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Citations for Merck :
301 - 350 of 4636 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 4 g PFA (Merck) was dissolved in 80 ml PBS heated to 60 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Germany)/ 4% paraformaldehyde (Merck, Germany) in 0.05 M cacodylate buffer pH 7.4) ...
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bioRxiv - Cell Biology 2023Quote: ... 4-OHT (Merck, #H6278) was dissolved in ethanol at 1 mM and used at a final concentration of 0.5 μM in the culturing medium.
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bioRxiv - Developmental Biology 2024Quote: ... Benzonase (Merck, 70746-4)-treated biotinylated protein samples suspended in 1 mL RIPA buffer were incubated overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... individual COs were transferred to an Eppendorf tube containing CMM medium with 1:100 of virus containing medium and 5µg/ml Polybrene (Merck; TR-1003-6) and incubated over-night ...
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bioRxiv - Plant Biology 2023Quote: ... Sections were rinsed 6 times in MTSB and incubated with 1 µg/mL Calcofluor white (Fluorescent Brightener 28 disodium salt solution, Merck, in MTSB). After rinsing ...
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bioRxiv - Cancer Biology 2024Quote: ... The extracted 212Pb in 0.1 M HCl obtained from the emanation generator was adjusted to pH 5-6 with 5 M sodium acetate (Merck, Darmstadt, Germany) and mixed with TCMC-mAbs with specific activities of 1-50 MBq/mg ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Neuroscience 2021Quote: RNAs with sequences 5’-AAGGAUGGAUGGAG-3’ (healthy) and 5’-AAGCAUGGAUGGAG-3’ (risk) were synthesised by Merck, resuspended in Ultrapure water ...
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bioRxiv - Plant Biology 2021Quote: Arabidopsis 14-3-3 isoforms Epsilon and Lambda were expressed using the pGEX-4T1 vector (Merck), as a translational fusion with glutathione-S-transferase (GST ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated overnight at 4°C with anti-KCC2 (1:200; Merck Life Sciences, Italy, #07-432) or anti-IGF-1R β (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... was cultured in high-glucose (4.5 g × ml−1) Dulbecco’s modified Eagle’s medium (DMEM) containing 4 mM stable glutamine (Merck) and supplemented with 6% inactivated fetal calf serum (iFCS ...
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bioRxiv - Neuroscience 2020Quote: ... samples were incubated overnight at 4°C with primary antibodies against puromycin (1:500, MABE343, Merck Millipore), βIII tubulin (1:500 ...
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bioRxiv - Immunology 2020Quote: ... the cells were further cultured for 4 days in the same conditions plus Dexamethasone (1 μM; MERCK). IDCs were equally generated in 4 days ...
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bioRxiv - Cancer Biology 2024Quote: ... and secondary antibody anti-rabbit (CST, 1:3000) diluted in PBS containing 4% skim-milk powder (Merck) and 0.2% Tween 20 (Sigma).
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bioRxiv - Biochemistry 2024Quote: ... and 1 mM ethylenediaminetetraacetic acid (EDTA)) using an ultra-centrifugal filter (Amicon ultra-4 [30 kDa]; Merck). The (His)6-tag was cleaved by reacting with HRV3C overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... and 1x phosphatase inhibitor 3 (Merck). Cell debris was removed by pelleting at 5000g for 10mins ...
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bioRxiv - Immunology 2021Quote: ... 3-Methyladenine (3MA, 5mM; Merck Millipore) or Anakinra (500ng/ml ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-mGluR2/3 (Merck Millipore), rabbit anti-CRTAC1 (Merck Millipore) ...
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bioRxiv - Neuroscience 2022Quote: ... primarily fixed in 3% glutaraldehyde (Merck) in 0.1 M sodium phosphate buffer with pH 7.2 at 4°C for 1 h ...
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bioRxiv - Physiology 2024Quote: ... - 3% horse serum (Merck, ref H1270) PBS solution for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... 3 units/ ml nystatin (Merck: N1638) and 20 mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3% bovine serum albumin (Merck). The azide-alkyne reaction was performed using the Click-iT™ Cell Reaction Buffer Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... nystatin (3 units/ml) (Merck: N1638) and ascorbic acid (500 μM ...
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bioRxiv - Neuroscience 2023Quote: ... DAPI (Merck; UK 28718-90-3) was included as a final wash stage ...
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bioRxiv - Developmental Biology 2024Quote: ... or 3% Rabbit Serum (R9133, Merck)) and finally applying the conjugated antibody ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.4) (final concentration: 12mg/mL) containing 5 μL Benzonase (final concentration: 1 μL benzonase/mL (MERCK, 71205-3). After dissolving ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with sgRNA lentiviruses at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours and then reprogramming was initiated by addition of reprogramming medium ...
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bioRxiv - Biochemistry 2021Quote: ... Appropriate fractions were concentrated to 3-0.7 mg mL−1 using 30,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Neuroscience 2024Quote: ... for 180 min prior to washing and development in Streptavidin-tertiary reagent (1:500 in 2% BSA and 0.1% Triton [TBS]) conjugated to either Cyanine 3 (Cy3) (Merck) or Alexa Fluor 488 (Life technologies ...
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bioRxiv - Immunology 2023Quote: ... the mice were intranasally sensitized using 1 μg of 2′3′-cyclic GMP-AMP (cGAMP, 531889; Merck Darmstadt, Germany) and 1 μg of (HDM ...
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bioRxiv - Cell Biology 2023Quote: ... 24×50 mm high-precision coverslips (no. 1.5H; Marienfeld) were cleaned in Piranha solution (3:1, 98% H2SO4 (Merck):30% H2O2 (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293T cells were transfected at ∼40 % confluency using a 3:1 polyethylenimine (branched, average Mw ∼25,000 Da; Merck) to DNA ratio (30:10 μg ...
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bioRxiv - Biophysics 2023Quote: ... Liposomes were labeled before experiments with 1% of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (PE-Rhodamine) (Merck) at a concentration of 10µg/mL.
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bioRxiv - Biophysics 2021Quote: ... before dissolving them finally in 6 M guanidine hydrochloride (Merck). H57-scFV were refolded from inclusion bodies by a stepwise reduction of the guanidine hydrochloride concentration within the refolding buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: Cell culture inserts for 6-well plate (Merck Millipore, #044003)
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bioRxiv - Biochemistry 2020Quote: ... An empty 6 mm antibiotic assay filter disc (Merck-Millipore) was then placed in the centre of the lawn and 10 μl of 1 M NaN3 was pipetted onto the filter disc ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were detached with 500 μl/6-well Accutase (Merck) for 3 minutes at 37 °C ...
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bioRxiv - Zoology 2022Quote: ... 6 μL 2X Kappa2G Fast PCR Ready Mix (Merck, Germany). Primer concentrations and volumes differed between the multiplex assays and are detailed in Table S1 ...
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bioRxiv - Genomics 2023Quote: ... then resuspended in 6 μL deionised formamide (Merck Life Science) per hybridisation and incubated in a shaker (1400 rpm ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... but a 6 mg/mL solution of bovine fibrinogen (Merck) was used instead of plasma ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 μM MβCD (lipid raft inhibitor; Merck, #128446-36-6). Rapamycin (MCE ...
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bioRxiv - Genomics 2023Quote: ... then immersed in an amino-silane solution (3% vol/vol (3-aminopropyl) triethoxysilane (Merck cat no. 440140), 5% vol/vol acetic acid (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... The loading reaction (480 µL) was spun in a pre-rinsed Amicon Ultra 0.5 mL Centrifugal Filter with 100 kDa MWCO (Merck Millipore, 6 000 g, 6 min). After two washes with water (480 µL ...
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bioRxiv - Immunology 2020Quote: ... Protein was concentrated to 1 ml using a Amicon Ultra-4 Ultracel 30 kDa spin concentrator (Merck Millipore) and the protein was separated on a HiPrep 16/60 Sephacryl S-200 HR column (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde for 30 min and stained with 1% crystal violet (Merck, Darmstadt, Germany) for 20 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated overnight at 4°C with monoclonal anti-NeuN antibody (1:500; Mouse, MAB377, Merck Millipore), in a 50% dilution of blocking buffer + 0.5% Triton X-100 ...
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bioRxiv - Cell Biology 2021Quote: ... 1% Triton X-100) and 20μg was immunoprecipitated overnight at 4°C with 10μg S9.6 antibody (Merck, MABE1095) conjugated to 100μL Pierce ChIP-grade protein-A/G magnetic beads (Thermo ...
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bioRxiv - Molecular Biology 2022Quote: ... CMCs were incubated at room temperature for 4 hours with rhodamine-labeled phalloidin (1:100, Merck Life Science) and/or Alexa-Fluor-488 ...
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bioRxiv - Molecular Biology 2022Quote: ... sections were incubated at room temperature for 4 hours with rhodamine-labeled phalloidin (1:100, Merck Life Science) and/or Alexa-Fluor-488 or -568-conjugated IgG secondary antibodies (1:500 ...