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Citations for Merck :
301 - 350 of 1379 citations for Recombinant Human S100 Calcium Binding Protein A9 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Lysate was clarified by spinning cells for 10 min at 20,000 × g before loading supernatant onto cOmplete™ His-Tag purification resin (Merck; cat # 5893682001). Bound proteins were washed with 20 mM tris (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... The 100-fold dilution of culture was made up to 10-4 dilutions and spotted on TYE plates (Hi-Media Laboratories Ltd., India) having 1% glucose (Merck & Co., USA) and ampicillin (Gold Biotechnology ...
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bioRxiv - Biochemistry 2021Quote: ... protein standards (Merck Sigma-Aldrich) specified in the Supplementary Material were separated on the same column.
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bioRxiv - Microbiology 2023Quote: ... protein A sepharose beads (Merck) were incubated with the cleared supernatant for an hour to remove non-specific binding ...
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bioRxiv - Pathology 2022Quote: ... The elution fraction containing His6-PhoP was dialyzed in Binding Buffer to remove the high concentration of imidazole and concentrated using an ultrafilter (Merck KGaA, Darmstadt, Germany). SDS-PAGE and Western blot with an anti-His tag antibody was used to confirm the purified protein (ABclonal ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were seeded in culture dishes and the specific pharmacological inhibitors radicicol (inhibitor of ATP-binding site of Hsp90, Sigma-Aldrich, Merck, Darmstadt, Germany), cyclosporine A (inhibitor of Cyp activity ...
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bioRxiv - Cancer Biology 2023Quote: ... 250 ng of pTOPflash (wt-TCF) or pFOPflash (mutated-TCF binding site, negative control) reporter plasmids (TOPflash luciferase reporter assay, Merck Millipore, Darmstadt, Germany) were transfected with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... and then clarified via passing through Millex® -GP Fast Flow & Low Binding Millipore Express® PES Membrane 0.22 µm syringe filter unit (SLGP033RS, Merck Millipore, USA).
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bioRxiv - Immunology 2021Quote: ... Human pancreatic beta cell line 1.4E7 was purchased from Merck and cultured in RPMI-1640 medium (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... Part B was made of 10U/mL human thrombin (Merck) in DMEM/F12 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and purified human C3a was purchased from Merck (Perth, Australia). Recombinant human C5a (rhC5a ...
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bioRxiv - Immunology 2020Quote: ... FITC conjugated goat anti-human IgG Fc was from Merck, Dorset ...
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bioRxiv - Microbiology 2023Quote: ... sensitivity assays against human serum (Merck Millipore, Burlington, MA, USA), colistin (Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... After incubation with FcBlock (PBS + 12% human serum AB (Merck)) for 10 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... The human cardiomyocyte cell line AC16 was purchased from Merck Millipore (Burlington ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... and HRP-conjugated goat anti-human IgG (AP309P, Merck KGaA). Protein detection was done with enhanced chemiluminescence (ECL ...
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bioRxiv - Cell Biology 2024Quote: ... coated with 50 µg/ml human serum fibronectin (Merck, Germany). Imaging was performed on a Nikon Widefield Ti2 equipped with a sCMOS ...
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bioRxiv - Biophysics 2020Quote: ... beads were removed and recombinant dynein was concentrated in a 100 KDa cut-off filter (Amicon Ultracel, Merck-Millipore) to a final volume of 500 μL ...
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bioRxiv - Microbiology 2023Quote: ... were incubated for 2 h at room temperature with the respective recombinant glycosyltransferase enzymes (6.3 µg/ mL) and UDP-GlcNAc (2 mM, Merck) in glycosylation buffer (15 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... protein lysates were cleared by centrifugation and protein concentration was measured using Coomassie reagent (Merck). Denatured ...
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bioRxiv - Bioengineering 2021Quote: Three sender area squares (3 × 3 mm) were put into a 24-well containing 300 μL of anti-His antibody (Merck, Germany, NOVG70796-3) (10 μg mL−1 in DPBS ...
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bioRxiv - Cancer Biology 2021Quote: Primary HPMECs in EBM2 medium or ST1.6R cells in DMEM/1% FBS were cultured overnight and stimulated with 2 μg/ml of recombinant TNC (Merck Millipore) for 24 h ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... The dose of COS (75-400 IU daily of recombinant follicle stimulating hormone (Gonal-F Merck or hMG Menopur, Ferring) was individualized to patient’s age ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... Ovarian follicle growth tracking was performed by scheduled transvaginal ultrasound examinations that lead to the planning timepoint for oocyte maturation triggered by administration of recombinant hCG (250 microgr SC Ovitrelle; Merck). Oocyte retrieval was carried out by transvaginal ultrasographically-guided follicular puncture 37 hours later.
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bioRxiv - Physiology 2022Quote: ... To detect total protein carbonylation by immunoblot we used the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore ...
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bioRxiv - Cell Biology 2021Quote: Human immortalised articular chondrocyte C28/I2 cells were purchased from Merck Millipore and cultured in DMEM/F12 (Gibco ...
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bioRxiv - Cell Biology 2022Quote: The human brain microvascular endothelial cell line hCMEC/D3 (Merck Millipore) was seeded in T25 flasks coated with rat tail type I collagen (Merck Millipore) ...
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bioRxiv - Cancer Biology 2020Quote: ... When stained for human nuclei (MAB1281, Merck Millipore, Fig. 4C-D), this was performed for 30 min at RT in the dark after EdU staining (Click-iT Assay ...
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bioRxiv - Microbiology 2020Quote: ... and affinity-purified rabbit polyclonal antibody against human CPSF6 (HPA039973; Merck) were used at a dilution of 1:1,000 ...
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bioRxiv - Neuroscience 2022Quote: The HCYTOMAG-60K-15 Human Cytokine/Chemokine Multiplex Assay (Merck Millipore) was employed to simultaneously analyze TNF-α ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 IU mL1 human chorionic gonadotropin (hCG; Chorulon; Merck Animal Health) and 1.0 mg mL1 oestradiol ...
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bioRxiv - Immunology 2023Quote: ... and human rIL-2 (30 U/ml) (Roche/Merck, Darmstadt, Germany) to the culture ...
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bioRxiv - Developmental Biology 2023Quote: ... Coating was performed with 20 µg/ml human fibronectin (Merck FC010) in PBS for at least 30’ ...
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Lowering mutant huntingtin by small molecules relieves Huntington’s disease symptoms and progressionbioRxiv - Molecular Biology 2023Quote: ... and anti-eIF4G1 for human samples (1:2000; 07-1800, Merck) or anti-eIF4G3 for mouse samples (1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... and antibodies directed against anti-human mitochondria (1:100, Merck #1273;), CDK2AP1 (rabbit ...
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bioRxiv - Bioengineering 2024Quote: ... anti-human nuclei (HuNu; 1:100; MAB4383, Merck Millipore, MA, USA), anti-Ku80 (1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... Protein standards were obtained from Merck.
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... 2.0 Protein Detection System (Merck Millipore), incubated with Clarity Western ECL (BioRad) ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed in recombinant tau-lysis buffer (1 mM benzamidine, 1 mM PMSF, 1x cOmplete™, EDTA-free Protease Inhibitor Cocktail mix (Merck), 14 mM b-mercaptoethanol ...
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bioRxiv - Biochemistry 2023Quote: In situ protein-protein interaction was evidenced using Duolink® Proximity Ligation Assay (Merck, DUO920082, DUO92004, DUO92008). Cells were fixed with formaldehyde and permeabilized using ice-cold 90% methanol ...
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bioRxiv - Biochemistry 2020Quote: ... 500 μM ATP and ~0.5 μg commercially available human CyclinB-Cdk1 (Merck). The reaction was carried out at 30 °C and was stopped using SDS-PAGE sample buffer after 5 minutes.
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bioRxiv - Cell Biology 2019Quote: ... Human pancreatic organoids were additionally immuno-stained against Sox9 (primary antibody: Merck Millipore ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... a rabbit anti-human EGFR monoclonal antibody (EMD Millipore-MERCK, Temecula, USA) diluted 1:1000 or a rabbit anti-human VEGF-A polyclonal antibody (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... human tau constructs were expressed in the vector pNG2 (Merck-Novagen, Darmstadt) in E ...
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bioRxiv - Immunology 2021Quote: ... MILLIPLEX MAP Human High Sensitivity T Cell Panel (Merck Cat# HSTCMAG-28SK) were used ...
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: ... 501µg/ml Ascorbic acid and 180⍰µg/ml Human transferrin (Merck, T8158)) and then plated onto non-adherent dishes (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... Immortalized human cerebral microvascular endothelial cell line (hCMEC/D3 Merck Life Science) were cultured on 250µg/ml collagen-I (Advanced Biomatrix ...