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Citations for Merck :
201 - 250 of 2383 citations for Nuclear pore complex protein Nup93 dye Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... DNA-free containers and subsequent filtration onto 0.1 µm pore size filters of 142 mm diameter (Merck Millipore, JVWP14225) and storage on dry ice / -80 °C until DNA extraction ...
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bioRxiv - Neuroscience 2020Quote: ... Retinas were dissected free in cold HBSS and flat mounted on cell culture inserts (Millicell 0.4 μm pore; Merck) ganglion cell layer up ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Water samples (500 mL) from each bottle were filtered and collected using a Sterivex filter (0.45 µm pore size; Merck Millipore ...
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bioRxiv - Microbiology 2020Quote: ... 70 L of groundwater were filtered onto a 0.1 μm pore-size PTFE membrane filter (Merck Millipore, Darmstadt, Germany). The flow-through was collected in a sterilized container ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-protein samples were separated by electrophoresis using a handmade SDS-polyacrylamide gel (7.5 or 9% polyacrylamide) and transferred to a PVDF membrane (Immobilon-P, pore size 0.45 µm; Merck-Millipore). The membrane was probed with primary antibodies listed in Supplemental Table 3.
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bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered with 5.0-μm and 0.45-μm PVDF pore membrane filters (Millex-HP Syringe Filter; Merck Millipore) to remove bacterial cells ...
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bioRxiv - Molecular Biology 2022Quote: ... by electrophoresis and subsequently transferred onto Immobilon-P™ PVDF membranes of 0.45 μm pore size (Millipore®; Merck). Details of primary antibodies used are provided in Supplementary Table 2 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein transfer was done using the Bio-Rad wet-blotting system at 500 mA for 1 h on 100% methanol-activated low-fluorescent Immobilon-FL membranes (0.45 μm pore size, Merck). The blot transfer buffer consisted of 25 mM Tris ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by wet-transfer onto a Immobilin®-Fl polyvinylidene difuoride membrane with a 0.22 µm pore size (Merck) at 100 V for one hour at 4°C ...
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bioRxiv - Microbiology 2022Quote: Nasal sIgA was obtained from 1 ml nasal swab eluates by centrifugation (1 minute, 10 000 xg) and supernatant was sterile-filtered through a 0.4 μm pore (Merck). We determined sIgA concentrations by ELISA (Human IgA ELISA kit ...
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bioRxiv - Biochemistry 2022Quote: ... aliquots of 4 mL of the culture were vacuum-filtrated using 0.45 μm pore size filters (HVLP02500, Merck Millipore). The filters were transferred to -20°C cold 40:40:20 acetonitrile:methanol:water for metabolite extraction ...
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bioRxiv - Bioengineering 2022Quote: ... were centrifuged at 5000 g for 10 min and the supernatants were filtered (22μm pore size Millex-GP Syringe Filter Unit, Merck) and frozen at −20°C for measuring medium composition ...
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bioRxiv - Cell Biology 2023Quote: ... All samples were then filtered in a 47 mm polytetrafluoroethylene polymer (PTFE) membrane (0.45 μm pores, Merck Millipore, USA) and rinsed three times with filtered ultra-pure water ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 L of the water was filtered using a Sterivex filter cartridge with a nominal pore size of 0.45 μm (SVHV010RS, Merck) and a pump (Aqua Loader AL898 ...
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bioRxiv - Microbiology 2024Quote: ... Trans-well experiments were performed as described above in 24 well plates using inserts with a 0.4 µm pore size (Merck). In order to assess host cell susceptibility to small pore forming toxins ...
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bioRxiv - Biochemistry 2024Quote: ... cell culture supernatant was harvested by centrifugation at 3000 x g for 10 min and then filtered using 0.45 µM pore size filters (Merck). The supernatant was supplemented with 10 mM imidazole prior to purification using a HisTrap HP ...
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bioRxiv - Cell Biology 2024Quote: ... A Bio-Rad wet transfer system was used for immunoblotting onto PVDF membranes (Immobilon-P, 0.45-μm pore size, IPVH00010, Merck) before incubation with appropriate primary and secondary antibodies in 5% skimmed milk ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the nanodisc-embedded complex were pooled and concentrated on a 30 kDa cut-off ultra-filtration device (Amicon Ultra, Merck Millipore). For the reconstitution of radioactive nanodisc-embedded MlaF(His-E)B and MlaF(His-E)DB complexes ...
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bioRxiv - Molecular Biology 2023Quote: ... Complex containing fractions were pooled and concentrated again using Amicon Ultra – 0.5 mL units (50 kDa MWCO, Merck Millipore, Darmstadt, Germany).
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bioRxiv - Molecular Biology 2022Quote: ... Peak fractions containing the Swi2-Swi5 complex were concentrated using an Amicon Ultra-4 (50 k) centrifugal filter (UFC805008, Merck Millipore). After cleavage ...
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bioRxiv - Biochemistry 2024Quote: ... The appropriate fractions were pooled and the complex was concentrated to ∼ 9 mg/ml using Amicon UltraCel 10K MWCO centrifugal filters (Merck Millipore).
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bioRxiv - Cell Biology 2021Quote: ... antibody complexes were purified by incubation of lysate with Protein A Sepharose® beads (Merck, GE17-5138-01) for 3h rotating at 4°C ...
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bioRxiv - Neuroscience 2022Quote: Rabies injected brains were immunohistochemically stained using antibodies against the 2A linker protein (Merck/ Millipore, ABS31, 1:2000) found in tTA expressing cells ...
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bioRxiv - Cell Biology 2024Quote: ... RSV F-protein was detected using a mouse anti-RSV antibody used 1:5000 in casein (MAB8262, Merck), which was incubated at 37 °C ...
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bioRxiv - Microbiology 2020Quote: Live cell fluorescence imaging was performed as previously described 78 with the minor modification of nuclear staining with Hoechst (Merck) instead of DAPI at a final concentration of 5 μg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... Then 500 μL Nuclear RNA wash buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2, 1% BSA (Merck, #B9000S), 0.1% Tween (Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... cell pellets were resuspended in 500 μL Nuclear RNA lysis buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2, 1% BSA (Merck, #B9000S), 0.1% Tween (Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 2 was determined by staining with the DNA dye Hoechst 33258 (861405, Merck) as described previously (Saga et al. ...
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bioRxiv - Biophysics 2020Quote: ... 105 cells were seeded on the upper chamber of 24 well plate cell culture inserts containing 3 µm pores (Cat # 353096, Merck). The inserts were coated with rat-tail collagen I ...
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bioRxiv - Cancer Biology 2020Quote: ... 4°C) and the EV-depleted supernatant was filtered through a 0.22 μm pore PVDF Millex-HV filter (Merck Millipore) and analysed by NTA ...
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bioRxiv - Cell Biology 2021Quote: ... and were transferred at 100 mA for 70 min onto PVDF membrane (Immobilon-P, 0.45 μM pore size, Merck Millipore) using semi-dry blotting system ...
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bioRxiv - Biophysics 2020Quote: ... 50 μL of sample was injected onto a Discovery BIO Wide Pore C18 column (15 cm x 4.6 mm, 5 μm column with a guard column) (Supelco, Merck, UK) and eluted on a gradient of 95% water + 0.1% acetic acid and 5% acetonitrile + 0.1% acetic acid to 5% water + 0.1% acetic acid and 95% acetonitrile + 0.1% acetic acid at a 0.8 mL/min flow-rate over 40 mins ...
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bioRxiv - Microbiology 2020Quote: ... after which the filtrate was filtered onto 0.2 µm pore size (free-living cells) polycarbonate filters (Merck Millipore, Darmstadt, Germany). Foam from the Timor Sea (Station 8 ...
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bioRxiv - Cell Biology 2021Quote: ... Denaturated and fully-reduced proteins were resolved on Tris-glycine SDS-PAGE followed by western blot analysis onto PVDF membrane (Immobilon-P, 0.45 μM pore size, Merck Millipore) using enhanced chemiluminescence to detect bound antibodies (Pierce) ...
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bioRxiv - Biochemistry 2021Quote: ... adjusted to pH 5.8 using 3 M HCl and filtered with 0.2 μm nylon membrane filters (GNWP04700, 0.2 μm pore size, Merck Millipore Ltd.), while mobile phase B consisted of 100% methanol ...
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bioRxiv - Biochemistry 2021Quote: ... adjusted to pH 8 using 3 M HCl and filtered with 0.2 μm nylon membrane filters (GNWP04700, 0.2 μm pore size, Merck Millipore Ltd.), while mobile phase B consisted of 100% methanol (method described in Table 2) ...
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bioRxiv - Cancer Biology 2020Quote: ... Gaithersburg, MD, USA) and 8-μm-pore polycarbonate membranes (Nucleopore, Pleasanton, CA, USA) coated with 10 μg/ml fibronectin (Merck) as described elsewhere (Prevete et al. ...
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bioRxiv - Microbiology 2023Quote: ... These samples were filtered onto 5.0 µm pore-size polycarbonate filters (diameter, 25 mm; Isopore, Sigma-Aldrich, Merck, Darmstadt, Germany) using a filter holder (1225 Sampling Manifold Chambers ...
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bioRxiv - Cancer Biology 2023Quote: ... Conditioned medium containing recombinant lentivirus was collected 48hrs after transfection and filtered through non-pyrogenic filters with a pore size of 0.45 μm (Merck Millipore). Samples of these supernatants were applied immediately to target cells together with Polybrene (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Cultivation medium was first clarified by centrifugation for 5 min at 1500 x g and subsequent filtering through syringe mounted PVDF filter with 0.45 µm pore size (Merck Millipore). VLPs were pelleted through a 20% sucrose cushion at 125,000 x g for 120 min ...
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bioRxiv - Cell Biology 2023Quote: ... the reaction mixture was applied onto a GHP membrane of a Pall Nanosep® MF Centrifugal Device (pore size 0.45 µm, Merck) loaded with a 100 mg/ml Biogel P-10 Gel (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... all the samples were filtered through a 47 mm polytetrafluoroethylene polymer (PTFE) membrane with a 0.45 μm pore (Merck OmniporeTM) and rinsed three times with filtered ultrapure water ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was filtrated using Millex® HV membrane filter units with a pore size of 0.45 µm (Merck Millipore). Fy-4 was subsequently purified combining Ni-NTA affinity chromatography (HisTrap FF column ...
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bioRxiv - Genetics 2023Quote: ... and 0.2 g silica gel (High purity grade, pore size 60Å, 230-400 mesh particle size, Merck, KGaA, Darmstadt, Germany) containing 10 % pulverized AgNO3 (99.7% ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant of the transfected cells was collected 48 h later and filtered through a 0.45 μm pore-size filter (Merck Millipore). For viral infection ...
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bioRxiv - Microbiology 2024Quote: All phages in this study were isolated from wastewater according to the following procedure: 50 ml of wastewater was filtered through a 0.45 µm pore filter (Merck, Germany) to remove bacteria ...
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bioRxiv - Microbiology 2024Quote: ... A 5 mL of the fixed co-culture was filtered on a polycarbonate membrane filter with 0.1 μm pores (VCTP, 25mm, Merck Millipore). Cells trapped on the filter were washed by passing through 500 μL of pure water five times ...
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bioRxiv - Physiology 2024Quote: Neonatal mice were euthanised by decapitation and the ovaries were dissected from the bursa and were transferred into 2 µL media droplet on a transwell insert (0.4μm pore size, Millicell, Merck, Cat# PIHP01250) floating on 0.5 mL of DMEM/F-12 media ...
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bioRxiv - Neuroscience 2024Quote: ... by trituration with glass Pasteur pipettes and by subsequent filtering through a nylon gauze (pore size 140 µm; Merck, U.S.A.). Dissociated single cells were seeded on laminin (19 µg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... The TEV cleaved complex was concentrated to around 500 μl using a 4 ml Amicon® Ultra centrifugal filter concentrator (Merck Millipore) with a membrane molecular weight cut off 10 kDa ...