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Citations for Merck :
2751 - 2800 of 3062 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... they were co-cultured with HB-82 cells in absence or presence of 5 ng/mL tacrolimus (Merck) in an E:T ratio of 1:1 for 48 h ...
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bioRxiv - Cell Biology 2023Quote: Glass bottom well plates (Celvis) were coated with 5 µg/ml of human plasma fibronectin purified protein (Merck) for one hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... were concentrated to 5 mL by 10 kDa Amicon® Ultra Centrifugal Filters (Lot No. R9EA18187, Merck Millipore) and then applied for CsCl density gradient centrifugation at 24,000 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing the target protein were concentrated to 5 mL with Amicon Ultra-15 10 kDa (Merck). The concentrated sample was loaded onto a 320 mL of HiLoad Superdex 75 (GE Healthcare Life Science ...
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bioRxiv - Neuroscience 2023Quote: ... in the positive mode and SeQuant ZIC-pHILIC column (150 mm × 2.1 mm, 5 μm, Merck Millipore, 150460) in the negative mode ...
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bioRxiv - Molecular Biology 2024Quote: ... The bound complexes were eluted in lysis buffer 2 containing 25 mM glutathione or 5 mM biotin (Merck), respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... For liquid chromatography a SeQuant ZIC-pHILIC 5 μm Polymeric column 150 * 2.1 mm (Merck Millipore, Darmstadt, Germany) was used ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the supernatant was collected and aliquots of 5 µL were spotted onto silica gel 60 plates (Merck Millipore) and dried prior to chromatography ...
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bioRxiv - Molecular Biology 2024Quote: 300 µL 0.5% digest buffer (10 mM Tris-HCl pH 7.5, 10 mM EDTA pH 8.5, 50 mM NaCl (Merck) and 0.5% sodium dodecyl sulfate ...
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bioRxiv - Cancer Biology 2024Quote: ... T865.3) was followed by 5 minutes of rinsing with water and 20-30 seconds of Eosin solution (Merck, 115935 ...
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bioRxiv - Biochemistry 2024Quote: ... 5 µg/mL leupeptin) supplemented with 250 µg/mL lysozyme (from hen egg white; Merck KGaA, Darmstadt, Germany) and incubated on ice for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... centrifuged 5 min at 2000rpm and concentrated 10 times using Amicon® Ultra 15 mL Filters (Merck #UFC910096). The concentrated virus aliquoted and stored at -80°C.
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bioRxiv - Microbiology 2023Quote: ... separation was performed using a SeQuant zic-pHILIC column (150 mm × 4.6 mm, 5 µm particle size; Merck) at 25°C ...
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bioRxiv - Biochemistry 2023Quote: ... The enzyme solution was exchanged with 5 mM sodium acetate buffer (pH 5.0) using Amicon Ultra 10,000 molecular weight cut-off (Merck). The absorbance of the sample at 280 nm was measured using a spectrophotometer V-650 (Jasco ...
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bioRxiv - Microbiology 2023Quote: ... mixed with ∼5 x 105 Jiyoye cells in the presence of 8 µg/ml polybrene (Sigma-Aldrich/Merck) and spun at 800 g for 2 h ...
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bioRxiv - Microbiology 2023Quote: ... and stained with 5 μl each of FITC labelled annexin V and propidium iodide (PI; Sigma-Aldrich/Merck) for 5 minutes at room temperature in the dark ...
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bioRxiv - Biophysics 2022Quote: ... The PVDF membrane was blocked in 5 w/v% milk (skim milk powder for microbiology, Millpore, Merck KGaA) in PBS for 1 hour on an orbital shaker (SC5 ...
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bioRxiv - Cancer Biology 2022Quote: ... The ChIP experiments were performed using the following antibodies: CTCF (07-729, Merck Millipore, 5 ml per ChIP), RAD21 (ab154769 ...
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bioRxiv - Cell Biology 2022Quote: ... Metabolite samples (2.5 μL) were separated using a ZIC-pHILIC stationary phase (2.1 × 150 mm, 5 μm) (Merck). The autosampler temperature was 4 °C and the column compartment was maintained at 25 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Following saturation for 1 h in PBS 1X containing 5% milk and 0.1% Tween 20 (655204-100ML, Merck), the membranes were incubated overnight at 4 °C with primary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... cryo-sections were permeabilized and blocked with blocking solution (5% BSA containing 0.3% Triton X-100 (Merck, 93420)) for 1-2 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The samples were cleaned twice in Xylene for 5’ and mounted with DPX mounting medium (Merck, Overijse, Belgium).
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bioRxiv - Cell Biology 2024Quote: ... then washed 5 times in PBS and permeabilized in 0.5% Triton X-100 (Cat. No. X100, Merck, Germany)-TBS for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... which was treated with fresh bisulfite solution (2.5 M sodium metabisulfite, Merck; 125 mM hydroquinone, Sigma; pH 5) for 4h ...
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bioRxiv - Molecular Biology 2024Quote: ... then cells were made electrocompetent via 5 washing steps using 10% glycerol in ultrapure water (Milli-Q, Merck), and resuspended in 40 µl of 10% glycerol in ultrapure water ...
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bioRxiv - Biophysics 2024Quote: ... The fractions containing the target protein were concentrated to 5 ml with Amicon Ultra-15 10 kDa (Merck). The concentrated sample was incubated with uTEV3 protease25(Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... were injected and separated using a ZIC-pHILIC guard-analytical column (SeQuant; 20 mm × 2.1 mm; SeQuant; 150 mm × 2.1 mm, 5 μm; Merck) on an Ultimate 3000 HPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... Individual mosquito’s homogenates were filtered using 0·5 mL PVDF ST ultra-free-cl millipore (Merck, Darmstadt, Germany) and diluted (1/8 ...
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bioRxiv - Neuroscience 2024Quote: ... Compounds were diluted in a vehicle consisting of sterile 0.9 % physiological saline solution containing 5 % DMSO (Merck Millipore) and 5 % Kolliphor EL to achieve a final concentration of 30 μM ...
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bioRxiv - Immunology 2024Quote: The HPLC setup consisted of a ZIC-pHILIC column (SeQuant; 150 mm x 2.1 mm, 5 µm; Merck), with a ZIC-pHILIC guard column (SeQuant ...
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bioRxiv - Molecular Biology 2024Quote: ... Separation was performed on a SeQuant ZIC-HILIC column (150 × 2.1 mm, 5 µm; Merck KGaA, Darmstadt, Germany). The mobile phase consisted of 20% methanol with 15 mmol/L ammonium formate and 80% acetonitrile ...
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bioRxiv - Molecular Biology 2024Quote: ... The ChIP experiment was performed with the following antibody: CTCF (07-729, Merck Millipore; 5 µL per ChIP). The purified DNA fragments were prepared according to the protocol of the KAPA LTP Library Preparation kit (KR0453 ...
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bioRxiv - Cell Biology 2024Quote: ... washed 2x with cold PBS and scraped using 1 ml of cold lysis buffer (50 mM Tris HCl, pH 7.4,150 mM NaCl, 1% Triton X-100, 5 mM CaCl2 (Merck #1023821000 ...
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bioRxiv - Cancer Biology 2024Quote: Pharmacological inhibitors and chemical compounds were used at the following concentrations: 100 μM Cholesterol (Merck, #57-88-5), 100 μM MβCD (lipid raft inhibitor ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 min) and resuspended at 1.0×106 cells per mL in IMDM supplemented with 0.1% delipidated BSA (Merck) and 10 mM HEPES ...
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bioRxiv - Systems Biology 2021Quote: ... and kept at -20 °C. Chlorpromazine (in house synthesized based on (Galons et al. 1985)) and loperamide HCl (Sigma-Aldrich, Merck KGaA, Germany) were freshly dissolved in water and filtered prior to the treatment ...
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bioRxiv - Microbiology 2020Quote: ... the samples were pre-enriched overnight in buffered peptone water (Oxoid, Basingstoke, UK) at 37°C and then enriched in tetrathionate brilliant green broth (Merck KGaG, Darmstadt, Germany) at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... These intestinal pieces were washed thoroughly with PBS and digested for 30 min at 37 °C with 0.5 mg/mL collagenase D (Merck/Sigma-Aldrich, cat#11088858001) in RPMI medium (Gibco ...
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bioRxiv - Genetics 2023Quote: ... and neo-resistance MEFs were established from WT Crl:CD1 (ICR) or ICRneo [27] foetuses at 14 d post-coitus and inactivated using 10 μg/mL mitomycin C (#M0503; Merck KGaA, Darmstadt, Germany). MEFs were cultured in the primary medium [Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Molecular Biology 2024Quote: ... The plate was washed three times with TBS-C blocking solution and incubated with goat-anti mouse IgG-HRP (A4416, Merck; dilution 1/10,000) added in TBS-C blocking solution and incubated during 1 h at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... then stored in sterilized polypropylene bottles (Nalgon, Cat. No. 2330) at 8° C until filtered using 0.22 µm Sterivex filters (Merck-Millipore, Cat. no. SVGP01050) in 60 mL increments using disposable syringes ...
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bioRxiv - Plant Biology 2024Quote: ... 20 mg of grounded wheat flour was extracted with 100 μL MilliQ Water and 450 μL of ice-cold (−20 °C) methanol: ethanol (1:1 v/v; LiChrosolv®, Merck, Darmstadt, Germany) spiked with 1 ppm Succinic Acid 13C2 (Cambridge Isotope Laboratories ...
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bioRxiv - Immunology 2021Quote: ... The plates were then washed 5 times with HSWB followed by incubation of goat anti-mouse antibody (Merck, Germany) conjugated with HRP (diluted 1:3000 in LSWB + 1% BSA ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Neuroscience 2020Quote: ... also in with PBS containing 2.5%, bovine serum albumin (BSA; A1391-0250; Applichem, Germany) and 0.1 % Tween20 (9005-64-5; Merck, Germany). For visualizing neuronal membranes ...
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bioRxiv - Neuroscience 2020Quote: ... and were then blocked with PBS containing 2.5%, bovine serum albumin (BSA) (A1391-0250; Applichem, Germany) and 0.1 % Tween20 (9005-64-5; Merck, Germany) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... neurons were permeabilized and blocked with PBS containing 2.5%, bovine serum albumin (BSA) (A1391-0250; Applichem, Germany) and 0.1 % Tween20 (9005-64-5; Merck, Germany) for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... Tumors and lungs were paraffin-embedded and consecutive 5-μm sections were stained with hematoxylin-eosin purchased from Merck.
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miR-206 inhibits estrogen-induced proliferation and invasion of ER-α36 positive gastric cancer cellsbioRxiv - Cancer Biology 2021Quote: ... The PVDF membrane was sealed with 5% skim milk powder in the PBS containing tween (Sigma-Aldrich; Merck KGaA) for 3 h ...