Labshake search
Citations for Merck :
2551 - 2600 of 4715 citations for Tumor Protein Translationally Controlled 1 TPT1 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... and proteins in the lysate were separated in a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). The membrane was incubated with 5 % skim milk (BD Biosciences ...
-
bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS- PAGE and those containing pure tag free CBX8 protein were pooled and concentrated using Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Protein was exchanged into a buffer consisting of 20mM MES pH 6.5 ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 10,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL).
-
bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Protein was exchanged into a buffer containing 25 mM Tris ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions containing the desired protein were pooled and concentrated to 2 ml in Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Concentrated protein was loaded onto a HiLoad 26/60 Superdex 75 prep grade column (GE Healthcare ...
-
bioRxiv - Immunology 2020Quote: Following protein digestion a 5% volume of the peptide solution was cleaned up with a reverse phase (C18) ZipTip (Merck Millipore, Burlington, MA, USA). The peptide sample was acidified with the addition of 1 μl 1M hydrochloric acid ...
-
bioRxiv - Immunology 2022Quote: ... Tissue samples for RNA-protein extraction were cut into small pieces (< 5 mm) and placed in a microcentrifuge tube containing RNAlater® solution (Merck Ltd, Dorset, UK) and stored at −70°C until further use.
-
bioRxiv - Microbiology 2023Quote: Proteins in the lysate were separated on a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). The membrane was incubated with 5 % skim milk (BD Biosciences ...
-
bioRxiv - Microbiology 2024Quote: ... 15 µg of total protein in a volume of 47 µl 1x sample buffer was treated with 7 U of benzonase (#70746; Merck Millipore, Burlington, MA, USA) in dilution buffer (20 mM HEPES pH 8.0 ...
-
bioRxiv - Immunology 2024Quote: ... Equal amounts of protein were then separated through 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore Ltd., Tullagreen, Ireland). Membranes were blocked in 5% nonfat dry milk in 1× TBST for 1 hour at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... the protein was concentrated to the desired concentration using centrifugal filters (#UFC800308; Amicon® Ultra centrifugal filters, Ultracel® −3K or larger; Merck Millipore Ltd.)
-
bioRxiv - Cell Biology 2023Quote: ... Gels were run in 1x running buffer and then the proteins were transferred from the gel in ice cold 1x Buffer onto Immobilon®-P Transfer membranes (Merck Millipore, Cat.No. IPVH00005). Transfer membranes were air dried to dehydrate and stored until use ...
-
bioRxiv - Cancer Biology 2019Quote: ... GST was labeled with mouse Anti-GST Tag monoclonal antibody (05-782, Merck Chemicals; 2.5 μg/ml). To account for variations in the amount of fusion protein ...
-
bioRxiv - Cancer Biology 2019Quote: ... The membrane was incubated with a primary antibody that recognized Pt-induced DNA adducts (ICR4, Merck, Millipore), then washed with TBST and incubated with an HRP-conjugated secondary antibody according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: Cell surface receptor expression was analyzed in all cell lines with the following antibodies: cetuximab (EGFR) (Merck), trastuzumab (HER2 ...
-
bioRxiv - Neuroscience 2022Quote: ... and one of the following primary antibodies: mouse anti-GAD67 for staining GABAergic cells (Merck & Co., MAB5406), goat anti-Substance P for staining D1-containing cells (Santa Cruz ...
-
bioRxiv - Immunology 2022Quote: ... IL-6 and IL-1RA was performed using the Luminex color-coded antibody-immobilized beads from Merck Millipore following the manufacture’s indications ...
-
bioRxiv - Neuroscience 2020Quote: ... glass slides were incubated with an anti-DIG-AP antibody (Anti-Digoxigenin-AP, Fab fragments; Merck, Germany) overnight in a humidified chamber ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... were captured on the same chip by a previously immobilized anti-Fc antibody (Merck Life Science S.r.l). Anti-FLAG or anti-Fc antibodies were immobilized by classical amine coupling chemistry (29 ...
-
bioRxiv - Immunology 2019Quote: ... 5 μg of the following antibodies were used: H4ac (Histone 4 pan-acetyl, Merck Millipore #06-866) anti-H3K27ac (Abcam ab4729) ...
-
bioRxiv - Immunology 2020Quote: ... and the supernatant was taken for immunoprecipitation with 2 μg of anti-ubiquitin antibody (FK2, Merck-Millipore) for 12 hours at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... H4S1p was detected using a rabbit anti-phospho H4/H2A S1p antibody (07-179; Upstate/Merck-Millipore) at a dilution of 1:4000 ...
-
bioRxiv - Genetics 2020Quote: ... membrane was divided into two halves and probed with either monoclonal anti-(c-Myc) antibody (Merck Millipore) or anti-α-tubulin antibody (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used for immunofluorescence in Drosophila: mouse anti-puromycin (clone 12D10, Merck Millipore) at 1:200 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The blotted membrane was incubated overnight at 4 °C with a polyclonal anti-ADAR antibody HPA051519 (Merck) diluted 1:500 with 0.1% TBST containing 5% BSA at final concentration ...
-
bioRxiv - Genomics 2023Quote: ... Primary antibodies were applied overnight at 4°C and included TH (Pel-Freez Biologicals #P40101 and Merck Millipore #AB9702 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Antibody cocktail was prepared in excess and filtered through a 0.1 µm centrifugal filter column (Merck Millipore) to remove antibody aggregates.
-
bioRxiv - Plant Biology 2023Quote: ... was secondarily reacted with the anti-LPOR antibody and detected using a chemiluminescence reagent (Immobilon; Merck Millipore) and an imager (LuminoGraph I ...
-
bioRxiv - Biochemistry 2023Quote: ... Antibodies usedwere: mouse monoclonal (BIII-136) anti human Band 3 (B9277, Merck Life Science S.r.l., Milan, Italy). Mouse monoclonal (H68.4 ...
-
bioRxiv - Biophysics 2024Quote: ... secondary antibodies goat anti-rabbit IgG and goat anti-mouse IgG conjugated with Atto 594 (Merck; 77671) and Abberior® STAR RED (Merck ...
-
bioRxiv - Biochemistry 2024Quote: ... and an alkaline phosphatase-conjugated goat anti-rabbit immunoglobulin G secondary antibody (Sigma-Aldrich, Merck, Barcelona, Spain), both at 1:1000 dilution.
-
bioRxiv - Immunology 2024Quote: ... we used anti-human TIM-3 IgG4 antibody (5 μg/ml, Merck & Co., Inc., Rahway, NJ, USA). Finally ...
-
bioRxiv - Immunology 2024Quote: ... The reduced antibody was washed in C buffer in 50 kDa Amicon® spin filters (Merck Millipore) before being mixed with the preloaded X8 polymer and incubated for 60-90 min at 37°C ...
-
bioRxiv - Biochemistry 2021Quote: ... The protein complexes were concentrated to 0.7-1 mg ml-1 using an Amicon Ultra-0.5 mL concentrating device (Merck) with a 100 kDa filter cut-off ...
-
bioRxiv - Genetics 2019Quote: ... These were then re-solubilized in 30μl chloroform/methanol (1:1) and loaded onto silica gel plates (Merck). PA and DAG were separated by 10-15min of thin layer chromatography (TLC ...
-
bioRxiv - Biochemistry 2020Quote: ... 1 mM EDTA and 1 mM DTT and concentrated with Amicon Ultra-0.5 mL centrifugal filter (Merck #UFC503096). Nucleosomes were stored in 4 °C and the quality of nucleosomes was assessed by 5-6 % TBE gel.
-
bioRxiv - Molecular Biology 2021Quote: ... in a 1:1 dilution with tris-buffered saline (TBS) containing 0.1% (v/v) Tween® 20 (Merck). Subsequently ...
-
bioRxiv - Biophysics 2022Quote: ... All pH adjustments were performed using 1 M HCl or 1 M NaOH (99.0 %, Merck KGaA, Darmstadt, Germany). All samples were filtered prior to stress application through a 0.1 µm inorganic membrane filter (#6809-2012 ...
-
bioRxiv - Immunology 2020Quote: ... rAAVDJ or rAAV6 genome plasmid and Donor plasmid at a 3:1:1 ratio in Polyethylenimine (PEI)(Merck). In total each plate was teransfected with 41,250ng of DNA ...
-
bioRxiv - Microbiology 2022Quote: ... the samples were mixed vigorously at a ratio of 1:1 with saturated phenol solution pH 4.3 (Merck) and centrifuged for 15 min at 13’000 rpm ...
-
bioRxiv - Plant Biology 2019Quote: ... 0.8 % phytoagar (Duchefa) and 10 µg mL-1 Glufosinate-ammonium or 25 µg mL-1 hygromycin B (Merck) for herbicide selection ...
-
bioRxiv - Plant Biology 2022Quote: ... 10 mM imidazole and 1 mM TCEP) supplemented with protease inhibitor cocktail EDTA-free (Merck Millipore; 1:200). Cells were stored at −80°C until use ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 × 107 wild-type U2OS or AsiSI-ER U2OS cells were crosslinked with 1% formaldehyde (Merck, 252549-100ML) for 10 min at 37°C and inactivated by the addition of glycine (Merck ...
-
bioRxiv - Neuroscience 2023Quote: ... supplemented with protease inhibitor cocktail set III (Cat No. 539134-1 set, 1:100, Merck Millipore, Rahway, NJ). The lysate was centrifuged at 16,000 g at 4 LJ for 10 minutes to remove insoluble samples ...
-
bioRxiv - Cell Biology 2023Quote: ... or propranolol hydrochloride (20 mg·kg-1 for the first five days and 10 mg·kg-1 from day six until euthanasia, Merck), diluted in 0.9% saline ...
-
bioRxiv - Neuroscience 2023Quote: ... HEK293T cells were lysed in lysis buffer (50 mM Tris HCl pH 7.4, 150 mM NaCl, 0.5% Triton X, 1 x cOmplete protease inhibitors [1x = 1 tablet in 40mL; Merck]), sonicated ...
-
bioRxiv - Molecular Biology 2023Quote: ... exponentially growing RPE-1 cells were pulse-labeled with 1 μM CldU (5-Chloro-2’-deoxyuridine, Merck, C6891) and 250 μM IdU (5-Iodo-2’-deoxyuridine ...
-
bioRxiv - Plant Biology 2024Quote: ... 1% acetic acid in H2O) containing an internal standard of 1 µM salicylic acid-d4 (SA-d4, Merck) was added ...