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Citations for Merck :
301 - 350 of 1343 citations for Eukaryotic Peptide Chain Release Factor GTP Binding Subunit ERF3A GSPT1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... the reduced peptide solution was purified five times using ZipTip Pipette Tips with C18 resin and 0.6 µl bed volume (Merck,) and eluted in 32.5% ACN/0.2% TFA ...
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bioRxiv - Plant Biology 2023Quote: ... TF complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore F4799). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher 88843 ...
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bioRxiv - Neuroscience 2022Quote: ... was used in a concentration of 20 µM and autocamtide-2 related inhibitory peptide II (AIP-II; Merck Millipore) at 4 µM.
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bioRxiv - Cell Biology 2023Quote: ... and were treated with various concentrations of peptides (as determined by prior Hoechst 33258 staining in serum–free media) or solvent (DMSO, 0.1% v/v, Merck) or positive control TGF-β1 10ng/ml (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... digested peptides were acidified by the addition of 5% (v/v) LC-MS grade Formid Acid (FA) (Merck, 5.33002.0050) and purified on C18 columns (Stagetips ...
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bioRxiv - Biochemistry 2024Quote: The monomeric recombinant peptide was prepared by resuspending it in a solution of 1% NH4OH (Merck Cat. No. 221228) in PBS buffer at a concentration of 1 mg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... EnSC were seeded at a clonal density of 30 cells/cm2 on 1 mg/ml fibronectin-coated 6-well plates and cultured in 10% DMEM/F12 medium supplemented with basic fibroblast growth factor (bFGF; 10 ng/ml; Merck Millipore, Watford, UK) for 10 days with a 50 % media change on day 7 ...
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bioRxiv - Neuroscience 2023Quote: ... after testing multiple antibodies (anti-NeuN rabbit Antibody, ABN78 & ABN78C3, Merck; anti-NeuN rabbit Antibody, ab177487, Abcam; anti-NeuN mouse Antibody, MAB377, Merck) and increasing antibody concentrations (up to 1:50) ...
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bioRxiv - Biophysics 2024Quote: ... Primary anti-KI67 antibody (Merck, rabbit polyclonal antibody) was diluted 1:300 in antibody buffer (0.2 % Tween 20 ...
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bioRxiv - Systems Biology 2020Quote: ... The resultant pellets were resuspended in 3% acetonitrile + 0.1% trifluoroacetic acid and peptide quantification performed using the Direct Detect system (Merck Millipore). Protein samples were normalized then vacuum concentrated in preparation for mass spectrometry.
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bioRxiv - Immunology 2023Quote: 200 μg of HLA.A2-peptide complex in PBS was concentrated to 1 mg ml−1 with the use of Amicon Ultra Centrifugal Filters (Merck), adjusted to pH 8.3 by adding freshly prepared NaHCO3 (0.1 M final concentration ...
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bioRxiv - Immunology 2022Quote: ... The mix was transferred to a plate coated with avidin and the FLAG-peptide was detected with an anti-FLAG-alkaline phosphatase conjugate (Merck), developed with pNPP SigmaFAST (Merck ...
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bioRxiv - Biophysics 2024Quote: ... The complex was then eluted off with the elution buffer (buffer system, 0.003% GDN and 150 μg/mL 3X FLAG® peptide (Merck)) ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies: anti-LC3 antibody Atg8 (Merck Millipore #ABC975) (1:1000) ...
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bioRxiv - Immunology 2020Quote: ... Peptides were separated from the HLA molecule remnants by ultrafiltration employing 3 kDa and 10 kDa Amicon filter units (Merck Millipore) for HLA-I and HLA-II ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then resuspended in 10 μl of 0.1% trifluoroacetic acid and tryptic peptides were desalted using a Zip-Tip® column (Merck Millipore) pipette tip according to the manufacturer’s protocol and dried in a vacuum concentrator before analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
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bioRxiv - Cancer Biology 2024Quote: ... a cassette coding for tdTomato with an N-terminal nuclear localization sequence and lack of a stop codon and a puromycin cassette with an N-terminal self-cleaving T2A peptide were amplified by KOD polymerase (Merck, 71085) and subjected to Gibson assembly.
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bioRxiv - Systems Biology 2024Quote: ... the acidified peptide solution was cleaned-up on self-packed StageTips (70) accommodating four discs of Empore C18 material (Merck Supelco). The material was prepared by consecutive application of pure ACN (Biosolve Chimie) ...
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bioRxiv - Biochemistry 2023Quote: All peptides used in this study probes were designed as 11-mers bearing biotinylated PEG2 linker in the N terminus and an amide group in the C terminus and were synthesized using Fmoc-solid-phase peptide chemistry with Novasyn TGR resin (Merck Millipore) as described in the Novabiochem Innovations 01/11 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibody (Merck guinea pig anti-NeuN 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... glial glutamate transporter GLT1 (guinea pig, polyclonal, synthetic peptide from the C-terminus of rat GLT1, Merck, AB1783, AB_90949, dilution 1:1,000), GFP (chicken ...
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bioRxiv - Plant Biology 2021Quote: ... 1000 μl tips were fitted with 2 plugs of C18 octadecyl 47mm Disks 2215 (Empore™) material and 10 μg of LiChroprep® RP-18 peptides (Merck). Tips were sequentially equilibrated with 100 % methanol ...
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bioRxiv - Plant Biology 2021Quote: ... Extracted peptides were dried in a speed-vac and resuspended in (v/v) 2% acetonitrile/0.2% trifluoroacetic acid (Merck, catalog no. 302031). A total of four biological replicates for each sample type was submitted.
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bioRxiv - Plant Biology 2023Quote: ... Pulldowns were done as described17 with the following modifications: The biotinylated Aux/IAA7 DII peptide (AKAQVVGWPPVRNYRKN, N-terminal biotin) was bound to streptavidin-agarose beads (Merck, #16-126). 250 μl of total protein extract was combined with 45 μl of DII-beads and either 0 μM or 10 μM cvxIAA or 10 μM IAA ...
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bioRxiv - Neuroscience 2023Quote: ... after testing multiple antibodies (anti-NeuN rabbit Antibody, ABN78 & ABN78C3, Merck; anti-NeuN rabbit Antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used were mouse anti-EGFR antibody (#GR01; Merck Millipore), goat anti-EEA1 (#sc-6414 ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibody RNA-DNA hybrid (s9.6) antibody (Merck Millipore, #MABE1095, 3ul) or GFP (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... We used the following primary antibodies: rabbit antibody against Protein Kinase Cα diluted at 1:500 (Antibody Registry ID: AB_477345, Merck), rabbit antibody against Recoverin at 1:1000 (AB_2253622 ...
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bioRxiv - Plant Biology 2020Quote: ... the ORF of CLEL6 lacking the predicted signal peptide was amplified by PCR from a previous construct and cloned into the NcoI restriction site of pETDuet1 (Novagen/Merck KGaA, Darmstadt, Germany). Correct orientation and translational fusion with the C–terminal His-tag was verified by sequencing ...
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bioRxiv - Plant Biology 2020Quote: ... ORFs of PSK1 and RGF1 lacking the predicted signal peptides were amplified by PCR from cDNA and cloned into the NcoI restriction site of pETDuet1 (Novagen/Merck KGaA, Darmstadt, Germany). C-terminal His tags were added by including 6 His codons in the reverse PCR primers ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated with the primary antibodies (see the list of antibodies in Table S2) and with an anti-rabbit secondary antibody (Merck), and visualized by chemiluminescence substrate (Merck ...
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bioRxiv - Neuroscience 2022Quote: ... antibodies (α-GluA2, Merck clone 6C4 ...
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bioRxiv - Immunology 2020Quote: ... antibodies in RPMI (Merck), containing 10% Heat Inactivated FCS (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies (NaV1.1 Merck, AB5204 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Immunology 2020Quote: Following protein digestion a 5% volume of the peptide solution was cleaned up with a reverse phase (C18) ZipTip (Merck Millipore, Burlington, MA, USA). The peptide sample was acidified with the addition of 1 μl 1M hydrochloric acid ...
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bioRxiv - Neuroscience 2021Quote: ... The secondary antibody (Goat anti-guinea pig IgG antibody-HRP, Merck, 1:1000) was incubated at RT for 2 h in TBS-T with 1 % dry milk powder ...
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bioRxiv - Neuroscience 2021Quote: ... primary antibody and with mouse monoclonal anti-map2 primary antibody (Merck Millipore, MAB3418). The secondary antibodies used were the anti-Rabbit Alexa Fluor 546 (Thermo fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies included: goat anti-mouse IgG antibody (Merck Millipore AP308P, (H+L) HRP conjugate ...
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bioRxiv - Microbiology 2024Quote: ... suis antibody (1:100) and a mouse anti-tubulin beta III antibody (Merck Millipore ...
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Synaptic inhibition in the accessory olfactory bulb regulates pheromone location learning and memorybioRxiv - Neuroscience 2024Quote: ... Primary antibody incubation (1:1000 ul of anti-Chicken NeuN antibody, ABN91, Merck in 7.5% NGS,2.5% BSA in 1% triton and 1 X PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... or α-H3K4-me3 antibody (Rabbit monoclonal antibody (#04–745, clone MC315; Merck Millipore). DNA was purified by phenol-chloroform extraction and ethanol precipitation ...
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bioRxiv - Neuroscience 2020Quote: Primary antibodies used: anti-NeuN (1/1000) mouse monoclonal antibody (MAB377, Merck Millipore, USA), anti-CamKII (1/100 ...
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bioRxiv - Biochemistry 2020Quote: ... as primary antibodies and HRP conjugated anti-mouse or anti-rabbit secondary antibodies (Merck).
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bioRxiv - Neuroscience 2021Quote: ... the following antibodies were used: rabbit anti-FLAG antibody (Merck Millipore cat. no. F7425), mouse anti-neurofilament 70 kDa antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies guinea pig polyclonal anti-doublecortin antibody (Merck, AB2253, 1:200 dilution), mouse monoclonal anti-CKAE1/AE3 (Dako ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies used included mouse monoclonal antibodies against Tau-1 (1:1000; AB5622, Merck) or α-tubulin (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... After overnight incubation with primary antibodies (mouse anti-Fibroblasts Antibody, clone TE-7 (Merck, Cat# CBL271 ...
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bioRxiv - Neuroscience 2020Quote: ... antibody against STIM1 from Merck Millipore (ref AB9870) ...
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bioRxiv - Neuroscience 2020Quote: ... Antibodies: PSD95 (Merck - MABN 68) and Synaptophysin 1 (Synaptic Systems 101 004) ...