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Citations for Merck :
251 - 300 of 1343 citations for Eukaryotic Peptide Chain Release Factor GTP Binding Subunit ERF3A GSPT1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... Peptide fractions were eluted using MeOH buffer containing trifluoroacetic acid (TFA) (Merck Sigma, Cat. No. 302031), FA or ABC ...
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bioRxiv - Immunology 2022Quote: ... The proteolytically cleaved peptides were extracted and desalted using ZipTip μC18 tips (Merck Millipore, Darmstadt, Germany). The peptide lysates were re-suspended in 15 µl 0.1% formic acid and analysed by nanoliquid chromatography mass spectrometry (UltiMate 3000 RSLCnano ...
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bioRxiv - Biophysics 2022Quote: ... Both recombinant and synthetic lyophilized peptides were dissolved in 6M guanidine thiocyanate (Merck KGaA, Darmstadt, Germany) and incubated overnight at 1250 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were eluted with 50 μl of 100 μg/mL of 3X FLAG tag peptide (Merck) in solubilization buffer followed by acetone precipitation (overnight ...
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bioRxiv - Cell Biology 2023Quote: ... pH 2.0) for endogenous MDH2 IP and incubation with 250 μg/ml 3XFLAG peptide (Merck F3290) in the case of FLAG IP ...
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bioRxiv - Microbiology 2024Quote: ... The digestions were dried under vacuum and peptides were desalted using ZipTips C18 microcolumns (Merck Millipore). Desalted peptides were vacuum dried and resuspended in 0.1% formic acid (FA).
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bioRxiv - Biochemistry 2024Quote: Manual peptide synthesis was performed on Rink Amide MBHA resin (0.52-0.65 mmol/g loading, Merck). Couplings were carried out by adding HATU (4 eq ...
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bioRxiv - Molecular Biology 2020Quote: ... From the remaining nuclei RNA-IP was then undertaken with the MagnaRIP Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit protocol (17-700, Merck) from step I.3 ...
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bioRxiv - Cell Biology 2022Quote: ... in 100µl of binding/wash buffer supplemented with protease inhibitor with protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore) and phosphatase inhibitors ...
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bioRxiv - Cancer Biology 2023Quote: ... the solution was filtered using a sterile syringe filter coupled to a filter unit with a low protein binding Durapore PVDF membrane (Merck Millipore) and the solution was poured into an amber tube to protect from light exposure.
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bioRxiv - Plant Biology 2022Quote: The m6A enrichment experiment was performed as described previously with minor modifications (Dominissini et al., 2013) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck) was used following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: SFPQ and FLAG RIPs were performed in TREx-BCBL-1 RTA cells using EZ-Magna RIP RNA binding Immunoprecipitation Kit (Merck Millipore) as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... the medium was replaced with a serum-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days in the BDNF-containing media ...
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bioRxiv - Biophysics 2020Quote: ... the cells were incubated with a FCS-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days ...
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bioRxiv - Neuroscience 2021Quote: ... The cell medium was aspirated from the wells and fresh medium with one of the factors (Bafilomycin, 200 nM, Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: HPAECs were seeded at the density of 10,000 cells/well in a pre-prepared 96-well plate containing 30μl of growth-factor reduced Matrigel (Merck, # CLS354230-1EA) per well (38) ...
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bioRxiv - Neuroscience 2023Quote: ... Once DRGs were removed and placed in ice-cold neuronal growth medium (Neurobasal medium, B27, and 10 ng/ml Nerve growth factor (NGF)-2.5 s (Merck Millipore), 2 mM L-glutamine ...
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 2.5 µg mL-1 α-factor grown at 25°C (Cat. No. T6901, Merck). After 90 min ...
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bioRxiv - Microbiology 2024Quote: ... removed and replaced with infection media −50:50 Ham’s F-12 Nutrient Mix and DMEM GLUAMax supplemented with 10 ng/ml Epidermal Growth Factor (Merck SRP3027), 5 µg/ml Insulin (Merck I2643) ...
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miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... Labelled peptides were separated on Chromolith Caprod RP-18e HR capillary column (150 × 0.1 mm; Merck Millipore) using linear gradient of buffer B (98% ACN and 0.05% TFA in water ...
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bioRxiv - Cell Biology 2020Quote: ... and proteins were eluted using 100 µl of 150 ng/µl 3× FLAG Peptide (F4799, Merck KGaA) or HA peptide (HY-P0239 ...
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bioRxiv - Microbiology 2022Quote: ... 100 µg of the dried peptides were dissolved in 90 µl of 0.2 M EPPS buffer (MERCK)/10% acetonitrile ...
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bioRxiv - Immunology 2020Quote: ... MHC–peptide complexes were eluted by repeated addition of 0.2% trifluoroacetic acid (TFA, Merck, Whitehouse Station, NJ). Peptides were purified by ultrafiltration using centrifugal filter units (Amicon ...
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bioRxiv - Biochemistry 2023Quote: ... the peptides were desalted and intact glycopeptides were enriched by ZIC-HILIC beads (Merck, Product no. 1.50458.0001) according to our previous study[25].
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bioRxiv - Biochemistry 2021Quote: RNA immunoprecipitation (RIP) assays were conducted using the EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck Chemicals (Shanghai Co., Ltd). The anti-YTHDC1 antibody for RIP was purchased from Cell Signaling Technology ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal tissues from P40 WT mice were collected and lysed with IP lysis buffer in EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck-Millipore). YTHDF2 (Proteintech ...
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bioRxiv - Biochemistry 2024Quote: ... MP5.5 and MP5.6 for binding to bovine α-chymotrypsin (PT5) were conducted in the presence or absence of phenylmethylsulfonyl fluoride (Merck, Darmstadt, Germany) and aprotinin (Cytiva ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Each lane was excised and cut into approximately 1 mm x 1 mm pieces using a sterile scalpel and transferred into one well of a 96 well hydrophilic low protein binding filter plate (Merck Millipore, MSBVN1210). Gel pieces were destained using destaining buffer (50% 50 mM ammoniumbicarbonate buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Next the medium was replaced with a serum-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days in the BDNF-containing media ...
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bioRxiv - Molecular Biology 2021Quote: ... was centrifuged at 1500 rpm for 10 mins and the supernatant was used to measure inflammatory cytokines (IL-4, IL-5, IL-13, γ-INF) by LUMINEX multi-factor detection (MERCK). The cell pellets were fixed in 4% formaldehyde for Wright-Giemsa staining and total cells were counted and classified in each slide.
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bioRxiv - Molecular Biology 2021Quote: TEER measurements [22] were performed on 3rd day of incubation with growth factors and (or) different inhibitors by using Millicell ERS-2 Electrical Resistance System (Merck-Millipore). Each insert was measured in three different locations ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine quantification was performed using a Human Cytokine/Chemokine/Growth Factor Panel A 48-Plex Premixed Magnetic Bead Multiplex Assay (Merck Millipore), using the Luminex MAGPIX System in 96-well plate format ...
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bioRxiv - Developmental Biology 2023Quote: ... 80% of complete medium was changed to either new complete medium without trophic factors or complete medium containing 5 ng/mL BDNF (GF029 Merck Millipore) or 100 ng/mL human PGRN (AG-40A-0188Y Adipogen) ...
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 3.0 µg mL-1 α-factor at 30°C (Merck, Cat. No. T6901). After 90 min ...
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bioRxiv - Plant Biology 2021Quote: ... Protein complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: Peptides EcFtsN1-32-C and VmFtsN1-29-C were labelled with maleimide-Atto 495 (Merck Life Science UK). EcFtsA1-405 and VmFtsA1-396 were buffer exchanged into binding buffer (50 mM HEPES/KOH ...
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bioRxiv - Microbiology 2021Quote: Peptides EcFtsN1-32-C and VmFtsN1-29-C were labelled with maleimide-Atto 495 (Merck Life Science UK). EcFtsA1-405 and VmFtsA1-396 were buffer exchanged into binding buffer (50 mM HEPES/KOH ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were dissolved in 2% acetonitrile containing 0.1% trifluoroacetic acid and desalted using C18 ZipTips (Merck Millipore, Germany). Each sample was independently analysed on a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... The peptides were diluted to 50 µg/mL in 10 mM sodium acetate pH 5 (Merck, Darmstadt, Germany) and injected overflow cell two of each channel to a final signal of 3400 RU for L-CDP1 and 1300 RU for L-CDP7 ...
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bioRxiv - Microbiology 2024Quote: ... and subsequently eluted in 150 μL of wash buffer with 500 ng/μL FLAG peptide (Merck, Darmstadt, Germany) by rotation on a rotator for 2 h at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Phospho peptides were then reconstituted in 10 μl of MS loading buffer containing 0.3% Trifluoroactetic acid (TFA)(Merck) and 2% Acetonitrile (ACN ...
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bioRxiv - Pathology 2022Quote: ... The elution fraction containing His6-PhoP was dialyzed in Binding Buffer to remove the high concentration of imidazole and concentrated using an ultrafilter (Merck KGaA, Darmstadt, Germany). SDS-PAGE and Western blot with an anti-His tag antibody was used to confirm the purified protein (ABclonal ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were seeded in culture dishes and the specific pharmacological inhibitors radicicol (inhibitor of ATP-binding site of Hsp90, Sigma-Aldrich, Merck, Darmstadt, Germany), cyclosporine A (inhibitor of Cyp activity ...
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bioRxiv - Plant Biology 2024Quote: ... and then clarified via passing through Millex® -GP Fast Flow & Low Binding Millipore Express® PES Membrane 0.22 µm syringe filter unit (SLGP033RS, Merck Millipore, USA).
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bioRxiv - Genomics 2022Quote: ... followed by quenching with final 1 x Glycine solution for 5 min at RT utilizing the Magna Nuclear RIP (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit (Merck millipore, 17-10520). Cross-linked cells were then centrifuged at 800 x g at 4°C and washed three times with ice cold DPBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 250 ng of pTOPflash (wt-TCF) or pFOPflash (mutated-TCF binding site, negative control) reporter plasmids (TOPflash luciferase reporter assay, Merck Millipore, Darmstadt, Germany) were transfected with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Synthetic Biology 2022Quote: ... the supernatant was sterile filtered (0.22 μm) and afterwards concentrated by the factor of 20 using 100 kDa molecular weight cut-off filters (Merck KGaA, Darmstadt, Germany). To determine the number of released vesicles ...
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 3.0 µg mL-1 α-factor grown at 25°C (Merck, Cat. No. T6901). After 90 min ...
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bioRxiv - Immunology 2021Quote: ... (final concentration of 2µg/mL) and pools of original or beta variant peptides on hydrophobic-PVDF ELISpot plates (Merck) coated with 5µg/mL anti-mouse IFNγ (AN18) ...
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bioRxiv - Systems Biology 2021Quote: ... Peptides were eluted in a final volume of 600 μL and filtered with 0.22 μm PVDF filters (Merck Millipore) before dried in a centrifugal vacuum concentrator ...