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Citations for Merck :
2201 - 2250 of 2532 citations for 3' Chloro 3 3 chloro 5 fluorophenyl 4' fluoropropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Chromatographic separation was performed on a SeQuant ZIC-HILIC column (5 µm, 200 Å, 150 × 2.1 mm, Merck, Germany) equipped with a SeQuant ZIC-HILIC guard column (20 × 2.1 mm ...
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bioRxiv - Biochemistry 2023Quote: ... 400 μg of AtLEGβ or papain were inhibited with 0.5 mM or 5 mM S-methyl methanethiosulfonate (MMTS, Merck), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin was enzymatically sheared for 5 min at 37°C with 0.2 mU/μL Micrococcal nuclease (MNase) (#N3755, Merck), and stopped with MNase stop buffer (110 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... CD3+ T cells were cultured in TexMACS Medium (Miltenyi Biotech) supplemented with 5% heat-inactivated human AB serum (Merck), 100 units/mL penicillin (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM K4Fe(CN)6 and 1 mg/ml X-gal (Roth)) and counterstained with nuclear fast red (Merck) for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... and SAM_Seq_Ex8_RV (5’-CTT CTT ATT GCC TCC TCT GGC ACA GC) together with KOD Hot Start DNA Polymerase (Merck) or KAPA HiFi HotStart ReadyMix ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were blocked with 5% BSA in TSMT (20 mM Tris; 150 mM NaCl, Merck; 1 mM CaCl2 (Sigma); 2 mM MgCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... resuspended in 20-100 µl 70% acetonitrile in water and run on a ThermoFisher Q-Exactive Orbitrap mass spectrometer equipped with Zic-pHILIC column (150×2.1 mm, 5 mm; Merck). A volume of 5 µl was injected and metabolites were monitored in full-scan ...
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bioRxiv - Molecular Biology 2023Quote: ... The formula was build based on a twofold serious dilution of glycerol (>/= 99% purification, Merck, cat # 56-81-5). The glycerol density (microgram glycerol/mg hyphal weight ...
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bioRxiv - Microbiology 2023Quote: ... Single colonies from those plates were inoculated in 5 mL of tryptic soy broth (TSB; Merck KGaA, Darmstadt, Germany) and incubated for 16 - 18 h at 37°C with shaking at 200 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... LC-MS analysis was performed with a ZIC-pHILIC chromatographic column (5 µm, 2.1 × 150 mm, Merck, Darmstadt, Germany). Full scan positive and negative ionization modes with a resolution of 70.000 (FWHM ...
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bioRxiv - Neuroscience 2023Quote: ... they were washed 3 times with 1X PBS for 5 minutes and incubated with DAPI (4’6-Diamidino-2-phenylindole; 1 µg/mL, MERCK) for 5 minutes ...
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bioRxiv - Immunology 2023Quote: Cells were infected separately with five different lentiviral transduction particles (at MOI = 5) containing five different shRNA species (Merck) specific for the mouse Flot2 gene (NM_008028 ...
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bioRxiv - Biophysics 2023Quote: ... The onion cells were prepared by dissecting a 5 mm x 5 mm section of the abaxial epidermis from a brown onion and mounting the membrane (5 mm x 5 mm) onto a #1.5 coverslip with 100 µl of neat Lugol’s iodine solution (62650; Merck, Germany) to produce a stained specimen.
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bioRxiv - Biochemistry 2023Quote: ... The proteins were reduced utilizing a 5 mM solution of tris-2-carboxyethyl-phosphine (TCEP) (Merck KGaA, Darmstadt, Germany) at a temperature of 60 °C for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... were separated using a ZIC-pHILIC guard and analytical column (SeQuant; 150 mm by 2.1 mm, 5 μm; Merck) on an Ultimate 3000 high-performance liquid chromatography (HPLC ...
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bioRxiv - Biochemistry 2024Quote: ... The chromatographic separation was performed on an Agilent Infinity II 1290 HPLC system using a SeQuant ZIC-pHILIC column (150 × 2.1 mm, 5 μm particle size, peek coated, Merck) connected to a guard column of similar specificity (20 × 2.1 mm ...
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bioRxiv - Immunology 2024Quote: B cell lines used as target cell lines (i.e. Raji and MEC1) were grown at 37°C and 5% CO2 in RMPI-1640 medium (Merck) supplemented with 7.5% iron-enriched BCS (GE Healthcare HyClone).
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bioRxiv - Immunology 2024Quote: ... followed by centrifugation at 1,000 rpm for 5 min and filtration using 0.45 μm sterile syringe filters (Merck Millipore). The filtrate was then concentrated with a 100 kD Amicon ultra centrifugal filter unit (Merck Millipore) ...
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bioRxiv - Neuroscience 2024Quote: ... whose composition is as plating media but with 5% FBS and 2 μM cytosine β-d-arabinofuranoside (Merck, C6645) to limit glial growth ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were preincubated for 1 h at RT in blocking buffer: 5% normal donkey serum (NDS, Vector) and 0.2 % Triton X-100 (Merck) diluted in PBS ...
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bioRxiv - Cancer Biology 2024Quote: Protein solutions were first diluted with 50 mM ammonium bicarbonate (ABC) and reduced with 5 mM dithiothreitol (DTT, Merck) at 60 °C for 45 min ...
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bioRxiv - Cell Biology 2024Quote: ... pH 8) and permeabilized and blocked with 1X TBS supplemented with 1% Triton-X100 (MERCK; Cat #9036-19-5) and 6% FBS (Biological Industries ...
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bioRxiv - Plant Biology 2024Quote: ... 0.5 g/l MES, Merck KGaA; pH 5.7; 0.7% phytoagar, Duchefa Biochemie; autoclaved; 25 µg/ml hygromycin, Merck KGaA). Plates with surface-sterilized seeds were stratified at 4°C in darkness for 2 days ...
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bioRxiv - Plant Biology 2024Quote: ... each probe (at a final concentration of 10 pM) was incubated in reaction buffer (5 mM CoCl2, 0.1 mM DIG-11-dUTP (Sigma Merck), 0.1 mM dATP ...
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bioRxiv - Molecular Biology 2024Quote: ... digested peptides were acidified by the addition of 5% (v/v) LC-MS grade Formid Acid (FA) (Merck, 5.33002.0050) and purified on C18 columns (Stagetips ...
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bioRxiv - Immunology 2024Quote: ... eggs were pelleted by centrifugation at 400 x g (5 minutes) and HES supernatant passed through a 0.22 μm syringe filter (Merck) and stored at -80°C.
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bioRxiv - Biochemistry 2024Quote: ... The cells were incubated at 37 °C in 5% CO2 for 60 min before addition of 0.5 ml SDS (Merck) solution (10%) ...
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bioRxiv - Developmental Biology 2024Quote: ... the lung distal regions were cut into small pieces and dissociated in 5 mL of enzyme mixture (0.125 mg/mL Collagenase, Merck, C9891 ...
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bioRxiv - Biochemistry 2024Quote: ... Staining was blocked for 30 min with CT buffer (0.5% Triton X-100, 5% ChemiBLOCKER in 0.1M NaP pH 7, Sigma Aldrich & Merck) at RT ...
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bioRxiv - Biochemistry 2024Quote: ... were lysed in 2 ml lysis buffer (0.5% IGEPAL, 100 mM KCl, 50 mM Tris pH 7.5, 5 mM MgCl2, with protease inhibitors (Merck, 4693159001)) ...
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bioRxiv - Biochemistry 2024Quote: ... cells were lysed in 500 μl lysis buffer (0.5% IGEPAL, 150 mM NaCl, 50 mM Tris-HCl pH 7.5, 5 mM MgCl2, protease inhibitors (Merck, 4693159001) and centrifugation 14,500 × g ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then lysed in 30 μl lysis buffer (0.5% IGEPAL, 150 mM NaCl, 50 mM Tris-HCl pH 7.5, 5 mM MgCl2, protease inhibitors (Merck, 4693159001) before centrifugation 14,500 × g ...
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Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... The lysates were supplemented with 5 mM CaCl2 and treated with 0.1 U/OD620 nm of Nuclease S7 (Merck) at 25°C with shaking at 400 rpm for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... Expanded and chopped gels were stored at 4°C in ddH2O immobilized prior to imaging on PDL-coated glass chambers (Merck, 734-2055).
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bioRxiv - Microbiology 2021Quote: Embedding in Lowicryl K4M (Chemische Werke Lowi, Waldkraiburg, Germany) was carried out on Vero cells fixed either in 4% formaldehyde (Merck, Darmstadt, Germany) or in 2% glutaraldehyde at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were fixed for 1 h at room temperature with 4% buffered formalin solution containing 1% crystal violet (Merck, Darmstadt, Germany). Finally ...
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bioRxiv - Immunology 2020Quote: Survival was determined by assessing the fraction of vital cells at defined time points during culture by flow cytometric exclusion of 4’,6-Diamidin-2-phenylindol (DAPI) (Merck, Darmstadt, Germany) and Annexin V-APC (BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 min each at RT and incubated with secondary antibodies in TPBS-TS-1% for 4h at 4°C (Alexa Fluor 546 anti-Mouse (Merck, A-11018), Alexa Fluor 488 anti-goat (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... spherical assembloids were collected in a PCR tube and immediately incubated in BBB working medium with 10 μM 4 kDa dextran-FITC (FD4; Merck, cat. #46944) or 10 μM fluorescently labeled human transferrin (Tf488 ...
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bioRxiv - Pathology 2022Quote: ... The sections were incubated overnight at 4°C in a pre-incubation solution containing mouse monoclonal em48 (Merck Millipore; MAB5374, 1:400) and rabbit polyclonal Laminin (Abcam ...
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Farnesyltransferase inhibition overcomes the adaptive resistance to osimertinib in EGFR-mutant NSCLCbioRxiv - Cancer Biology 2022Quote: ... fixed with paraformaldehyde 4 % solution for 10 min and stained with a solution containing PBS - 0.5% crystal violet (Sigma-Aldrich/Merck, ref: C3886) – 25% methanol for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... The cortices were homogenized mechanically in PBS by pipetting up and down with a 5mL plastic pipette, centrifuged (500 x g, 7 min, 4 °C) and resuspended in DMEM (Sigma-Aldrich, Merck, Switzerland) containing 5% FCS (Biochrom ...
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bioRxiv - Biochemistry 2020Quote: ... concentration and buffer exchange to TE with an Amicon Ultra-4 centrifuge filter unit (30 kDa cut-off, Merck Millipore, Cat#UFC803024).
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bioRxiv - Neuroscience 2020Quote: ... 0.1% (20mM Tris and 500mM NaCl pH 7.5) and incubated overnight at 4°C with the primary antibody: anti-GluN1 (1:1000; Merck KGaA, Darmstadt, Germany) and anti-GluN2A monoclonal antibody (1:1000 ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Immunology 2021Quote: ... These fractions were then pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096). The clinically relevant mAbs tested here were in-house produced versions of ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein containing fractions were pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096), as described previously (5).