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Citations for Merck :
201 - 250 of 446 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... in 30% (2-Hydroxypropyl)-β-cyclodextrin (HPβCD) or Sulfobutylether-β-Cyclodextrin (SBEβCD) (Sigma Aldrich, Merck, Germany). When tumors reached a size of 60-100 mm3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... the proteins were concentrated to 15 – 30 mg/ml concentration using Amicon Ultra Centrifugal filters (Merck) via short (2 – 5 min ...
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bioRxiv - Immunology 2023Quote: ... The tissue was stained for 30 min in ORO solution (0.5% in isopropanol, Sigma Aldrich/Merck) at RT ...
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bioRxiv - Microbiology 2023Quote: ... solution for 30 min at RT and washed for 20min in a 10% formamide (Merck, #F9037), 2X SSC (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... were prepared as free-floating sections in anti-freeze solution (30% sucrose (Merck Life Science, 84100); 30% glycerol (VWR ...
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bioRxiv - Genetics 2024Quote: ... Membranes were blocked for a minimum of 30 min in 5% Skim Milk Powder (Merck, #70166) in PBS + 0.1% Tween-20 (PBS-T ...
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bioRxiv - Physiology 2024Quote: ... followed by 1% acetic acid for 30 seconds and Safranin’O solution for 45 min (S2255, Merck).
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bioRxiv - Cell Biology 2024Quote: ... samples were washed again for approximately 30 min and incubated with DAPI (Merck, Cat. Nr. D9542) for 10 min ...
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bioRxiv - Plant Biology 2020Quote: ... 0.1 g of the ground material was digested overnight at 90°C in a mixture of 2 ml HNO3 and 0.5 ml H2O2 (30% Suprapur, 1.07298.1000 Merck). Then 50 μl of HF (40% reagent grade ...
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bioRxiv - Immunology 2022Quote: ... The eluted fractions were cleaned and concentrated in Amicon Ultra-2 30 kDa (Merck Millipore, cat: UFC203024) by diluting them in PBS (150 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... 3 mM MgCl2) using Amicon Ultra-0.5 mL Centrifugal Filters (30 or 100 K MWCO, Merck Millipore). After re-measuring the concentrations by densitometry ...
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bioRxiv - Microbiology 2020Quote: ... dehydrated in gradual ethanol (30–100%) and propylene oxide followed by embedding in Epon (Merck, Darmstadt, Germany). Semithin sections were stained with toluidine blue ...
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bioRxiv - Microbiology 2021Quote: ... GGG-azide labelled proteins were concentrated to 25 μM on a 30 kDa Amicon Tube (Merck Millipore) in Tris/NaCl buffer and next labelled with 100 μM DBCO-Cy5 (Sigma Aldrich ...
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bioRxiv - Systems Biology 2022Quote: ... samples were thawed and ultrafiltered using a 30 kDa filter unit (Amicon-30kDa; Merck Millipore, Darmstadt, Germany) for 10 min at 13000g and 4°C in order to remove macromolecules and active enzymes ...
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bioRxiv - Neuroscience 2022Quote: ... cryoprotected several days in a solution of PBS 0.01 mM/sucrose 30% (w/v, 1076511000, Merck Millipore), and then cut into 50 μm slices with a sliding microtome (SM2010 R ...
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bioRxiv - Immunology 2022Quote: ... centrifuged at 4000 rpm for 30 min and filtered using 0.22 μM Steritop filter units (Merck Millipore). Affinity chromatography with Ni-NTA agarose beads (Qiagen ...
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bioRxiv - Plant Biology 2024Quote: ... and proteins were concentrated using a 30 kDa Amicon Ultra-4 Centrifugal Filter Unit (Merck Milipore; www.merckmillipore.com). Protein concentration was determined employing the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... 20 μg of total protein were transferred onto centrifugal filter units (Microcon-30 kDa; Merck, Darmstadt, Germany) and washed with a buffer containing 8 M urea and 100 mM Tris (pH 8.5) ...
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bioRxiv - Biophysics 2023Quote: ... The TEV-cleaved protein was then buffer exchanged using a 30 kDa MWCO centrifugal filter (Amicon, Merck) into buffer containing no imidazole and 50 mM KCl (25 mM K·HEPES ...
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bioRxiv - Microbiology 2023Quote: ... diluted 1 : 100 in 1 × PBS buffer (pH 7.5) with 0.1 % hydrogen peroxide (stock concentration 30 %, Merck) and 2 mM ATP (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions were concentrated on AmiconUltra centrifugal filters (molecular weight cut-off, MWCO of 30 kDa, Merck Millipore) by centrifuging at 7,500 g and pulled for dialysis into buffer containing 50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: ... Two-week-old seedlings (three leafs) were exposed to 30% PEG in distilled water (PEG 6000; Merck) for 18-20 h ...
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bioRxiv - Biophysics 2023Quote: ... the purified tubulin was concentrated and desalted on an Amicon Ultracel-30 K filter (Milipore, Merck KGaA) with BRB80 and 0.1 mM GTP ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated overnight with 30 μL ChIP-grade Protein G agarose beads (Merck Millipore, Germany) and 10 µg DNMT1 ...
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bioRxiv - Microbiology 2024Quote: ... The flowthrough was collected and concentrated using 30 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 200 (16/600 ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted and precleared with 30 μL protein A/G agarose beads (Merck-Millipore, 16-157/16-201) for 1h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were concentrated using Vivaspin 20ml 10 kDa or 30 KDa MWCO concentrators (Merck Z614602-12EA) and stored in aliquots at -70 °C.
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bioRxiv - Physiology 2024Quote: ... Centrifugal Filter Units Amicon® Ultra-4® Centrifugal Filters Ultracell 30 K (Merck Millipore, Molsheim, France). Polypropylene high clarity polyethylene (ISO 12485/ISO9001 ...
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bioRxiv - Cell Biology 2024Quote: ... containing the λ PPase protein was concentrated using a 30 kDa cut-off Amicon filter (Merck Millipore) and loaded onto a pre-equilibrated Superdex 200 Increase 10/300 GL column (Cytiva) ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 mM ethylenediaminetetraacetic acid (EDTA)) using an ultra-centrifugal filter (Amicon ultra-4 [30 kDa]; Merck). The (His)6-tag was cleaved by reacting with HRV3C overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Derivatisation was achieved by the addition of 25 µL methoxyamine hydrochloride (30 mg/mL in pyridine, Merck) followed by shaking at 37°C for 2h ...
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bioRxiv - Biochemistry 2020Quote: ... and relevant fractions were pooled and concentrated using a 30 kDa molecular weight cut-off centrifugal concentrator (Merck). The his-tag was removed by incubating concentrated protein overnight with Tobacco Etch Virus (TEV ...
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bioRxiv - Developmental Biology 2021Quote: ... 20% and 30% sucrose (w/v) in PBS before embedding in Optimum Cutting Temperature (OCT) medium (Merck, F4680). 12 μm thick frozen sections were collected and permeabilised using 0.3% Triton-X in PBS for 15 min ...
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bioRxiv - Biophysics 2021Quote: ... for 2 hours at 37°C and purified using an Amicon 30 kDa MWCO filter (Merck, Darmstadt, Germany). The Deep Vent exo- DNA polymerase (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... After 24 h cells were stained for 30 min at 37 °C with NAO dye (200 nM, Merck), washed ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EGTA and protease inhibitors) on an Amicon 30 kDa cut off concentrator (Merck, Cat# UFC 503096). Protein concentration was determined using a BCA protein assay (Thermo Scientific ...
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bioRxiv - Biophysics 2022Quote: ... for 2 hours at 37°C and purified using an Amicon 30 kDa MWCO filter (Merck, Darmstadt, Germany). Deep Vent exo-DNA polymerase (New England Biolabs ...
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bioRxiv - Biophysics 2022Quote: ... using an Amicon® Ultra (molecular weight cut-off 30 kDa) centrifugal filter device (Merck Millipore, Darmstadt, Germany). Samples were kept frozen at -80 °C for future use.
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bioRxiv - Cell Biology 2022Quote: ... coli cells using 0.2 mM IPTG for 4 hours at 30 °C and purified on flag beads (Merck) and eluted using flag peptide buffer (200 µg/ml flag peptide in 10 mM Tris-HCL ...
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bioRxiv - Immunology 2020Quote: ... Protein was concentrated to 1 ml using a Amicon Ultra-4 Ultracel 30 kDa spin concentrator (Merck Millipore) and the protein was separated on a HiPrep 16/60 Sephacryl S-200 HR column (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde for 30 min and stained with 1% crystal violet (Merck, Darmstadt, Germany) for 20 min at RT ...
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Adipose Triglyceride Lipase protects the endocytosis of renal cells on a high fat diet in DrosophilabioRxiv - Cell Biology 2020Quote: ... pericardial nephrocytes were incubated for 30 min at 25°C in Schneider’s Medium with 0.5 mg/ml delipidated BSA (A9205, Merck) and 10 μM BODIPY FL C12 green fluorescent fatty acid ...
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bioRxiv - Molecular Biology 2020Quote: ... and plates were developed in n-hexane/diethylether/acetic acid 70:30:5 (v/v/v) (Merck, Roth) in a developing chamber (CAMAG ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 8.5 and centrifuged at 14,000 g for 20 minutes using a 30 kDa mass-cutoff 0.5 ml Amicon centrifugal filter (Merck). For alkylation of cysteines ...
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bioRxiv - Biochemistry 2021Quote: ... the VmeCmr complex-containing flow-through was concentrated using an Amicon Ultracentrifugal filter (30 kDa MWCO, Merck-Millipore) and further purified by size exclusion chromatography (HiPrep™ 16/60 Sephacryl® pg 300 HR ...
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bioRxiv - Cell Biology 2021Quote: ... ligation for 30 min 37 °C and polymerase reaction overnight at 37 °C according to manufacturer’s protocol (Merck, Duolink® In Situ Detection Reagents Red ...
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bioRxiv - Immunology 2021Quote: ... inserts and cut vector were dialysed for 30 minutes using 0.025 µm MF-Millipore membrane filters (Merck VSWP01300) and purity of final products was confirmed by agarose gels and concentration measured using NanoDrop 2000c (Thermo Scientific ND-2000) ...
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bioRxiv - Cell Biology 2022Quote: ... then brought to 10 ml with ddH2O) and ECL2 solution (6 µl 30% (v/v) H2O2 (Merck, #H1009) in 10 ml with ddH2O ...
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bioRxiv - Molecular Biology 2022Quote: ... The Swi2C-Swi5 complex was concentrated using an Amicon Ultra-4 (30 k) centrifugal filter (UFC803008, Merck Millipore). After cleavage ...
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bioRxiv - Cancer Biology 2024Quote: ... T865.3) was followed by 5 minutes of rinsing with water and 20-30 seconds of Eosin solution (Merck, 115935 ...