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Citations for Merck :
301 - 350 of 446 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... CM was separated into three fractions by three centrifugal filter devices (3 kDa, 10kDa and 30 kDa) (Merck Millipore). 10% CM ...
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bioRxiv - Developmental Biology 2021Quote: ... Equal amounts of protein (30-40 μg/sample) were resolved on a 10% polyacrylamide gel and transferred onto a PVDF membrane (Immobilon-E, Merck). The membrane was blocked with 5% milk in TBS containing 0.1% Tween20 (TBS-T ...
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bioRxiv - Microbiology 2020Quote: ... After 2 hours at 30°C with shaking 1 ml of cells was pelleted and soluble proteins were extracted using BugBuster (Merck). For internalisation experiments involving plasmid-complemented deletion strains ...
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bioRxiv - Molecular Biology 2021Quote: ... The sample (0.5-1 ml) was transferred to Amicon Ultra 0.5 ml Centrifugal Filters (MWCO=30 kDa, Merck, cat# UFC503096) and further concentrated to a final volume of 200 μl.
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bioRxiv - Neuroscience 2021Quote: ... 0.1 M Tris pH 8.5) and processed according to a CHAPS-based FASP protocol in centrifugal filter units (30 kDa MWCO, Merck Millipore). After removal of the detergents ...
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bioRxiv - Physiology 2020Quote: ... Fusion proteins were exchanged into low (< 30 mM) imidazole buffer using an Amicon Ultra-15 centrifugal concentrators (Merck Millipore, Germany) with a 30 kDa molecular weight cut-off in preparation for liberation of Hi1a from the fusion tag using tobacco etch virus (TEV ...
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bioRxiv - Biochemistry 2020Quote: Protein pellets were dissolved in 50 μl of 8 M Urea and loaded onto a 30 K centrifugal filter unit (Merck). Preparation for MS was done as described in [64] ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked with 10% bovine serum albumin (BSA) 30 min at 37°C and incubated with primary anti-γH2A.X mouse antibodies (Merck Millipore) or rabbit anti-LC3B antibodies (Cell Signaling Technology ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was neutralised immediately using 1.5 M Tris pH 8.6 and then buffer exchanged to PBS using a 15 ml 30 kDa MWCO centrifugal filter (Merck Millipore).
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bioRxiv - Neuroscience 2022Quote: ... 0.1 M Tris pH 8.5) and processed according to a CHAPS-based FASP protocol in centrifugal filter units (30 kDa MWCO, Merck Millipore). After removal of the detergents ...
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bioRxiv - Microbiology 2022Quote: ... and DNA was eluted in 30 µL of autoclaved ultrapure water (Milli-Q Reference Water Purification System, Merck & Co., USA). DNA was quantified with a Qubit 2.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The purified proteins were concentrated to 2 mg/mL using centrifugal filters with either 3 kDa or 30 kDa cut-off (Amicon Ultra-0.5, Merck/Millipore) and the samples were centrifuged at 100,000 g ...
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bioRxiv - Immunology 2022Quote: ... supernatants were harvested by centrifugation (30 min at 4000 rpm) and filtration using a 0.22 µm Steritop filter (Merck Millipore). Supernatants containing mAbs were purified as described previously (Brouwer et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... permeabilized for 30 minutes at room temperature in PBS supplemented with 0.2% Triton X-100 and 1% Bovine Serum Albumin (BSA) and blocked for 30 minutes at room temperature in 10% donkey serum (all from Merck). Samples were stained overnight at 4°C with the primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... alkylated with 40 mM Chloroacetamide (CAA) at RT for 30 min and protein amount was quantified using the Direct Detect Spectrometer from Merck. Protein digestion was performed using the Single-Pot Solid-Phase-enhanced Sample Preparation approach SP3 ...
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bioRxiv - Molecular Biology 2022Quote: ... Peak fractions were pooled and concentrated using a 30 kDa cut-off Amicon Ultra-15 concentrator (Merck, cat No. UFC903024) to a typical concentration of about 6 mg/ml ...
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bioRxiv - Immunology 2022Quote: ... 500ng/ml purified total IgG stimulation was added the next day for 4 hrs followed by an incubation of 30 min with ATP (Merck). Culture supernatants were collected for further cytokine analysis and cells lysates were prepared for qPCR analysis ...
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bioRxiv - Cell Biology 2022Quote: ... Bacteria and debris were then pelleted by centrifugation at 5,200 x g for 30 min and the supernatants filtered using a vacuum-driven Stericup filtration system at 0.22 μm (Merck Millipore).
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bioRxiv - Evolutionary Biology 2022Quote: ... the final ribosome solution was diluted with 70S buffer 10-fold and then concentrated using Amicon Ultra (30 kDa cut, Merck) three times.
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bioRxiv - Molecular Biology 2022Quote: Cells were centrifuged at 3041 × g for 30 minutes at 4°C and supernatant was applied to a 500 mL Stericup-HV sterile vacuum filtration system (Merck) with a pore size of 0.22 µm ...
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bioRxiv - Cancer Biology 2022Quote: ... were sonicated in 1 M KOH solution (6592-3705, Daejung) for 30 min and then washed with Milli-Q water (Direct 8, Merck) to remove remaining KOH solution ...
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bioRxiv - Neuroscience 2023Quote: ... Protein extracts were processed by following the filter-aided sample preparation (FASP) protocol with the Microcon 30-kDa centrifugal filter device (MRCF0R030, Merck) for detergent removal ...
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bioRxiv - Immunology 2024Quote: ... The ethanol and unloaded nucleic acids in the mixtures were removed using Amicon Ultra centrifugal filters (10- or 30-kD MWCO, Merck) for two rounds with 5- and 15-times volumes of 1× PBS ...
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bioRxiv - Immunology 2024Quote: ... The lentivirus particles were centrifuged onto the cells for 90min at 500g at 30°C in the presence of 4μg/ml polybrene (Sigma-Aldrich/Merck). Afterwards ...
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bioRxiv - Cancer Biology 2024Quote: ... The lyophilized peptides were resolubilized in 80 % acetone with 1 % TFA and loaded onto an ihouse ZIC-HILIC micro-column containing 30 mg of ZIC-HILIC particles (Merck Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated in T cell medium for 30 min at 37°C without any metabolic inhibitors or in presence of 1 μM oligomycin (Merck), 100 mM 2DG (Merck) ...
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bioRxiv - Microbiology 2023Quote: Faeces samples for 16S-rRNA gene amplicon sequencing and metaproteomics were collected in 30-mL stool containers (Greiner 443102, Merck) at baseline ...
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bioRxiv - Molecular Biology 2023Quote: ... Switzerland) and further processed by tangential flow filtration (30 kDa MWCO, PES membrane; Pellicon XL 50; Millipore, Merck, Darmstadt, Germany), autoclaved ...
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bioRxiv - Biochemistry 2023Quote: ... The next day the proteins were collected and concentrated using 30 kDa molecular weight cut-off centrifugal filters (Merck Millipore) for size exclusion chromatography.
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bioRxiv - Biochemistry 2023Quote: ... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
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bioRxiv - Microbiology 2023Quote: ... LB plates were seeded with the different cultures and Whatman n° 1 filter discs (6 mm) were impregnated with 5 μl of 30% (v/v) H2O2 (Merck) as previously described [31] ...
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bioRxiv - Microbiology 2023Quote: ... the solution was transferred to a Millipore Amicon Ultra 30 kDa cut off membrane centrifugal filter unit (Merck Millipore, USA) (prewashed twice to remove glycerol ...
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bioRxiv - Plant Biology 2023Quote: ... alkylation and tryptic digestion (50 µg of protein per sample) was performed in centrifugal filters (Amicon Ultra-0.5, 30 kDa cut-off, Merck Millipore) according to the FASP protocol (Wiśniewski et al. ...
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bioRxiv - Genetics 2023Quote: ... neo-synthesized DNA was labeled by two successive pulses (30 min each) with IdU 20 μM (5-iodo-2’-deoxyuridine, 57830, MERCK) and CldU 100 μM (5-chloro-2’-deoxyuridine ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 4% SDS and 0.1 M DTE) and buffer exchange was performed in Amicon Ultra Centrifugal filter devices (0.5 mL, 30 kDa MWCO; Merck Millipore) at 14,000 rcf for 15 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei were recovered by withdrawing a volume of 200 μL from the 30 – 40% interface of the OptiPrep density gradient and transferring to a new 2 mL Sorenson Dolphin microcentrifuge tube (Merck). Nuclei were washed with 2% BSA (in 1x PBS ...
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bioRxiv - Microbiology 2024Quote: ... the coverslips were fixed in 1% glutaraldehyde in 1x PBS for 30 min and stained with filtered 10% Giemsa (Merck) in 1x PBS for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... complemented with 10 % (v/v) heat-inactivated (56°C at 30 min) FBS and 200 µM BAPTA (Merck Millipore, 196418)] with or without 1.8 mM CaCl2 (Roth ...
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bioRxiv - Neuroscience 2024Quote: ... the brains were removed and post-fixed for 1–2 days before transfer to 30% sucrose (Merck KGaA, Darmstadt, Germany) dissolved in PBS ...
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bioRxiv - Plant Biology 2024Quote: ... a second extraction was performed by adding 30 mL of extraction solution consisting of 95% (w/v) methanol (Merck Uvasol), 4.95% (w/v ...
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bioRxiv - Immunology 2024Quote: ... After incubation at room temperature for 30 minutes, the PBMCs were washed with PEB buffer (PBS, pH 7.2, 0.5% bovine serum albumin (#SLCF3210 Merck-Sigma, USA) and 2 mM EDTA (Merck-Sigma ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was then concentrated with a 0.5 mL 30 kDa MW cut-off centrifugal filter (Amicon UItra, Merck Millipore) and purified by SEC using octamer buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The cultures were incubated for 5 days at 30 °C and 130 rpm (Multritron, INFORS) using AeraSeal films (Merck, #A9224) on top of the flask ...
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bioRxiv - Cell Biology 2024Quote: ... followed by two rounds of selection alternating between BCDA medium containing the appropriate antibiotic (30 µg/mL hygromycin B [Carl Roth], 20 µg/mL G418 [Merck] ...
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bioRxiv - Biophysics 2021Quote: ... Freshly excited slices were incubated for 30 min at 37°C in a solution of Nile Red (Cat. No. 72485-100MG, Sigma-Aldrich/Merck) pre-dissolved in methanol at a concentration of 1 mg/ml and diluted 1:20 in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... All supernatant was removed using a 1 ml syringe and the beads were resuspended in 30 μl 2X SDS Sample Buffer (Merck Millipore). The samples were incubated at 98°C for 5 minutes before electrophoresis.
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bioRxiv - Genomics 2022Quote: ... in 3×250 ml bottles and resuspended in 100 ml prewarmed 30°C YPD supplemented with 50 ug/ml Pronase (Merck 10165921001), at which point the count-up for the time course was initiated ...
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bioRxiv - Biophysics 2020Quote: ... DNA origami were incubated with a 10x molar excess of dSA for 30 min at 24°C and excessive dSA was removed using 100kDa Amicon®Ultra centrifugal filters (UFC210024, Merck). As a last step ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were removed and post-fixed for 1-2 days and then transferred to 30% sucrose (Merck KGaA, Darmstadt, Germany) dissolved in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... MAP2 and/or PSD-95 were immunostained using primary antibodies diluted in PBS-BSA 1% for 30 min (SP: rabbit polyclonal, Synaptic System, 163 002, 1:600; VGLUT1: guinea pig polyclonal, Merck Millipore ...