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Citations for Merck :
451 - 500 of 642 citations for SARS Coronavirus Nucleoprotein C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... and CM was concentrated to 1 ml at 4 °C using a 10 kDa Centricon Plus-70 centrifugal unit (Merck Millipore ...
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bioRxiv - Microbiology 2020Quote: ... washed with PBS at room temperature before permeabilized for 30 min in PBS containing 0.1% Triton X-100 and 10% normal goat serum and incubated overnight at 4 °C in PBS containing 10% normal goat serum and primary antibodies: anti-Cytokeratin 14 (K14) antibody (SP53; Merck), anti-Neurofilament heavy polypeptide (NF200 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... and incubated on an orbital shaker for 24h at room temperature + 72h at 4 °C in primary antibody (polyclonal rabbit anti-ERα, Merck-Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Samples were stored at 4°C overnight before desilicification with 4% suprapure hydrofluoric acid (Merck; incubation of approximately 5 hours). Afterwards ...
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bioRxiv - Biochemistry 2019Quote: ... A single colony of freshly transformed cells was cultured at 37 °C in 500 mL 2xYT medium supplemented with 50 µg/mL kanamycin (Merck) and 25 µg/mL chloramphenicol (Sigma Aldrich) ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... all proteins were centrifuged (16 000 g, 10 min, 20°C) and filtered (0.1 μm Ultrafree-MC filter, Merck Millipore). Proper performance of the MALS detectors was validated with bovine serum albumin ...
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bioRxiv - Molecular Biology 2021Quote: ... and the plates were incubated at 33 °C in a 5% CO2 atmosphere in fresh medium with 0.25% Hoechst (Merck-Millipore) to stain the DNA ...
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bioRxiv - Immunology 2021Quote: Culturing medium was changed 24 h post-transfection and vector supernatants were collected 48 h after transfection and processed by centrifugation at 1000 g for 10 mins at 4°C to remove cellular debris followed by microfiltration using Millex-HV 0.45 μm syringe filter units (Merck – SLHV033RB). Viral supernatants were either kept on ice for further use or frozen down at −80°C for storage.
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bioRxiv - Molecular Biology 2022Quote: Conditioned medium was centrifuged at 3,200xg for 10 min at 4°C to remove debris and transferred to 10 kDa spin columns (Amicon Ultra, Merck). Proteins that may influence lactate and pyruvate level were removed from the <10 kDa flow-through containing metabolites by centrifugation at 14,000xg for 25 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The whey supernatants were concentrated by centrifugation at 4,000 x g at 20°C using Amicon 100-kDa centrifugal filter units (Merck Millipore) to a final volume of ∼6 mL ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were washed and incubated overnight at 4°C with anti-Digoxigenin-AP antibody (45-11093274910 Merck-SIGMA, 1:1000). Staining was achieved by adding a solution of 4-Nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Molecular Biology 2022Quote: Cells were centrifuged at 3041 × g for 30 minutes at 4°C and supernatant was applied to a 500 mL Stericup-HV sterile vacuum filtration system (Merck) with a pore size of 0.22 µm ...
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bioRxiv - Microbiology 2023Quote: ... Clarification was then performed by centrifugation for 1 hour at 12,000g and 4°C and vacuum filtration using 45um nylon filter systems (SteriFlip - Merck Millipore). Prior to purification ...
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bioRxiv - Biochemistry 2022Quote: ... as described by the manufacturer and dried down in a Speed-Vac concentrator (Thermo-Scientific) resuspended in 20 μl L/C water containing 3% acetonitrile (MeCN) (Merck) and 0.1% FA ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 2 ml 4 °C PBS for 3 times and afterwards fixed in 1 ml of −20 °C cold methanol (Gradient grade for liquid chromatography, Merck) for 5 minutes at −20 °C ...
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bioRxiv - Microbiology 2022Quote: ... through six dilution and concentration steps at 4 °C using Amicon Ultra centrifugal filters with 10 kDa molecular weight cutoff (Merck). For experiments studying the IgG-binding properties of Fc-III and Scr ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS for ten minutes at 4 °C and then washed in 0.1% Tween20 in PBS (PBST) (Merck, Darmstadt, Germany) and blocked with 5% goat sera (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, #MRCPRT010 Ultracel YM-10). The mixture was centrifuged at 1000 rcf for 5 min ...
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bioRxiv - Immunology 2023Quote: ... for 24 to 48 hours to arrive at a mature BMDC culture or alternatively frozen to -80°C in 90% FCS (Biowest) and 10% DMSO (Merck) using a cell freezing container (CoolCell LX ...
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bioRxiv - Immunology 2023Quote: ... isolated cells from WAT were counted using cell counting chambers (one-way Neubauer counting chambers, C-Chip, Merck, Darmstadt, Germany). Single-cell suspensions were first stained with viability stain (Zombie NIR fixable viability kit ...
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bioRxiv - Neuroscience 2023Quote: ... heated to 37°C with a stock solution of gramicidin A (4 mg/ml - dissolved in dimethyl sulfoxide, DMSO, Merck) to achieve a final concentration of 80 μg/mL gramicidin79 ...
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bioRxiv - Cell Biology 2023Quote: ... Viral supernatants were harvested after at least 24 h of incubation at 32°C and filtered on a 0.45 µm filter (Merck #SE2M230I04).
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bioRxiv - Biochemistry 2023Quote: ... membrane using wet transfer for 3 h at 90 V/4 °C in transfer buffer (25 mM Tris; 192 mM glycine, Merck; 20% methanol, Merck). Afterwards ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was supplemented with calcium chloride to a final concentration of 3 mM and chromatin was solubilised for 2 h at 4 °C by digestion with Turbonuclease (T4330, Merck) to a final concentration of 250U/ml ...
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bioRxiv - Cell Biology 2023Quote: ... An aliquot of 40 µL was incubated (1h at 70°C on a heating block) by addition of 20 µL of the trimethylsilylating (TMSi) reagent (chlortrimethylsilane [Merck KGaA ...
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bioRxiv - Bioengineering 2023Quote: ... Then these samples were incubated in Alexa Fluor 488 conjugated mouse monoclonal anti-CTSK antibody (1:100) at 4°C overnight (Merck) and then for 1h under agitation at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... JKD6004 and the JKD6009/JKD6008 (VSSA/VISA) pair were routinely cultured at 37°C on solid or in liquid brain heart infusion (BHI, Merck) or Mueller-Hinton (MH ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryo and skin sections were then incubated with the primary antibodies overnight at 4°C (LHX2, 1:1000, #3030529 Merck, Darmstadt ...
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bioRxiv - Biochemistry 2023Quote: ... HeLa cells were cultivated at 37°C under 5% CO2 in MEM with Earle’s salts supplemented with 10% FBS superior (Merck KGaA), 2 mM L-Alanyl-L-Glutamine (Biochrom) ...
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bioRxiv - Neuroscience 2024Quote: Tissues were dissected out and homogenized at 4°C in RIPA buffer containing protease inhibitors cocktail (Merck Millipore, Milano, Italy) for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 250 µl of undiluted chromatin was incubated overnight at 4°C with Dynabeads M-270 Epoxy preincubated with anti-Tyr1P (MABE350, Merck), anti-Ser2P (C15200005-50 ...
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bioRxiv - Plant Biology 2024Quote: ... digestion of roots was performed at 90°C during 4 h in 400 µL of 65%(w/v) HNO3 (Suprapur, Merck). Mineralized samples were diluted in 0.65% (w/v ...
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bioRxiv - Immunology 2024Quote: ... They were then incubated for 10 minutes at 37°C with Liberase (250 μg/ml) and DNAse (50μg/ml Merck®). After incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times with PBS and boiled for 5 min at 99°C in hot reducing SDS-buffer containing 2-mercaptoethanol (Merck).
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bioRxiv - Microbiology 2024Quote: ... We stored them at -80 °C in phosphate buffered saline (PBS) with 20 % (v/v) glycerol (anhydrous; Merck, Darmstadt, Germany) and cultivated the isolates on Columbia blood agar plates containing 5 % sheep blood (Mast Diagnostica ...
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bioRxiv - Immunology 2024Quote: ... For intracellular cytokine labelling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (50ng/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated overnight at 4°C with primary antibodies (α-TOM20 Santa Cruz # sc-11415; α-STIM1 Merck WH0006786M1) diluted (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... or Tris-HCl buffered saline (TBS) and incubated at 4°C overnight with primary antibodies (anti-Nr4a2, Abcam, ab41917, 1:500 dilution; anti-GluA1, Merck-Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated in T cell medium for 30 min at 37°C without any metabolic inhibitors or in presence of 1 μM oligomycin (Merck), 100 mM 2DG (Merck) ...
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bioRxiv - Cell Biology 2024Quote: ... The next day the cleaved protein was collected and concentrated by spinning the sample at 3500 RPM at 4°C using −50kDa centrifugal filter tube (Amicon Ultra-15, Merck). The protein was purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE28-9909-44 ...
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bioRxiv - Immunology 2024Quote: ... NK cells were gently transferred to a 15-mL conical tube (Falcon) containing 8 mL of 4°C RPMI 1640 (ATCC modification) medium supplemented with 10 U/mL DNaseI (Merck). The cryovial was rinsed with 1 mL of the RPMI medium to transfer the remaining NK cells to the 15-mL tube ...
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bioRxiv - Immunology 2024Quote: ... The lentivirus particles were centrifuged onto the cells for 90min at 500g at 30°C in the presence of 4μg/ml polybrene (Sigma-Aldrich/Merck). Afterwards ...
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bioRxiv - Immunology 2023Quote: ... For intracellular cytokine labelling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (50ng/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Epiphyses were embedded in low melting agarose and tibias were placed for 30 min at 37°C in digestion medium composed of PBS with 3mg/ml of Collagenase B (C6885, Merck), 4mg/ml of Dispase (D4693 ...
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bioRxiv - Biophysics 2024Quote: ... Germany) for 4 h at 4°C followed by a washing in 0.1 M Soerensen’s Phosphate Buffer (Merck, Darmstadt, Germany). The dehydrating step was performed in the ascending alcohol series with 10 minutes duration each ...
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bioRxiv - Biophysics 2021Quote: ... Freshly excited slices were incubated for 30 min at 37°C in a solution of Nile Red (Cat. No. 72485-100MG, Sigma-Aldrich/Merck) pre-dissolved in methanol at a concentration of 1 mg/ml and diluted 1:20 in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Stag-Tulp3 was then pulled down from the TEV digestion eluates by overnight 4°C rotational incubation using S-protein agarose beads (Merck, #69704). Beads were then eluted with Laemmli buffer and processed for SDS-PAGE in Novex Value 4-20% Tris-Glycine gels (Thermofisher) ...
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bioRxiv - Cell Biology 2020Quote: Flp-In™T-REx™293 and HEK293 cell lines were grown under standard conditions (37°C, 5% CO2) in Dulbecco’s modified Eagle’s medium (Merck-Sigma-Aldrich, D5796). For Flp-In™T-REx™293 the medium was supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Developmental Biology 2021Quote: ... the following antibodies were treated to the organoids and the tissue slices at 4°C overnight: proSFTPC (1:200; Merck, AB3786), E-cadherin (1 ...
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bioRxiv - Developmental Biology 2021Quote: ... The tip epithelial cells were infected with the modified lentiviral vector for 24 h at 37°C in a single cell suspension in the SN medium containing 10 μM Y-27632 (Merck, 688000). After 24 h ...