote: ... The fractions containing ubiquitin were concentrated by Amicon Ultra-15 3 kDa (Merck) and then loaded onto a HiLoad 16/60 Superdex 75 column (GE Healthcare Life Science ...
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bioRxiv - Plant Biology 2020Quote: ... with 3 kDa molecular weight cut off (Amicon® Ultra, Merck Millipore Ltd.) to remove high molecular weight conjugates of IAA ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was estimated using BCA protein assay kit (Merck, 71285-3, Germany).
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bioRxiv - Immunology 2022Quote: ... MCP-3 and RANTES were quantified using a multiplex kit (MILLIPLEX MAP; Merck, Burlington ...
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bioRxiv - Immunology 2021Quote: ... Peritoneal macrophages were isolated from mice 3 days after injection of thioglycollate (MERCK) and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2022Quote: ... RNAs were concentrated by ultrafiltration using Amicon Ultra 3 MWCO centrifugal filters (Merck) and stored at −80 °C ...
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bioRxiv - Pathology 2020Quote: ... Non-specific binding sites were blocked via incubation in 3% H2O2 (Merck, Germany) and BSA respectively ...
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bioRxiv - Neuroscience 2020Quote: ... reverse: 5’-CCAGGGTGGAGCGGTC-3’) and the KOD Hot Start Mastermix (Merck, Darmstadt, Germany). The plasmids were confirmed by sequencing (Seqlab ...
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bioRxiv - Developmental Biology 2021Quote: ... washed 3 times in PBS with 1x cOmplete Protease Inhibitor Cocktail (Merck, 11697498001).
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bioRxiv - Biochemistry 2023Quote: ... PAPS (adenosine 3′-phosphate 5′-phosphosulfate, lithium salt hydrate) was purchased from Merck and stored at -80 °C to afford maximal stability.
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bioRxiv - Plant Biology 2023Quote: ... and the pH was adjusted to 5.5 with KOH (1310-58-3, Merck). The plates containing the seeds were stratified for 2 days at 4°C in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... The eluate was concentrated with Amicon Ultra-15 (Merck Millipore, MWCO 3 K) to a protein concentration of 60 µM.
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bioRxiv - Systems Biology 2022Quote: ... 10+3) were seeded onto fibronectin coated plates (20 µg/mL, FC010 Merck) at a density of 0.25 x 106 cells/cm2 and cultured for three (d3 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) was acquired from Merck (Darmstadt, Germany). LNP were produced using microfluidic mixing with the Dolomite Microfluidics system (Dolomite Microfluidics ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore) at 3500 rpm and 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... followed by an Amicon ®Ultra centrifugation filter (3 kDa cut-off, Merck). The sample was transferred to a 5 mm NMR-tube (Labscape™ Essence ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples was concentrated by 3 kDa Amicon Ultra centrifugal filters (Merck Millipore, R0NB30416) with 100 mM NH4HCO3 to exchange the buffer and retain globular particles above 3 kDa ...
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bioRxiv - Immunology 2023Quote: ... Tissue slides were blocked in 1X PBS with a 3% BSA (Merck, A7906), 10% Donkey serum (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... The slides were blocked overnight in PBS solution containing 3% BSA (Merck, #A7906), 10% donkey serum (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... 4°C with 3 K Amicon® Ultra-15 centrifugal filters (Merck Millipore). All fractions were stored as aliquots at − 80 °C for later use ...
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bioRxiv - Microbiology 2024Quote: ... 3 μl of the resuspended cells were spotted on LB (0.2% starch (Merck)) and were incubated 24h at 30°C ...
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bioRxiv - Microbiology 2024Quote: ... They were washed 3 times with HBSS (Merck Sigma, Saint Quentin Fallavier, France) before adding 500 µl RPMIc for a further 24 h incubation before the interaction with bacteria.
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bioRxiv - Synthetic Biology 2024Quote: ... 49.37 mM sodium succinate dibasic (Merck/Sigma-Aldrich, CAS number : 150-90-3). Final amino acids and nucleic bases concentrations used are based on the EZ rich defined medium composition63 ...
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bioRxiv - Genetics 2023Quote: ... were obtained by calibrating each compound according to a dilution series based on the closest eluting n-alkane from a C21-40 standard series (Merck, KGaA, Darmstadt, Germany) at 0.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The Mpro fractions were concentrated using 3 kDa Amicon Ultra Centrifugal Filters (Merck Millipore) and the Mpro protein was further purified by size exclusion chromatography using a HiLoad Superdex 200 column (Cytiva ...
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bioRxiv - Neuroscience 2020Quote: ... the plate was blocked with blocking buffer (3% BSA (Merck Millipore, MA, USA 820451) in PBS ...
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bioRxiv - Microbiology 2020Quote: ... 13 mM glycine and 3 μM thiamine (all compounds from Merck Millipore, Darmstadt, Germany).
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bioRxiv - Biophysics 2022Quote: ... cells were incubated with BioTracker 609 Red Ca2+ AM dye (3 μM, Merck Millipore) in fresh medium for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... with a Chromolith® RP-18 endcapped 5-3 guard cartridges (Merck, Darmstadt, Germany), operated under a flow rate of 0.3 mL/min ...
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bioRxiv - Biophysics 2022Quote: ... Free dye was removed using a 3 kDa cutoff Amicon centrifugal concentrator (Merck, NJ), and the buffer was exchanged to 50 mM sodium phosphate ...
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bioRxiv - Biochemistry 2021Quote: ... and the protein was concentrated using Amicon 3 kDa MWCO centrifugal filters (Merck Millipore). The protein concentration was calculated using the measured absorbance at 280 nm under native conditions and assuming an extinction coefficient of 19940 M-1 cm-1.