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Citations for Merck :
2801 - 2850 of 4715 citations for Tumor Protein Translationally Controlled 1 TPT1 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: MCF10 DCIS.com (DCIS.com) lifeact-RFP cells (Riedl et al., 2008) were cultured in a 1:1 mix of DMEM (Merck) and F12 (Merck ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then lysed in PK lysis buffer (50 mM Tris-HCl, pH 7.5, 1 mM EDTA, 150 mM NaCl, 1% Igepal-630 (Merck)) supplemented with 50 mM DTT and protease inhibitors (cOmplete™ protease inhibitor cocktail EDTA-free ...
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bioRxiv - Molecular Biology 2022Quote: ... Anti-K48-Specific Ubiquitin (05-1307, 1:1000) and anti-K63-Specific Ubiquitin (05-1313, 1:1000) from Merck-Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... Same volumes of the solvent phase were evaporated under nitrogen and lipids were resuspended in 100 µl of chloroform/methanol 1:1 (v:v) (Merck). Lipid extracts corresponding to 1 mg wet liver weight were applied onto HPTLC silica gel 60 plates (10 × 20 cm ...
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bioRxiv - Cell Biology 2021Quote: ... cultured in SF-900 II SFM at 27°C were transfected at a density of 0.4 × 106 ml-1 with 1 μg bacmid DNA using Excort IV Transfection Reagent (Merck) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... 10 mm NaF, 1 mm DTT, 0.1 mm Na3VO4, 1% Triton X-100, 0.1% SDS, and protease inhibitors; Merck, Millipore) and homogenized by sonification ...
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bioRxiv - Microbiology 2022Quote: ... To each tube 1 μL of Tris((1-benzyl-4-triazolyl)methyl)amine (TBTA) solution (2.5 mM in DMSO; Merck), 10 μL of Tetrakis(acetonitrile)copper(I ...
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bioRxiv - Neuroscience 2024Quote: ... and then blocked for 1 h at RT with 0.2% Triton-X in PBS + 1% normal goat serum (NGS, Merck). Antibodies used and their concentration is found in Supplementary Table 4 ...
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bioRxiv - Microbiology 2023Quote: ... 108 – 109 PFU (plaque-forming units) mL-1) in TSB (tryptone soya broth, Oxoid) or quarter-strength (1/4) Ringer’s buffer (Merck)) was used in this study ...
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bioRxiv - Microbiology 2023Quote: ... the lipids were resuspended in methanol:chloroform (1:1) and subjected to thin layer chromatography (TLC) using silica gel 60 plates (Merck). Total lipids were separated using chloroform:methanol:water (65:25:4 ...
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bioRxiv - Genomics 2023Quote: ... Fungal biomass was then digested by adding 100 µL of 1 mg mL-1 Chitinase from Streptomyces griseus (Merck) and 200 µL of 50 mM EDTA and incubating at 37 °C for 3 hours ...
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bioRxiv - Systems Biology 2023Quote: ... cells were washed twice with PBS and then cultured in a starvation medium composed of DMEM:F12 (1:1) supplemented with BSA (0.3% Sigma-Aldrich/Merck), L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... containing solid (7 g L−1 agar) full-strength Hoagland (2.75 mL well−1; pH adjusted to 6.5) (Sigma-Aldrich, Merck), sealed with parafilm and placed under the growth chamber ...
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bioRxiv - Biophysics 2024Quote: ... Total DNA was extracted by adding 1 mL of a 25:24:1 phenol:chloroform:isoamyl alcohol mixture (Merck, Darmstadt, Germany) along with 1 g of acid washed glass beads (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: A digestion buffer comprising of 0.05 mg/mL activated DNAse I (DNase I with HBSS in a 1:1 ratio, Merck), 10 mg/mL Collagenase (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... pLKO.1-puro-shRNACDK12 (TRCN0000196423 and TRCN0000368335) and pLKO.1-puro-shRNABRCA1 (TRCN0000244984 and TRCN0000244987) were purchased from Merck.
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bioRxiv - Genomics 2020Quote: ... ChIP was performed on native material (without crosslinking) using antibodies targeting histone modifications H3K4me2 (Merck ref. 07-030), H3K9me3 (Active Motif ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 μg of chromatin was immuno-precipitated with either 5 μg of CTCF antibody (07-729, Merck Millipore) or 4 μg of H3K27me3 antibody (17-622 ...
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bioRxiv - Bioengineering 2021Quote: ... Cetuximab antibody (Erbitux infusion solution, 5 mg/mL) was used to target EGFR and was purchased from Merck Serono ...
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bioRxiv - Cell Biology 2020Quote: ... An anti-DIG antibody conjugated to alkaline phosphatase and BM purple alkaline phosphatase substrate precipitating solution (Merck Roche) were used for staining ...
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bioRxiv - Molecular Biology 2022Quote: CobB driven deacetylation of His-PrsAc was analyzed by Western blot with anti-acetyl lysine antibodies (Sigma/Merck) and mass spectrometry ...
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bioRxiv - Biochemistry 2021Quote: ... were incubated overnight at 4°C with rotation with 20 µg/ml anti-FLAG M2 antibody (Merck, F3165) and 50 µl/ml of pre-washed Pierce magnetic protein A/G beads (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2019Quote: ... The cells were incubated for one hour at room temperature with a mouse monoclonal antibody against γH2AX (Merck) diluted in PBS/SVF (1/1000 ...
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bioRxiv - Cell Biology 2021Quote: ... This was followed by secondary antibody staining for 20 minutes before mounting onto glass slides using FluorSave (Merck)) ...
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bioRxiv - Neuroscience 2019Quote: ... The membranes were also stained with a mouse anti-actin antibody for 1h at room temperature (#MAB1501R, Merck).
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then incubated with 0.5 μg/ml of mouse anti-phosphotyrosine monoclonal antibody 4G10 (Merck, Darmstadt, Germany) at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... The ChIP experiments were performed using the following antibodies: CTCF (07-729, Merck Millipore, 5 ml per ChIP), RAD21 (ab154769 ...
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bioRxiv - Immunology 2024Quote: ... anti-DNP IgE sensitized BMMCs were pre-incubated with inhibitors or blocking antibody: 50 µM Y-27632 (Merck), 50 µM blebbistatin (Merck) ...
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bioRxiv - Molecular Biology 2022Quote: ... Immunodetection and FISH were conducted as described previously 78 using the following antibodies: rabbit H3K27me3 (#07-449 - Merck) diluted 1:200 ...
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bioRxiv - Microbiology 2023Quote: ... on magnetic rack and then resuspended in 300µL of ChIP buffer and 3µg of anti-Flag antibody (F1804-200UG, Merck) before being placed on wheel for 1h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with a biotinylated donkey antibody to rabbit IgG (10 μg/ml; AP182B, Merck Millipore) and then with Alexa488-conjugated streptavidin (2.5 μg/ml ...
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bioRxiv - Biophysics 2024Quote: ... The phosphorylation levels of PDGFRβ or CSF1R were detected by western-blotting using 4G10 antibody (Merck Millipore, Germany).
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bioRxiv - Biophysics 2024Quote: ... We incubated 500 μl CHAPS lysate with agarose beads conjugated with anti-Flag antibodies (Merck, Cat. No.: A2220) over night at 4 °C on an overhead shaker ...
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bioRxiv - Plant Biology 2021Quote: ... A dilution series ranging from 1.25 μg ml−1 to 10 μg ml−1 bovine serum albumin (BSA, Merck KGaA) was used for calibration and processed together with the samples.
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bioRxiv - Cancer Biology 2021Quote: ... cells in IMDM+Glutamax medium were cryopreserved in liquid nitrogen until time of analysis complemented 1:1 with 80% FCS and 20% dimethyl sulfoxide (DMSO) (Merck).
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bioRxiv - Cell Biology 2020Quote: 1×106 wt and hem1-/- DCs were spun down and lysed in 50µl 1x lysis buffer (1ml 10x RIPA buffer - NEB, 9ml H2O, 1 tablet PhosStop and 1 tablet protease inhibitor mini - both Merck). Lysates were spun at full speed for 10min at 4°C and supernatants transferred to new tubes ...
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bioRxiv - Developmental Biology 2021Quote: ... for 5 min at room temperature to loosen the zona pellucida and then treated with a hypotonic solution (1:1 mixture of 1.2% sodium citrate and 60% fetal bovine serum, FBS; Merck KGaA) for 10 min at room temperature ...
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bioRxiv - Bioengineering 2021Quote: The rat INS-1 832/3 cell line (insulinoma cell line stably transfected with human insulin; hereinafter INS-1) was obtained from Merck. A HUVEC (human umbilical vein endothelial cell ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were maintained under aseptic conditions in a 1:1 mixture of DMEM/F12 (DF-041-B; Merck Millipore) supplemented with 1% antibiotic/antifungal (15240062 ...
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bioRxiv - Bioengineering 2021Quote: ... The LCLs were maintained under aseptic conditions in a 1:1 mixture of Dulbecco’s modified Eagle medium and nutrient mixture F-12 (DMEM/F12; DF-041-B; Merck Millipore) supplemented with 1% antibiotic/antifungal (15240062 ...
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bioRxiv - Microbiology 2019Quote: ... Cultivations contained 1 mM L-tyrosine-13C9 (99% atom purity, Cortecnet) and 1 mM L-valine-13C5 (99% atom purity, Merck) and were conducted in four replicates at 37 °C and 180 rpm for 24 h ...
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bioRxiv - Biochemistry 2022Quote: ... Purified ShTnsC was concentrated to 1-2 mg mL−1 using 30,000 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Biophysics 2022Quote: HEK293T cells were cultured in 10 cm tissue culture treated dishes grown at 37 °C in a 5% CO2 atmosphere in HEPES buffered DMEM/F12 1:1 (Merck) supplemented with 10% fetal bovine serum and 2 mM L-glutamine ...
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bioRxiv - Immunology 2022Quote: ... and spleen were harvested and homogenated in PBS for CFU counting or in isotonic buffer (Tris HCl 50 nM, EDTA 2 mM, PMSF 1 mM [Roche Diagnostics GmbH], Triton X-100 1% [Merck Life Science] ...
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bioRxiv - Neuroscience 2019Quote: ... AB5543), chicken anti-β3 tubulin (1:500, AB9354) and mouse anti-ankyrin G (1:500, MABN466) were purchased from Merck Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... Purified ShTnsC was concentrated to 1-2 mg mL−1 using 30,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen ...
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bioRxiv - Genomics 2021Quote: ... The pellet was resuspended in PBS and the cells were then separated into three reactions: (1) added 1:20 volume of 1M NAI (03-310, Merck, 25 µl of NAI in 500 µl of infected cells ...
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bioRxiv - Immunology 2022Quote: Heparinised blood was diluted with PBS at a ratio of 1:1 and overlaid onto Histopaque 1083 (Sigma-Aldrich, Merck) density gradient medium using SepMate™-50 centrifugation tubes (Stem Cell Technologies) ...
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bioRxiv - Bioengineering 2022Quote: ... gels were washed for around 2-4 h with PBS and incubated in 1 ml 1 μM Alexa Fluor 546 NHS ester (NHS-AF546, #A20002, Merck), overnight at room temperature on a slowly rotating shaker ...
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bioRxiv - Bioengineering 2022Quote: ... Disruption buffer needed to be extensively washed away before adding 1 ml 1 μM Atto590-NHS ester (NHS-Atto590, #79636, Merck) in PBS to each gel ...