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Citations for Merck :
3301 - 3350 of 6398 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... cell culture medium was removed and cells fixed with 100μl of 4% para-formaldehyde (PFA) (Merck, London UK) for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: E10.5 embryos were fixed in 4% PFA/PBS for 1.5 h followed by incubation in 15% sucrose (Merck)/PBS for 2 h and overnight incubation in 30% sucrose/PBS ...
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bioRxiv - Biochemistry 2023Quote: ... at 4 °C for 30 s and concentrated in a 100 kDa Amicon Ultra centrifugal filter (Merck Millipore), which was equilibrated with 1% GDN in IP buffer ...
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bioRxiv - Cell Biology 2023Quote: ... resuspended in PBS containing 0.2μM DAPI and maintained at 4°C until measurement by IFC or mounted in Mowiol (Merck) on a microscopy slide.
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bioRxiv - Immunology 2023Quote: ... Tissue samples (200 mg) were digested in 4 ml of EMSURE ISO nitric acid 65% solution (Merck, #1.00456.2508) using MARS 6 microwave extraction system ...
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bioRxiv - Immunology 2024Quote: ... Cell supernatants were harvested 5 days post-transfection by centrifuging for 10 min at 4,000 g and 4 °C and sterile filtering through a 0.45 μm filter followed by a 0.22 μm filter (Merck). cOmplete mini EDTA-free protease inhibitor cocktail was added to the supernatants at 1 mg/mL (Roche).
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bioRxiv - Biochemistry 2024Quote: ... Coverslips were rinsed in distilled water and mounted using Mowiöl (12 ml of 0.2 M Tris buffer pH 7.5, 6 ml distilled water, 6 g glycerol, 2.4 g Mowiöl 4-88, Merck Millipore). Samples were imaged immediately or within the next 48 h ...
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bioRxiv - Biophysics 2024Quote: ... concentrated using 4 mL and 0.5 mL 30 kDa MW cut-off centrifugal filters (Amicon Ultra, Merck Millipore) to concentrations >1 µM ...
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bioRxiv - Biochemistry 2024Quote: ... Then the mixtures were concentrated to about 400 μM of protein concentration using Amicon Ultra-4 (Merck Millipore). Crystallization screening was performed using different commercially available crystallization screen by the sitting drop vapor-diffusion method at 4°C and 20°C ...
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bioRxiv - Biophysics 2024Quote: ... The urified protein was concentrated using an Amicon® Ultra-4 centrifugal filter unit – 3000 NMWL (Merck-Millipore).
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bioRxiv - Cell Biology 2024Quote: ... and mounted on grease-free slides using Mowiol- based mounting medium (12% Mowiol 4-88 (Merck, 81381-250G), 30% glycerol ...
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bioRxiv - Biochemistry 2024Quote: ... with the additional step that lysis was preceded by 4 h treatment with 10 μM MG132 (Merck, 4693159001) and 10 μM PR619 (Bio-Techne ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was clarified by ultracentrifugation at 55,000 g for 60 min at 4°C and filtered through 0.45 µm (Durapore Merck) filters ...
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CAP1 and cofilin1 cooperate in neuronal actin dynamics, growth cone function and neuron connectivitybioRxiv - Neuroscience 2020Quote: ... mouse anti tau-1 (1:200, Merck Millipore), mouse anti-c-Myc (1:200 ...
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bioRxiv - Molecular Biology 2020Quote: ... RAD51 (Merck, Ab-1, PC130, dilution 1:1000), γH2AX (Millipore ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-Arl13b (Sanbio, 17711-1-AP, 1/500) and anti-FANK1 (Merck, HPA038413, 1/50). After washing with TBST ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 mM MgCl2) before addition of Laemmli buffer with 0.25 U/µL benzonase (final concentration, Merck Millipore) for 10 min followed by 5 min denaturation at 95°C.
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bioRxiv - Immunology 2021Quote: ... The plates were finally washed 5 times with HSWB before adding 100 µL of TMB (Merck, Germany) to each well ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.2) and purified by filtration through 5 μm pore polycarbonate membranes (Merck Millipore Co. Cork, Ireland).
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bioRxiv - Cancer Biology 2022Quote: PEGylated-poly T coated coverslips were assembled as described and further passivated with 5% BSA (Merck, A7906) for 30 minutes followed by wash with IMB ...
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bioRxiv - Neuroscience 2022Quote: ... cells were washed three times with PBS and blocked with 5% normal donkey serum (NDS) (Merck-Millipore), 0.1% Triton X-100 in PBS for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... supplemented with 2.5 µM IWP4 (Stemgent, Cambridge, MA, USA) and 5 µM XAV939 (Merck, Kenilworth, NJ, USA). The culture medium was refreshed with RPMI□+□B27+insulin medium every other day ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was cleared via centrifugation and loaded on two 5 mL HiTrap Heparin HP (Cytiva, Merck) columns (connected in series ...
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bioRxiv - Neuroscience 2022Quote: Conditioned media from astrocytes were collected at 5 DIV and filtrated with Millex-GP filter (SLGPR33RS, Merck). ACM was concentrated with Amicon Ultra 10kDa (UFC501008 ...
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bioRxiv - Cell Biology 2022Quote: ... at a final concentration of 5 μg/ml in M2 medium or Latrunculin B (428020-1MG, Merck) at a final concentration of 5 μM in M2 medium (to disrupt F-actin) ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were blocked with 5% non-fat milk in PBS with 0.05% Tween-20 (Merck; blocking buffer) and incubated with primary antibodies (Supplemental Table 2 ...
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bioRxiv - Immunology 2020Quote: ... Lysates were further cleared by sterile filtration employing a 5 µm filter unit (Merck Millipore, Darmstadt, Germany). The first column contained 1 mg of W6/32 antibody coupled to sepharose ...
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bioRxiv - Immunology 2021Quote: ... pH 5 (0.1 M citric acid monohydrate from Sigma and 0.2 M disodium phosphate dihydrate from Merck)) was added to the plate and incubated for 30 minutes in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Metabolite separation was achieved by using a ZIC-pHILC column (150 x 4.6 mm, 5 μm; Merck) and Dionex UltiMate 3000 liquid chromatography system (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... At L4 larval stage animals were transferred to plates containing 10 µM of 5-Fluorouracil (Merck, #F6627) to prevent progeny production ...
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bioRxiv - Genetics 2023Quote: ... At L4 larval stage animals were transferred to plates containing 10 µM of 5-Fluorouracil (Merck, #F6627) to prevent progeny production ...
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bioRxiv - Molecular Biology 2023Quote: ... A total of 1.2 mL of a 10 mM sodium periodate solution (Merck, cat# 7790-28-5) was added to the suspension ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were then incubated with 10 ml blocking buffer (5% skim milk powder (Merck Millipore, 115363) in TBST ...
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bioRxiv - Cell Biology 2023Quote: ... The sections were treated with 0.5% Periodic acid for 5 min and stained in Schiff’s reagent (Merck) for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... 5% H2) at 37°C in Bacteroides minimal medium (BMM) containing 27.5 μM of iron (FeSO4, Merck)91 ...
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bioRxiv - Biochemistry 2023Quote: ... 250-mm long) using an acetonitrile gradient (5–35% acetonitrile for 160 min, Merck KGaA, Darmstadt, Germany) in the presence of 0.1% formic acid at a flow rate of 250 nl/min ...
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bioRxiv - Cancer Biology 2023Quote: PEGylated-poly(T)-coated coverslips were assembled as described and further passivated with 5% BSA (Merck, A7906) for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... 5 mg/ml Hemin (Mercury Cat#3741) and 5ul/ml vitamin K1 (Merck Cas#84-80-0)) under anaerobic conditions.
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bioRxiv - Microbiology 2023Quote: ... Plates were washed 5 times with PBS-T and 100µl of 3,3′,5,5′-Tetramethylbenzidine (TMB) (Merck Millipore) equilibrated at room temperature were added ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then cultured in G-IVF PLUS (Vitrolife, #10136) under mineral oil (Merck, # 8042-47-5) at 37°C in 5% O2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... twice in 100% ethanol for 5’ each followed by washing in Xylene (Sigma-Aldrich, Merck, Overijse, Belgium) twice for 5’ each ...
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bioRxiv - Biophysics 2023Quote: ... the actin was mixed with a 5-fold molar excess of NHS-XX-Biotin (Merck KGaA, Germany) and incubated in the dark for 15 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The heptose-phosphates were separated by a SeQuant ZIC-pHILIC (5 µm, 150×2.1 mm) column (Merck). Solvent A ...
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bioRxiv - Biophysics 2024Quote: ... mN2GCH was concentrated down to 500 μl using an Amicon Ultra 5 centrifugal filters (Merck, Darmdstad, Germany) and then injected onto a Superdex 200 10/300 GL column (Sigma-Aldritch ...
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bioRxiv - Immunology 2024Quote: ... slides were washed with PBS and counterstained with a Hoechst-34580 DNA dye (5 μg/mL; Merck) in some cases supplemented with combinations of UEA1 Atto 488-conjugated lectin (10 µg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... and treated for 24h with 10 μM Nutlin 3a and/or 5 μg/ml 17β-Estradiol (Merck). Four independent experiments were performed ...
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bioRxiv - Neuroscience 2024Quote: ... cells were permeabilized for 5 minutes with DPBS containing Triton 0,1% (X100, Merck Life Science, Milan, Italy). To prevent non-specific binding of the antibody ...
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bioRxiv - Microbiology 2024Quote: ... a stock citrate solution of 5 mg/mL was prepared by using tri-sodium-citrate dihydrate (MERCK). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... equal amounts of 100 μM 5’-Cy5 labelled forward strand and unlabelled reverse strand oligonucleotides (Evrogen/Merck) were mixed in the annealing buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were separated using mPAGE 12% Bis-Tris gels (Merck; 5 μl loading volume per sample) with MOPS running buffer (50 mM MOPS ...