Labshake search
Citations for Merck :
201 - 250 of 642 citations for SARS Coronavirus Nucleoprotein C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... Fixed cells were permeabilized with 0.01% saponin (10 minutes, 4°C) and labeled with Phalloidin-TRITC (Merck, 30 minutes, 4°C). After washing ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Single product was validated on 1.5% agarose gel after incubated at 95 °C for two minutes in thermo cycler with hot lid then brought to 22 °C and mixed with formamide (Merck millipore) in 1:3 ratio ...
-
bioRxiv - Molecular Biology 2020Quote: ... WT—or its mutants fused with a His-tag on the amino-terminus—were expressed in Escherichia coli BL21 (DE3) using pET15b vector (Merck). A single colony was inoculated in 2 mL Luria-Bertani broth (LB ...
-
bioRxiv - Cell Biology 2020Quote: ... pBADthio-LmaCK1.2ΔC43- V5-His6 and pBADthio-LmaCK1.2ΔN7-V5-His6 were sequenced and transformed in Escherichia coli Rosetta (DE3) pLysS Competent Cells (Merck Cat# 70956) for bacterial expression.
-
bioRxiv - Biochemistry 2021Quote: ... were transformed into Escherichia coli BL21-DE3-pLysS and expressed in 3 x 1 litre of Lucia Broth medium (Merck) supplemented with 100µg/ml ampicillin (Formedium) ...
-
bioRxiv - Cancer Biology 2020Quote: ... The glutathione-S-transferases (GST) fusion proteins were expressed in Escherichia coli and purified with glutathione-conjugated agarose beads (Merck) by standard procedures ...
-
bioRxiv - Microbiology 2023Quote: Atypical Enteropathogenic Escherichia coli O125ac:H6 (DSM8711) was purchased from the Leibniz Institute DSMZ and cultured in Luria-Bertani (LB) medium (Merck Millipore) and when necessary supplemented with kanamycin (50 μg/ml ...
-
bioRxiv - Neuroscience 2022Quote: ... ileum and colon sections were deparaffinated in histolene and rehydrated in a descending series of ethanol (100%, 96%, 70%) followed by hematoxylin solution (H&E, Merck, Darmstadt, Germany) for 5 min at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... as described above. The pretreatments with translational inhibitors puromycin (f. c. 20 µg/ml) and cycloheximide (f. c. 100 µg/ml) (Merck, Darmstadt, Germany) were performed after harvesting of the cells in the import buffer for 30 min at 37°C.
-
bioRxiv - Plant Biology 2022Quote: ... The lysate was cleared by two centrifugations (4000 rpm, 10 min, 4 °C and 20000g, 10 min, 4°C) and filtered (Miracloth, Merck 475855-1R). The lysate (input ...
-
bioRxiv - Physiology 2021Quote: ... and chemokine (C-X-C motif) ligand 1 (Cxcl1) were assessed using the MILLIPLEX-MAP kit Cytokine/Chemokine Magnetic Bead Panel (Merck Millipore, USA) according to the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2023Quote: ... The cell lysates were centrifuged at 16,000 x g for 10 minutes at 4°C and the supernatants were then incubated overnight at 4°C with anti-Flag M2 affinity gel beads (Merck, Cat.N. A2220). M2 beads were then pelleted and washed three times with lysis buffer containing protease inhibitors ...
-
bioRxiv - Biochemistry 2023Quote: Total cell lysates of HEK293 cells stably expressing C-terminally Flag M2-tagged SIRT4 were incubated overnight at 4°C with anti-Flag M2 agarose beads (Merck, Darmstadt, Germany). The beads were washed three times with washing buffer (50 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2019Quote: ... rabbit α-Tenascin C (Merck Millipore Cat. No. AB19013) 1:150 ...
-
bioRxiv - Neuroscience 2021Quote: ... anti-c-terminal APP (1:1000, rabbit, A8717, Merck), anti-APP/Aβ (1:1000 ...
-
bioRxiv - Microbiology 2021Quote: ... pET28a-c (cloning with NH2-terminal 6His tag, Merck), and pMal-c5x (cloning with the NH2-terminal maltose-binding protein (MBP ...
-
bioRxiv - Neuroscience 2021Quote: ... 2.1 × 150 mm at 15°C (Merck, Darmstadt, Germany), and on a UPLC Waters Acquity (Waters Corp ...
-
bioRxiv - Microbiology 2021Quote: ... Acetanilide (10.36 % N, 71.09 % C, Merck KGaA, Darmstadt, Germany) was used as the standard ...
-
bioRxiv - Microbiology 2023Quote: ... Drops of antifoam C emulsion (Merck Life Science NV), diluted six times ...
-
bioRxiv - Systems Biology 2023Quote: ... and 10 μL of IS C (Merck, Darmstadt, Germany) containing 0.40 nmol acylcoenzyme A (acyl-CoA ...
-
bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
-
bioRxiv - Biochemistry 2023Quote: ... Fusion proteins were expressed in Escherichia coli and subsequently purified using glutathione high-capacity magnetic agarose beads (Merck Millipore GmbH, Darmstadt, Germany) following the manufacturer’s guidelines.
-
bioRxiv - Neuroscience 2024Quote: ... each sample was separated on 5-20% gradient e-PAGEL mini gels (ATTO Corporation, Tokyo, Japan) and transferred to polyvinylidene fluoride membranes (Merck Millipore, Billerica, MA, USA). The membranes were incubated for 30 min with blocking buffer (Nacalai Tesque) ...
-
bioRxiv - Pathology 2021Quote: ... 4°C and filtered with a 0.45μm filter (Merck, Germany). MLNs were mashed by sliding two glass slides together ...
-
bioRxiv - Microbiology 2020Quote: ... c) Echinocandins: caspofungin (CPF, Merck & Co. Inc, Rahway, NJ, USA), micafungin (MCF ...
-
bioRxiv - Biochemistry 2023Quote: ... the C-terminus of H3 (Merck, 07-690, 1/3,000), H3K14ac (Merck ...
-
bioRxiv - Neuroscience 2023Quote: Add anti-mitotic agent cytosine arabinoside (Ara-C, Merck - C6645) to top compartment ...
-
bioRxiv - Cell Biology 2023Quote: ... and 2 µg of anti-c-myc (4A6; Merck Millipore). For immunoprecipitation of PER2::Luc constructs rabbit anti-firefly luciferase IgG (Abcam ...
-
bioRxiv - Neuroscience 2023Quote: ... anti-c- Fos (for chromogenic) (1:5000, ABE457,Merck, UK), anti-mCherry (1:2000 ...
-
bioRxiv - Biophysics 2024Quote: ... cell were treated with 5 µg/mL Mitomycin C (Merck) for 1 hour ...
-
bioRxiv - Cell Biology 2024Quote: ... rat neurons were plated at a density of 13 to 19 x103 cells/cm2 on glass coverslips coated with 333 µg/mL poly-D-lysine (PDL, Merck/Sigma-Aldrich #A-003-E). Mouse neurons were plated at a density of 13 to 19 x103 cells/cm2 on glass coverslips coated with 333 µg/mL PDL and 100 to 200 µg/mL laminin (Merck/Sigma-Aldrich #L2020) ...
-
bioRxiv - Microbiology 2022Quote: ... and/or Escherichia coli (ATCC 11303) were acclimated in a gas tight bottles (100 ml) containing Luria Bertani Broth media (LB, Merck Millipore, BD, 0083370) under anaerobic conditions (∼0.2 mg L-1 O2 ...
-
bioRxiv - Biophysics 2022Quote: ... coli cells were grown in M9 medium (Green et al., 2012; Meyer and Schlegel, 1983) containing (15NH4)2SO4 (Sigma/Merck KGaA, Darmstadt, Germany) or (15NH4)2SO4 and 13C-D-glucose (Euriso-Top GmbH ...
-
bioRxiv - Cell Biology 2019Quote: ... GST-Mm-RAB8A-Q67L and GST-Rn-RAB27A-Q78L proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore, Novagen, Darmstadt, Germany). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
-
bioRxiv - Microbiology 2019Quote: ... was grown at 37°C in LYBHI medium (BHI medium (Merck) supplemented with 0.5% yeast extract (Difco) ...
-
bioRxiv - Cell Biology 2022Quote: ... c-Src (Millipore Merck: 05-184, clone GD11, 1/1000 dilution), Ctsb (Atlas Antibodies ...
-
bioRxiv - Cell Biology 2021Quote: ... rabbit polyclonal Prosurfactant Protein C (1:200 pH9, AB3786, Merck Millipore), mouse monoclonal TTF-1 (clone SPT24 ...
-
bioRxiv - Biophysics 2020Quote: ... RNAse A (1 mg ml−1, 10 min, 21°C, Merck) or DNAse (200 Units well−1 ...
-
bioRxiv - Microbiology 2022Quote: ... mouse α-c-Myc antibodies (2.5 to 5 µg/ml; Merck), or mouse α-CSP 3D11 monoclonal antibodies (6 ...
-
bioRxiv - Microbiology 2023Quote: ... Isolates were grown overnight at 37°C on LB agar (Merck). Single colonies were inoculated into Mueller-Hinton broth (MHB ...
-
bioRxiv - Microbiology 2023Quote: ... under microaerophilic conditions in anaerobic jars with Anaerocult C (Merck, USA) at 37°C for 72 h ...
-
bioRxiv - Microbiology 2019Quote: ... a “spot test” was applied by plating 100 μL of a Escherichia coli ATCC® 25922 ™ overnight culture over a lawn of Tryptic Soy Agar (TSA, Merck Millipore™), and then adding 100 μL of the virus filtrate in each of three thirds of the plate.
-
bioRxiv - Microbiology 2022Quote: ... 55 mmol L-1 NaCl (Merck KGaA, pH 8.2; T = −27 °C). The mixture was immediately vortexed for 4 seconds and rapidly filtered through cellulose acetate filters (0.45 μm ...
-
bioRxiv - Genetics 2021Quote: ... elegans were maintained at 20°C on NGM plates (peptone, P4963, Merck; agar ...
-
bioRxiv - Microbiology 2023Quote: ... before imaging at 30 °C in Y04C microfluidic plates (Millipore Merck, UK) connected to an Onix microfluidic perfusion system (CellASIC Corp. ...
-
bioRxiv - Molecular Biology 2022Quote: ... Membranes were incubated with monoclonal anti-(c-Myc Tag) antibody (Merck Millipore) (HeLa extracts) ...
-
bioRxiv - Biochemistry 2023Quote: ... carbonic anhydrase (30 kDa) and cytochrome C (12 kDa; all from Merck). The relationship between elution volume and molecular mass determined using linear regression ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were maintained at 28°C with Ex-Cell 420 media (Merck), with shaking at 120 rpm for suspension culture and/or grown as static adherent cells ...
-
bioRxiv - Neuroscience 2019Quote: ... Blots were incubated with rabbit anti-C-terminus KCC2 (1:500, Merck Millipore) followed by horseradish peroxidise (HRP)-conjugated goat anti-rabbit antibody (1:2000 ...
-
bioRxiv - Plant Biology 2021Quote: ... but in frame with a C-terminal 6xHIS (Novagen, Merck KGaA, Darmstadt, Germany). This way ...