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Citations for Merck :
2851 - 2900 of 3431 citations for Rat Interleukin 1 Receptor Antagonist IL1RN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Cells were harvested and lysed using RIPA lysis buffer (Beyotime, China) supplemented with 1% protease inhibitor cocktail (MERCK, Germany). After centrifugation at 14 ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Physiology 2019Quote: ... The hPCLS were treated with 10ng/ml recombinant human TGFß 1 protein (R&DSystems#240-B-010) and 25µM (in 750µl of DMEM) of GM6001 (Merck#CC1010). Lived dead staining was carried out using calcein-am/ ethidium homodimer kit (ThermoFisher#L3224 ...
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bioRxiv - Pathology 2021Quote: ... 96-well plate was coated with 1 μg/ml monoclonal anti-lubricin antibody clone 9G3 (Merck Millipore, Billerica, MA) in TBS at 4°C over-night ...
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bioRxiv - Microbiology 2021Quote: ... Isolated lipids were dissolved in 30 μL of chloroform:methanol (2:1, v/v) and 5 μL loaded on thin-layer chromatography (TLC) silica gel plates F254 (Merck). Lipids were separated in chloroform/methanol/water (20:4:0.5 ...
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bioRxiv - Microbiology 2019Quote: ... Reaction products were then visualized by spotting 1 μl on PEI-cellulose thin layer chromatography (TLC) plates (Merck Millipore) and separated using 1 M KH2PO4 ...
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bioRxiv - Genetics 2021Quote: ... a mouse anti-actin monoclonal antibody (#MAB1501R, used at 1:5,000 dilution for the Western blot analyses, Merck Inc.); a mouse anti-glyceraldehyde-3-phosphate dehydrogenase monoclonal antibody (#MAB374 ...
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bioRxiv - Cell Biology 2021Quote: ... Surfaces were functionalised by flooding the device with 1% (v/v) trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Merck) in HFE-7500™ (3M™) ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were passaged at 70 – 90% confluency using 1% PBS and trypsin/EDTA (0.25%,0.02%) in T75 tissue culture flasks (Merck, Australia) and cultured at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... pellets were resuspended in urea lysis buffer (8 M Urea, 50 mM Tris pH 7.5, and 1:200 freshly added protease inhibitor cocktail (Merck)) ...
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bioRxiv - Biochemistry 2021Quote: ... Appropriate fractions were concentrated to 3-0.7 mg mL−1 using 30,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Biochemistry 2021Quote: ... Purified ShTniQ was concentrated to 10 mg mL−1 using 10,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Cell Biology 2021Quote: ... cells were starved overnight in serum-free DMEM and then treated for 1 hour with 5 ng/ml TGFβ1 (Preprotech) or with 5 μM SB431542 (Merck), and intensities quantified by ImageJ ...
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bioRxiv - Biophysics 2020Quote: ... the purified mEGFP-tagged NbALFA was concentrated to 1 mg/ml using an Amicon Ultra 30K filter (Merck Millipore) and stored at -80 °C before use ...
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bioRxiv - Cell Biology 2020Quote: ... H4S1 phosphorylation was detected using the anti-phospho histone H4/H2A S1p antibody at 1:1000 (Upstate/Merck-Millipore); sf-GFP-Sps1 was detected using JL-8 anti-GFP antibodies (Takara/Clontech ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were washed twice with cytoskeleton buffer and stained with 1:500 30972 Abberior® STAR 635 (Merck) O/N at 4°C ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-mouse IgG HRP antibody (AP192P, Merck Millipore). Protein detection was performed using SuperSignal West Pico PLUS (34579 ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-rabbit IgG HRP antibody (AP182P, Merck Millipore) and 1:5000 dilution of mouse anti-GAPDH (ab8245 ...
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bioRxiv - Microbiology 2020Quote: ... NO2- concentrations were determined using colorimetric test strips (NO2--test, 0-10 mgNO2-N·L-1, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Developmental Biology 2021Quote: ... We used the following primary antibodies: rabbit antibody against Protein Kinase Cα diluted at 1:500 (Antibody Registry ID: AB_477345, Merck), rabbit antibody against Recoverin at 1:1000 (AB_2253622 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were resuspended in 10% FCS-PBS again and fixed in 1% paraformaldehyde (PFA) for 10 min at room temperature and reaction quenched with 2.5M glycine (Merck) on ice and centrifuged at 400g for 8min ...
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bioRxiv - Immunology 2022Quote: ... samples were re-loaded four times before washing with 1 mL of 2% acetonitrile (ACN) (#1000292500, Sigma-Aldrich, Merck) in 0.2% acetic acid ...
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bioRxiv - Microbiology 2022Quote: Nasal sIgA was obtained from 1 ml nasal swab eluates by centrifugation (1 minute, 10 000 xg) and supernatant was sterile-filtered through a 0.4 μm pore (Merck). We determined sIgA concentrations by ELISA (Human IgA ELISA kit ...
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bioRxiv - Microbiology 2022Quote: ... and protein expression was induced O/N with 0.5 mM isopropyl 1-thio ß-D-galactopyranoside (IPTG) (Merck Chemicals). The next day ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... The sections were incubated overnight at 4°C with the following primary antibodies: KRT8 (Merck Millipore, MABT329, 1:100), p-ERK (Cell Signaling Technology ...
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bioRxiv - Immunology 2022Quote: ... The pH of the solution was adjusted to pH 7.1-7.4 with 1 M NaOH (Merck KGaA, Darmstadt, Germany) and filtered with a silicone- and latex free syringe (#4050-X00V0 ...
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bioRxiv - Neuroscience 2022Quote: Fixed tissue was cut into 1 cm fragments and first impregnated during 3h in a 15%-sucrose solution (Merck) in 1X PBS ...
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bioRxiv - Physiology 2022Quote: ... monoclonal anti-α-actinin (Sarcomeric) antibody produced in mouse (1:1000, Sigma-Aldrich, Merck, clone EA-53, Cat# A7811), at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... were diluted 1:400 v/v in 0.5% low gelling temperature agarose in Ames’ medium at 37°C (A1420; Merck). A thin film was polymerized over a pure agarose meniscus in a Petri dish and covered with Ames’ medium ...
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bioRxiv - Molecular Biology 2022Quote: ... The Lipid II extract was assessed by spotting 1-2 μL on a HPTLC silica gel 60F254 plate (Merck). The TLC plate was developed in a mixture of chloroform:methanol:water:ammonia (88:48:10:1 ...
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bioRxiv - Neuroscience 2022Quote: ... the second step consisted of incubating overnight in PBS-Triton with rabbit anti-Aldh1a1 (1:500, HPA002123, Sigma Merck) and goat anti-calbindin (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: Cells were harvested and lysed in RIPA buffer (50 mM Tris/HCl pH 7.5, 150 mM NaCl, 1 % (v/v) Triton X-100 (Merck), 0.5 % (w/v ...
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bioRxiv - Plant Biology 2022Quote: ... the CDS of wild type (WT) PpGS1b.1 and PpGS1b.2 were subcloned into pET30a vector (Merck, Darmstadt, Germany) including a N-terminal 6xHis-tag by PCR ...
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bioRxiv - Neuroscience 2023Quote: ... For the example images for survival assay neurons were incubated with chicken polyclonal anti-MAP2 (1:2000; Merck Milipore) and secondary antibody Alexa Fluor 488 ...
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bioRxiv - Bioengineering 2024Quote: ... THP-1 cells were treated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA, Merck Life Science NV) for 24 hours in a T75 culture flask ...
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bioRxiv - Cell Biology 2023Quote: ... protein thioesterase inhibitors (10 µM palmostatin and 0.2 mM HDSF (1-hexadecanesulfonyl fluoride)) and 250 U/ml Benzonase Nuclease (Merck). After 30 min (4°C) ...
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bioRxiv - Biochemistry 2024Quote: ... Diener Electronic) followed by channel surface functionalization using 1% (v/v) trichloro(1H,1H,2H,2H-perfluorooctyl) silane (Merck) in HFE-7500™ (3M™ Novec™).
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bioRxiv - Cell Biology 2024Quote: ... medium—composed of 6.7 g Yeast Nitrogen Base without amino acids (Difco 291940) and 0.8 g complete supplement mixture drop-out (Formedium DCS0019) in 1 L—supplemented with 2% glucose (Merck). Cells from glycerol stocks or agar plates were inoculated into YPD and incubated at 30°C on an orbital shaker at 250 rpm (in an Innova 4230 incubator ...
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bioRxiv - Neuroscience 2024Quote: ... for 180 min prior to washing and development in Streptavidin-tertiary reagent (1:500 in 2% BSA and 0.1% Triton [TBS]) conjugated to either Cyanine 3 (Cy3) (Merck) or Alexa Fluor 488 (Life technologies ...
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bioRxiv - Developmental Biology 2024Quote: mESCs were plated at a final density of 1 × 104 cells/cm2 on fibronectin-coated (MM Merck Millipore, FC010) µ-Slide 8 Well Glass Bottom Chamber Slides (Ibidi ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed once with PBS and then incubated with RPMI containing 1 µg / ml propidium iodide (PI) (Merck) and nigericin (Merck ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining of puromycin was performed by incubation with the anti-puro monoclonal antibody (1:1000, Clone MABE343, Merck) for 60 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining of puromycin was performed by incubation with the anti-puro monoclonal antibody (1:1000, Clone MABE343, Merck) for 60 min at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... the membranes were incubated o/n at 4°C in 10 ml washing buffer (1% [w/v] milk powder in TBS-T) containing a 1:10,000 dilution of anti-pan-ADP-ribose binding reagent MABE1016 (Merck) at 4°C48 ...
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bioRxiv - Microbiology 2024Quote: ... Whole cell samples were then analysed by immunoblot using anti-puromycin antibody (1:2000, anti-mouse, clone 12D10, Merck). As a loading control a duplicate SDS-PAGE was performed ...
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bioRxiv - Biochemistry 2024Quote: ... ROS quenching was carried out by treating cells with 5 mM N-acetyl cysteine (NAC) (616-91-1, Merck) for 1 h.
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (10,000 x g, 30 min, RT) and incubated for 1 hour with Ni-NTA His bind resin (Merck) rolling at RT ...
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bioRxiv - Cell Biology 2023Quote: ... 25 mM Tris-HCl, 25 mM NaCl, 1 mM EDTA, pH 7.6, supplemented with 1X protease inhibitor cocktail i.e. PIC, Merck #4693132001). Further ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM K4Fe(CN)6 and 1 mg/ml X-gal (Roth)) and counterstained with nuclear fast red (Merck) for 5 min ...