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Citations for Merck :
2451 - 2500 of 2532 citations for 3' Chloro 3 3 chloro 5 fluorophenyl 4' fluoropropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... The lysate was incubated for 2 hours at 4°C with 180 µl of anti-flag agarose beads (50 % slurry, ANTI-FLAG® M2 Affinity Gel, Merck, Kenilworth, NJ, USA), prewashed 3 × with 1 ml isotonic wash buffer (IsoWB ...
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bioRxiv - Bioengineering 2023Quote: ... The 1.5 ml resuspended RBCs were cultured in 50 ml HPS containing 2 mM CaCl2 and 2 µM calcium ionophore-4-bromo-A23187 (C7522, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) in T75 flasks in a 5% CO2 incubator at 37°C for 16 h ...
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bioRxiv - Neuroscience 2023Quote: ... 10-15 neurospheres and 4-6 organoids per cell line were rinsed twice with PBS and then lysed in RIPA buffer (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Microbiology 2023Quote: ... purified DNA from both input and ChIP fractions was diluted 1:4 in water and 1 μL was used for qPCR using a SYBR® Green JumpStart™ Taq ReadyMix™ (CAT S4438, Merck, UK) and a BioRad CFX96 instrument ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The crude radiolabeling mixture was purified through successive cycles of ultracentrifugation using Amicon Ultra-4 centrifugal filter units (Merck, Darmstadt, Germany, MWCO 30 kDa) with the addition of formulation buffer (5 mg/mL ascorbic acid in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μL of this complex was added to a mixture of 15 μL of TIRF-Casein buffer supplemented with 1 μL each of an oxygen scavenging system (4 mg/mL catalase, Merck; and 30 mg/mL glucose oxidase, Merck, dissolved in TIRF buffer), 45% (w/v ...
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bioRxiv - Cell Biology 2020Quote: U-2-OS Tet-On cell lines expressing eGFP-TopBP1 WT were grown under standard conditions (37°C, 5% CO2) in modified McCoy’s 5a medium (Merck-Sigma-Aldrich, M9309) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2021Quote: ... wells washed with 1 X PBS and incubated with 50 µL of incomplete media containing 5 µM Laurdan (Avanti® Polar Lipids, Merck, USA) for 30 min at 37°C ...
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bioRxiv - Genomics 2020Quote: ... C57BL/6 ESCs were transduced with the lentivirus at a multiplicity of infection (MOI) ranging from 0.1 to 1 with 5 μg/μL polybrene (Merck Millipore, Cat# TR1003G). From day three onward (e.g ...
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bioRxiv - Biochemistry 2021Quote: ... Pellets were thawn on ice and resuspended in lysis buffer (5 mL/g pellet: 50 mM Na2HPO4/KH2PO4, 150 mM NaCl, 0.2 % Tergitol™ solution (Merck, Darmstadt, Germany), and 2 mM DTT ...
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bioRxiv - Biochemistry 2021Quote: ... and subsequent thorough rinsing with ultrapure water (18.2 MΩ at 25 °C, maximum 2 ppb total organic carbon, Milli-Q Integral 5 Water Purification System, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Neuroscience 2022Quote: ... were randomly assigned to receive an injection of 0.9% NaCl v/v 5-bromo-2’Deoxyuridine (BrdU) 100 mg/kg (MERCK; New York, USA) three times within the same day ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μl of sample was injected in 50 % B and the analysis was performed using the gradient of 15 % A and 85 % B for 3.5 min followed by an increase to 75 % A and 25 % B over 11.5 min and its continuation for 10 min with the SeQuant® ZIC®-pHILIC column (5 µm, 150 mm x 2.1 mm, Merck, USA), at a flow rate of 0.13 ml/min.
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bioRxiv - Microbiology 2020Quote: ... water was purified by a Milli-Q system (resistivity 18.2 MΩ cm at 25°C, TOC < 5 ppb, Merck Millipore, Molsheim, France). Indole-3-acetic-2,2-d2 acid (≥ 98% ...
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bioRxiv - Neuroscience 2020Quote: ... half of the medium was replaced with fresh medium containing 20 μM of 5-fluorodeoxyuridine and uridine (Sigma Aldrich, Merck, Darmstadt, Germany) every 3 days ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The protein was concentrated overnight to a volume of 2 mL in a Vivaspin 20 Ultrafiltration Unit (5 kDa MWCO)(Merck, Darmstadt, Germany) and then loaded onto a HiLoadTM 26/60 Superdex TM 75 prep grade (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... mESCs were grown on gelatin-coated plates under standard culture conditions (37°C, 5% CO2, humid) atop a ‘feeder’ layer of MitomycinC-inactivated (Merck Life Science) SNLP mouse fibroblasts and passaged upon ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... 30 mL buffy coat was diluted with 5 mL DPBS (BioWhittaker®) and carefully poured over 15 mL of Histopaque® (Merck). After centrifugation (500 g ...
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bioRxiv - Plant Biology 2023Quote: ... The combined aqueous phase gave a total volume of 450 mL of which 5 uL were measured by Method 1 and compared against freshly prepared authentic standards (Merck, Darmstadt, Germany). We wish to note that our acid quantification generated much higher absolute values than an earlier study (Szecowka et al ...
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bioRxiv - Cell Biology 2023Quote: ... Protein transfer was then visualized using 0.1 % Ponceau S solution (w/v) in 5% acetic acid (Merck Life Sciences, Cat #: P7170-1L) and imaged ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A small aliquot of each sample was added to Hemi medium17 with a 5-μl/ml antibiotic cocktail (P4083, Merck, Darmstadt, Germany) and incubated under dark conditions at 19–20 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: CC strips were dissected and then transferred to microtiter plate wells containing 5 μM bis-N-methylacridinium nitrate (Lucigenin; Merck, Darmstadt, Germany), some strips were stimulated with NADPH (100 μM ...
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bioRxiv - Bioengineering 2023Quote: ... two thirds of the medium was removed from all wells and T cells were treated with 5 ng/mL polybrene (Merck, Darmstadt, Germany). Lentiviral particles (MOI=3 ...
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bioRxiv - Neuroscience 2024Quote: ... Postoperative care included antibiotic and analgesic administration (2.27% enrofloxacin, 5 mg/kg, Bayer, Kiel, Germany and 0.3 mg/ml buprenorphine, 8 µg/kg, Buprex, Merck & Co., Inc., NJ, USA) in a controlled temperature environment (37°C) ...
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bioRxiv - Microbiology 2024Quote: ... one stationary liquid culture was poured into a 50 mL syringe and plunged through a sterile 5 μm membrane filter (Millex-SV, Merck, Darmstadt, Germany), with slow and consistent pressure ...
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bioRxiv - Plant Biology 2024Quote: ... cinerea spores from glycerol stocks were diluted to a concentration of 5 x 104 spores/ml in ¼ PDB (Merck KGaA, Darmstadt, Germany) and pre-germinated for 4 hours at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Water was purified by a Milli-Q system (resistivity 18.2 MΩ cm at 25°C, TOC < 5 ppb, Merck Millipore, Molsheim, France). For laboratory culture samples ...
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bioRxiv - Cell Biology 2022Quote: ... protease inhibitor cocktail [5 μl/ml] and phosphatase inhibitor cocktail [10 μl/ml]) and 0.5% Nonidet P-40) (I8896; Sigma-Aldrich/Merck group, USA). After 10 min on ice ...
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bioRxiv - Cell Biology 2022Quote: ... Cell supernatant (50 mL) was then concentrated to 5 mL by centrifugation using Amicon® Ultra-15 Ultrafiltration Centrifuge Tubes (Millipore; Merck KGaA) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were left to soak for no less than 24 hours in either plain water (for the cryptic/plain treatment) or a 2.5% Bitrex™ aqueous solution (5 g denatonium benzoate (Merck, Darmstadt, Germany) dissolved into 200 ml water ...
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bioRxiv - Microbiology 2022Quote: ... solution containing 16 mM NaHCO3 and 0.15 mM ZnSO4 as well as on 5 mg/ml imipenem – 5 mg/ml cilastatin in 0.15 mM ZnSO4 prepared from the brand name Primaxin formulation (Merck, Sharp& Dohme Corp.) were also examined.
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bioRxiv - Microbiology 2022Quote: ... 0.5 ml of each aliquoted sample was filtered onto a 25 mm polycarbonate filter (pore size: 0.45 µm, Merck KGaA, Darmstadt, Germany). The filters were then air dried under sterile conditions and mounted onto 25mm diameter aluminum pin stubs (Agar Scientific Ltd. ...
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bioRxiv - Microbiology 2022Quote: ... the medium was replaced with complete medium without phenol red and 10 μL of 5 mg/mL MTT solution (In vitro toxicology assay kit, MTT-based, TOX-1KT, Merck, Darmstadt, Germany) were added to each well ...
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bioRxiv - Microbiology 2022Quote: ... Frozen larvae were thawed for two min in a solution of 50:50 5% bleach (Danklorix, CP GABA, Hamburg, Germany) and Milli-Q (Merck, Darmstadt, Germany) water for surface sterilization (Amiri et al. ...
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bioRxiv - Cancer Biology 2023Quote: A Thermo Ultimate 3000 high performance liquid chromatography (HPLC) system was equipped with a ZIC-pHILIC column (SeQuant; 150 mm by 2.1 mm, 5 μm; Merck KGaA, Darmstadt, Germany), with a ZIC-pHILIC guard column (SeQuant ...
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bioRxiv - Cell Biology 2023Quote: ... fixed cells were pelleted (1500 RPM 5 min) and resuspended in PBS containing 20 µg/ml propium iodide and 200 µg/ml RNase (Merck Life science).
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bioRxiv - Molecular Biology 2024Quote: ... The tissue was washed in PBS and incubated in 5 mM EDTA solution in PBS (pH 8; Merck Millipore, Burlington, MA, USA) at 4°C for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... the homogenized samples were incubated for 5 min at RT and 100 µL of liquid chloroform (Merck Millipore, Fontenay Sous Bois, France) were added ...
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bioRxiv - Cancer Biology 2024Quote: ... The stock solution was added at 1% v/v to Plasmax cell culture medium (CancerTools cat# 156371) supplemented with 5% FBS (Merck cat# F7524) and penicillin/streptomycin (Gibco cat# 15070063) ...
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bioRxiv - Cell Biology 2024Quote: ... The peak fractions were concentrated to approximately 5 mg/ml using an Amicon Ultra filter (Merck, molecular weight cut-off 10 kDa). The sample was subsequently supplemented with glycerol at a concentration of 10 %v/v ...
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bioRxiv - Developmental Biology 2024Quote: ... The females were stimulated 46 h prior to oocyte isolation using 5 IU of pregnant mare serum gonadotropin (PMSG; Folligon; Merck Animal Health) per mouse ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf tissues were cut into 5 mm-long x 2 mm-wide strips along the leaf proximodistal axis in a drop of water using a razorblade and immediately immersed in room temperature 4% w/v paraformaldehyde (pH6.9) (Merck Life Science UK Ltd., Gillingham, UK). Tissue was immediately placed in darkness and fixed at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... slices were washed two times with 1X Phosphate Buffer (30 mM Na2HPO4·12H2O [Merck, 10039-32-4]; 33 mM NaH2PO4 ·2H2O [Merck, 13472-35-0]; pH 7.4) and were dried on Superfrost (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... All the media were based on a M9 minimal medium composed of (i) M9 salts at pH = 7.2 : 47.76 mM Na2HPO4 (Merck/Sigma-Aldrich, CAS number : 7558-79-4), 22.04 mM KH2PO4 (Merck/Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated O/N at 4 °C with the following primary antibodies: mouse anti-glial fibrillary acidic protein (GFAP; Merck, Madrid, Spain; #MAB360; 1:1000) and rabbit anti-ionized calcium-binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were then washed 2x in Tween wash buffer (5 mM Tris-HCl [Sigma], 0.5 mM EDTA [AppliChem], 1 M NaCl [Merck], 0.05 % Tween-20 [Sigma]) and 1x in H2O ...
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bioRxiv - Biophysics 2020Quote: ... functionalized with either 100 μg/mL poly-D-lysine overnight for microglia cells or with 0.2 mg/mL fibronectin (FC010, Merck, 1:5 in PBS) for 2h at 37°C for fibroblasts ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were immersed in Neo-Clear® three times for 5 minutes and then mounted using Neo-mount® mounting medium (Merck Millipore), coverslipped and left to dry overnight in at 42°C.
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in blocking solution (5% bovine serum albumin in PBST) for 60 min at room temperature and then incubated with anti-CB1R (Merck Millipore, 1:1000) in blocking solution overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... After 72 hours of incubation at 33°C 5% CO2 wells were stained with Gram’s crystal violet solution (Merck KGaA, 64271 Damstadt, Germany) plus 5% formaldehyde 40% m/v (Carlo ErbaSpA ...