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Citations for Merck :
2701 - 2750 of 3431 citations for Rat Interleukin 1 Receptor Antagonist IL1RN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... the cells were washed once with DPBS and stained with 1 μg/ml Hoechst 33342 (Merck, Zug, CH) in a Live Cell Imaging Solution (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... in Biotin binding buffer (5 mM Tris-HCl pH 7.5 [Sigma], 0.5 mM EDTA [AppliChem], 1 M NaCl [Merck]) for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.0) supplemented 5 mM benzamidine (Carl Roth), 0.1 mM AEBSF (AppliChem, Darmstadt, Germany) and Benzonase (1:1000, Merck) for 1 h at 4°C on a wheel rotator ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed and blocked as per western blotting protocol and probed with 1:1500 BG4 (Merck Millipore), 1:1500 DYKDDDK tag (anti-flag ...
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bioRxiv - Developmental Biology 2020Quote: ... The gels were subsequently demulsified with 45 µl 1H,1H,2H,2H Perfluoro 1 octanol (PFO) (Merck, #370533) into 200μl of MM+ Ri medium ...
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bioRxiv - Molecular Biology 2020Quote: ... the permeabilized cells were incubated for 1 hour with anti-phospho-S10 Histone H3 (06-570, Merck Millipore) in PBS containing 0.1% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... were used at a 1/10000 dilution before reaction with Immobilon Forte Western HRP substrate (WBLUF0100, Merck Millipore).
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bioRxiv - Bioengineering 2019Quote: ... Samples were post-fixed for 1h in a 1% osmium tetroxide solution in 0.1M sodium cacodylate buffer (Merck) and rinsed three times in the same buffer ...
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bioRxiv - Systems Biology 2020Quote: Agarose pads were made by pipetting 1 ml of 1.5 % (wt/vol) low melt agarose (Merck, Darmstadt, Germany) dissolved in PBS onto a 22 mm2 cover glass slide ...
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bioRxiv - Neuroscience 2019Quote: ... cells were fixed with 4 % paraformaldehyde and stained for doublecortin (guinea pig anti-DCX IgG, 1:400, Merck Millipore followed by Alexa 488 anti-mouse ...
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bioRxiv - Molecular Biology 2019Quote: ... and were hybridized with probes at a concentration of 1 µg/ml in DIG Easy Hyb (Merck, #11603558001). The hybridized probes were detected with anti-Dig AP (Merck ...
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bioRxiv - Evolutionary Biology 2019Quote: All experiments were carried out in casamino acids medium (CAA) containing 5 gl-1 casamino acids (Merck, Switzerland), 1.18 g l−1 K2HPO4*3H2O ...
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bioRxiv - Biochemistry 2020Quote: TSEN complexes were mixed to a final concentration of 1 or 3 μM with 4x SYPRO Orange (Merck) stock in 50 mM HEPES-NaOH ...
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bioRxiv - Microbiology 2021Quote: ... To ensure sterility 1/10 of the inactivated material was incubated in thioglycolate-medium (Merck KGaA, Darmstadt, Germany) for seven days without growth before material was taken out of the BSL3 laboratory.
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bioRxiv - Biochemistry 2020Quote: ... anti-RNA polymerase II subunit B1 (phospho CTD Ser-2) clone 3E10 (Merck, 04-1571-I, 1:5,000), and anti-RNA polymerase II CTD repeat YSPTSPS antibody clone 8WG16 (Abcam ...
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bioRxiv - Bioengineering 2021Quote: INS-1 cells were stained with red fluorescent membrane-inserting dye PKH-26 (Lot# SLBW0232, Merck, Darmstadt, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and mouse monoclonal anti-APP (1:500, 22C11; Cat#Mab348, RRID: AB_94882, Merck Millipore/Sigma, Burlington, MA, USA) antibodies were purchased from the indicated suppliers ...
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bioRxiv - Neuroscience 2021Quote: ... brain slices of mice were also incubated with a mouse NeuN (1:500, conjugated with AF488 MAB377X, Merck) and a rabbit GFAP antibody (1:1000 ab7260 ...
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bioRxiv - Plant Biology 2021Quote: ... dried and placed in a ten by ten grid pattern on square culture dishes containing 45 ml of nitrogen replete (+N) (0.5x Murashige and Skoog salts [Caisson Labs], 1% sucrose, 0.5 g/L MES and 0.4% Phytagel [Merck Sigma-Aldrich] ...
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bioRxiv - Systems Biology 2021Quote: ... protease inhibitor EDTA-free (cOmplete™, 1 tablet per 50 mL, cat no. 11873580001, Merck KGaA, Darmstadt, Germany), 1 mM MgCl2 ...
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bioRxiv - Microbiology 2020Quote: ... The following secondary antibodies were used: sheep α-rabbit horseradish peroxidase (HRP) (catalogue number AP510P, 1/10,000; Merck) and goat α-mouse HRP (STAR117P ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EGTA and protease inhibitors) on an Amicon 30 kDa cut off concentrator (Merck, Cat# UFC 503096). Protein concentration was determined using a BCA protein assay (Thermo Scientific ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM EDTA pH 7.2 with protease inhibitor tablets (cOmplete™, EDTA-free protease inhibitor cocktail, Merck, UK)) and sonicated 30 s on and 30 s off for three rounds using a XL-2020 sonicator (Heat Systems ...
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bioRxiv - Cell Biology 2020Quote: ... Proteasome complexes were subsequently blotted for 1 h at 200 V on ice onto PVDF membranes (Merck Millipore) and further handled as described above.
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bioRxiv - Biochemistry 2022Quote: ... the coding sequence was cut out from the pFastBac 1 construct and transferred to pET28a (Merck, Darmstadt, Germany) by means of HindIII and Eco53kI ...
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bioRxiv - Molecular Biology 2022Quote: AKT1/2 phosphorylation was inhibited with InSolution™ Akt Inhibitor VIII (1-10 µM, Merck KGaA, Darmstadt, Germany) in FCS-free medium for 3 hours prior to treatment with HGF (10 ng/µl).
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bioRxiv - Cell Biology 2022Quote: ... For cell cycle distribution the fixed cells were resuspended in Hoechst 33258 (Merck, B2883 1 µg/ml H2O). For nocodazole treatment ...
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bioRxiv - Cell Biology 2022Quote: ... The cells at 13 days and 20 days after electroporation were treated with doxycycline (1 µg/mL, Merck) for 24 hours and subjected to microscopic and flow cytometric analyses ...
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bioRxiv - Biophysics 2022Quote: Purified FHL was concentrated to 1-2 mg/mL using an Amicon centrifugal concentrator (100-kDa cutoff; Merck) and 3 µL of protein solution was applied to freshly glow-discharged C-flat 1.2/1.3 or 2/1 holey carbon grids (Protochips) ...
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bioRxiv - Genetics 2021Quote: YA synchronized wild type and unc-1(vlt10) animals were collected in RIPA 1X buffer (Sigma-Aldrich-Merck) to obtain protein extract ...
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bioRxiv - Neuroscience 2020Quote: ... a short protein sequence expressed in the injected virus (1:400 rabbit anti P2A; ABS31, Merck, Darmstadt, Germany). In the third step ...
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bioRxiv - Biochemistry 2020Quote: ... the water used was type 1 water purified by a Milli-Q® water purification system from Merck Millipore (Billerica ...
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bioRxiv - Neuroscience 2020Quote: ... neuronal somata were visualized by NeuN immunolabeling (1:1000, monoclonal mouse anti-NeuN IgG, #MAB377, /Merck, Darmstadt, Germany) in CRH-ires-cre ...
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bioRxiv - Cancer Biology 2020Quote: Cells were washed twice with cold PBS and lysed by addition of Tris-buffered saline (TBS)-lysis buffer (TBS [50 mM Tris-HCl pH 7.5, 150 mM NaCl], 0.5% Nonidet P-40, 1× protease inhibitor cocktail [Merck] ...
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bioRxiv - Immunology 2020Quote: ... Protein was concentrated to 1 ml using a Amicon Ultra-4 Ultracel 30 kDa spin concentrator (Merck Millipore) and the protein was separated on a HiPrep 16/60 Sephacryl S-200 HR column (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde for 30 min and stained with 1% crystal violet (Merck, Darmstadt, Germany) for 20 min at RT ...
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bioRxiv - Neuroscience 2019Quote: ... cells were fixed using 4% paraformaldehyde and stained with antibodies against ZO-1 (rabbit polyclonal, #617300, Merck-Millipore) and Nestin (mouse monoclonal ...
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bioRxiv - Neuroscience 2019Quote: ... samples were mixed with chilled buffer (50 mM Tris-HCl pH 9, 1% DOC, 50 mM NaF [Merck-Millipore] ...
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bioRxiv - Physiology 2020Quote: ... following which they were incubated with anti-MAP-2 antibody (AB5543, 1:500, Merck Millipore, Burlington, MA, USA) in blocking buffer overnight at 4℃ ...
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bioRxiv - Biochemistry 2021Quote: ... Deionized water was purified using an in-house Synergy Type 1 Ultrapure Water System from Milli-QR (Merck). All solvents were HPLC graded (Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Methyl viologen dichloride hydrate (paraquat, 98% purity) and Isopropil-β-D-1-tiogalactopiranósido (IPTG) were purchased from Merck.
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bioRxiv - Biochemistry 2021Quote: ... Peak fractions were pooled and concentrated to 10 mg.mL-1 using a 10,000 MWCO centrifugal concentrator (Merck Millipore). Protein was flash-frozen in liquid nitrogen and kept at −80°C until required ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated overnight at 4°C with monoclonal anti-NeuN antibody (1:500; Mouse, MAB377, Merck Millipore), in a 50% dilution of blocking buffer + 0.5% Triton X-100 ...
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bioRxiv - Developmental Biology 2021Quote: ... based on previously published sequences (Wu et al., 2018) (Supplementary Table 1) and co-injected alongside tracrRNA (Merck) in equimolar ratios and EnGen®Spy Cas9 NLS protein (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... 1% Triton X-100) and 20μg was immunoprecipitated overnight at 4°C with 10μg S9.6 antibody (Merck, MABE1095) conjugated to 100μL Pierce ChIP-grade protein-A/G magnetic beads (Thermo ...
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bioRxiv - Cell Biology 2021Quote: Human hTERT RPE-1 cells (ATCC, CRL-4000) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM/F12, Merck) supplemented with 10% fetal calf serum (FCS) ...
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bioRxiv - Molecular Biology 2019Quote: ... and then incubated with anti-γ-H2AX antibody (05-636, Merck, diluted at 1:500 in BD Buffer) for 2 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 150 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 1:100 diluted EDTA-free protease inhibitor cocktail [Merck]) for 45 min before clarification (21,000×g ...
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bioRxiv - Bioengineering 2022Quote: ... The space between the tip wall and the tubing was filled with 20 μL HFE-7500 containing 0.1% 008-Fluorosurfactant (RAN Biotechnologies) and 35% 1-bromo-3,5-bis(trifluoromethyl)benzene (Merck). Each PTFE tubing was threaded four times through the fluidic connector and fused to wider PE tubing (0.38 mm ID ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were stained with 1 mL 10% Giemsa’s azur eosin methylene blue solution (Merck KgaA, Darmstadt, Germany) in milli-Q water for 10 min ...