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Citations for Merck :
151 - 200 of 1379 citations for Recombinant Human S100 Calcium Binding Protein A9 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... Non-specific binding sites were blocked via incubation in 3% H2O2 (Merck, Germany) and BSA respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Unspecific binding sites were blocked using 5% normal goat serum (NGS, S26, Merck) for 1 hour ...
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bioRxiv - Molecular Biology 2019Quote: ... protocol with the administration of recombinant FSH (Gonal-F, Merck Serono ...
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bioRxiv - Biochemistry 2021Quote: ... A complex of recombinant bovine CaM (cat. no. 208690, Merck), with an amino acid sequence identical to the human isoform ...
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bioRxiv - Developmental Biology 2021Quote: ... Patients were injected with recombinant FSH (Gonal-F, Merck, Italy) on day 2 of their menstrual cycle with a starting dose of 150 IU/d ...
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bioRxiv - Cell Biology 2019Quote: ... kidneys were removed and washed in Hanks’ Balanced salt solution (HBSS containing calcium chloride and magnesium sulphate, Merck). Kidneys were then cut into small pieces and further dissociated by incubation for 30 min at 37°C in HBSS with 1 mg/ml collagenase (type 2 ...
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bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation ...
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bioRxiv - Biochemistry 2022Quote: ... and visualized using a combination of anti-mono-ADP-ribose binding reagent (Merck, Germany) and anti-rabbit-HRP antibody (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... and binding was detected by the addition of the ECL chemiluminescent reagent (Merck-Millipore). The light generated was measured using Spectramax M3 plate reader at a wavelength of 700 nm.
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bioRxiv - Molecular Biology 2020Quote: ... DNA-calcium phosphate transfection complex was prepared by gently adding plasmid DNA and 2M CaCl2 (Sigma-Aldrich; Merck KGaA) dropwise to HBS buffer (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: Calcium-free TAP-Ca medium was prepared by dissolving 2.42 g of Tris-Base (93362, Merck KGaA, Darmstadt, Germany) to 850 mL deionized water ...
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bioRxiv - Developmental Biology 2021Quote: ... and 75 mIU/mL of recombinant follicle stimulating hormone (Merck Serono). The oocytes were randomly assigned to experimental groups ...
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bioRxiv - Biochemistry 2022Quote: ... Standard curves were generated using recombinant HIV-1 RT (Merck Millipore). The relative viral quantities were calculated based on the standard curve generated using QuantStudio-7 systems software (Applied Biosystems).
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were supplemented with 500 U Recombinant DNAse (Merck 4716728001) and 100 mM PMSF ...
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bioRxiv - Biochemistry 2022Quote: ... 1000 U/mL recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore) and 50 U/mL Penicillin–Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... ESGRO recombinant mouse Leukaemia Inhibitory Factor (LIF) was obtained from Merck Millipore ...
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bioRxiv - Genomics 2020Quote: ... 1000 U/ml recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore), and 50U/ml Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... recombinant FSH (Gonal-f 75 UI, Merck Serono Europe Limited, Spain), urinary human menopausal gonadotropin (hMG ...
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bioRxiv - Genomics 2022Quote: ... 1000 U/ml recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore), and 50U/ml Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Human HDL (Merck Millipore) was incubated with NS1 for 1 hour at 37°C prior to the DSC experiments ...
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bioRxiv - Immunology 2021Quote: Native human insulin (Merck) was pre-diluted in water to 1 mg/mL ...
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bioRxiv - Bioengineering 2020Quote: Fibrinogen stock solutions were prepared by dissolving 1 g of human fibrinogen lyophilized from 20 mM citrate pH 7.4 solutions (Merck 341576, >90% clottable proteins) into distilled water as recommended by the manufacturer (final volume of app ...
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bioRxiv - Molecular Biology 2022Quote: 200 μg Pol II was hyperphosphorylated by incubation with 280 units of human MAP Kinase 2/Erk2 Protein (Merck, cat. No, 14-550) in a total volume of 50 μl in kinase buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on a 2 ml HIS-SelectTM Nickel Affinity Gel (Merck) and washed with wash buffer ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on a 2 ml HIS-SelectTM Nickel Affinity Gel (Merck) and washed with wash buffer ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore ...
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bioRxiv - Cell Biology 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
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bioRxiv - Biophysics 2023Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosetta 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 µg/ml kanamycin and 34 µg/ml chloramphenicol ...
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bioRxiv - Biophysics 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Molecular Biology 2023Quote: ... or HIS-Select Nickel Affinity Gel in a gravity-flow column (Merck, Darmstadt, Germany), followed by washing with binding buffer with and without 1.3% Triton X-100 ...
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bioRxiv - Cell Biology 2022Quote: ... followed by incubation with 1:500 dilution of anti-pan-ADPr-binding reagent (Merck #MABE1016) in TBST for 1 hr at RT ...
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bioRxiv - Microbiology 2022Quote: ... Half of the harvested supernatant was filtered using fast flow & low binding filters (Merck Millipore).
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bioRxiv - Immunology 2023Quote: ... they were detected for intermolecular binding using Duolink flow PLA detection kit (Green) (Sigma/Merck) with a reaction time of 100 minutes for post-mouse/rabbit probe linking amplification ...
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bioRxiv - Neuroscience 2023Quote: ... Cells from identical conditions were collected and pooled in 10 ul in calcium-free and magnesium-free HBSS (Merck, H8264) + 50% FBS (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: Organoids were washed with DPBS without calcium and magnesium and added to a 9:1 mixture of Accutase (Merck, A6954) and 10x Trypsin (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... DL-dithiothreitol (DTT, #D5545), iodoacetamide (IAA, #I1149), calcium chloride (CaCl2, #A4689), and sodium dodecyl sulfate (SDS, #75746) were purchased from Merck. Sequencing grade ...
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bioRxiv - Cell Biology 2020Quote: ... the GFP-Trap bead bound fraction was incubated with recombinant TEV (Merck) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were treated with recombinant mouse TNFα (10 ng/ml, Merck 654245).
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bioRxiv - Microbiology 2021Quote: 10His-tagged MA gene was expressed in Escherichia coli (E. coli) strain BL21 (DE3) (Merck, Darmstadt, Germany). The 10His-tagged MA protein was initially purified with nickel-NTA affinity column chromatography followed by TEV cleavage ...
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bioRxiv - Cell Biology 2019Quote: Human fibronectin was from Merck Millipore ...
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bioRxiv - Developmental Biology 2021Quote: ... 5.06% fibrinogen (human plasma; Merck), 3U/mL thrombin (Stago BNL ...
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bioRxiv - Microbiology 2022Quote: ... Plasma-purified human thrombin (Merck) was then added to reactions as required from a 1 IU / μL stock ...
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bioRxiv - Developmental Biology 2022Quote: ... The beads were then washed five times with binding buffer with 0.05% IGEPAL CA630 (Sigma, Merck). One tenth of the beads were eluted in loading buffer for Western blot purposes and the rest was processed for Mass Spectrometry analysis.
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bioRxiv - Genetics 2021Quote: Before the ovaries were stimulated with recombinant FSH (Gonal-F, Merck Serono, Switzerland), downregulation was performed using a GnRH agonist (Decapeptyl ...
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bioRxiv - Zoology 2020Quote: ... Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck) in TE buffer (10 mM Tris-HCI ...
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bioRxiv - Cell Biology 2022Quote: All experiments were performed using the BDNF mature recombinant (GF029; Merck Millipore, USA) or the C-ter truncated form of BDNF (BDNFΔC-ter) ...
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bioRxiv - Plant Biology 2022Quote: ... The filtered supernatant was loaded onto a column packed with cOmpleteTM His-tag purification resin (Merck). His6-proteins were eluted with 4x 2 bead volumes of elution buffer (20 mM Na HEPES ...
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bioRxiv - Evolutionary Biology 2020Quote: ... F2 FLAG-tagged TBP::NveRLR::mCherry and wild-type zygotes were treated with 3% L-Cysteine (Merck Millipore), washed and snap frozen in liquid nitrogen ...
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bioRxiv - Immunology 2022Quote: ... Human heat-aggregated IgG immune complexes (ICs) were prepared by heating human IgG1 isotype control (Merck) for 20 min at 63°C.