Labshake search
Citations for Merck :
301 - 350 of 2383 citations for Nuclear pore complex protein Nup93 dye Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... biofilm samples were rinsed with deionized water and deposited on polycarbonate filters (GTTP Isopore membrane filters, Merck Millipore, pore size 0.2 μm) or on glass slides (for correlative Raman analyses ...
-
bioRxiv - Microbiology 2021Quote: ... groundwater was pumped directly into a sterile chilled Nalgene filtration unit fitted with a 0.22 µm pore size Isopore polycarbonate membrane (Merck Millipore, Darmstadt, Germany). Approximately 10 L of groundwater was filtered each for metagenomic and metaproteomic sample and 1 L was filtered when samples were taken for 16S rRNA gene amplicon analysis only ...
-
bioRxiv - Microbiology 2021Quote: ... approximately 50 - 100 L of groundwater was filtered sequentially through 0.2 µm and 0.1 µm pore sized PTFE filters (142 mm, Omnipore Membrane, Merck Millipore, Germany; Table S1). With the exception of H32 (did not yield sufficient volumes) ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were seeded on the upper chamber of 24 well plate cell culture inserts containing 3 μm pores (Merck, Cat # 353096) coated with rat-tail collagen I (Sigma ...
-
bioRxiv - Microbiology 2022Quote: ... 0.1 mL of fixed cell suspension was filtrated by 0.22-µm pore-sized black polycarbonate filter (type GTBP; Merck Millipore, Darmstadt, Germany) and stained with 10 × SYBR Green I (SYBR® Green I Nucleic Acid Stain ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cell viability was assessed by trypan blue exclusion and 3 × 106 viable cells from each condition were loaded into the upper chambers of Transwell culture inserts with a 0.5 µm pore size (Merck, Burlington, MA, USA). Cells were allowed to migrate towards the lower well containing medium ...
-
bioRxiv - Microbiology 2021Quote: ... the supernatant was harvested by centrifugation at 10,000 x g at 4°C for 30 min and filter-sterilized using a 0.45 μm pore size hydrophilic PVDF membrane (Millex®-HV 0.45 μm, Merck Millipore, Darmstadt, Germany).
-
bioRxiv - Biochemistry 2021Quote: ... free radioligands bound to DCC were removed using a vacuum filtration system with a 96-well filtration plate (MultiScreenHTS HV, 0.45-mm pore size, Merck KGaA, Darmstadt, Germany) for the bound/free (B/F ...
-
bioRxiv - Cell Biology 2021Quote: ... and transferred to Immobilon 0.20 μm (LC3, GAPDH) or 0.45 μm (CD9, CD63) pore-sized PVDF membranes (Merck Millipore Ltd., Cork, Ireland) by wet transfer ...
-
bioRxiv - Cell Biology 2021Quote: ... Retinal pieces were cultured on a cell culture insert with a 0.4 µm pore diameter (Millicell PICM0RG50, Merck Millipore Ltd, Cork, Ireland) in DMEM/Ham’s F-12 without Phenol Red (Nacalai Tesque ...
-
bioRxiv - Microbiology 2024Quote: ... for 10 minutes in the dark and then filtered through Nuclepore black polycarbonate membrane filters (0.22 µm pore size, Merck Millipore, Darmstadt, Germany) with negative pressure (≤ 20 kPa) ...
-
bioRxiv - Microbiology 2022Quote: ... Dust samples were collected by impaction on preconditioned and pre-weighted 25 mm hydrophilic polycarbonate membrane filters with a pore size of 0.8 µm (Merck KGaA, Darmstadt, Germany) mounted in antistatic polypropylene total dust filter cassettes (Pall Laboratories ...
-
bioRxiv - Microbiology 2022Quote: ... 0.5 ml of each aliquoted sample was filtered onto a 25 mm polycarbonate filter (pore size: 0.45 µm, Merck KGaA, Darmstadt, Germany). The filters were then air dried under sterile conditions and mounted onto 25mm diameter aluminum pin stubs (Agar Scientific Ltd. ...
-
bioRxiv - Biochemistry 2022Quote: ... Culture supernatants were harvested 24 h post-viral infection and then passed through 0.22 μM pore-sized filters (Merck Millipore, Carrigtwohill, Ireland) to remove cell and debris and obtain viral suspension for experiments.
-
bioRxiv - Microbiology 2024Quote: ... The conidial samples were then suspended by 2 mL of sterile water and filtered through Miracloth cloths (pore size 22-25 μm, Merck KgaA, Germany) to remove mycelial fragments ...
-
bioRxiv - Microbiology 2024Quote: We plated 50μL of each co-culture mixture in triplicate on sterile 13mm Mixed Cellulose Ester (MCE) filter papers (pore size 0.22μm, Merck Life Science UK Ltd.), placed flat on pre-dried ...
-
bioRxiv - Microbiology 2024Quote: ... The micelle was resuspended in Milli Q water was subjected to an additional filtration process using a sterile syringe filter (0.22 μm pore size, Millex®, Merck, Mumbai, India) to eliminate remaining free Cip ...
-
bioRxiv - Microbiology 2024Quote: ... pH 7.5) and the capsid solutions were filtered once (centrifugal filter unit Ultrafree MC, pore size of 100 nm, Merck KGaA, Darmstadt, Germany). The concentrations of the P1 ...
-
bioRxiv - Bioengineering 2024Quote: ... The CD45 positive and negative samples were resuspended in 500 μl DPBS and filtered through 70 µm pore sterile Flowmi Cell Strainers (Merck, Sigma Aldrich). Acridine orange and propidium iodide (Allied Genetics Inc ...
-
bioRxiv - Molecular Biology 2023Quote: ... 20 15 cm dishes were seeded and the nuclear translocation of SAMD1 was induced 24 h before extract preparation by adding 200 nM 4-OHT (Merck; 68392-35-8). After collection ...
-
bioRxiv - Developmental Biology 2021Quote: ... Proteins were detected by incubation with primary antibodies (SOX2, Bio-techne, AF2018, 1: 1000 and α-Tubulin, Merck, T6199, 1:2000) and secondary antibodies (Donkey anti-Goat IRDye® 800CW ...
-
bioRxiv - Cell Biology 2024Quote: ... of cell lysates by incubating lysates with 0.5-2.5 µg of antibodies and 20 µl of protein G–Sepharose (Merck, GE17-0618-01) at 4°C for 2 h ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were permeabilized with 100% ice-cold methanol for 20 min at -20°C and rehydrated by washing with PBS and then stained for viral hexon protein expression with an anti-hexon primary antibody (mouse; Merck; #MAB8052) and an anti-mouse secondary antibody coupled to AF488 or AF647 (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins were resolved on a 10% SDS-PAGE gel and Western blotting was performed using an anti-myc antibody (Merck, UK). Co-immunoprecipitation of Ssk2-HA was detected using an anti-HA antibody (Merck ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 300 µg of protein was then incubated with 1 µg of a mouse anti-53BP1 antibody (1μg/ml, Merck millipore, MAB3802) O/N at 4 °C ...
-
bioRxiv - Biochemistry 2020Quote: ... followed by the carbon source (glucose crystals or 99 % glycerol) and filtered sterile using a 0.22 μm pore-size Millex®GP PES filter (Merck-Millipore, Carrigtwohill, Ireland). For the cycA tests ...
-
bioRxiv - Microbiology 2022Quote: ... were sequentially filtered by vacuum through hydrophilic polycarbonate filters with a pore size of 20 µm (47 mm; Sterlitech, Kent, WA, US) and then 2 µm (Isopore, 47 mm; Merck Millipore, Cork, Ireland). Filters were immediately plunged into liquid nitrogen and stored at −80°C until further processing ...
-
bioRxiv - Microbiology 2020Quote: ... One hundred mL of sewage samples were filtered with a 0.22-μm-pore-size mixed cellulose ester membrane (Merck Millipore, Billerica, MA, USA). DNA was extracted from the filtered membranes and from 150–200mg of the nonhuman fecal samples using the ZR Fecal DNA MiniPrep™ Kit (Zymo Research ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were dissolved in 50 μL of Milli-Q water and filtered (0.20 μm pore size, 10 mm diameter, Merck Millipore, Burlington, MA, US)71.
-
bioRxiv - Cancer Biology 2022Quote: ... The remaining peptides were loaded onto primed custom-made HILIC SPE micro-columns packed with ZIC-HILIC resin (10 μm particle size, 200 Å pore size, kindly provided by Sequant/Merck, Umea, Sweden) onto supporting C8 disks (Empore ...
-
bioRxiv - Synthetic Biology 2023Quote: ... by centrifuging the purified enzyme preparations (4,000×g, 4°C) using AmiconTM Ultra-15 centrifugal filters of 10 kDa-pore size (Merck-MilliPoreSigma, Burlington, MA, USA). The absorbance at 280 nm (A280 ...
-
bioRxiv - Neuroscience 2023Quote: ... were cut on a McIlwain tissue chopper and transferred onto 30 mm diameter Millicell culture inserts with 0.4 mm pores (Merck, Fontenay sous Bois, France). Slices were maintained in culture in six-well-plates containing 1 mL of nutrient medium per well ...
-
bioRxiv - Molecular Biology 2023Quote: ... Single-cell suspensions of chondrogenic LMP cells were obtained by filtering through a 20-μm pore-size plastic filter (Millipore, Merck, Billerica, MA, USA). After centrifugation at 800 × g for 10 min at room temperature ...
-
bioRxiv - Cancer Biology 2024Quote: ... The virus was harvested 48 hrs after transfection and filtered through non-pyrogenic filters with a pore size of 0.45 mm (Merck Millipore, Billerica, MA, USA). For infection ...
-
bioRxiv - Neuroscience 2020Quote: ROS levels of cells were detected using the fluorescent dye dihydroethidium (DHE) (Sigma-Aldrich; Merck KGaA). Cells pretreated with TC-HT or HT at 42.5°C high temperature setting for 8 cycles or 2 h ...
-
bioRxiv - Microbiology 2022Quote: ... aeruginosa was measured using the fluorescent dye propidium iodide (PI) as per manufacturer’s instructions (Merck, UK). Late-exponential phase cultures of P ...
-
bioRxiv - Microbiology 2021Quote: ... A dye mixture containing 1.25 μl of a 20 mM CuSO4 solution (99,99% purity, Merck KGaA), 1.25 μl of a 100 mM Tris(3-hydroxypropyltriazolylmethyl)amine solution (THPTA ...
-
bioRxiv - Molecular Biology 2022Quote: ... The following drugs/dyes were used for this work: IFNγ (final concentration of 50ng/mL, Merck), dTAG13 (final concentration of 100nM ...
-
bioRxiv - Immunology 2024Quote: ... slides were rinsed in double-distilled water and counterstained with Hoechst dye (5 μg/mL; Merck). Stained slides were subsequently imaged using an LSM700 confocal microscope (Zeiss).
-
bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissue homogenisation and western blotting was performed with 50 μg of total protein as previously described.15 Membranes were incubated overnight with gentle agitation at 4 °C with the MSH3 antibody (Merck MSH3MAB(1F6)) in 5% milk PSB-T (phosphate buffer saline (PBS) ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 μg/ml ...
-
bioRxiv - Immunology 2020Quote: ... Thirty µl of Protein G (Protein G Sepharose Fast Flow, Merck, UK) slurry per sample was added in addition to 0.01% sodium azide and incubated on a roller overnight at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... Total protein concentration was quantified by means of BCA protein assay (Merck). 10-20 μg of protein was separated by size using a 4-12% Bis-Tris Plus gel ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 µg/ml.
-
bioRxiv - Cell Biology 2024Quote: ... Protein content was determined by performing a BCA protein assay (Merck Millipore). SDS-PAGE was performed on a Mini-PROTEAN System (BioRad ...
-
bioRxiv - Microbiology 2020Quote: ... spores were collected from 5-day-old cultures NO3 medium (0.17% yeast nitrogen base, 3% sucrose, 100mM KNO3) by filtering through miracloth (Merck; pore size of 22–25μm). Spores were centrifuged ...
-
bioRxiv - Developmental Biology 2020Quote: ... The slides were then washed in distilled water and subjected to the filtered Giemsa dye solution (Merck) diluted 1:20 in distilled water for 20 min at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: Intracellular Ca2+ levels were assessed in hippocampal neurons loaded with Fura-2/AM dye (Merck, Darmstadt, Germany) as previously described (Joshi ...