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Citations for Merck :
2551 - 2600 of 3062 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 50 ml centrifuge tubes containing 5 ml of Tryptic Soy Broth (TSB, Merck KGaA, Darmstadt, Germany) were inoculated with one colony from an overnight culture (see above ...
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bioRxiv - Cell Biology 2021Quote: ... The following small molecule inhibitors were used at the indicated concentration: nocodazole (5 µM; Merck Millipore), CK-666 (100 µM ...
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bioRxiv - Biophysics 2021Quote: ... were first pre-treated with 5 and 10 μM concentrations of montelukast sodium hydrate (PHR1603, Merck) or saquinavir mesylate (1609829 ...
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bioRxiv - Cancer Biology 2020Quote: ... media was replaced with media containing 10 nM 5-Bromo-2′-deoxyuridine (BrdU) (Merck, B5002-100MG). Coverslips were then fixed ...
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bioRxiv - Cell Biology 2021Quote: ... and 5 U ml−1 penicillin and 50 μg ml−1 streptomycin (Merck Life Science (Sigma)) at 37 °C and 5% CO2 ...
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bioRxiv - Biophysics 2020Quote: ... one cycle for 5 min) in Amicon Ultra 100 kDa MWCO filters (Merck Millipore, Darmstadt, Germany) using reaction buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... the medium was removed and cells were covered with 5 µm isopore membrane (cat #: TMTP04700, Merck) and irradiated with 100 J/m2 of UV-C ...
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bioRxiv - Genomics 2022Quote: ... The supernatant was filtered with a 5-ψm MF-Millipore membrane filter (Merck Millipore, Milan, Italy). The flow-through was centrifuged at 10,000 ×g for 5 min with a benchtop centrifuge (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... The filters were blocked with TBS containing 0.05% Tween and 5% non-fat dry milk (Merck). Incubation was done overnight with the indicated primary antibody dilutions ...
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bioRxiv - Systems Biology 2020Quote: ... and a ZIC-pHILIC guard column (20 mm x 2.1 mm x 5-micron packing, Merck) under alkaline mobile phase conditions with ESI ion source ...
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bioRxiv - Systems Biology 2022Quote: ... using a C18 and ZIC-pHILIC column (150 mm × 4.6 mm, 5 μm column, Merck Sequant). The column was maintained at 30°C and samples were eluted with a linear gradient (20 mM ammonium carbonate in water ...
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bioRxiv - Bioengineering 2022Quote: ... 5% CO2 in a humified incubator in Dulbecco’s Modified Eagle Medium (DMEM #D5671, Merck, Darmstadt, Germany) supplemented with 10% Fetal Calf Serum (FCS ...
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bioRxiv - Molecular Biology 2021Quote: ... washed 3x 5 minutes in ddH2O and cover-slipped with Entellan mounting medium (Merck, Darmstadt, Germany). Images were acquired on an Olympus BX61 microscope.
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bioRxiv - Microbiology 2021Quote: ... The samples were deproteinized by addition of 10 μL of cold 5-sulphosalicilic acid (SSA, Merck) (300 mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... before being incubated for 5 secondes in Luminata Classico Western HRP substrate (Merck Millipore, Molsheim, France) and then imaged for chemiluminescence using the ChemiDoc Touch Imaging System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... E15.5 or P7 CGNPs were plated as described above in the presence 5 μM Cyclopamine (Merck), 250 nM Purmorphamine (Stem Cell Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... washed four times with PBS and permeabilized for 5 min with 0,5% Triton X-100 (Merck) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Chromatographic separation was performed on a Sequant ZIC-pHILIC column (5 μm, 2.1 × 150 mm; Merck) maintained at 45°C ...
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bioRxiv - Microbiology 2022Quote: ... 50 and 70% for 5 min and 95% and 100% twice for 10 min (Merck Millipore). The coverslips were then critical-point-dried using an EM DPC 300 critical point drier (Leica ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 µm) coupled with a SeQuant® ZIC®-pHILIC Guard (20 mm × 2.1 mm) (Merck) was used for analyte separation ...
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bioRxiv - Neuroscience 2023Quote: ... 5 % Natural Goat Serum (Goat serum from controlled donor herd, G6767-100ML, Sigma Aldrich, Merck, Germany) and primary antibodies (see Table 3 ...
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bioRxiv - Microbiology 2023Quote: ... The samples were spotted on a 5 x 10 cm TLC silica gel 60 F254 (Merck) and developed in petroleum ether:diethyl ether (9:1) ...
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bioRxiv - Molecular Biology 2023Quote: ... reduced with 5 mM TCEP for 30 min and alkylated with 10 mM iodoacetamide (Merck, I1149) for 30 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 50 μL magnetic beads were incubated with 5 μg of Ago-1 antibody (Merck, Millipore, Germany) or IgG antibody (Merck ...
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bioRxiv - Cell Biology 2023Quote: Oocytes were cultured in G-IVF PLUS (Vitrolife, #10136) under mineral oil (Merck, # 8042-47-5) at 37°C in 5% O2 ...
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bioRxiv - Biophysics 2023Quote: ... adult male Wistar rats aged ∼ 5 weeks were given an intraperitoneal injection of crotaline (Merck, NJ) to induce pulmonary arterial hypertension ...
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bioRxiv - Cell Biology 2022Quote: ... a SeQuant ZIC-pHILIC (100 mm, 2.1 mm I.D. and 5 μm particle size, Merck, Germany) column was used ...
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bioRxiv - Cancer Biology 2022Quote: ... For final and irreversible sample dehydration a graded serie (25%, 50%, 75% and 100%, 5 min each) of 1,1,1,3,3,3-hexamethyldizilazane (HDMS, Merck, Millipore) was used ...
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bioRxiv - Cell Biology 2023Quote: ... Parasitemia was maintained below 5% and determined via Hemacolor staining of blood smears (Sigma-Aldrich, Merck) using a Nikon Eclipse E100 microscope (Nikon Corporation ...
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bioRxiv - Cell Biology 2023Quote: ... Preparations were thereafter fixed with 100% methanol for 15 s and stained with 5% Giemsa (Merck) for 15 minutes ...
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bioRxiv - Immunology 2023Quote: Resuspended HLA-I-eluted samples were centrifuged through 5 kDa cutoff filters (Merck Millipore #UFC3LCCNB-HMT) and vacuum-dried ...
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bioRxiv - Evolutionary Biology 2023Quote: ... For removal of chorion embryos were incubated for 5 mins with 1 mg/ml Pronase (Merck) then washed with culture medium and flash-frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were permeabilized and blocked with PBS containing 0.1% Triton X (#9005-64-5, Merck, Germany), 5% ...
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bioRxiv - Biochemistry 2024Quote: ... the spacer region between them and flanking regions of 5 bp were ordered from Merck (Germany). The 5’ end of sense strand of each oligonucleotide was labeled with Cyanine 5 dye (Cy5 ...
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bioRxiv - Molecular Biology 2024Quote: ... animals were transferred to 10 cm NGM agar plates containing 5-FU (10 µM) (Merck, #F6627) to prevent progeny production ...
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bioRxiv - Molecular Biology 2024Quote: ... animals were transferred to 10 cm NGM agar plates containing 5-FU (10 µM) (Merck, #F6627) to prevent progeny production ...
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bioRxiv - Genetics 2024Quote: ... Membranes were blocked for a minimum of 30 min in 5% Skim Milk Powder (Merck, #70166) in PBS + 0.1% Tween-20 (PBS-T ...
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bioRxiv - Immunology 2024Quote: ... slides were rinsed in double-distilled water and counterstained with Hoechst dye (5 μg/mL; Merck). Stained slides were subsequently imaged using an LSM700 confocal microscope (Zeiss).
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bioRxiv - Microbiology 2024Quote: ... the bacterial isolates were cultured overnight in 5 mL of sterile Muller-Hinton broth (Merck, Germany) at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... Coverslips were re-calibrated in PBST (PBS with 0,2% Tween 20 Merck, Cat 9005-64-5) for 5 minutes and 50ul of primary antibody (rabbit anti-BRD4 ...
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bioRxiv - Bioengineering 2024Quote: ... and 5 mg ml-1 fibrinogen from bovine plasma type I-S (Merck KGaA, Darmstadt, Germany); then ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by 5 min in a 1:1 mixture of propylene oxide (Fluka, Merck, Darmstadt, Germany) and SPURR (Low Viscosity Spurr Kit ...
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bioRxiv - Developmental Biology 2024Quote: ... Females were stimulated with 5 IU of pregnant mare serum gonadotropin (PMSG; Folligon; Merck Animal Health) per mouse 46 hours prior to oocyte isolation ...
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bioRxiv - Microbiology 2020Quote: ... After 2 hours at 30°C with shaking 1 ml of cells was pelleted and soluble proteins were extracted using BugBuster (Merck). For internalisation experiments involving plasmid-complemented deletion strains ...
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bioRxiv - Molecular Biology 2020Quote: ... Three individual clones were picked and amplified in LB broth including antibiotics for 12 h at 37°C and isolated using GenElute– HP Plasmid MaxiPrep Kit (Merck). Integrity of purified expression constructs was validated by gel electrophoresis and the concentration was determined by absorbance measurement ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked with 10% bovine serum albumin (BSA) 30 min at 37°C and incubated with primary anti-γH2A.X mouse antibodies (Merck Millipore) or rabbit anti-LC3B antibodies (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2021Quote: ... samples were heated 5 minutes at 95°C and 30µl were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
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bioRxiv - Cell Biology 2021Quote: ... cut axially using a size 10 surgical scalpel into 1mm3 pieces and incubated at 37°C for 45 mins in Liberase (Merck) and 10ul/ml DNAse I (Thermo Scientific) ...
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bioRxiv - Systems Biology 2020Quote: ... The reaction was incubated at 25°C for 90 minutes followed by addition of 1 μL proteinase K (Merck, 3115887001) and incubation at 37°C for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed in TBS and demi-water before being dried on a heat plate at 37°C before and cleared in xylene and mounted with Entellan (Merck).